TY - JOUR ID - 4099 TI - LAMP assay for rapid diagnosis of cow DNA in goat milk and meat samples JO - Iranian Journal of Veterinary Research JA - IJVR LA - en SN - 1728-1997 AU - Deb, R. AU - Sengar, G. S. AU - Singh, U. AU - Kumar, S. AU - Raja, T. V. AU - Alex, R. AU - Alyethodi, R. R. AU - Prakash, B. AD - Molecular Genetics Laboratory, ICAR-Central Institute for Research on Cattle, Meerut-250 001, Uttar Pradesh, India AD - MSc in Biotechnology, Molecular Genetics Laboratory, ICAR-Central Institute for Research on Cattle, Meerut-250 001, Uttar Pradesh, India Y1 - 2017 PY - 2017 VL - 18 IS - 2 SP - 134 EP - 137 KW - Cow KW - Goat KW - LAMP assay KW - Meat KW - Milk DO - 10.22099/ijvr.2017.4099 N2 - Animal species detection is one of the crucial steps for consumer’s food analysis. In the present study we developed an in-house built loop-mediated isothermal amplification (LAMP) assay for rapid detection of adulterated cow DNA in goat milk/meat samples. The cow milk/tissue DNA in goat milk/meat samples were identified in the developed LAMP assay by either naked eye visualizing with SYBR Green I dyes or by detecting the typical ladder pattern on gel electrophoresis. This test can detect up to minimum 5% level of cow components admixed in goat milk/meat samples and can be completed within 1 h 40 min starting from DNA extraction from milk/meat samples and can be performed in a water bath. Developed LAMP methodology is simple; rapid and sensitive techniques that can detect adulterant like cow components in goat milk/meat are more accurate than other existing DNA based technologies. UR - https://ijvr.shirazu.ac.ir/article_4099.html L1 - https://ijvr.shirazu.ac.ir/article_4099_33749f0271c58f2cf4d9b5c9da45608d.pdf ER -