Mammary tumor associated Hspb1 mutation and screening of eight cat populations of the world

Document Type : Full paper (Original article)

Authors

1 Department of Biotechnology, Virtual University of Pakistan, Lahore, Pakistan

2 Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore, Pakistan

3 Department of Veterinary Medicine & Surgery, College of Veterinary Medicine, University of Missouri-Columbia, Columbia, MO 65211, USA

Abstract

Current research highlights the Hspb1 based screening of eight cat populations of the world to investigate the association of newly found locus within cat mammary tumors. Total 180 cats were screened on the basis of Hspb1 4 bp deletion locus (1514-1517del4) which was observed in six mammary tumor cases in Siamese cat breed. Case-control association study revealed the non-significance with P=0.201 and an overall mutant allele frequency of 0.30 ranging from 0.20-0.40 was observed in other cat populations. Similarly, HWE was also obeyed in combined population samples with P=0.860 and found non-significant with range of 0.429-0.708 in other non-Pakistani cat populations as well. These results might be helpful to understand the association of this novel locus in a better way with large sample size of cases and may also serve as a potential marker for mammary tumor diagnosis, particularly in cats and generally in all other animal populations in comparative genetics and genomics context.

Keywords

References

Bell, J (2008). A simple way to treat PCR products prior to sequencing using ExoSAP-IT. Biotechniques. 44: 834.
Burrai, GP; Mohammed, SI; Miller, MA; Marras, V; Pirino, S; Addis, MF; Uzzau, S and Antuofermo, E (2010). Spontaneous feline mammary intraepithelial lesions as a model for human estrogen receptor and progesterone receptor-negative breast lesions. BMC Cancer. 10: 156.
Corporation, GC (2010). Sequencher version 5.2. Sequence analysis software. Ann. Arbor Michigan.
Fernández, XM and Birney, E (2010). Ensembl genome browser. In: Michael Speicher, M; Antonarakis, SE and Motulsky, AG (Eds.), Vogel and Motulsky’s human genetics. (4th Edn.), New York, USA, Springer. PP: 923-939.
Garrido, C; Schmitt, E; Candé, C; Vahsen, N; Parcellier, A and Kroemer, G (2003). HSP27 and HSP70: potentially oncogenic apoptosis inhibitors. Cell Cycle. 2: 578-583.
Giménez, F; Hecht, S; Craig, LE and Legendre, AM (2010). Early detection, aggressive therapy optimizing the manage-ment of feline mammary masses. J. Feli. Medi. Surg., 12: 214-224.
Hughes, K and Dobson, J (2012). Prognostic histopatholo-gical and molecular markers in feline mammary neoplasia. Vet. J., 194: 19-26.
Knudson, AG (1971). Mutation and cancer: statistical study of retinoblastoma. Pro. Nati. Acad. Sci., 68: 820-823.
Lee, S; Taylor, J; Innis, M; Gelfand, D; Sninsky, J and White, T (1990). Isolation of DNA from fungal mycelia
and single spores. PCR protocols, aguide to methods and applications. 2nd Edn., Orlando, Florida, Academic Press. PP: 282-287.
Ozcelik, H; Shi, X; Chang, MC; Tram, E; Vlasschaert, M; Di Nicola, N; Kiselova, A; Yee, D; Goldman, A and Dowar, M (2012). Long-range PCR and next-generation sequencing of BRCA1 and BRCA2 in breast cancer. J. Mol. Diagn., 14: 467-475.
Rozen, S and Skaletsky, H (2011). Primer3. 1998. Code available at http://www-genome wi mit edu/genome_ software/other/primer3 html.
Rutteman, G and Misdorp, W (1992). Hormonal background of canine and feline mammary tumours. J. Repr. Fert., Suppl., 47: 483-487.
Sambrook, J and Russell David, W (1989). Molecular cloning: a laboratory manual. 2nd Edn., Vol. 3, New York, USA, ColdSpringHarbor Laboratory Press. PP: 1468-1470.
Shafiee, R; Javanbakht, J; Atyabi, N; Bahrami, A; Kheradmand, D; Safaei, R; Khadivar, F and Hosseini, E (2013). Comparative value of clinical, cytological, and histopathological features in feline mammary gland tumors; an experimental model for the study of human breast cancer. Diagn. Pathol., 8: 1596-1598.
Vogelstein, B and Gillespie, D (1979). Preparative and analytical purification of DNA from agarose. Pro. Nati. Acad. Sci., 76: 615-619.
Zappulli, V; De Zan, G; Cardazzo, B; Bargelloni, L and Castagnaro, M (2005). Feline mammary tumours in comparative oncology. J. Dairy Res., 72: 98-106.
Zauber, AG; Lansdorp-Vogelaar, I; Knudsen, AB; Wilschut, J; Van Ballegooijen, M and Kuntz, KM (2008). Evaluating test strategies for colorectal cancer screening: a decision analysis for the US Preventive Services Task Force. Ann. Inter. Med., 149: 659-669.

Files

Share

How to cite

Statistics