Histological and histomorphometrical changes of different regions of oviduct during follicular and luteal phases of estrus cycle in adult Azarbaijan buffalo

Document Type : Full paper (Original article)


1 Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

2 Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

3 Graduated from Faculty of Veterinary Medicine, Urmia University, Urmia, Iran


In this study the reproductive organs of adult and apparently healthy female Azarbaijan buffaloes were
collected after slaughter from abattoir. Through observation of the ovaries, the luteal and follicular phases of
each buffalo were specified. A total number of 36 oviducts at follicular phase and 36 oviducts at luteal phase
were collected and 3 tissue samples were taken from 3 regions of infundibulum, ampulla and isthmus of each oviduct. Sections were stained through the use of H&E, PAS, verhoffe and toluidine blue methods.
Histological observations revealed that the oviduct consists of 4 layers of mucosa, submucosa, tunica
muscularis and serosa. The primary and secondary folds decreased both in number and in height from
infundibulum to isthmus. Epithelium of folds was composed of simple columnar, although seems
pseudostratified in some areas, and contains ciliated and secretory cells. Histomorphometric examinations of three regions demonstrated that the mean height of primary folds increase and the mean thickness of tunica muscularis decrease at follicular phase. The mean thickness of mucosa-submucosa at follicular phase was slightly similar to luteal phase. More visibility of the ciliated cells and mucosal folds in infundibulum and the increase of their height at follicular phase facilitate the capture of the oocyte; the thick tunica muscularis in isthmus transports sperm cells up; and both require promoting fertilization to occur in ampulla.