TY - JOUR ID - 5949 TI - Development of a novel and specialized cultivation method for isolating Helicobacter pullorum from chicken meat JO - Iranian Journal of Veterinary Research JA - IJVR LA - en SN - 1728-1997 AU - Akhlaghi, H. AU - Emadi Chashmi, S. H. AU - Jebelli Javan, A. AD - DVM Student, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran AD - Department of Clinical Science, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran AD - Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran Y1 - 2021 PY - 2021 VL - 22 IS - 1 SP - 76 EP - 80 KW - Culture method KW - Helicobacter pullorum KW - PCR KW - Sensitivity KW - Specificity DO - 10.22099/ijvr.2020.38193.5560 N2 - Background: It has become established that Helicobacter pullorum could be isolated from raw chicken meat. Aims: This study was aimed to develop a novel culture method (protocol B) to isolate H. pullorum from chicken meat by adding some modifications to the traditional culture method (protocol A), and as a consequence to compare their sensitivity, specificity, and the accuracy of these methods with polymerase chain reaction (PCR) test. Methods: 400 chicken meat samples were collected from various retail markets and supermarkets. Each sample was processed by protocol A, protocol B, and PCR test. Results: Out of 400 samples, 77 (19.25%), and 163 (40.75%) were culture-positive by protocol A and protocol B, respectively. Using PCR test as a gold standard, 196 (49%) samples were identified as H. pullorum. The specificity for both protocols was determined 100%, while the sensitivity of protocol B and protocol A was assessed 83% and 39%, respectively. Also, the higher and lower accuracy belonged to protocol B (92%) and protocol A (70%), respectively. Conclusion: The methodology designed herein can provide a suitable, approximately sensitive, specific, and accurate method to cultivate H. pullorum from chicken meat. UR - https://ijvr.shirazu.ac.ir/article_5949.html L1 - https://ijvr.shirazu.ac.ir/article_5949_74e3f13b5a442469b39171dd2a77df79.pdf ER -