Effect of protected-glutamine supplementation on performance, milk composition and some blood metabolites in fresh Holstein cows

Document Type : Short paper


1 Department of Microbiology, Faculty of Veterinary Sciences, Ilam Univeristy, Ilam, Iran

2 Department of Animal Science, Faculty of Agriculture, Ilam Univeristy, Ilam, Iran

3 Department of Animal Science, Faculty of Agriculture, Univeristy of Zanjan, Zanjan, Iran


The present study was conducted to study the effect of protected-glutamine (Gln) supplementation on dry matter intake (DMI), milk yield (MY) and composition, somatic cell counts (SCC) and blood parameters in fresh cows. Forty Holstein cows at zero day of parturition (calving day = day 0) were divided into four groups (n=10), and fed (ad libitum) with one of the diets including: basal diet (control), basal diet supplemented with 150 (low Gln, LG), 250 (medium Gln, MG) or 350 (high Gln, HG) g of Gln protected with formaldehyde/cow per day. The DMI and MY were recorded from 0 to 21 days post-calving. Milk fat and protein were assessed on days 7, 14 and 21, and blood was collected on days 0, 7, 14, and 21 after parturition. The DMI and MY at 21 days in milk (DIM) in HG group were compared with control (P<0.05). The DMI at 14 and 21 DIM and the MY at 21 DIM were higher in MG group compared with control group (P>0.05). Glucose concentration at 7, 14 and 21 DIM increased in both HG and MG groups compared with control group (P>0.05). The milk SCC of Gln groups was lower (P<0.05) compared with control, at 14 and 21 DIM. Glutamine supplementation increased the blood concentrations of total protein and albumin, but lowered the β-hydoxybutyrate (BHBA), non-esterified fatty acids (NEFA) and aspartate amino transferase (AST) concentrations (P<0.05). These results indicate that rumen protected Gln supplementation at 250 g/heat/day to fresh Holstein cows improved the SCC in milk and health status.


AOAC (1995). Official methods of analysis 16th Edn., Association of official analytical chemists. Washington D.C., USA.
Ardawi, MSM and Newsholme, EA (2001). Glutamine metabolism in lymphocytes of the rat. Biochemis. J., 212: 835-842.
Bell, AW (1995). Regulation of organic nutrient metabolism during transition from late pregnancy to early lactation. J. Anim. Sci., 73: 2804-2819.
Bobe, G; Young, JW and Beitz, DC (2004). Invited review:
pathology, etiology, prevention, and treatment of fatty liver in dairy cows. J. Dairy Sci., 87: 3105-3124.
Brown, WE and Allen, MS (2013). Effects of intrajugular glucose infusion on feed intake, milk yield, and metabolic responses of early postpartum cows fed diets varying in protein and starch concentration. J. Dairy Sci., 96: 7123-7142.
Calsamiglia, S; Ferret, A; Reynolds, CK; Kristensen, NB and Van Vuuren, AM (2010). Strategies for optimizing nitrogen use by ruminants. Animal. 47: 1184-1196.
Caroprese, M; Albenzio, M and Sevi, A (2013). Dietary glutamine enhances immune responses of dairy cows under high ambient temperature. J. Dairy Sci., 96: 1-10.
Doepel, L; Lobley, GE and Lapierre, H (2007). Effect of glutamine supplementation on splanchnic metabolism in lactation dairy cows. J. Dairy Sci., 90: 4325-4333.
Duffield, TF; Lissemore, KD; McBride, BW and Leslie, KE (2009). Impact of hyper-ketonemia in early lactation dairy cows on health and production. J. Dairy Sci., 92: 571-580.
Edmonson, AJ; Lean, IJ; Weaver, LD; Farver, T and Webster, G (1989). A body condition scoring chart for Holstein dairy cows. J. Dairy Sci., 72: 68-78.
Jafari, A; Emmanuel, DGV; Christopherson, RJ; Thompson, JR; Murdoch, GK; Woodward, J; Field, CJ and Ametaj, BN (2006). Parenteral administration of glutamine modulates acute phase response in postparturient dairy cows. J. Dairy Sci., 89: 4660-4668.
Lohrenz, AK; Duske, K; Schneider, F; Nürnberg, K; Losand, B and Seyfer, HM (2010). Milk performance and glucose metabolism in dairy cows fed rumen-protected fat during mid lactation. J. Dairy Sci., 93: 5867-5876.
Maeda, Y; Ohtsuka, H and Oikawa, M (2012). Effect of the periparturient period on blood free amino acid concentration in dairy cows/healthy cows. J. Vet. Med. Anim. Health. 4: 124-129.
McGeough, EL; Okiely, P and Kenny, DA (2010). A note on the evaluation of the acid-insoluble ash technique as a method for determining apparent diet digestibility in beef cattle. Irish J. Agr. Food Res., 49: 159-164.
Meijer, GA; Van der Meulen, J and Van Vuuren, AM (1993). Glutamine is a potentially limiting amino acid for milk production in dairy cows: a hypothesis. Metabolism. 42: 358-364.
Meyer, DJ and Harvey, JW (1998). Evaluation of hepatobiliary system and skeletal muscle and lipid disorders. Veterinary laboratory medicine. Interpretation and diagnosis. 2nd Edn., Philadelphia, London, Toronto, Montreal, Sydney, Tokyo, W. B. Saunders Company. PP: 157-187.
NRC (2001). Nutrient requirements of dairy cattle. 7th Edn., Washington, D.C., National Academies Press.
Plaizier, JC; Walton, JP and Mcbride, BW (2001). Effect of post-ruminal infusion of glutamine on plasma aminoacids, milk yield and composition in lactating dairy cows. Can. J. Anim. Sci., 81: 229-235.
Roche, JR; Kay Jane, K; Friggens, NC; Loor, JJ and Berry, DP (2013). Assessing and managing body condition score for the prevention of metabolic disease in dairy cows. Vet. Clin. Food Anim., 29: 323-336.
SAS (2003). SAS Users Guide. SAS Institute Inc., Cary, NC, USA.
Texeira, NM; Freitas, AF and Barra, RB (2003). Environmental factors influencing monthly somatic cell counts in herds of the state of Minas Gerais. Arq. Bras. Med. Vet. Zootec., 55: 491-499.