TY - JOUR ID - 4095 TI - Transfection of bovine spermatogonial stem cells in vitro JO - Iranian Journal of Veterinary Research JA - IJVR LA - en SN - 1728-1997 AU - Tajik, P. AU - Hoseini Pajooh, Kh. AU - Fazle Elahi, Z. AU - Javdani Shahedin, G. AU - Ghasemzadeh-Nava, H. AD - Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran AD - Department of Biotechnology, Iranian Research Organization for Science and Technology, Tehran, Iran AD - Post-Graduates, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran AD - Department of Clinical Science, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Y1 - 2017 PY - 2017 VL - 18 IS - 2 SP - 113 EP - 118 KW - EGFP KW - Lipofection KW - Spermatogonial stem cells DO - 10.22099/ijvr.2017.4095 N2 - Spermatogonial stem cells (SSCs) are the only stem cells in adults that can transfer genetic information to the future generations. Considering the fact that a single SSC gives rise to a vast number of spermatozoa, genetic manipulation of these cells is a potential novel technology with feasible application to various animal species. The aim of this study was to evaluate enhanced green fluorescent protein (EGFP) gene transfection into bovine SSCs via liposome carrier and assess the best incubation day in uptake exogenous gene by SSCs. Transfection efficiency of EGFP gene with lipofectamine 2000 was determined in days following each three day of transfection (day 4, 6 and 8 of the culture) by fluorescent microscope. Results showed that the transfected cells through lipofection increased significantly (P<0.05) in each three days of transfection in comparison with those of the control groups. The transfected SSCs were higher in comparison with those of the free exogenous gene carrier groups (P<0.05). In comparison with these three days, the rate of infected cells was higher when transfection proceeds at day four. It was concluded that lipofectamine can be used safely for direct loading exogenous DNA to SSCs particularly during the fourth day of culture. UR - https://ijvr.shirazu.ac.ir/article_4095.html L1 - https://ijvr.shirazu.ac.ir/article_4095_209a0c25c3fc069a9eb91415d912dc32.pdf ER -