Evaluating a murine model of endometritis using uterine isolates of Escherichia coli from postpartum buffalo

Document Type : Full paper (Original article)

Authors

1 Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India

2 Division of Biological Standardization, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India

3 Divsion of Pathology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India

4 Division of Temperate Animal Husbandry, Indian Veterinary Research Institute, Mukteswar, Uttarakhand, 263138, India

5 Division of Livestock Production and Management, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India

6 Ph.D. Scholar, Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India

7 Immunology Section, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India

Abstract

Ascending infection of the uterus with Gram-negative bacteria is responsible for postpartum endometritis in cattle and buffalo and can adversely affect fertility. Development of a laboratory animal model for bovine endometritis would facilitate the understanding of the pathogenesis as it is difficult to conduct controlled experimentation in the native host. In the present study, 30 virgin Swiss Albino mice (5-8 weeks old) were used to evaluate the pathogenic potential of Escherichia coli, isolated from the normally calved postpartum buffalo to induce endometritis. Mice in the diestrus phase of the estrous cycle were randomly allotted to
one of the following four intravaginal inoculation (100 μL) treatments: EG (experimental group)-1: sterile normal saline; EG-2, -3 and -4: E. coli@ 1.5 × 104, 105 and 106 CFU/ml, respectively. The animals were then scarified 36 h post-inoculation to study gross and microscopical lesions. Gross changes were confined to EG-4. Acute endometritis was recorded in 50% of the EG-3 and 66.7% of the EG-4. The rate of acute endometritis development was significantly higher in EG-4 (P<0.05) as compared to the other groups. The present study demonstrated that the animal model for bubaline endometritis can be developed in mice by intravaginal inoculation of E. coli@ 1.5 × 106 CFU/ml at diestrus. Ease of intravaginal inoculation, apparent absence of systemic involvement and high infective rate are the advantages of the model over other studies.

Keywords

References

Agnew, DW; Corbeil, LB; Munson, L; Byrne, BA and Bondurant, RH (2008). A pregnant mouse model for bovine Tri Trichomonas foetus infection. Vet. Pathol., 45: 849-864.
Aisemberg, J; Vercelli, C; Billi, S; Ribeiro, ML; Ogando, D; Meiss, R; McCann, SM; Rettori, V and Franchi, AM (2007). Nitric oxide mediates prostaglandins’ deleterious effect on lipopolysaccharide-triggered murine fetal resorp-tion. Proc. Natl. Acad. Sci. U. S. A., 104: 7534-7539.
Azawi, OI (2010). Uterine infection in buffalo cows: a review. Buffalo Bull., 29: 154-171.
Baldwin, CL and Parent, M (2002). Fundamentals of host immune response against Brucella abortus: what the mouse model has revealed about control of infection. Vet. Microbiol., 90: 367-382.
Bernstein-Hanley, I; Coers, J; Balsara, ZR; Taylor, GA; Starnbach, MN and Dietrich, WF (2006). The p47 GTPases Igtp and Irgb10 map to the Chlamydia trachomatis susceptibility locus Ctrq-3 and mediate cellular resistance in mice. Proc. Natl. Acad. Sci. U. S. A., 103: 14092-14097.
Bicalho, MLS; Machado, VS; Oikonomou, G; Gilbert, RO and Bicalho, RC (2012). Association between virulence factors of Escherichia coli, Fusobacterium necrophorum, and Arcanobacterium pyogenes and uterine diseases of dairy cows. Vet. Microbiol., 157: 125-131.
Byers, SL; Wiles, MV; Dunn, SL and Taft, RA (2012). Mouse estrous cycle identification tool and images. PLoS One. 7: e35538.
Corbeil, LB (1980). Criteria for development of animal models of diseases of the reproductive system. Am. J. Pathol., 101: S241.
Deb, K; Chaturvedi, MM and Jaiswal, YK (2005). Gram-negative bacterial LPS induced poor uterine receptivity and implantation failure in mouse: alterations in IL-1beta expression in the preimplantation embryo and uterine horns. Infect. Dis. Obstet. Gynecol., 13: 125-133.
Dohmen, MJ; Joop, K; Sturk, A; Bols, PE and Lohuis, JA (2000). Relationship between intra-uterine bacterial contamination, endotoxin levels and the development of endometritis in postpartum cows with dystocia or retained placenta. Theriogenology. 54: 1019-1032.
Dombroski, RA; Woodard, DS; Harper, MJK and Gibbs, RS (1990). A rabbit model for bacteria-induced preterm pregnancy loss. Am. J. Obstet. Gynecol., 163: 1938-1943.
Gravett, MG; Witkin, SS; Haluska, GJ and Edwards, JL (1994). An experimental model for intraamniotic infection and preterm labor in rhesus monkeys. Am. J. Obstet. Gynecol., 171: 1660-1667.
Hao, Y; Wang, C; Zhao, B; Qi, X and Zhao, X (2010). Establishment and dynamic histopathology observation of rats model with endometritis. J. Northwest. A&FUniv., 38: 1-6.
Hasan, HF; Hamzah, AM and Zghair, ZR (2013). Study the comparative effect between Cyperus esculentus seeds extract and gentamicin on induced endometritis in mice. JPCS., 7: 40-47.
Hawk, HW; Brinsfield, TH; Turner, GD; Whitmore, GW and Norcross, MA (1964). Effect of ovarian status on acute inflammatory responses in cattle uteri. Am. J. Vet. Res., 25: 362-366.
Hawk, HW; Turner, GD and Sykes, JF (1960). The effect of ovarian hormones on the uterine defence mechanism during the early stage of induced infection. Am. J. Vet. Res., 21: 644-648.
Hawk, HW; Turner, GD and Sykes, JF (1961). Variation in the inflammatory response and bactericidal activity of the sheep uterus during the estrous cycle. Am. J. Vet. Res., 22: 689-692.
Kita, E; Hiroshi, M and Shuzo, K (1981). A mouse model for the study of gonococcal genital infection. J. Infect. Dis., 143: 67-70.
Lane, MC; Alteri, CJ; Smith, SN and Mobley, HL (2007). Expression of flagella is coincident with uropathogenic Escherichia coli ascension to the upper urinary tract. Proc. Natl. Acad. Sci. U. S. A., 104: 16669-16674.
McLean, AC; Valenzuela, N; Fai, S and Bennett, SA (2012). Performing vaginal lavage, crystal violet staining and vaginal cytological evaluation for mouse estrous cycle staging identification. J. Vis. Exp., 67: e4389-e4389.
Monteavaro, CE; Soto, P; Gimeno, EJ; Echevarría, HM; Catena, M; Portiansky, EL and Barbeito, CG (2008). Histological and lectin binding changes in the genital tract of mice infected with Tritrichomonas foetus. J. Comparative Pathol., 138: 40-45.
Nishikawa, Y and Baba, T (1985). Effects of ovarian hormones on manifestation of purulent endometritis in rat uteruses infected with Escherichia coli. Infect. Immun., 47: 311-317.
Pei, Z and Blaser, MJ (1990). Pathogenesis of Campylobacter
foetus infections: role of surface array proteins in virulence in a mouse model. J. Clin. Invest., 85: 1036.
Reznikov, LL; Fantuzzi, G; Selzman, CH; Shames, BD; Barton, HA; Bell, H; McGregor, JA and Dinarello, CA (1990). Utilization of endoscopic inoculation in a mouse model of intrauterine infection-induced preterm birth: role of interleukin 1β1. Biol. Reprod., 60: 1231-1238.
Runciman, DJ; Anderson, GA and Malmo, J (2009). Comparison of two methods of detecting purulent vaginal discharge in postpartum dairy cows and effect of intrauterine cephapirin on reproductive performance. Aust. Vet. J., 87: 369-378.
Sheldon, IM; Cronin, J; Goetze, L; Donofrio, G and Schuberth, HJ (2009). Defining postpartum uterine disease and the mechanisms of infection and immunity in the female reproductive tract in cattle. Biol. Reprod., 81: 1025-1032.
Sheldon, IM; Noakes, DE; Rycroft, AN; Pfeiffer, DU and Dobson, H (2002). Influence of uterine bacterial con-tamination after parturition on ovarian dominant follicle selection and follicle growth and function in cattle. Reproduction. 123: 837-845.
Sheldon, IM; Rycroft, AN; Dogan, B; Craven, M; Bromfield, JJ; Chandler, A; Roberts, MH; Price, SB; Gilbert, RO and Simpson, KW (2010). Specific strains of Escherichia coli are pathogenic for the endometrium of cattle and cause pelvic inflammatory disease in cattle and mice. PLoS One. 5: e9192.
Torres, AG; Zhou, X and Kaper, JB (2005). Adherence of diarrheagenic Escherichia coli strains to epithelial cells. Infect. Immun., 73: 18-29.
Van Andel, A; Franklin, CL; St Claire, MC; Riley, LK; Besch-Williford, CL and Hook, RR (1996). Lesions of experimental genital Tritrichomonas foetus infections in estrogenized BALB/c mice. Vet. Pathol. Online. 33: 407-411.
Williams, EJ; Fischer, DP; Noakes, DE; England, GC; Rycroft, A; Dobson, H and Sheldon, IM (2007). The relationship between uterine pathogen growth density and ovarian function in the postpartum dairy cow. Theriogenology. 68: 549-559.
Wolf, MK (1997). Occurrence, distribution, and associations of O and H serogroups, colonization factor antigens, and toxins of enterotoxigenic Escherichia coli. Clin. Microbiol. Rev., 10: 569-584.
Zhao, H; Jian, Z; Yao, G; Ma, S; Huang, Y; Lu, X and Wang, M (2008). A discussion of experimental endo-metritis model in rat. J. Xinjiang. Agri. Univ., 3: 002.

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