TY - JOUR ID - 1582 TI - Salmonella enterica serovar Enteritidis live vaccine strain in the reproductive organs of laying goose after subcutaneous vaccination JO - Iranian Journal of Veterinary Research JA - IJVR LA - en SN - 1728-1997 AU - He, G. Z. AU - Deng, S. X. AU - Tian, W. Y. AD - Department of Microbiology, Guiyang College of Traditional Chinese Medicine, Guiyang 550002, Guizhou Province, China AD - Agricultural Office of Dalingshan Town, Dongguan 523830, Guandong Province, China Y1 - 2013 PY - 2013 VL - 14 IS - 2 SP - 100 EP - 105 KW - Salmonella Enteritidis KW - Reproductive organs KW - Population KW - Goose DO - 10.22099/ijvr.2013.1582 N2 - Serovar-specific real-time PCR for Salmonella enterica serovar Enteritidis (S. Enteritidis) was conductedto detect the genomic DNA of S. Enteritidis from laying goose after subcutaneous vaccination at differenttime points. Indirect fluorescent antibody (IFA) technique and immunohistochemical localization wereemployed to validate the results. The results showed that S. Enteritidis was consistently detected in all thesamples. Vagina and uterus were positive at 20 h PI, and the last organ to show a positive result was thelargest and third largest preovulatory follicle, at 32 h PI. The copy numbers of S. Enteritidis DNA in eachtissue reached a peak at 36-60 h PI, with the vagina and uterus containing higher concentrations than othertissues. However, the number of bacteria started decreasing by 3-4 d, and by 6 d, the concentration of S.Enteritidis DNA was below the detection limits of the PCR assay, except the vagina. The real-time PCRanalysis of a variety of tissues is significant for further investigation of the mechanism of vaccine protectionand the optimization of vaccination regimes. UR - https://ijvr.shirazu.ac.ir/article_1582.html L1 - https://ijvr.shirazu.ac.ir/article_1582_5aacb53b1752aeb6d1cebf8e17f60ecc.pdf ER -