TY - JOUR ID - 1082 TI - Equine marrow-derived mesenchymal stem cells: isolation, differentiation and culture optimization JO - Iranian Journal of Veterinary Research JA - IJVR LA - en SN - 1728-1997 AU - Baghaban Eslaminejad, M. R. AU - Taghiyar, L. AU - Dehghan, M. M. AU - Falahi, F. AU - Kazemi Mehrjerdi, H. AD - Department of Stem Cell, Cell Sciences Research Centre, Royan Institute, ACECR, Tehran, Iran AD - Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran AD - Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran Y1 - 2009 PY - 2009 VL - 10 IS - 1 SP - 1 EP - 11 KW - Equine MSCs isolation KW - Cartilage KW - Bone KW - Adipose differentiation KW - FBS concentration DO - 10.22099/ijvr.2009.1082 N2 - Most studies regarding the marrow-derived equine mesenchymal stem cells (MSCs) have mainly focusedon the cell transplantation without considering the capacity of differentiation and in vitro requirements of thecells. These concerns were investigated in the present study. Equine MSCs were isolated from the sternalmarrow aspirates and expanded through two successive subcultures. Passage-2 equine MSC cultures werethen treated with appropriate supplements in order to examine the cell osteogenic, chondrogenic andadipogenic differentiation potential. Furthermore, the culture of the cells was investigated in terms of theoptimal concentration of fetal bovine serum (FBS) and the initial cell-seeding density. Additionally, a growthcurve was plotted for the cells to study their growth characteristics. According to our findings, equine MSCswere easily generated specialized bone, cartilage and adipose cell lineages as confirmed by specific stainingand RT-PCR analysis. Moreover, the cells exhibited rapid expansion when being cultivated in the mediumwith 15% FBS at 100 cells/cm2. Growth curves indicated that these cells rapidly entered the log phase after abrief lag (adaptation) period. In summary, marrow-derived equine MSCs possess tripotent differentiationcapacity and rapid growth rate in the appropriate culture conditions. UR - https://ijvr.shirazu.ac.ir/article_1082.html L1 - https://ijvr.shirazu.ac.ir/article_1082_76b8a461f768e9c8d45f392bca09d0d1.pdf ER -