TY - JOUR ID - 2931 TI - Clinico-pathological findings of Clostridium perfringens type D enterotoxaemia in goats and its hemolytic activity in different erythrocytes JO - Iranian Journal of Veterinary Research JA - IJVR LA - en SN - 1728-1997 AU - Ali Nasir, A. AU - Younus, Younus, M. AU - Rashid, A. AU - Abdul Khaliq, S. AU - Khan, E. AU - Shah, S. H. AU - Aslam, A. AU - Ghumman, M. A. AU - Joiya, M. H. AD - Division of Bacteriology, Veterinary Research Institute, Lahore Cantt-54810, Pakistan AD - Department of Pathology, College of Veterinary Science, Jhang Campus, Jhang, Pakistan AD - Department of Media Sterilization, University of Veterinary and Animal Sciences, Lahore, Pakistan Y1 - 2015 PY - 2015 VL - 16 IS - 1 SP - 94 EP - 99 KW - Clostridium perfringens type D KW - Enterotoxaemia ELISA KW - Alpha and Epsilon genes KW - Hemolysis DO - 10.22099/ijvr.2015.2931 N2 - The present investigation was conducted to study the effects of experimental Clostridium perfringens type D enterotoxaemia in teddy goats. Clinical signs started to appear after 30 min of experimental infection like anorexia, diarrhea, dehydration, frothing and dyspnea. Gross lesions consisted of severe congestion in tissues of varying intensity with enlarged mesenteric lymph nodes while histological examination revealed edema of lungs, kidney, and lymph nodes and to some extent in brain along with hemorrhages inlungs and intestines. Clostridium perfringens type D carrying alpha and epsilon toxin genes were amplified with amplicon size about 247 bp and 665 bp, respectively. Human erythrocytes showed the highest hemolysis, 68%, followed by mice, 57%, against culture supernatants. The percentage of hemolysis was significantly higher at 37°C as compared to 25°C except for rabbit and dog. UR - https://ijvr.shirazu.ac.ir/article_2931.html L1 - https://ijvr.shirazu.ac.ir/article_2931_4185c2d98e5a1653c620cd97ecbc5355.pdf ER -