Comparison of different diluents based on liposomes and egg yolk for ram semen cooling and cryopreservation

Document Type : Full paper (Original article)

Authors

1 Higher Education Unit Agricultural Technology and Marine Sciences, Center for Agricultural Technological Baccalaureate, No. 1, Coahuila, Mexico

2 Graduated from Postgraduate Department of Agricultural Production, Autonomous Agrarian University Antonio Narro, Coahuila, Mexico

3 Higher Education Unit Agricultural Technology and Marine Sciences, Center for Agricultural Technological Baccalaureate, No. 216, Durango, Mexico

4 Department of Animal Production, Autonomous Agrarian University Antonio Narro, Coahuila, Mexico

5 Department of Veterinary Sciences, Autonomous Agrarian University Antonio Narro, Coahuila, Mexico

Abstract

Background: Sperm cryopreservation is an important tool for breed improvement, nonetheless, spermatozoids of rams are extremely sensitive to cryopreservation. Aims: The present research was to compare a liposome-based (OptiXcell: OX) diluent, a commercial TRIS-egg yolk (Optidyl: OP) and a citrate egg yolk-based (CY) diluent on ovine semen quality through the cryopreservation process. Methods: Semen was collected from four sexually mature Dorper rams during the natural breeding season. After collection, semen was evaluated and diluted in OX, OP or CY diluent and was cooled from 37°C to 4°C for 2 h (refrigerated semen, RS), after that semen-filled straws were placed in liquid nitrogen (LN) vapour for 10 min, then immersed into LN at -196°C (cryopreserved semen, CS) and stored until evaluation. Results: For fresh semen (FS), similar values (P>0.05) were obtained from the 3 diluents [motility (4.2 ± 0.3), viability (75.4 ± 3.2), hypo-osmotic swelling test (HOST) (59.2 ± 2.1), and normality (84.7 ± 3.5)]. The motility values were higher for RS with OX and CY (4.0 ± 0.2 and 3.6 ± 0.3, respectively) compared to OP (3.0 ± 0.21; P<0.05). The viability was reduced after refrigeration and freezing (P<0.05). Refrigerated semen viability was similar for OX (65%), CY (63%) and OP diluents (60%; P>0.05), but for frozen semen, viability was lower in the CY diluent (P<0.05). Membrane integrity (HOST) in OX (53.6 ± 1.7) was similar to that in OP (50.7 ± 1.5; P>0.05) but higher than in CY (48.7 ± 1.5; P<0.05). Conclusion: No difference was found between the OX diluents and those made with egg yolk in terms of sperm parameters; however, the OX diluent was more efficient in protecting the integrity of membrane in freezing/thawing semen.

Keywords

References

Aisen, EG; Medina, VH and Venturino, A (2002). Cryopreservation and post-thawed fertility of ram semen frozen in different trehalose concentrations. Theriogenology. 57: 1801-1808.
Alcay, S; Toker, MB; Gokce, E; Ustuner, B; Onder, NT; Sagirkaya, H and Soylu, MK (2015). Successful ram semen cryopreservation with lyophilized egg yolk-based extender. Cryobiology. 71: 329-333.
Amirat, L; Tainturier, D; Jeanneau, L; Thorin, C; Gérard, O; Courtens, JL and Anton, M (2004). Bull semen in vitro fertility after cryopreservation using egg yolk LDL: a comparison with Optidyl®, a commercial egg yolk extender. Theriogenology. 61: 895-907.
Ansari, MS; Rakha, BA; Akhter, S and Ashiq, M (2016). OPTIXcell improves the postthaw quality and fertility of buffalo bull sperm. Theriogenology. 85: 528-532.
Arando, A; Gonzalez, A; Delgado, JV; Arrebola, FA and Perez-Marín, CC (2017). Storage temperature and sucrose concentrations affect ram sperm quality after vitrification. Anim. Reprod. Sci., 181: 175-185.
Asadpour, R; Jafari, R and Tayefi-Nasrabadi, H (2012). The effect of antioxidant supplementation in semen extenders on semen quality and lipid peroxidation of chilled bull spermatozoa. Iran. J. Vet. Res., 13: 246-249.
Belala, R; Briand-Amirat, L; Vinciguerra, L; Tainturier, D; Kaidi, R; Thorin, C and Bencharif, D (2016). Effect of equilibration time on the motility and functional integrity of canine spermatozoa frozen in three different extenders. Res. Vet. Sci., 106: 66-73.
Cerolini, S; Maldjian, A; Pizzi, F and Gliozzi, TM (2001). Changes in sperm quality and lipid composition during cryopreservation of boar semen. Reproduction. 121: 395-401.
CONAGUA (2015). Servicio Meteorológico Nacional. http://smn.cna.gob.mx/es/ informacion-climatologica-ver-estado?estado=coah. Consultado 14 Nov, 2018.
Fleisch, A; Malama, E; Witschi, U; Leiding, C; Siuda, M; Janett, F and Bollwein, H (2017). Effects of an extension of the equilibration period up to 96 hours on the characteristics of cryopreserved bull semen. Theriogenology. 89: 255-262.
Fonseca, JF; Torres, CAA; Maffili, VV; Borges, AM; Santos, ADF; Rodrigues, MT and Oliveira, RFM (2005). The hypoosmotic swelling test in fresh goat spermatozoa. Anim. Reprod., 2: 139-144.
Forouzanfar, M; Sharafi, M; Hosseini, SM; Ostadhosseini, S; Hajian, M; Hosseini, L and Nasr-Esfahani, MH (2010). In vitro comparison of egg yolk-based and soybean lecithin-based extenders for cryopreservation of ram semen. Theriogenology. 73: 480-487.
Garrett, FE; Goel, S; Yasul, J and Koch, RA (1999). Liposomes fuse with sperm cells and induce activation by delivery of impermeant agents. Biochim. Biophys. Acta Rev-Biomembr., 1417: 77-88.
Gharibi, S; Niasari-Naslaji, A; Poursasan, N and Moosavi-Movahedi, AA (2014). Replacement of salamon with shotor diluent and egg yolk with low density lipoprotein for chilled storage of ram semen. Iran. J. Vet. Res., 15: 279-284.
Gürler, H; Malama, E; Heppelmann, M; Calisici, O; Leiding, C; Kastelic, JP and Bollwein, H (2016). Effects of cryopreservation on sperm viability, synthesis of reactive oxygen species, and DNA damage of bovine sperm. Theriogenology. 86: 562-571.
Jerez, R; González, N; Olaciregui, M; Luño, V; de Blas, I and Gil, L (2016). Use of soy milk combined with different cryoprotectants for the ram semen cryopreservation. Small Rumin. Res., 134: 34-38.
Jiménez-Rabadán, P; Soler, AJ; Ramón, M; García-Álvarez, O; Maroto-Morales, A; Iniesta-Cuerda, M and Garde, JJ (2016). Influence of semen collection method on sperm cryoresistance in small ruminants. Anim. Reprod. Sci., 167: 103-108.
Kaeoket, K; Sang‐Urai, P; Thamniyom, A; Chanapiwat, P and Techakumphu, M (2010). Effect of docosahexaenoic acid on quality of cryopreserved boar semen in different breeds. Reprod. Domest. Anim., 45: 458-463.
Kafi, M; Safdarian, M and Hashemi, M (2004). Seasonal
variation in semen characteristics, scrotal circumference and libido of Persian Karakul rams. Small Rumin. Res., 53: 133-139.
Küçük, N; Aksoy, M; Uçan, U; Ahmad, E; Naseer, Z; Ceylan, A and Serin, I (2014). Comparison of two different cryopreservation protocols for freezing goat semen. Cryobiology. 68: 327-331.
Kumar, P; Saini, M; Kumar, D; Balhara, AK; Yadav, SP; Singh, P and Yadav, PS (2015). Liposome-based semen extender is suitable alternative to egg yolk-based extender for cryopreservation of buffalo (Bubalus bubalis) semen. Anim. Reprod. Sci., 159: 38-45.
Murphy, EM; O’Meara, C; Eivers, B; Lonergan, P and Fair, S (2018). Comparison of plant-and egg yolk-based semen diluents on in vitro sperm kinematics and in vivo fertility of frozen-thawed bull semen. Anim. Reprod. Sci., 191: 70-75.
Osuagwuh, UI and Palomo, MJ (2017). Effect of semen washing on thawed ram spermatozoa subjected to a four hour post-thawing thermal evaluation test. Small Rumin. Res., 155: 81-86.
Pillet, E; Labbe, C; Batellier, F; Duchamp, G; Beaumal, V; Anton, M and Magistrini, M (2012). Liposomes as an alternative to egg yolk in stallion freezing extender. Theriogenology. 77: 268-279.
Röpke, T; Oldenhof, H; Leiding, C; Sieme, H; Bollwein, H and Wolkers, WF (2011). Liposomes for cryopreservation of bovine sperm. Theriogenology. 76: 1465-1472.
Salamon, S and Maxwell, WMC (2000). Storage of ram semen. Anim. Reprod. Sci., 62: 77-111.
SAS (2005). Statistical Analysis System: A User’s Guide. Version 9.1, Statistical Analysis Systems Institute, Inc., Cary, NC., USA.
Stewart, JL; Shipley, CF; Ellerbrock, RE; Schmidt, L; Lima, FS and Canisso, IF (2018). Physiological variations in reproductive and metabolic features of white-tailed deer (Odocoileus virginianus) bucks throughout the rutting season. Theriogenology. 114: 308-316.
Swami, DS; Kumar, P; Malik, RK; Saini, M; Kumar, D and Jan, MH (2017). Cysteamine supplementation revealed detrimental effect on cryosurvival of buffalo sperm based on computer-assisted semen analysis and oxidative parameters. Anim. Reprod. Sci., 177: 56-64.
Taghilou, P; Rostami, B; Masoumi, R and Mirzaei-Alamouti, H (2017). Effects of supplementation of the Tris-egg yolk extender with n-3 polyunsaturated fatty acids (PUFAs) on frozen-thawed ram semen characteristics. Small Rumin. Res., 155: 1-5.
Valente, SS; Pereira, RM; Baptista, MC; Marques, CC; Vasques, MI; Pereira, MS and Barbas, JP (2010). In vitro and in vivo fertility of ram semen cryopreserved in different extenders. Anim. Reprod. Sci., 117: 74-77.
Yang, DH; Standley, NT and Xu, ZZ (2018). Application of liquid semen technology under the seasonal dairy production system in New Zealand. Anim. Reprod. Sci., 194: 2-10.
Vásquez, J; Florentini, EA; Camargo, LA; Gonzales, J and Valdivia, M (2013). Hypoosmotic swelling test in epididymal ram (Ovis aries) spermatozoa. Livest. Sci., 157: 618-622.

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