TY - JOUR ID - 1689 TI - SDS-PAGE analysis of urinary proteins in dogs with heartworm disease JO - Iranian Journal of Veterinary Research JA - IJVR LA - en SN - 1728-1997 AU - Beristain-Ruiz, D.M. AU - Carretón, E. AU - Rodríguez-Alarcón, C.A. AU - Montoya-Alonso, J.A. AU - Barrera, R. AD - Department of Veterinary Science, Institute of Biological Sciences, Autonomous University of Juarez, Ciudad Juárez, México AD - Department of Internal Medicine, Faculty of Veterinary Medicine, University of Las Palmas de Gran Canaria, Las Palmas, Spain AD - Department of Animal Medicine, Faculty of Veterinary Sciences, University of Extremadura, Cáceres, Spain Y1 - 2013 PY - 2013 VL - 14 IS - 3 SP - 245 EP - 249 KW - Dirofilaria immitis KW - SDS-PAGE KW - Proteinuria DO - 10.22099/ijvr.2013.1689 N2 - The aim of the study was to describe the urinary electrophoretic pattern of dogs with heartworm disease.Urine samples from 15 heartworm-infected and 15 healthy dogs were taken. Urinary specific gravity, urinaryprotein concentration and the urine protein/creatinine (U P/C) ratio were determined. Urine proteins werefractionated using SDS-PAGE. Results showed statistically significant differences for the U P/C ratio(P<0.05) but not for USG and urinary protein concentrations (P>0.05) between groups. Urinary protein SDSPAGE analysis showed eight distinct bands in the urine of heartworm-infected dogs. The presence of proteins exclusively found in the urine of infected dogs suggests renal damage, even in cases of light proteinuria, indicating that SDS-PAGE is a sensitive method for the identification and characterisation of renalproteinuria in dogs with heartworm disease. UR - https://ijvr.shirazu.ac.ir/article_1689.html L1 - https://ijvr.shirazu.ac.ir/article_1689_9820b72c71f16007236a1cb2b7aa3e5d.pdf ER -