جداسازی مولکولی و تعیین خصوصیات ویروس منقار و پر طوطی سانان در طوطی سانان تهران، ایران

نوع مقاله : مقاله کامل

نویسندگان

چکیده

ویروس پر و منقار طوطی سانان (BFDV)، یک ویروس کوچک، بدون پوشش و دارای DNA تک رشته‌ای می‌باشد که در جنس سیرکوویروس‌ها قرار می‌گیرد. اگرچه بیشترین مطالعات به روی سیرکوویروس‌ها به روی این ویروس بوده، اطلاعات این ویروس در ایران اندک می‌باشد. هدف از این مطالعه شناسایی ویروس در موارد ارجاعی به کلینیک دانشکده دامپزشکی دانشگاه تهران می‌باشد. در این مطالعه تعداد 55 نمونه متعلق به نه گونه از طوطی سانان جهت بررسی رخداد بیماری اخذ شد. نمونه‌های اخذ شده با آزمون واکنش زنجیره‌ای پلیمراز (PCR) جهت تشخیص ژن rep از ویروس مورد نظر ارزیابی مولکولی شدند. پس از انجام آزمون واکنش زنجیره‌ای پلیمراز، تعداد ده نمونه متعلق به چهار گونه (ملوسیتاکوس آندولتس (4)، سیتاکولا کرامری (3)، سیتاکوس اریتاکوس (2)، پلاتیسرکوس اگزیمیوس (1)) به لحاظ وجود ویروس مثبت بودند. در ارزیابی فیلوژنیک ویروس‌های مثبت، در چهار شاخه جدا از هم متعلق به سویه‌های مختل فاز ویروس L1)، N1، T1 و (I4 قرار گرفتند. موارد مثبت به لحاظ حساسیت میزبانی و جغرافیای مورد بحث قرار گرفتند. با این وجود مطالعات بیشتری در این زمینه و میزان شیوع این بیماری مورد نیاز می‌باشد.

کلیدواژه‌ها


Bassami, MR; Berryman, D; Wilcox, GE and Raidal, SR (1998). Psittacine beak and feather disease virus nucleotide sequence analysis and its relationship to porcine circovirus, plant circoviruses and chicken anaemia virus. J. Virol., 249: 453-459.
Bassami, MR; Ypelaar, I; Berryman, D; Wilcox, GE and Raidal, SR (2001). Genetic diversity of beak and feather disease virus detected in psittacine species in Australia. J. Virol., 279: 392-400.
Bert, E; Tomassone, L; Peccati, C; Navarrete, MG and Sola, SC (2005). Detection of beak and feather disease virus (BFDV) and avian polyomavirus (APV) DNA in psittacine birds in Italy. J. Vet. Med. B Infect. Dis. Vet. Public Health. 52: 64-68.
De Kloet, E and de Kloet, SR (2004). Analysis of the beak and feather disease viral genome indicates the existence of several genotypes which have a complex psittacine host specificity. Arch. Virol., 149: 2393-2412.
Ha, HJ; Anderson, IL; Alley, MR; Springett, BP and Gartrell, BD (2007). The prevalence of beak and feather disease virus infection in wild populations of parrots and cockatoos in New Zealand. N. Z. Vet. J., 55: 235-238.
Hakimuddin, F; Abidi, F; Jafer, O; Li, C; Wernery, U; Hebel, Ch and Khazanehdari, K (2015). Incidence and detection of beak and feather disease virus in psittacine birds in the UAE. Biomol. Detect.Quantif., 11: 27-32.
Halami, MY; Nieper, H; Müller, H and Johne, R (2008). Detection of a novel circovirus in mute swans (Cygnus olor) by using nested broad-spectrum PCR. Vir. Res., 132: 208-212.
Hattermann, K; Schmitt, C; Soike, D and Mankertz, A (2003). Cloning and sequencing of duck circovirus (DuCV). Arch. Virol., 148: 2471-2480.
Heath, L; Martin, DP; Warburton, L; Perrin, M; Horsfield, W; Kingsley, C; Rybicki, EP and Williamson, AL (2004). Evidence of unique genotypes of beak and feather disease virus in Southern Africa. J. Virol., 78: 9277-9284.
Hsu, CM; Ko, CY and Tsaia, HJ (2006). Detection and sequence analysis of avian polyomavirus and psittacine beak and feather disease virus from psittacine birds in Taiwan. Avian Dis., 50: 348-353.
Hughes, AL and Piontkivska, H (2008). Nucleotide sequence polymorphism in circoviruses. Infect. Genet. Evol., 8: 130-138.
Jing, G; Tian, J; Tan, X; Yu, H; Ding, S; Sun, H and Yu, X (2011). Pathological observations of an experimental infection of geese with goose circovirus. Avian Pathol., 40: 55-61.
Johne, R; Fernandez-de-Luco, D; Hofle, U and Müller, H (2006). Genome of a novel circovirus of starlings, amplified by multiply primed rolling-circle amplification. J. Gen. Virol., 87: 1189-1195.
Johne, R; Raue, R; Grund, C; Kaleta, EF and Müller, H (2004). Recombinant expression of a truncated capsid protein of beak and feather disease virus and its application in serological tests. Avian Pathol., 33: 328-336.
Julian, L; Piasecki, T; Chrzastek, K; Walters, M; Muhire, B; Harkins, GW; Martin, DP and Varsani, A (2013). Extensive recombination detected among beak and feather disease virus isolates from breeding facilities in Poland. J. Gen. Virol., 94: 1086-1095.
Khalesi, B; Bonne, N; Stewart, M; Sharp, M and Raidal, SA (2005). Comparison of haemagglutination, haemagglu-tination inhibition and PCR for the detection of psittacine beak and feather disease virus infection and a comparison of isolates obtained from loriids. J. Gen. Virol., 86: 3039-3046.
Maclachlan, NJ; Edward, JD and Fenner, F (2011). Fenner’s veterinary virology. 4th Edn., Amsterdam: Elsevier/AP. PP: 237-241.
Mansoori, J (2013). A guide to the birds of IRAN. 3rd Edn., Tehran: Farzaneh Books. P: 279.
Mészáros, I; Tóth, R; Bálint, A; Dán, A; Jordan, I and Zádori, Z (2014). Propagation of viruses infecting waterfowl on continuous cell lines of Muscovy duck (Cairina moschata) origin. Avian Pathol., 43: 379-386.
Nei, M and Kumar, S (2000). Molecular evolution and phylogenetics. 1st Edn., Oxford, UK, Oxford University Press.
Paré, JN and Robert, N (2007). Circovirus. In: Thomas, NJ; Hunter, DB and Atkinson, CT (Eds.) Infectious diseases of wild birds. (1st Edn.), Ames, IA: Blackwell Pub., PP: 194-205.
Phenix, KV; Weston, JH; Ypelaar, I; Lavazza, A; Smyth, JA; Todd, D; Wilcox, GE and Raidal, SR (2001). Nucleotide sequence analysis of a novel circovirus of canaries and its relationship to other members of the genus circovirus of the family Circoviridae. J. Gen. Virol., 82: 2805-2809.
Rahaus, M and Wolff, MH (2003). Psittacine beak and feather disease: a first survey of the distribution of beak and feather disease virus inside the population of captive psittacine birds in Germany. J. Vet. Med. B Infect. Dis. Vet. Public Health. 50: 368-371.
Rampin, T; Manarolla, G; Pisoni, G; Recordati, C and Sironi, G (2006). Circovirus inclusion bodies in intestinal muscle cells of a canary. Avian Pathol., 35: 277-279.
Raue, R; Johne, R; Crosta, L; Bürkle, M; Gerlach, H and Müller, H (2004). Nucleotide sequence analysis of a C1 gene fragment of psittacine beak and feather disease virus amplified by real-time polymerase chain reaction indicates a possible existence of genotypes. Avian Pathol., 33: 41-50.
Rinder, M; Schmitz, A; Peschel, A and Korbel, R (2015). Complete genome sequence of a novel circovirus from zebra finch. Genome Announc., 3(3): e00560-15. doi:
10.1128/genomeA.00560-15.
Ritchie, PA; Anderson, IL and Lambert, DM (2003). Evidence for specificity of psittacine beak and feather disease viruses among avian hosts. J. Virol., 306: 109-115.
Robino, P; Grego, E; Rossi, G; Bert, E; Tramuta, C; Stella, MC; Bertoni, P and Nebbia, P (2014). Molecular analysis and associated pathology of beak and feather disease virus isolated in Italy from young Congo African grey parrots (Psittacus erithacus) with an “atypical peracute form” of the disease. Avian Pathol., 43: 333-344.
Sarker, S; Ghorashi, SA; Forwood, JK and Raidal, SR (2013). Whole-genome sequences of two beak and feather disease viruses in the endangered swift parrot (Lathamus discolor). Genome Announc., 1: e00842-e00913.
Schmidt, RE and lightfood, TL (2006). Integument. In: Harrison, GJ and Lightfoot, TL (Eds.), Clinical avian medicine. (1st Edn.), Palm Beach, FL: Spix Pub., PP: 399-401.
Schmidt, RE; Reavill, DR and Phalen, DN (2015). Pathology of pet and aviary birds. 2nd Edn., Ames, Iowa: Blackwell Pub., PP: 178-179, 248-250.
Shearer, PL; Sharp, M; Bonne, N; Clark, P and Raidal, SR (2009). A blocking ELISA for the detection of antibodies to psittacine beak and feather disease virus. J. Virol. Methods. 158: 136-140.
Soike, D; Kohler, B and Albrecht, K (1999). A circovirus-like infection in geese related to a runting syndrome. Avian Pathol., 28: 199-202.
Stewart, ME; Perry, R and Raidal, SR (2006). Identification of a novel circovirus in Australian ravens (Corvus coroboides) with feather disease. Avian Pathol., 35: 86-92.
Tajima, F and Nei, M (1984). Estimation of evolutionary distance between nucleotide sequences. Mol. Bio. Evol., 1: 269-285.
Tamura, K; Stecher, G; Peterson, D; Filipski, A and Kumar, S (2013). MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Mol. Bio. Evol., 30: 2725-2729.
Todd, D (2000). Circoviruses: immunosuppressive threats to avian species: a review. Avian Pathol., 29: 373-394.
Todd, D; Duchatel, JP; Weston, JH; Ball, NW; Borghmans, BJ; Moffett, DA and Smyth, JA (2002). Evaluation of polymerase chain reaction and dot blot hybridization tests in the diagnosis of pigeon circovirus infections. Vet. Microbiol., 89: 1-16.
Todd, D; Fringuelli, E; Scott, ANJ; Borghmans, BJ; Duchatel, JP; Shivaprasad, HL; Raidal, SR; Abadie, JX; Franciosini, MP and Smyth, JA (2008). Sequence comparison of pigeon circoviruses. Res. In. Vet. Sci., 84: 311-319.
Todd, D; Scott, ANJ; Fringuelli, E; Shivraprasad, HL; Gavier-Widen, D and Smyth, JA (2007). Molecular characterization of novel circoviruses from finch and gull. Avian Pathol., 36: 75-81.
Varsani, A; Regnard, GL; Bragg, R; Hitzeroth, II and Rybicki, EP (2011). Global genetic diversity and geographical and host-species distribution of beak and feather disease virus isolates. J. Gen. Virol., 92: 752-767.
Woods, LW; Latimer, KS; Barr, BC; Niagro, FD; Campagnoli, RP; Nordhausen, RW and Castro, AE (1993). Circovirus-like infection in a pigeon. J. Vet. Diag. Invest., 5: 609-612.
Ypelaar, I; Bassami, MR; Wilcox, GE and Raidal, SR (1999). A universal polymerase chain reaction for the detection of psittacine beak and feather disease virus. Vet. Microbiol., 68: 141-148.