Comparative therapeutic efficacy of levofloxacin, ornidazole and alpha tocopherol combination with prostaglandin F2α on IL-6 and IL-10 transcript level in longstanding cases of endometritis in crossbreed Jersey cows

Document Type : Full paper (Original article)

Authors

1 MVSc in Animal Reproduction, Gynaecology and Obstetrics, Department of Animal Reproduction, Gynaecology and Obstetrics, College of Veterinary Science & Animal Husbandry, Orissa University of Agriculture and Technology, Bhubaneswar, 751003, India

2 Ph.D. Scholar in Veterinary Gynaecology and Obstetrics, Department of Veterinary Gynaecology and Obstetrics, ICAR-National Dairy Research Institute, Karnal, Haryana, 132001, India

3 Ph.D. Scholar in Animal Genetics and Breeding, Department of Animal Genetics & Breeding, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati-781022, India

4 Department of Teaching Veterinary Clinical Complex, College of Veterinary Science & Animal Husbandry, Orissa University of Agriculture and Technology, Bhubaneswar, 751003, India

5 Department of Veterinary Physiology, College of Veterinary Science & Animal Husbandry, Orissa University of Agriculture and Technology, Bhubaneswar, 751003, India

6 ICAR-Project Directorate on Foot-and-Mouth Disease, Mukteshwar, Nainital, Uttarakhand, 263138, India

7 Department of Animal Reproduction, Gynaecology and Obstetrics, College of Veterinary Science & Animal Husbandry, Orissa University of Agriculture and Technology, Bhubaneswar, 751003, India

Abstract

This study compared the therapeutic efficacy of levofloxacin, ornidazole and alpha tocopherol combination and prostaglandin F2α (PGF2α) in longstanding cases of endometritis and evaluated their impact on Interleukin-6 (IL-6) and Interleukin-10 (IL-10) transcript level in peripheral blood leukocytes. Eighteen endometritic crossbred Jersey cows were randomly allotted to three groups (six in each) viz. Group I (levofloxacin combo treatment I/U), group II (PGF2α treatment I/M), group III (no treatment, control), and group IV (six non-endometritic healthy cyclic) was taken for comparison study. The clinical efficacy was assessed by haematological study (TLC: Total leukocyte count; DC: Differential count), polymorphonuclear leukocytes (PMN) count in uterine cytology and relative mRNA expression of IL-6 and IL-10 in peripheral blood leukocytes before and after treatment with respect to conception rate following single and second inseminations. Group I and II registered significant increase in TLC and neutrophil count. PMN cytology was increased two and three fold in group I and II, respectively. The IL-6 transcript level was increased by 2.5 and 4.6 fold while that of IL-10 increased by 3.7 and 5.2 fold in group I and II, respectively. Conception rate across group I to IV following single insemination was found to be 66.67%, 50%, 16.67%, and 83.33% and their corresponding values following second insemination were 66.67%, 83.33%, 16.67%, and 83.33%, respectively. Thus, the administration of levofloxacin combo and PGF2α might have better conception rate following first and second insemination, respectively. Our study also reveals that PGF2α could register better clearance of bacteria through stronger PMN cell and cytokine activity in post-treatment period.

Keywords

References

Ahmad, I; Gohar, A; Ahmad, N and Ahmad, M (2003). Haematological profile in cyclic, non cyclic and endometritic cross bred cattle. Int. J. Agric. Biol., 5: 332-334.
Barlund, CS; Carruthers, TD; Waldner, CL and Palmer, CW (2008). A comparison of diagnostic techniques for postpartum endometritis in dairy cattle. Theriogenology. 69: 714-723.
Benjamin, MM (1979). Outline of veterinary clinical pathology. 3rd Edn., Iowa, USA, The StateUniversity Press Ames.
Biswal, SS; Das, S; Mohanty, DN and Jena, D (2014). Effect of systemic and local immunomodulation therapies on conception rate in endometritic cows. Ind. J. Field Vet., 10: 1-4.
Brodzki, P; Kostro, K; Brodzki, A; Wawron, W; Marczuk, J and Kurek, L (2015). Inflammatory cytokines and acute-phase proteins concentrations in the peripheral blood and uterus of cows that developed endometritis during early postpartum. Theriogenology. 84: 11-18.
Butterfield, TA; Best, TM and Merrick, MA (2006). The dual roles of neutrophils and macrophages in inflammation: a critical balance between tissue damage and repair. J. Athl. Train., 41: 457-465.
Chapwanya, A; Meade, KG; Doherty, ML; Callanan, JJ; Mee, JF and O’Farrelly, C (2009). Histopathological and molecular evaluation of Holstein-Friesian cows post-partum: toward an improved understanding of uterine innate immunity. Theriogenology. 71: 1396-1407.
Chauhan, RS (1995). Text book of veterinary clinical and laboratory diagnosis. 1st Edn., Ltd., Delhi, Lordson Publishers (P).
Duvel, A; Maaß, J; Heppelmann, M; Hussen, J; Koy, M; Piechotta, M; Sandra, O; Smith, DG; Sheldon, IM; Dieuzy-Labaye, I; Zieger, P and Schuberth, HJ (2014). Peripheral blood leukocytes of cows with subclinical endometritis show an altered cellular composition and gene expression. Theriogenology. 81: 906-917.
Fischer, C; Drillich, M; Odau, S; Heuwieser, W; Einspanier, R and Gabler, C (2010). Selected pro-inflammatory factor transcripts in bovine endometrial epithelial cells are regulated during the oestrous cycle and elevated in case of sub-clinical or clinical endometritis. Reprod. Fertil. Dev., 2: 818-829.
Foldi, J; Kulcsar, M; Pecsi, A; Huyghe, B; De Sa, C; Lohuis, JA; Cox, P and Huszenicza, G (2006). Bacterial complications of postpartum uterine in cattle. Anim. Reprod. Sci., 96: 265-281.
Galvao, KN; Frajblat, M; Brittin, SB; Butler, WR; Guard, CL and Gilbert, RO (2009). Effect of prostaglandin F2α on subclinical endometritis and fertility in dairy cows. J. Dairy Sci., 92: 4906-4913.
Galvao, KN; Santos, NR; Galvao, JS and Gilbert, RO (2011). Association between endometritis and endometrial cytokine expression in postpartum Holstein cows. Theriogenology. 76: 290-299.
Gilbert, RO; Shin, ST; Guard, CL; Erb, HN and Frajblat, M (2005). Prevalence of endometritis and its effects on reproductive performance of dairy cows. Theriogenology. 64: 1879-1888.
Heidarpour, M; Mohri, M; Fallah-Rad, AH; Shahreza, FD and Mohammadi, M (2014). Hematological changes before and after treatment in dairy cows with clinical and subclinical endometritis. Comp. Clin. Path., 23: 97-101.
Hogarth, PJ (1982). Immunological aspects of mammalian reproduction. 1st Edn., Anybook Ltd., Lincoln, United Kingdom., Springer Publishers.
Islam, R; Kumar, H; Nandi, S and Rai, RB (2013). Determination of anti-inflammatory cytokine in periparturient cows for prediction of postpartum reproductive diseases. Theriogenology. 79: 974-979.
Kasimanickam, R; Duffield, TF; Foster, RA; Gartley, CJ; Leslie, KE; Walton, JS and Johnson, WH (2004). Endometrial cytology and ultrasonography for the detection of subclinical endometritis in postpartum dairy cows. Theriogenology. 62: 9-23.
Kasimanickam, R; Duffield, TF; Foster, RA; Gartley, CJ; Leslie, KE; Walton, JS and Johnson, WH (2005). A comparison of the cytobrush and uterine lavage techniques to evaluate endometrial cytology in clinically normal postpartum dairy cows. Can. Vet. J., 46: 255-259.
Kumar, M; Pant, SS; Ram, R; Kumar, S and Gupta, PK (2014). Therapeutic efficacy of levofloxacin along with vitamin E for the management of repeat breeding syndrome in cow under field condition. Int. J. Vet. Sci., 3: 155-157.
LeBlanc, SJ (2008). Postpartum uterine disease and dairy herd reproductive performance: a review. Vet. J., 176: 102-114.
Livak, KJ and Schmittgen, TD (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCt Method. Methods. 25: 402-408.
Mateus, L; da Costa, LL; Bernardo, F and Silva, JR (2002). Influence of puerperal uterine infection on uterine involution and postpartum ovarian activity in dairy cows. Reprod. Domest. Anim., 37: 31-35.
Nino-Soto, MI; Heriazon, A; Quinton, M; Miglior, F; Thompson, K and Mallard, BA (2008). Differential gene expression of high and low immune responder Canadian Holstein dairy cows. Dev. Biol., 132: 315-320.
Noakes, DE; Parkinson, TJ and England, GCW (2001). Arthur’s veterinary reproduction and obstetrics. 8th Edn., W.B. Saunders. P: 864.
Pathan, MM; Das, H; Khan, MJZ; Siddiquee, GM; Latif, A and Parsani, HR (2011). Comparative studies on haemato-biochemical profile of cyclic and non-cyclic Holstein-Friesian cross-bred cows, Waymba. J. Anim. Sci. e-journal. ISSN: 2012-578X.
Patra, MK; Kumar, H and Nandi, S (2014). Differential cytokine expression profile in peripheral blood mononuclear cells of endometritic buffaloes. Indian J. Anim. Sci., 84: 1265-1269.
Radostits, OM; Gay, CC; Hinchcliff, KW and Constable, PD (2007). Veterinary medicine. 10th Edn., Philadelphia, USA, W. B. Saunders. P: 2065.
Reddy, NCS; Bramhaiah, KV; Naidu, KS and Suresh Kumar, RV (2012). Effect of uterine lavage therapy on haematological and biochemical parameters in repeat breeder cows. Therio. Insight. 2: 1-6.
Sahoo, S; Mohanty, DN; Das, S and Padhy, A (2014). Effect of uterine immunomodulation on hematobiochemical parameters in cyclic non-breeding cows. Vet. World. 7: 816-820.
Sarma, DK; Singh, B; Singh, MP; Tiwary, BK and Sinha, MP (2012). Therapeutic use of immunomodulators in endometritic cows and their effect on WBC and RBC indices. Intas. Polivet., 13: 26-28.
Schalm, OW (1965). Clinical hematology for the horse and cow. Mod. Vet. Pract., 46: 52-53.
Sharma, S and Dhaliwal, GS (2009). Escherichia coli lipopolysaccharide induced immunomodulation along with oxytocin administration after mating as a treatment protocol for persistent endometritis in mares. J. Equine. Vet. Sci., 30: 259-265.
Sheldon, IM; Cronin, J; Goetze, L; Donofrio, G and Schuberth, HJ (2009). Defining postpartum uterine disease and the mechanism of infection and immunity in the female reproductive tract in cattle. Biol. Reprod., 81: 1025-1032.
Sheldon, IM and Dobson, H (2004). Postpartum uterine health in cattle. Anim. Reprod. Sci., 82-83: 295-306.
Singh, B; Singh, KP; Singh, SV; Singh, JP and Singh, HN (2011). Efficacy of intrauterine use of levofloxacin and alpha tocopherol on conception rate in repeat breeder crossbred cows. Indian Vet. J., 88: 72-73.
Snedecor, GW and Cochran, WG (1994). Statistical methods. 8th Edn., USA, LowStateUniversity Press.
Zemjanis, R (1970). Diagnostic and therapeutic techniques in animal reproduction. 2nd Edn., North Miami Beach, FL, U.S.A., Harcourt Publishers., Himeographed Paper Presented at New England Vet. Meeting.

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