TY - JOUR ID - 2922 TI - Effect of different activators on development of activated in vitro matured caprine oocytes JO - Iranian Journal of Veterinary Research JA - IJVR LA - en SN - 1728-1997 AU - Sharma, J. R. AU - Agarwal, S. AU - Kharche, S. D. AU - Goel, A. K. AU - Jindal, S. K. AU - Agarwal, S. K. AD - MSc, Physiology Reproduction and Shelter Management Division, Central Institute for Research on Goats (CIRG), Makhdoom, Farah-281122, Mathura, Uttar Pradesh, India AD - Ph.D. Scholar, Physiology Reproduction and Shelter Management Division, Central Institute for Research on Goats (CIRG), Makhdoom, Farah-281122, Mathura, Uttar Pradesh, India AD - Physiology Reproduction and Shelter Management Division, Central Institute for Research on Goats (CIRG), Makhdoom, Farah-281122, Mathura, Uttar Pradesh, India AD - Physiology Reproduction and Shelter Management Division, Central Institute for Research on Goats (CIRG), Makhdoom, Farah-281122, Mathura, Uttar Pradesh, India Y1 - 2015 PY - 2015 VL - 16 IS - 1 SP - 42 EP - 46 KW - Parthenogenesis KW - Ethanol activation KW - Morula KW - Blastocyst KW - Caprine DO - 10.22099/ijvr.2015.2922 N2 - This study was designed to compare the effectiveness of different activation treatments for activation of in vitro matured oocytes and their developmental potency in mCR2aa medium so as to obtain maximum number of embryos. A total of 1090 cumulus oocyte complexes (COC’s) were collected from 480 ovaries. In vitro matured oocytes were randomly divided into four groups. Group 1 in vitro matured oocytes (n=226) were exposed to 7% ethanol for 5 min followed by treatment with 2.0 mM DMAP for 4 h in mCR2aamedium. Group 2 in vitro matured oocytes (n=294) were exposed to 7% ethanol for 5 min followed by treatment with 10 μg/ml CHX for 4 h in mCR2aa medium. Group 3 in vitro matured oocytes (n=325) were exposed to 7% ethanol for 5 min followed by treatment with 2.0 mM DMAP and 10 μg/ml CHX for 4 h in mCR2aa medium. Group 4 in vitro matured oocytes (n=108) were cultured for 4 h without any chemical treatment in mCR2aa medium (control). The cleavage rate in groups 1, 2, 3 and 4 was 54.42%, 44.55%,51.69% and 0.00%, respectively. The percentage of morula and blastocyst production in group 1, group 2 and group 3 was 26.01%, 29.77% and 29.76% and 2.43%, 1.52% and 1.78%, respectively. These results suggest that the activation of in vitro matured oocytes by 7% ethanol for 5 min followed by treatment with 2.0 mM DMAP for 4 h in mCR2aa is most favorable for parthenogenetic caprine embryos production. UR - https://ijvr.shirazu.ac.ir/article_2922.html L1 - https://ijvr.shirazu.ac.ir/article_2922_3e70e9d7b23437b1e8f894d6bd3f6a8d.pdf ER -