Prevalence of Campylobacter jejuni and Campylobacter coli in captive wildlife species of India

Document Type : Full paper (Original article)


1 Division of Veterinary Public Health, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly-243 122, Uttar Pradesh, India

2 Division of Bacteriology and Mycology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly-243 122, Uttar Pradesh, India

3 Division of Pathology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly-243 122, Uttar Pradesh, India

4 Department of Veterinary Parasitology, Madras Veterinary College, TANUVAS, Chennai-600007, Tamilnadu, India


Campylobacteriosis is an important zoonotic disease and the prevalence of Campylobacter is largely unknown in the wildlife of India. A total of 370 samples, comprising of 314 fresh faecal samples from apparently healthy captive wild animals and birds, 30 stool swabs from animal care takers and 26 samples of the animals’ food and water were collected from G. B. Pant High Altitude Zoo, Nainital, Kanpur Zoo, Wildlife Park, IVRI and the Post Graduate Research Institute in Animal Sciences (PGRIAS), Chennai, Tamilnadu from August 2014 to May 2015. Samples were processed for cultural isolation, direct PCR and multiplex PCR for species confirmation. To decipher the genetic diversity, the 16S rRNA gene was amplified, sequenced and analyzed. Based on isolation, the overall occurrence rate of Campylobacter spp. was 0.8% (3/370), being 2.94% (3/102) for captive wild birds. Three Campylobacter jejuni were isolated from silver pheasants, lady amherest pheasants and saras cranes. Direct PCR assay showed the overall occurrence rate of Campylobacter spp. to be 4.77% (15/315), being 1.58% (2/126) for captive wild ruminants, 5.81% (5/86) for non-ruminants and 7.84% (8/102) for birds. All the isolates were identified as C. jejuni.


Adesiyun, AA; Caeser, K and Inder, L (1998b). Prevalence of Salmonella and Campylobacter infections in animals at Emperor Valley Zoo, Trinidad, American Association of Zoo Veterinarians. J. Zoo Wildl. Med., 29: 237-239.
Adesiyun, AA; Seepersadsingh, N; Inder, L and Caesar, K (1998a). Some bacterial enteropathogens in wildlife and racing pigeons from Trinidad. J. Wildl. Dis., 34: 73-80.
Bengis, RG; Leighton, FA; Fischer, JR; Artois, M; Morner, T and Tate, CM (2004). The role of wildlife in emerging and re-emerging zoonoses. Rev. Sci. Tech., 23: 497-511.
Broman, T; Waldenstrom, J; Dahlgren, D; Carlsson, I; Eliasson, I and Olsen, B (2004). Diversities and similarities in PFGE profiles of Campylobacter jejuni isolated from migrating birds and humans. J. Appl. Microbiol., 96: 834-843.
Cody, AJ; McCarthy, ND; Bray, JE; Wimalarathna, HM; Colles, FM; Jansen van Rensburg, MJ; Dingle, KE; Waldenstrom, J and Maiden, MC (2015). Wild bird associated Campylobacter jejuni isolates are a consistent source of human disease, in Oxfordshire, United Kingdom. Environ. Microbiol. Rep., 7: 782-788.

Colles, FM; Ali, JS; Sheppard, SK; McCarthy, ND and Maiden, MCJ (2011).Campylobacter populations in wild and domesticated Mallard ducks (Anas platyrhynchos).Environ. Microbiol. Rep., 3: 574-580.

Colles, FM; Dingle, KE; Cody, AJ and Maiden, MC (2008). Comparison of Campylobacter populations in wild geese with those in starlings and free range poultry on the same farm. Appl. Environ. Microbiol., 74: 3583-3590.
Dhama, K; Karthik, K; Chakraborty, S; Tiwari, R and Kapoor, S (2013). Wildlife: a hidden warehouse of zoonosis - a review. Int. J. Curr. Res., 5: 1866-1879.
Haagsma, JA; Siersema, PD; De Wit, NJ and Havelaar, AH (2010). Disease burden of post-infectious irritable bowel syndrome in the Netherlands. Epidemiol. Infect., 138: 1650-1656.
Hald, B; Skov, MN; Nielsen, EM; Rahbek, C; Madsen, JJ; Waino, M; Chriél, M;Nordentoft, S; Baggesen, DL and Madsen, M (2016). Camylobacter jejuni and Campylo-bacter coli in wild birds on Danish livestock farms. Acta Vet. Scand., 58: 11.
Hoar, BM; Whiteside, DP; Ward, L; Inglis, DG and Morck, DW (2007). Evaluation of the enteric microflora of captive whooping cranes (Grus americana) and sandhill cranes (Grus canadensis). Zoo Biol., 26: 141-153.
Hollamby, S; Sikarskie, JG and Stuht, J (2003). Survey of peafowl (Pavo cristatus) for potential pathogens at three Michigan zoos. J. Zoo Wildl. Med., 34: 375-379.
ISO 1072-1:2006. Microbiology of food and animal feeding stuffs- Horizontal method for detection and enumeration of Campylobacter spp. Part 1: Detection method.
Ito, K; Kubokura, Y; Kaneko, K; Totake, Y and Ogawa, M (1988). Occurrence of Campylobacter jejuni in free-living wild birds from Japan. J. Wildl. Dis., 24: 467-470.
Kapperud, G; Espeland, G; Wahl, E; Walde, A; Herikstad, H; Gustavsen, S; Tveit, I; Natas, O; Bevanger, L and Digranes, A (2003). Factors associated with increased and decreased risk of Campylobacter infection: a prospective case-control study in Norway. Am. J. Epidemiol., 158: 234-242.
Kapperud, G and Rosef, O (1983). Avian wildlife reservoir of Campylobacter fetus subsp. jejuni, Yersinia sp., and Salmonella sp. in Norway. Appl. Environ. Microbiol., 45: 375-380.
Keller, JI; Shriver, WG; Waldenström, J; Griekspoor, P and Olsen, B (2011). Prevalence of Campylobacter in wild birds of the Mid-Atlantic region, USA. J. Wildl. Dis., 47: 750-754.
Klena, JD; Parker, CT; Knibb, K; Ibbitt, JC; Devane, PM; Horn, ST and Konkel, ME (2004). Differentiation of
Campylobacter coli
, Campylobacter jejuni, Campylobacter lari, and Campylobacter upsaliensis by a multiplex PCR developed from the nucleotide sequence of the lipid A gene lpxA. J. Clin. Microbiol., 42: 5549-5557.
Koga, T; Aoki, W; Mizuno, T; Wakazono, K; Ohno, J; Nakai, T; Nomiya, T; Fujii, M; Fusegawa, K; Kinoshita, K; Hamada, T and Ikeda, Y (2015). Antimicrobial resistance in Campylobacter coli and Campylobacter jejuni in cynomolgus monkeys (Macaca fascicularis) and eradication regimens. J. Microbiol. Immunol. Infect., 50: 75-82.
Ling, YE; Saleha, AA and Jalila, A (2012). Detection of Campylobacter and Salmonella in ostrich. J. Vet. Malaysia. 24: 6-8.
Linton, D; Lawson, AJ; Owen, RJ and Stanley, J (1997). PCR detection, identification to species level, and fingerprinting of Campylobacter jejuni and Campylobacter coli direct from diarrheic samples. J. Clin. Microbiol., 35: 2568-2572.
Marin, C; Palomeque, M; Marco-Jiménez, F and Vega, S (2014). Wild griffon vultures (Gyps fulvus) as a source of Salmonella and Campylobacter in eastern Spain. PLoS One., 9: e94191.
Mohan, V (2015). Faeco-prevalence of Campylobacter jejuni in urban wild birds and pets in New Zealand. BMC Res. Notes. 8: 1.
Rosef, O; Gondrosen, B; Kapperud, G and Underdal, B (1983). Isolation and characterization of Campylobacter jejuni and Campylobacter coli from domestic and wild animals in Norway. Appl. Environ. Microbiol., 46: 855-859.
Singh, H; Rathore, RS; Singh, S and Cheema, PS (2011). Comparative analysis of cultural isolation and PCR based assay for detection of Campylobacter jejuni in food and faecal samples. Braz. J. Microbiol., 42: 181-186.
Sippy, R; Sandoval-Green, CM; Sahin, O; Plummer, P; Fairbanks, WS; Zhang, Q and Blanchong, JA (2012). Occurrence and molecular analysis of Campylobacter in wildlife on livestock farms. Vet. Microbiol., 157: 369-375.
Stoddard, RA; Gulland, FMD; Atwill, ER; Lawrence, J; Jang, S and Conrad, PA (2005). Salmonella and Campylobacter spp. in northern elephant seals, California. Emerg. Infect. Dis., 11: 1967-1969.
Waldenstrom, J; Broman, T; Carlsson, I; Hasselquist, D; Achterberg, RP; Wagenaar, JA and Olsen, B (2002). Prevalence of Campylobacter jejuni, Campylobacter lari, and Campylobacter coli in different ecological guilds and taxa of migrating birds. Appl. Environ. Microbiol., 68: 5911-5917.
Waldenstrom, J; On, SL; Ottvall, R; Hasselquist, D and Olsen, B (2007). Species diversity of Campylobacteria in a wild bird community in Sweden. J. Appl. Microbiol., 102: 424-432.
Yogasundram, K; Shane, SM and Harrington, KS (1989). Prevalence of Campylobacter jejuni in selected domestic and wild birds in Louisiana. Avian Dis., 33: 664-667.