TY - JOUR ID - 1477 TI - An experimental study on early pathogenesis of a very mvirulent isolate of infectious bursal disease virus, employing immunohistochemistry JO - Iranian Journal of Veterinary Research JA - IJVR LA - en SN - 1728-1997 AU - Siavosh Haghighi, Z. M. AU - Tavasoly, A. AU - Marjanmehr, S. H. AU - Shoshtary, A. AU - Bahmaninejad, M. A. AD - Department of Pathobiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran AD - Department of Poultry Diseases, Razi Vaccine and Serum Research Institute, Karaj, Iran Y1 - 2009 PY - 2009 VL - 10 IS - 2 SP - 125 EP - 131 KW - Infectious bursal disease KW - Very virulent infectious bursal disease virus KW - Pathogenesis KW - Immunohistochemistry DO - 10.22099/ijvr.2009.1477 N2 - In this study, immunohistochemistry was used to clarify the early stages of viral kinetics and cycliccourse of IBDV, IR499, which has been described earlier as a very virulent strain (vvIBDV). Fifteen, 4-week-old SPF chickens were inoculated with 10³ EID50 of vvIBDV, IR499, via oculo/nasal route. Five birdsserved as controls, and inoculated with phosphate buffered saline (PBS). The birds were then bled, and tissue samples from bursa of Fabricius, cecal tonsils, liver, spleen, thymus and thigh muscle were harvested at 3, 6, 12, 24 and 48 h post-inoculation (p.i.). Typical positive signals were first observed as early as 3 h p.i. in lymphoid cells of cecal tonsils (the organ of primary affinity) and Kupfer cells of liver. Viral antigens inbursa were first found at 6 h p.i. which represents the occurrence of primary viraemia. After secondaryviraemia, the virus appeared in spleen and thymus at 12 h p.i. These findings at early stages of viral infection, represented IBDV, IR499, as a very virulent strain with a rapid and generalized course, at in vivo level. UR - https://ijvr.shirazu.ac.ir/article_1477.html L1 - https://ijvr.shirazu.ac.ir/article_1477_f1667f4d500817ddc54b23da0d29ce7e.pdf ER -