Sister chromatid exchange analysis in some Holstein bulls

Document Type : Scientific report


Department of FMD, Razi Vaccine and Serum Research Institute, Karaj, Iran


Chromosomal appearance of 12 Holstein bulls selected for artificial inseminations were examined by
sister chromatid exchanges (SCEs). For differential staining of sister chromatids bromodeoxyuridine (BrdU)
was inoculated in lymphocyte cultures. The mean, maximum and minimum number of SCE per cell were
determined 6.8 ± 1.14, 8.3 ± 1.1 and 5.7 ± 1.5, respectively. SCE frequencies of all animals were in the
normal level. Also, evaluation of different breeding condition and age range did not show any significant
statistical (P>0.05) effect on SCEs rates