Comparing three superovulation protocols in dromedary camels: FSH, eCG-FSH and hMG

Document Type : Full paper (Original article)


1 Graduated from Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

2 Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

3 BVSc in Animal Science, Camel Advanced Reproductive Technologies Center, Government of Dubai, Dubai, UAE


The objective of this study was to compare three superovulation protocols in dromedary camels. Follicular waves of dromedary camel donors (n=6) were synchronized using two GnRH injections. Superovulation was initiated 2 days after the second injection (day 0 of experiment). The experiment was conducted using change over design, where all females received three superovulation treatments one after the other with a resting period of one month in between. Superovulation was carried out for all donors using 390 mg FSH, 1000 IU eCG and 330 mg FSH, or 16.5 ampule hMG (75 i.u. FSH and 75 i.u. LH per ampoule). FSH and hMG were injected twice daily, indecreasing doses, over 5.5 days. Thirty-six h after the last FSH or hMG injection, donors were mated once and received an intravenous injection of the GnRH analogue. The diameter and number of ovarian follicles ≥4 mm on day 4 after superovulation and the total number of corpora lutea and follicles ≥9 mm on day 7.5 after mating were evaluated by ultrasound examinations. No significant differences were found between the total number of corpora lutea among FSH (13.8 ± 2.65), eCG-FSH (15 ± 2.60) and hMG (10.8 ± 2.30) and the number of expanded hatched blastocysts in FSH (5.7 ± 2.32), eCG-FSH (8.8 ± 2.10) and hMG (5.8 ± 2.40) treated donors. This study showed that all three superovulatory protocols could be used successfully and interchangeably in dromedary camels.


Anouassi, A and Ali, A (1990). Embryo transfer in camel (Camelus dromedarius). In: Saint-Martin, G (Ed.), Proceedings of the workshop: Is it possible to improve the reproductive performance of the camel?, Paris, 10-12 September 1990. PP: 327-332.
Anouassi, A and Tibary, A (2013). Development of a large commercial camel embryo transfer program: 20 years of scientific research. Anim. Reprod. Sci., 136: 211-221.
Cole, HH; Bigelow, M; Finkel, J and Rupp, GR (1967). Biological half-life of endogenous PMS following hysterectomy and studies on losses in urine and milk. Endocrinology. 81: 927-930.
Cooper, MJ; Skidmore, J; Ali, M; Billah, T; Wensvoort, S; Billah, AA and Allen, WR (1990). An attempt to induce and synchronise ovulation and superovulation in dromedary camels for embryo transfer. In: Saint-Martin, G (Ed.), Proceedings of the workshop: Is it possible to improve the reproductive performance of the camel?, Paris, 10-12 September 1990. PP: 313-326.
Laster, DB (1972). Disappearance and uptake of [125I] FSH in the rat, rabbit, ewe and cow. J. Reprod. Fertil., 30: 407-415.
Lauria, A; Genazzani, AR; Oliva, O; Inaudi, P; Cremonesi, F; Monittola, C and Aureli, G (1982a). Clinical and endocrinological investigations on superovulation induced in heifers by human menopausal gonadotrophin. J. Reprod. Fertil., 66: 219-225.
Lauria, A; Oliva, O; Genazzani, AR; Cremonesi, F; Crotti, S and Barbetti, M (1982b). Improved method to induce superovulation in cattle using human menopausal gonadotropin (HMG). Theriogenology. 18: 357-364.
McGowan, MR; Braithwaite, M; Jochle, W and Maplecoft, RJ (1985). Superovulation of beef heifers with Pergonal (HMG): a dose response trial. Theriogenology. 24: 173-184.
McIntosh, JEA; Moor, RM and Allen, WR (1975). Pregnant mare serum gonadotrophin: rate of clearance from the circulation of sheep. J. Reprod. Fertil., 44: 95-100.
McKinnon, AO and Tinson, AH (1992). Embryo transfer in dromedary camels. In: Allen, WR; Higgins, AJ; Mayhew, IG; Snow, DH and Wade, JF (Eds.), Proceedings of the first international camel conference. Dubai, 2-6 February 1992. R &W Publications (Newmarket) Ltd., PP: 203-208.
McKinnon, AO; Tinson, AH and Naion, G (1994). Embryo transfer in dromedary camels. Theriogenology. 41: 145-150.
Merkt, H; Rath, D; Musa, B and El-Nagger, MA (1990). Reproduction in camels. FAO Animal Production and Health. No: 82, FAO, Rome.
Moghiseh, A; Niasari-Naslaji, A; Nikjou, D; Gerami, A; Razavi, K and Mostafaey, M (2008). The effect of LH and GnRH analogues on induction of ovulation in Bactrian camel (Camelus bactrianus). Iran. J. Vet. Res., 9: 324-329.
Morell, AG; Gregoriadis, G; Scheinberg, IH; Hickman, J and Ashwell, G (1971). The role of sialic acid in determining the survival of glycoproteins in the circulation. J. Biol. Chem., 246: 1461-1467.
Musa, BE; Siema, H; Merkt, H; Hago, B; Cooper, M; Allen, W and Jochle, W (1993). Manipulation of reproductive functions in male and female camels. Anim. Reprod. Sci., 33: 289-306.
Nowshari, MA and Ali, SA (2005). Effect of season and gonadotropins on the superovulatory response in camel (Camelus dromedarius). Theriogenology. 64: 1526-1535.
SAS (2014). Statistical Analysis System: A User’s Guide, Version 9.4, Cary, NC.
Schams, D; Mentzer, C; Schallenberger, E; Hahn, J and Hahn, R (1978). Some studies of pregnant mare serum gonadotropin and on endocrine responses after application for superovulation in cattle. In: E.E.C. Seminar: Control of Reproduction in the Cow. PP: 122-143.
Skidmore, JA; Allen, WR; Cooper, MJ; Chaudhry, MA; Billah, M and Billah, MA (1992). The recovery and transfer of embryo in the dromedary camel: results of preliminary experiments. In: Allen, WR; Higgins, AJ; Mayhew, IG; Snow, DH and Wade, JF (Eds.), Proceedings of the First International Camel Conference. Dubai, 2-6 February 1992. R &W Publications (Newmarket) Ltd., PP: 137-142.
Skidmore, J and Billah, M (2011). Embryo transfer in the dromedary camel (Camelus dromedarius) using non-ovulated and ovulated, asynchronous progesterone-treated recipients. Reprod. Fertil. Dev., 23: 438-443.
Skidmore, JA; Billah, M and Allen, WR (2002). Investigation of factors affecting pregnancy rate after embryo transfer in the dromedary camel. Reprod. Fertil. Dev., 14: 109-116.
Tibary, A; Anouassi, A and Sghiri, A (2005). Factors affecting reproductive performance of camels at the herd and individual level. Desertification combat and food safety: The Added Value of Camel Producers (NATO Science Series). Amsterdam, Netherlands, IOS Press. PP: 97-114.
Tinson, A; Singh, K and Kuhad, KS (2000). Large scale management of camels for embryo transfer. J. Camel Prac. Res., 7: 143-147.
Vyas, S; Singh, AK; Goswami, P; Bissa, UK; Rai, AK and Khanna, ND (1998). Superovulation and non-surgical embryo flushing in Indian camel (Camelus dromedarius). Int. J. Anim. Sci., 13: 147-148.