Document Type : Short paper
Authors
1
Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt
2
Department of Theriogenology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt
3
Laboratory of Animal Reproduction, Graduate School of Biosphere Science, Hiroshima University, 1-4-4 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8528, Japan
Abstract
This study was conducted to reveal the estrogenic effects of bisphenol A and o, p’-DDT on quail
embryos. Thirteen fertilized eggs were used as control (injected with 20 μl corn oil), 15 eggs were injected
with estradiol 17β (0.04 mg dissolved in 20 μl corn oil), 20 eggs were injected with BPA (2 mg dissolved in
20 μl corn oil) and 20 eggs were injected with o, p’-DDT (2 mg dissolved in 20 μl corn oil) at day 13 of
incubation. Two days later the livers of the embryos were collected. The DNA was extracted from the liver
for molecular sexing, while total RNA was extracted for vitellogenin II (VTGII) mRNA expression in
embryos. In female embryos, BPA and o, p’-DDT induced variable levels of VTGII mRNA expression,
while in male embryos, o, p’-DDT induced a slightly VTGII mRNA expression. In contrast, there was no
expression of VTGII after BPA injection. In conclusion, the estrogenicity of BPA was lower than o, p’-DDT
and both of them were lower than the estradiol 17β.
Keywords
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