جداسازی کلستریدیوم دیفیسیل و تشخیص مولکولی توکسین‌های دوگانه A و B در مدفوع سگ‌ها

نوع مقاله : مقاله کوتاه

نویسندگان

چکیده

کلستریدیوم دیفیسیل باکتری گرم مثبت و باسیل تولید کننده توکسینی است که عامل بیماری‌زای مهم روده‌ای در حیوانات و انسان می‌باشد و منجر به مشکلات گوارشی مثل آنتریت، درد شکمی، تب و اسهال می‌شود. توکسین‌های این باکتری شامل انتروتوکسین یا توکسین A و سیتوتوکسین یا توکسین B و توکسین دوگانه می‌باشند. هدف این مطالعه جداسازی باکتری از مدفوع سگ‌ها و مطالعه میزان شیوع ژن‌های حدت در جدایه‌ها بود. در این مطالعه از تعداد 151 نمونه جمع‌آوری گردید. جداسازی باکتری بر اساس کشت به روش استاندارد و استخراج ژنوم با استفاده از روش جوشاندن انجام گرفت. در واکنش زنجیره‌ای پلیمراز چندگانه ژن‌های tcdA، tcdB، cdtA و cdtB و در واکنش تکی پلیمراز tcdC مورد ردیابی قرار گرفت. دوازده نمونه (9/7%) نمونه‌ها در کشت مثبت بوده و بر اساس مطالعه مولکولی 7/66% نمونه‌ها (۸ نمونه کلستریدیوم دیفیسیل از ۱۲ نمونه کلستریدیوم دیفیسیل جدا شده) واجد پروفایل توکسینی tcdA+، tcdB+ بودند. این مطالعه اولین تحقیق در مورد کلستریدیوم دیفیسیل بر روی جمعیت سگ‌ها در ایران می‌باشد.

کلیدواژه‌ها


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