Comparative therapeutic efficacy of levofloxacin, ornidazole and alpha tocopherol combination with prostaglandin F2α on IL-6 and IL-10 transcript level in longstanding cases of endometritis in crossbreed Jersey cows

Document Type : Full paper (Original article)


1 MVSc in Animal Reproduction, Gynaecology and Obstetrics, Department of Animal Reproduction, Gynaecology and Obstetrics, College of Veterinary Science & Animal Husbandry, Orissa University of Agriculture and Technology, Bhubaneswar, 751003, India

2 Ph.D. Scholar in Veterinary Gynaecology and Obstetrics, Department of Veterinary Gynaecology and Obstetrics, ICAR-National Dairy Research Institute, Karnal, Haryana, 132001, India

3 Ph.D. Scholar in Animal Genetics and Breeding, Department of Animal Genetics & Breeding, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati-781022, India

4 Department of Teaching Veterinary Clinical Complex, College of Veterinary Science & Animal Husbandry, Orissa University of Agriculture and Technology, Bhubaneswar, 751003, India

5 Department of Veterinary Physiology, College of Veterinary Science & Animal Husbandry, Orissa University of Agriculture and Technology, Bhubaneswar, 751003, India

6 ICAR-Project Directorate on Foot-and-Mouth Disease, Mukteshwar, Nainital, Uttarakhand, 263138, India

7 Department of Animal Reproduction, Gynaecology and Obstetrics, College of Veterinary Science & Animal Husbandry, Orissa University of Agriculture and Technology, Bhubaneswar, 751003, India


This study compared the therapeutic efficacy of levofloxacin, ornidazole and alpha tocopherol combination and prostaglandin F2α (PGF2α) in longstanding cases of endometritis and evaluated their impact on Interleukin-6 (IL-6) and Interleukin-10 (IL-10) transcript level in peripheral blood leukocytes. Eighteen endometritic crossbred Jersey cows were randomly allotted to three groups (six in each) viz. Group I (levofloxacin combo treatment I/U), group II (PGF2α treatment I/M), group III (no treatment, control), and group IV (six non-endometritic healthy cyclic) was taken for comparison study. The clinical efficacy was assessed by haematological study (TLC: Total leukocyte count; DC: Differential count), polymorphonuclear leukocytes (PMN) count in uterine cytology and relative mRNA expression of IL-6 and IL-10 in peripheral blood leukocytes before and after treatment with respect to conception rate following single and second inseminations. Group I and II registered significant increase in TLC and neutrophil count. PMN cytology was increased two and three fold in group I and II, respectively. The IL-6 transcript level was increased by 2.5 and 4.6 fold while that of IL-10 increased by 3.7 and 5.2 fold in group I and II, respectively. Conception rate across group I to IV following single insemination was found to be 66.67%, 50%, 16.67%, and 83.33% and their corresponding values following second insemination were 66.67%, 83.33%, 16.67%, and 83.33%, respectively. Thus, the administration of levofloxacin combo and PGF2α might have better conception rate following first and second insemination, respectively. Our study also reveals that PGF2α could register better clearance of bacteria through stronger PMN cell and cytokine activity in post-treatment period.


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