Study of TALP and TRIS citrate medium on caprine sperm capacitation and subsequent in vitro embryo production

Document Type : Short paper

Authors

1 Ph.D. Scholar in Biotechnology, Department of Biotechnology, Institute of Applied Science and Humanities, GLA University, Mathura, Uttar Pradesh, India

2 Animal Physiology and Reproduction Division, ICAR-Central Institute for Research on Goats (CIRG), Makhdoom, Farah-281122, Mathura, Uttar Pradesh, India

3 Department of Biotechnology, Institute of Applied Science and Humanities, GLA University, Mathura, Uttar Pradesh, India

Abstract

The aim of the present investigation was to compare Tyrode’s albumin lactate pyruvate (TALP) medium and TRIS citrate medium for capacitation of caprine sperm. In experiment 1 capacitation was assessed by chlortetracycline assay and in experiment 2 with in vitro fertilization and embryo development. In experiment 2, cumulus oocyte complexes (COCs) recovered by slicing the caprine ovaries were matured in maturation medium for 27 h in humidified atmosphere at 38.5°C with 5% CO2. After 27 h of culture a total of 2480 in vitro matured oocytes were selected and randomly divided into two groups. Group 1 (n=1124) matured oocytes were fertilized by the spermatozoa capacitated in TALP medium and in group 2 (n=1356) matured oocytes were fertilized by the spermatozoa capacitated in TRIS citrate medium. The results of experiment 1 indicated a comparatively more number of sperms with Chlortetracycline (CTC) Pattern B in TRIS citrate than TALP medium (55.32 ± 0.91% vs 47.96 ± 0.20%). In experiment 2, the cleavage rate and blastocyst production were higher following capacitation of spermatozoa in TRIS citrate than TALP medium. In conclusion, TRIS citrate can be used as an alternative and effective media for sperm capacitation to get higher cleavage rate and blastocyst production in goat.

Keywords

References

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