Comparative efficacy of serological diagnostic methods and evaluation of polymerase chain reaction for diagnosis of bovine brucellosis

Document Type : Short paper

Authors

1 MVSc Scholar, Department of Veterinary Epidemiology and Preventive Medicine, U. P. Pt. Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura-281001, Uttar Pradesh, India

2 Department of Veterinary Epidemiology and Preventive Medicine, U. P. Pt. Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura-281001, Uttar Pradesh, India

3 Department of Veterinary Microbiology, U. P. Pt. Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura-281001, Uttar Pradesh, India

Abstract

The aim of present study was to compare the diagnostic performance of the different Brucella abortus antigen based serological and molecular tests such as Rose Bengal Plate Test (RBPT), indirect enzyme linked immunosorbent assay (I-ELISA) and polymerase chain reaction (PCR). Out of 89 samples, 44 were positive by I-ELISA, 18 by RBPT and 21 by PCR. Substantial agreement was observed between PCR and I-ELISA (κ=0.48). A slight degree of agreement was observed between RBPT and I-ELISA and PCR (κ=0.18) and RBPT and PCR (κ=0.11). Indirect enzyme linked immunosorbent assay detected more samples as positive among these tests. In conclusion, I-ELISA can be routinely used for an accurate and efficient diagnosis of Brucella infection, because the chances of non-detection of an infected animal in I-ELISA are minimal. However, PCR could be used as a supplement and complement test along with I-ELISA for identification and differentiation of bovine brucellosis.

Keywords

References

Al-Garadia, MA; Khairani-Bejo, S; Zunita, Z and Omar, AR (2011). Detection of Brucella melitensis in blood samples collected from goats. J. Anim. Vet. Adv., 10:
1437-1444.
Alton, GG; Jones, LM and Piezt, DE (1975). Laboratory techniques in brucellosis. 2nd Edn., Monograph Series No. 55, World Health Organization (WHO) Geneva. PP: 112-113.
Bricker, BJ and Halling, SM (1994). Differentiation of Brucella abortus bv. 1, 2 and 4 Brucella melitensis, Brucella ovis, and Brucella suis bv. 1 by PCR. J. Clinc. Microbiol., 32: 2660-2666.
Çiftci, A; Ica, T; Savaşan, S; Sareyyupoglu, B; Akan, M and Diker, KS (2017). Evaluation of PCR methods for detection of Brucella strains from culture and tissues. Trop. Anim. Health Prod., 49: 755-763.
Ghorbani, A; Khorasgani, MR; Zarkesh-Esfahani, H; Sharifiyazdi, H; Kashani, AD and Emami, H (2013). Comparison of serology, culture, and PCR for detection of brucellosis in slaughtered camels in Iran. Comp. Clin. Pathol., 22: 913-917.
Kaushik, P; Singh, DK; Tiwari, AK and Kataria, RS (2006). Rapid detection of Brucella sppin cattle by PCR. J. Appl. Anim. Res., 30: 25-28.
Kaynak-Onurdag, F;Okten, S andSen, B (2016). Screening Brucella spp. in bovine raw milk by real-time quantitative PCR and conventional methods in a pilot region of vaccination, Edirne, Turkey. J. Dairy Sci., 99: 3351-3357.
Kumar, A; Gupta, VK; Verma, AK; Sahzad; Kumar, V; Singh, A and Reddy, NCP (2016). Seroprevalence and risk factors associated with bovine brucellosis in western Uttar Pradesh, India. Ind. J. Anim. Sci., 86: 131-135.
Kumar, N; Pal, BC; Yadav, SK; Verma, AK; Jain, U and Yadav, G (2009). Prevalence of bovine brucellosis in Uttar Pradesh, India. J. Vet. Pub. Hlth., 7: 129-131.
Mittal, V; Kumar, M and Ambwani, T (2005). Sero-epidemiological pattern of brucellosis among livestock of district Udham Singh Nagar in Uttaranchal. Ind. J. Vet. Med., 25: 28-32.
Neha; Ahmed, W; Verma, AK; Jain, U and Bist, B (2014). Brucellosis in organized dairy farm: an investigation. Asian J. Anim. Sci., 8: 29-33.
OIE (2012). Bovine brucellosis. Section 2.3. 5th Edn., In OIE Manual of Standards for Diagnostic Tests and Vaccines. OIE, Paris.
Sambrook, J and Russell, DW (2001). Molecular cloning: a laboratory manual. (3rd Edn.), New York, ColdSpringHarbor Laboratory Press. PP: 545-547.
Thrusfield, M (2008). Veterinary epidemiology. 3rd Edn., UK, Blackwell Publishing Ltd., PP: 311-316.
Ul Islam, MR; Gupta, MP; Sidhu, PK; Filia, G; Saxena, HM and Shafi, TA (2013). Comparative evaluation of indirect enzyme linked immunosorbentassay, rose bengal plate test, microagglutination test, and polymerase chain reaction for diagnosis of brucellosis in buffaloes. Turk. J. Vet. Anim. Sci., 37: 1-5.
Verma, AK; Dhama, K; Chakraborty, S; Kumar, A; Tiwari, R; Rahal, A; Mahima and Singh, SV (2014). Strategies for combating and eradicating important infectious diseases of animals with particular reference to India: present and future perspectives. Asian J. Anim. Vet. Adv., 9: 77-106.
Zerva, K; Bourantas, S; Mitka, K and Legakis, NJ (2001). Serum is the preferred clinical specimen for diagnosis of human brucellosis by PCR. J. Clin. Microbiol., 39: 1661-1664.

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