Effects of dietary supplementation of mannan-oligosaccharide on virus shedding in avian influenza (H9N2) challenged broilers

Document Type : Short paper


1 MPhil/Ms in Physiology, Department of Physiology, University of Veterinary and Animal Sciences, Lahore-5400, Punjab, Pakistan

2 MPhil/Ms in Genetic Engineering and Biotechnology, Institute of Biotechnology and Genetic Engineering, The University of Agriculture, Peshawar-25120, KPK, Pakistan

3 MPhil/Ms in Livestock Management, Department of Livestock Management, Faculty of Animal Husbandry and Veterinary Sciences, The University of Agriculture, Peshawar-25120, KPK, Pakistan

4 MPhil/Ms in Biotechnology, Department of Biotechnology, Quaid-i-Azam University, Islamabad, Pakistan

5 Department of Animal Health, Faculty of Animal Husbandry and Veterinary Sciences, The University of Agriculture, Peshawar-25120, KPK, Pakistan


Avian influenza (AI) is a highly contagious disease causing significant economic losses worldwide. The aim of this study is to evaluate the effect of mannan-oligosaccharide (MOS) on tracheal and cloacal virus shedding in AI challenged broilers and contamination of environment with H9N2. A total of 300 1-day-old-broiler chicks were randomly divided into 3 groups (A, B and C) and supplemented 0.2, 0.5 and 0.0% MOS, respectively in NRC recommended diet for 36 days. On day 21 the groups were further split into two sub groups A+ve, A-ve, B+ve, B-ve, C+ve and C-ve with 5 replicates each. The positive groups were shifted to remote sheds and were challenged intranasally with 0.1 ml of reference virus (AIV; Pk-UDL/01/08 H9N2) with EID50 = 10-6.66. Treatment reduces (P<0.05) cloacal virus shedding from day 24 to 26 and 28 to 32. Tracheal virus shedding was lower (P<0.05) on days 25-26 and 28-30 in treatment groups. Day 27 showed highest (P>0.05) virus shedding in all groups. However the reduction of viral shedding is faster in treatment groups and showed no virus shedding on day 32. Maternal antibody titer against AI showed a declining pattern but MOS influenced (P<0.05) the titer in treated groups. Hence the use of MOS may constitute a novel and effective plausible alternative that reduces the spread of disease by decreasing virus shedding and contamination of environment from AIV (H9N2) infection in poultry.


Alexander, DJ and Chettle, NJ (1977). Procedures for the haemagglutination and the haemagglutination inhibition tests for avian infectious bronchitis virus. Avian Pathol., 6: 9-17.
Bhatti, BM (1995). A review of incidence of avian influenza disease. Agro-Livestock News. 1: 1-4.
Cotter, PF; Malzone, A; Paluch, B; Lilburn, MS and Sefton, AE (2000). Modulation of humoral immunity in commercial laying hens by a dietary prebiotic. Poult. Sci., 79(S1): 38 (abst.).
Duncan, DB (1955). Multiple range and multiple F tests. Biometrics. 11: 1-42.
Fang, SB; Lee, HC; Hu, JJ; Hou, SY; Liu, HL and Fang, HW (2009). Dose-dependent effect of Lactobacillus rhamnosus on quantitative reduction of faecal rotavirus shedding in children. J. Trop. Pediatr., 55: 297-301.
Ganguli, K; Meng, D; Rautava, S; Lu, L; Walker, WA and Nanthakumar, N (2013). Probiotics prevent necrotizing enterocolitis by modulating enterocyte genes that regulate innate immune-mediated inflammation. Am. J. Physiol. Gastrointest. Liver Physiol., 304: 132-141.
Glenn, GR and Roberfroid, MB (1995). Dietary modulation of the human colonic microbiota: introducing the concepts of prebiotics. J. Nutr., 125: 1401-1412.
Havenaar, R and Huis In’t Veld, JHJ (1992). Probiotics: A general view in the lactic acid bacteria in health and disease. Vol. 1. WOOD JB.
Janardhana, V; Broadway, MM; Bruce, MP; Lowenthal, JW; Geier, MS; Hughes, RJ and Bean, AG (2009). Prebiotics modulate immune responses in the gut-associated lymphoid tissue of chickens. J. Nutr., 139: 1404-1409.
Kawase, M; He, F; Kubota, A; Harata, G and Hiramatsu, M (2010). Oral administration of lactobacilli from human intestinal tract protects mice against influenza virus infection. Lett. Appl. Microbiol., 51: 6-10.
Lourenço, MC; de Souza, AM; Hayashi, RM; da Silva, AB and Santin, E (2016). Immune response of broiler chickens supplemented with prebiotic from Sacharomyces cerevisiae challenged with Salmonella enteritidis or Minnesota. J. Appl. Poult. Res., 25: 165-172.
National Research Council (2001). Nutrient requirements for poultry. 11th Revised ED. Washington, D.C., USA, NationalAcademy Press.
Noble, GR (1982). Epidemiological and clinical aspects of influenza. In: Beare, AS (Ed.), Basic and applied influenza research. Boca Raton (Florida), CRC Press. PP: 11-50.
Oliveira, MC; Figueiredo-Lima, DF; Faria, DE; Marques, RH and Moraes, VMB (2009). Effect of mannanoligo-saccharides and/or enzymes on antibody titers against infectious bursal and Newcastle disease virus. Arq. Bras. Med. Vet. Zootec., 61: 6-11.
Oyofo, BA; DeLoach, JR; Corrier, DE; Norman, JO; Ziprin, RL and Mollenhauer, HH (1989). Prevention of Salmonella typhimurium colonization of broilers with D-mannose. Poult. Sci., 68: 1357-1360.
Poorbaghi, SL; Dadras, H; Gheisari, HR; Mosleh, N; Firouzi, S and Roohallazadeh, H (2013). Effects of Lactobacillus acidophilus and inulin on faecal viral shedding and immunization against H9N2 avian influenza virus. J. Appl. Microbiol., 116: 667-676.
Qaisrani, SN; van Krimpen, MM; Kwakkel, RP; Verstegen, MWA and Hendriks, WH (2015). Diet structure, butyric acid, and fermentable carbohydrates influence growth performance, gut morphology, and cecal fermentation characteristics in broilers. Poult. Sci., 94: 2152-2164.
Qiao, H; Duffy, LC; Griffiths, E; Dryja, D; Leavens, A; Rossman, JON; Rich, G; Riepenhoff-Talty, M and Locniskar, M (2002). Immune responses in rhesus rotavirus-challenged Balb/c mice treated with Bifido-bacteria and Prebiotic supplements. Pediatr. Res., 51: 750-755.
Raju, MVLN and Devegowda, G (2002). Esterified-glucomannan in broiler chicken diets-contaminated with aflatoxin, ochratoxin and T-2 toxin: Evaluation of its binding ability (in vitro) and efficacy as immunomodulator. Asian Aust. J. Anim. Sci., 15: 1051-1056.
Reed, LJ and Muench, H (1938). A simple method of estimating fifty percent endpoints. Am. J. Epidemiol., 27: 493-497.
Ribeiro, AML; Vogt, LK; Canal, CW; Cardoso, M; Labres, RV; Sreack, AF and Bessa, MC (2007). Effects of prebiotics and probiotics on the colonization and immune response of broiler chickens challenged with Salmonella Enteritidis. Braz. J. Poult. Sci., 9: 193-200.
Rickard, ER; Thigpen, M and Crowley, JH (1944). The isolation of influenza A virus by the intra-allantoic inoculation of chick embryos with untreated throat-washings. J. Immunol., 49: 263-271.
Saf-Agric Inc. (2007). SAF-Mannan Product Information Sheet. www.saf-agri.com/english/inomos.htm., Accessed Aug.17.2015.
Schley, PD and Field, CJ (2002). The immune-enhancing effects of dietary fibres and prebiotics. Brit. J. Nutr., 87(S2): 221-230.
Shahir, MH; Sharifi, M; Afsarian, O and Mousavi, SS (2014). A comparison of the effects of commercial prebiotic (safmannan®, biomos® and fermacto®) on performance, egg quality and antibody titer of avian influenza and Newcastle disease in laying hens. J. Vet.
., 69: 79-84.
Shashidhara, RG and Devegowda, G (2003). Effect of dietary mannan oligosaccharide on broiler breeder production traits and immunity. Poult. Sci., 82: 1319-1325.
Silva, VK; da Silva, JDT; Torres, KAA; de Faria Filho, DE; Hada, FH and de Moraes, VB (2009). Humoral immune response of broilers fed diets containing yeast extract and prebiotics in the prestarter phase and raised at different temperatures. J. Appl. Poult. Res., 18: 530-540.
Spring, P; Wenk, C; Dawson, KA and Newman, KE (2000). The effect of dietary mannaoligosaccharides on cecal parameters and the concentrations of enteric bacteria in the ceca of salmonella-challenged broiler chicks. Poult. Sci., 79: 205-211.
Steel, RGD and Dickey, DAJH (1997). Principles and procedures of statistics: a biometrical approach. McGraw-Hill series in probability and statistics.
Strickling, JA; Harmon, DL; Dawson, KA and Gross, KL (2000). Evaluation of oligosaccharide addition to dog diets: influences on nutrient digestion and microbial populations. Anim. Feed Sci. Technol., 86: 205-219.
Tohid, T; Hasan, G and Alireza T (2010). Efficacy of mannanoligosaccharides and humate on immune response to Avian Influenza (H9) disease vaccination in broiler chickens. Vet. Res. Commun., 34: 709-717.
Yang, Y; Iji, PA; Kocher, A; Mikkelsen, LL and Choct, M (2007). Effects of mannanoligosaccharide on growth performance, the development of gut microflora, and gut function of broiler chickens raised on new litter. J. Appl. Poult. Res., 16: 280-288.
Youn, LH; Lee, YN; Lee, DH; Park, JK; Yuk, SS; Lee, HJ; Yeo, JM; Yang, SY; Lee, JB; Park, SY; Choi, IS and Song, CS (2012). Effect of intranasal administration of Lactobacillus fermentum CJL-112 on horizontal trans-mission of influenza virus in chickens. Poult. Sci., 91: 2517-2522.