TY - JOUR ID - 3035 TI - Effect of thermal stress on expression profile of apoptosis related genes in peripheral blood mononuclear cells of transition Sahiwal cow JO - Iranian Journal of Veterinary Research JA - IJVR LA - en SN - 1728-1997 AU - Somal, A. AU - Aggarwal, A. AU - Upadhyay, R.C. AD - Ph.D. Scholar in Veterinary Physiology, Physiology and Climatology (P&C) Division, Indian Veterinary Research Institute, Izatnagar, 243122, Bareilly, Uttar Pradesh, India AD - Dairy Cattle Physiology Division, National Dairy Research Institute, Karnal-132001, Haryana, India Y1 - 2015 PY - 2015 VL - 16 IS - 2 SP - 137 EP - 143 KW - Apoptosis KW - PBMC KW - Sahiwal KW - Thermal stress KW - Transition cattle DO - 10.22099/ijvr.2015.3035 N2 - The study was conducted to evaluate the effect of thermal stress on expression profile of genes related to apoptosis in peripartum Sahiwal cows. For this, twelve pregnant dry Sahiwal cows were selected from Livestock Research Centre at National Dairy Research Institute, Karnal. The cows were divided into two groups consisting of six Sahiwal cows each. Cows of group I calved during thermoneutral temperature conditions (THI=67.3) and cows of group II calved in summer season (THI=79.9). Blood sampleswere collected on -15, 0 and +15 days with respect to calving where day ‘0’ represents the day of calving. The peripheral blood mononuclear cells (PBMC) were separated and total RNA was isolated for the BCL-2 (B-Cell Lymphoma-2), BAX (BCL-2 antagonist killer-1), BAK (Bcl-2-associated X protein), CASP-3 (cysteine-aspartic proteases-3) and P53 (tumour protien-53) mRNAs expression. It was found that there was up regulation of CASP-3 on the day of calving during both temperature conditions. Comparison between the two temperature conditions showed that expression of CASP-3, BCL-2, BAK, P53 and ratio of BAX/BCL-2 in PBMC increased during summer as compared to thermoneutral condition suggesting the susceptibility of these cells to apoptosis. Based on the above findings it can be concluded that during calving PBMC are more susceptible to apoptosis, and summer being more stressful potentiates the apoptosis of PBMC in Sahiwal cows. UR - https://ijvr.shirazu.ac.ir/article_3035.html L1 - https://ijvr.shirazu.ac.ir/article_3035_8c351103114ba6b2a33ba2052bd62ad2.pdf ER -