Estrogen receptor gene 1 expression in caprine and its effect on fertility

Document Type : Short paper

Authors

1 Physiology Reproduction and Shelter Management (PRSM) Division, Central Institute for Research on Goats (CIRG), Makhdoom, Farah-281122, Mathura, Uttar Pradesh, India

2 GGB Division, Central Institute for Research on goats (CIRG), Makhdoom, Farah- 281122, Mathura, Uttar Pradesh, India

Abstract

The present study was undertaken to analyze the expression pattern of estrogen receptor 1 gene (ESR1) in Barbari bucks (fertile and non-fertile) identified on the basis of seminal quality traits and fertility trials. RNA was extracted from the spleen by Trizol method. The expression pattern of ESR1 gene was analyzed using real time polymerase chain reaction (RT-PCR). The expression pattern of ESR1 gene was analyzed by RT-PCR (Roche LC-480). Relative quantification by RT-PCR indicated that the ESR1 gene expression showed more fold in fertile bucks as compared to non-fertile.

Keywords

References

Bakker, J and Brock, O (2010). Early oestrogens in shaping reproductive networks: evidence for a potential organisa-tional role of oestradiol in female brain development. J. Neuroendocrinol., 22: 728-735.
Carreau, S; Bourguiba, S; Lambard, S; Galeraud-Denis, I; Genissel, C and Levallet, J (2002). Reproductive system: aromatase and estrogens. Mol. Cel. Endocrinol., 193: 137-143.
Fibbi, B; Filippi, S; Morelli, A; Vignozzi, L; Silvestrini, E and Chavalmane, A (2009). Estrogens regulate humans and rabbit epididymal contractility through the RhoA/Rho-kinase pathway. J. Sex. Med., 6: 2173-2186.
Filippi, S; Morelli, A; Vignozzi, L; Vannelli, GB; Marini, M and Ferruzzi, P (2005). Oxytocin mediates the estrogen-dependent contractile activity of endothelin-1 in human and rabbit epididymis. Endocrinology. 146: 3506-3517.
Flouriot, G; Brand, H; Denger, S; Metivier, R; Kos, M; Reis, G; Sonntag-Buck, V and Gannon, F (2000). Identification of a new isoform of the human estrogen receptor-alpha that is encoded by distinct transcripts and that is able to repress activation function-1. EMBO J., 19: 4688-4700.
Fu, M; Xiong, XR; Lan, DL and Li, J (2014). Molecular characterization and tissue distribution of estrogen receptor genes in domestic yak. Asian Australian J. Anim. Sci., 27: 1684-1690.
Hewitt, SC and Korach, KS (2003). Oestrogen receptor knockout mice: roles for oestrogen receptors alpha and beta in reproductive tissues. Reproduction. 125: 143-149.
Irsik, DL; Carmines, PK and Lane, PH (2013). Classical estrogen receptors and era splice variants in the mouse. PLoS One. 8: e70926.
Katsu, Y; Taniguchi, E; Urushitani, H; Miyagawa, S; Takase, M; Kubokawa, K; Tooi, O; Oka, T; Santo, N; Myburgh, J; Matsuno, A and Lguchi, T (2010). Molecular cloning and characterization of ligand- and species-specificity of amphibian estrogen receptors. Gen. Comp. Endocrinol., 168: 220-230.
Massart, S; Milla, S and Kestemont, P (2014). Expression of gene, protein and immunohistochemical localization of the estrogen receptor isoform ERα1 in male rainbow trout lymphoid organs: indication of the role of estrogens in the regulation of immune mechanisms. Comp. Biochem. Physiol., 174: 53-61.
McCarthy, M (2010). How it’s made: organisational effects of hormones on the developing brain. J. Neuroendocrinol., 22: 736-742.
Vasudevan, N and Pfaff, DW (2008). Non-genomic actions of estrogens and their interaction with genomic actions in the brain. Front. Neuroendocrinol., 29: 238-257.
Zhou, D; Li, S; Wang, X; Cheng, B and Ding, X (2011). Estrogen receptor alpha is essential for the proliferation of prostatic smooth muscle cells stimulated by 17betaestradiol and insulin-like growth factor 1. Cell. Biochem. Funct., 29: 120-125.

Files

Share

How to cite

Statistics