TY - JOUR ID - 5053 TI - Pendrin and sodium/iodide symporter protein expression in the testicular tissue of normal and diabetic rats in prepubertal and post pubertal stages JO - Iranian Journal of Veterinary Research JA - IJVR LA - en SN - 1728-1997 AU - Karaca, T. AU - Demirtas, S. AU - Uzun Goren, D. AD - Department of Histology and Embryology, Faculty of Medicine, Trakya University, Balkan Campus, 22030, Edirne, Turkey Y1 - 2018 PY - 2018 VL - 19 IS - 4 SP - 255 EP - 261 KW - Diabetes KW - Pendrin KW - Rat KW - Sodium/iodide symporter KW - Testis DO - 10.22099/ijvr.2018.5053 N2 - Pendrin (PDS) and sodium/iodide symporter (NIS) are transmembrane proteins that are located in numerous tissue types, particularly thyroid follicular epithelial cells, where they are entrusted with the regulation of iodine molecules. In the present study, we aimed to clarify changes in PDS and NIS protein expression, in the testicular tissue of prepubertal and post pubertal rats at normal or diabetic conditions. Forty Wistar albino male rats (20 prepubertal and 20 post pubertal) were divided into four groups, as follows: group I was prepubertal control, group II was prepubertal diabetic (60 mg/kg intraperitoneal [ip] streptozotocin [STZ]), group III was post pubertal control, and group IV was post pubertal diabetic (60 mg/kg ip STZ). Ki67 immunoreactivity decreased in testicular tissue of both the prepubertal and post pubertal diabetic groups; the apoptotic tubule index and apoptotic cell number increased in the diabetic groups as compared to the control groups. Pendrin immunoreactivity was detected in seminiferous tubules and Leydig cells; and was significantly reduced in the diabetic groups (P<0.05). The number of cells positive for NIS was significantly decreased in prepubertal and post pubertal rats with diabetes, compared to the controls. Enzyme-linked immunosorbent assay (ELISA) analysis showed that PDS and NIS values were significantly reduced in the prepubertal and post pubertal diabetic groups as compared to the control groups. Our results indicate a potential relationship between puberty and PDS and NIS expression in rat testicular tissue and showed the decreasing effects of diabetes on PDS and NIS expression in testicular tissues in rats. UR - https://ijvr.shirazu.ac.ir/article_5053.html L1 - https://ijvr.shirazu.ac.ir/article_5053_86c78e361019feb4bf0e959d12f1e163.pdf ER -