The role of the histaminergic system on the inhibitory effect of ghrelin on feed intake in broiler chickens

Document Type : Full paper (Original article)


1 Department of Pathobiology, School of Veterinary Medicine, Lorestan University, Khorramabad, Iran

2 Department of Animal Sciences, Faculty of Agriculture, Lorestan University, Khorramabad, Iran

3 Department of Physiology, College of Veterinary Medicine, Ilam University, Ilam, Iran


This study was conducted to investigate the possible involvement of the histaminergic system in the
mediation of ghrelin-induced feeding behavior in broiler chickens. In the trial 1, the effect of
intracerebroventricular (ICV) injection of ghrelin on feed intake was examined in 3-h feed-deprived broiler
chickens. In the trials 2 and 3, the chickens were received ICV chlorpheniramine (an H1 receptor antagonist)
and cimetidine (an H2 receptor antagonist) prior to ghrelin injection, respectively. Cumulative feed intake
was measured at 3-h post injections. Infusion of ghrelin (0.3, 0.6 and 1.2 nmol) decreased feed intake dosedependently (P<0.05). Pre-treatment of chlorpheniramine (100 μg) attenuated the inhibitory effect of ghrelin on feed intake (P<0.05), while such an effect was not noticed by pre-injection of cimetidine (100 μg). The results suggest that there is an interaction between ghrelin and the histaminergic system (through H1
receptors) on control of feed intake in broiler chickens.