Herein, we described the first case of self-inoculation in a veterinarian with bovine Johne’s disease
(BJD) attenuated vaccine in Iran that required medical attention. A needle-stick injury to the right thumb of a
young veterinarian during vaccination of cattle with BJD vaccine resulted in an inflammation that not only
failed to resolve but also progressed to a lesion 2.5 cm in diameter. The usual conservative treatment of this
wound was not effective. Surgical intervention for debridement of the inflammatory tissue was not
performed. For diagnosis of lesion, needle biopsy was prepared from the inflamed tissue mass.
Histopathologic examination revealed a tuberculoid granulomatous inflammation without any caseous
necrosis. The wound healed by taking rifampin.