Shiraz University
Iranian Journal of Veterinary Research
1728-1997
2252-0589
19
4
2018
12
01
Effects of chitosan scaffold along with royal jelly or bee venom in regeneration of critical sized radial bone defect in rat
246
254
EN
A.
Meimandi-Parizi
Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
meimandi@shirazu.ac.ir
A.
Oryan
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
A.
Bigham-Sadegh
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
E.
Sayahi
Resident of Veterinary Surgery, Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
10.22099/ijvr.2018.5052
<span lang="EN">The aim of this study was to compare the efficacy of honey bee venom (BV) and royal jelly (RJ) alongside chitosan scaffold (CS) in improving radius bone defect in rats. <span class="alt-edited">A total of 60 full thickness radial bone defects with a length of 5 mm were created in 60 male Wistar rats. Six healthy radial bones (3 rats) were also assigned as normal control for biomechanical studies. The defects were left empty (untreated group) or were filled by the autograft (autograft group), CS (CS group), </span>CS alongside the BV solution (CS-BV group), and CS alongside the RJ solution (CS-RJ group). <span class="alt-edited">Healing of the bone defects were evaluated clinically and radiologically</span> on days 0, 28, 42 and 56 after operation<span class="alt-edited"> while the biomechanical testing and histopathological examination were performed on the 56th day after surgery.</span></span><span> The autograft was more radiopaque than the untreated and CS groups at the 28th, 42nd and 56th postoperative days (P<0.05). The CS-BV and CS-RJ groups showed significantly higher radiographic outcomes than the untreated and CS groups at the 56th post-operative day (P<0.05). The density of osseous tissue (DOT) and the osteocytes and osteoblasts count of the CS-RJ and CS-BV groups were significantly higher than the CS and autograft groups (P<0.05). The biomechanical results of the CS-RJ group were significantly superior to the autograft, while the biomechanical properties of CS-BV group were not significantly different with the autograft group (P>0.05). </span><span lang="EN">The scaffolds in CS group were observable in the surgical site after 56 days. </span><span>There was no significant difference in radiographs, DOT, cartilage tissue and fibrous tissue, and also biomechanical performances of the CS-BV and CS-RJ groups at the 42nd and 56th day after surgery. </span><span>The untreated and CS groups showed weakest biomechanical results among all groups. </span><span>It could be concluded that both </span><span lang="EN">treatment strategies in the CS-BV and CS-RJ groups were appropriate and useful in treating critical bone defects</span><span>.</span>
Bee venom,Bone healing,Chitosan,Royal jelly
https://ijvr.shirazu.ac.ir/article_5052.html
https://ijvr.shirazu.ac.ir/article_5052_674f9814bb690ef8d9dcd2b6707d40f9.pdf
Shiraz University
Iranian Journal of Veterinary Research
1728-1997
2252-0589
19
4
2018
12
01
Pendrin and sodium/iodide symporter protein expression in the testicular tissue of normal and diabetic rats in prepubertal and post pubertal stages
255
261
EN
T.
Karaca
0000-0002-2500-7781
Department of Histology and Embryology, Faculty of Medicine, Trakya University, Balkan Campus, 22030, Edirne, Turkey
turankaraca74@hotmail.com
S.
Demirtas
Department of Histology and Embryology, Faculty of Medicine, Trakya University, Balkan Campus, 22030, Edirne, Turkey
D.
Uzun Goren
Department of Histology and Embryology, Faculty of Medicine, Trakya University, Balkan Campus, 22030, Edirne, Turkey
10.22099/ijvr.2018.5053
<span lang="EN-GB">Pendrin (PDS) and sodium/iodide symporter (NIS) are transmembrane proteins that are located in numerous tissue types, particularly thyroid follicular epithelial cells, where they are entrusted with the regulation of iodine molecules. In the present study, we aimed to clarify changes in PDS and NIS protein expression, in the testicular tissue of prepubertal and </span><span lang="EN-GB">post pubertal </span><span lang="EN-GB">rats at normal or diabetic conditions. </span><span lang="EN-GB">Forty Wistar albino male rats (20 prepubertal and 20 </span><span lang="EN-GB">post pubertal</span><span lang="EN-GB">) were divided into four groups, as follows: group I was prepubertal control, group II was prepubertal diabetic (60 mg/kg intraperitoneal [ip] streptozotocin [STZ]), group III was post pubertal control, and group IV was post pubertal diabetic (60 mg/kg ip STZ). </span><span lang="EN-GB">Ki67 immunoreactivity decreased in testicular tissue of both the prepubertal and post pubertal </span><span lang="EN-GB">diabetic </span><span lang="EN-GB">groups; the apoptotic tubule index and apoptotic cell number increased in the </span><span lang="EN-GB">diabetic </span><span lang="EN-GB">groups as compared to the control groups. </span><span lang="EN-GB">Pendrin</span><span lang="EN-GB"> immunoreactivity was detected in seminiferous tubules and Leydig cells; and was significantly reduced in the </span><span lang="EN-GB">diabetic </span><span lang="EN-GB">groups (P<0.05). The number of cells positive for NIS was significantly decreased in prepubertal and post pubertal rats with diabetes, compared to the controls. Enzyme-linked immunosorbent assay (ELISA) analysis showed that PDS and NIS values were significantly reduced in the prepubertal and post pubertal diabetic groups as compared to the control groups. </span><span lang="EN-GB">Our results indicate a potential relationship between puberty and PDS and NIS expression in rat testicular tissue and showed the decreasing effects of diabetes on PDS and NIS expression in testicular tissues in rats.</span>
Diabetes,Pendrin,Rat,Sodium/iodide symporter,Testis
https://ijvr.shirazu.ac.ir/article_5053.html
https://ijvr.shirazu.ac.ir/article_5053_86c78e361019feb4bf0e959d12f1e163.pdf
Shiraz University
Iranian Journal of Veterinary Research
1728-1997
2252-0589
19
4
2018
12
01
Mycobacterium avium subsp. paratuberculosis induces differential cytosine methylation at miR-21 transcription start site region
262
269
EN
S.
Mostoufi-Afshar
Graduated from School of Veterinary Medicine, Shiraz University, Shiraz, Iran
M.
Tabatabaei
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
M. M.
Ghahramani Seno
Department of Basic Sciences,
Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran and Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran
mgseno@um.ac.ir
10.22099/ijvr.2018.5054
<em><span lang="EN-CA">Mycobacterium avium</span></em><span>subspecies</span><span> <em><span lang="EN-CA">paratuberculosi</span></em><span lang="EN-CA">s (MAP), as an obligate intracellular bacterium, causes <em>paratuberculosis</em> (Johne’s disease) in ruminants. Plus, MAP has consistently been isolated from Crohn’s disease (CD) lesions in humans; a notion implying possible direct causative effect for MAP in CD development. Infections caused by MAP are refractory to treatment and in many cases the treatment does not easily resolve the infection. Studying the molecular mechanisms of host-pathogen interaction is helpful in identifying possible drug targets. In this line, it has already been shown that in macrophages infected with various bacteria, including mycobacteria, micro RNA 21 (miR-21) is upregulated, a change that results in diminished macrophages clearance ability and favours pathogens survival within the cells. However, the molecular mechanism(s) by which the intracellular bacteria induce miR-21 expression is not known. In order to verify possible effects from epigenetic changes induced by intracellular bacteria, we studied the cytosine methylation changes at the transcription start regions of miR-21 in THP-1 macrophages infected with MAP. For this purpose, genomic DNA was extracted from infected cells and the methylation status at the region of interest was evaluated by bisulfite conversion method. Our work showed that MAP directs de-methylation of the cystosines atCpG di-nucleotides in this region, while non-CpG cytosines of this region did not show significant changes. Interestingly, the CpG cytosines that were differentially methylated in the infected macrophages occur at the binding sites of the transcription factors already known to regulate miR-21 expression.</span></span>
Crohn’s disease,Johne’s disease,miR-21,Mycobacterium
https://ijvr.shirazu.ac.ir/article_5054.html
https://ijvr.shirazu.ac.ir/article_5054_e3145406905339a82eca9483000aefad.pdf
Shiraz University
Iranian Journal of Veterinary Research
1728-1997
2252-0589
19
4
2018
12
01
Evaluation of semen characteristics, oxidative stress, and biochemical indices in Arabian horses of different ages during the hot summer season
270
275
EN
M. M. M.
Kandiel
Theriogenology Department, Faculty of Veterinary Medicine, Benha University, Benha, Egypt
mohamed.kandil@fvtm.bu.edu.eg
A. R. M.
El Khawagah
Theriogenology Department, Faculty of Veterinary Medicine, Benha University, Benha, Egypt
10.22099/ijvr.2018.5055
<span>Egypt is anticipated to be potentially influenced by the global climate warming. Therefore, the current study aimed to evaluate the influence of age on the fertility potential of Arabian stallions during summer breeding months. Arabian horses grouped according to their age into three groups, each involved six stallions: young (5-6 years), middle (11-12 years) and old (15-20 years) age groups, were weekly sampled during the months of July-August. Ejaculates were collected using artificial vagina, Missouri model, and examined for pH, volume, concentration, motility, livability and morphological abnormalities. Serum samples were harvested and assessed for testosterone, total antioxidant capacity (TAC), lipid profile, and copper and zinc levels. Semen pH (P<0.005), spermatozoa motility (P=0.08), sperm morphology (P<0.001), tail abnormalities (P<0.001), and sperm count per ejaculate differed noticeably between stallions’ groups. Testosterone (P=0.07) and TAC (P<0.05) concentrations were markedly affected by stallions’ age. Cholesterol correlated negatively with sperm normality, but serum copper and zinc levels correlated positively with semen volume, sperm cell count and spermatozoa livability. These results revealed that the fertility of stallions is age-dependent and is prominently influenced by lipid metabolism and oxidative stress during hot summer breeding season. It is highly advisable to provide animals’ house (along with feed and drinking water) with the evaporative cooling system and allow morning or late afternoon outdoor activity to bypass the summer hot climates and sustain stallions’ fertility.</span>
Arabian stallion,Oxidative stress,Semen,Summer season,Testosterone
https://ijvr.shirazu.ac.ir/article_5055.html
https://ijvr.shirazu.ac.ir/article_5055_15683319c997151644ab7c0bb5a4432b.pdf
Shiraz University
Iranian Journal of Veterinary Research
1728-1997
2252-0589
19
4
2018
12
01
Effect of diets containing roasted soybean, extruded soybean or their combination on performance and milk fatty acid profile of lactating Holstein cows
276
282
EN
F.
Fatahnia
Department of Animal Science, Faculty of Agriculture, Ilam University, Ilam, Iran
f.fatahnia@ilam.ac.ir
A.
Azarfar
Department of Animal Science, Faculty of Agriculture, Lorestan University, Khorramabad, Iran
S.
Menatian
Department of Animal Science, Faculty of Agriculture, Zanjan University, Zanjan, Iran
Sh.
Joz Ghasemi
Department of Animal Science, Faculty of Agriculture, Zanjan University, Zanjan, Iran
A.
Poormalekshahi
Department of Animal Science, Faculty of Agriculture, Ilam University, Ilam, Iran
A. N.
Shokri
Department of Animal Science, Faculty of Agriculture, Ilam University, Ilam, Iran
10.22099/ijvr.2018.5056
<span>The aim of this study was to investigate the effect of diets containing roasted soybean (R), extruded soybean (E) or their combination (RE) on dairy cow performance and milk fatty acid (FA) profile. Nine multiparous lactating Holstein cows (680 ± 25 kg BW; 90 ± 10 DIM; means ± SD) were randomly assigned to a triple 3 × 3 Latin square design. Dry matter intake (DMI), apparent nutrient digestibility, milk yield, composition and FA profile and efficiency of nitrogen (N) utilization for milk production were measured. Cows fed R and RE had higher 4% fat corrected milk (FCM) yield and DMI compared to those fed the E (P<0.05). The efficiency of 4% FCM production per unit of DMI was not influenced by experimental diets (P>0.05). Experimental diets had no effect on milk fat and protein concentrations and nutrient digestibility (P>0.05). Milk fat concentrations of vaccenic acid (VA; C18:1 trans-11) and conjugated linoleic acid (CLA; C18:2 cis-9, trans-11) were higher for cows fed with RE compared to other groups (P<0.05). We concluded that cows fed with RE had similar milk production and fat content compared to those fed with R and a similar beneficial milk FA profile compared to those fed with E. Thus, feeding a diet with RE improved the quality and quantity of dairy cow milk.</span>
Animal performance,Extruded soybean,Lactating cow,Milk fatty acid profile,Roasted soybean
https://ijvr.shirazu.ac.ir/article_5056.html
https://ijvr.shirazu.ac.ir/article_5056_7cc1063ef6fe00fdb3c7bc2baa5a5fb1.pdf
Shiraz University
Iranian Journal of Veterinary Research
1728-1997
2252-0589
19
4
2018
12
01
Quantification and comparison of TLR2 activity in monocyte-derived macrophages of zebu and crossbred cattle
283
289
EN
A.
Baranwal
Division of Animal Genetics, ICAR-Indian Veterinary Research Institute, Izatnagar-243122, Bareilly, Uttar Pradesh, India
A.
Sonwane
Division of Animal Genetics, ICAR-Indian Veterinary Research Institute, Izatnagar-243122, Bareilly, Uttar Pradesh, India
arvind.sonwane@icar.gov.in
A.
Chauhan
Division of Animal Genetics, ICAR-Indian Veterinary Research Institute, Izatnagar-243122, Bareilly, Uttar Pradesh, India
M.
Panigrahi
Division of Animal Genetics, ICAR-Indian Veterinary Research Institute, Izatnagar-243122, Bareilly, Uttar Pradesh, India
A. K.
Sharma
Division of Animal Genetics, ICAR-Indian Veterinary Research Institute, Izatnagar-243122, Bareilly, Uttar Pradesh, India
10.22099/ijvr.2018.5057
<span>The present study was conducted to quantify and compare TLR2 (toll-like receptor 2) activity in monocyte-derived macrophages of zebu (Tharparkar) and crossbred (Holstein-Friesian × Jersey × Brown Swiss × Hariana) cattle. The cells were either induced with Pam3CSK4 or kept as control. The TLR2 activity was quantified in terms of IκB-α inhibitory subunit (NFKBIA) messenger RNA (mRNA) copies using real-time, one-step reverse transcription-polymerase chain reaction (RT-PCR). Toll-like receptor 2 activity of induced cells was in the range of 1060421 ± 477937 (n=3) to 3514715 ± 290222 (n=3) copies for Tharparkar cattle (n=7) and in the range of 1365532 ± 47243 (n=3) to 3016510 ± 172340 (n=3) copies for the crossbred cattle (n=7). For uninduced cells, this activity was within the range of 117 ± 51 (n=3) to 293 ± 103 (n=3) copies for the Thraparkar cattle (n=7), and in the range of 182 ± 122 (n=3) to 296 ± 88 (n=3) copies for the crossbred cattle (n=7). The TLR2 activity of induced cells in both groups was found to be significantly higher than that of the respective uninduced cells (P<0.0001). Furthermore, upon comparison, TLR2 activities of induced and uninduced cells of the Tharparkar were not found to be significantly different from those of the crossbred cattle (P=0.8154 and P=0.6670). In the present study, we have quantified and compared, for the first time, TLR2 activity in terms of NFKBIA mRNA copies in monocyte-derived macrophages of Tharparkar and crossbred cattle and found that both have equivalent TLR2 activity.</span>
Crossbred,Monocyte-derived macrophages,NFKBIA,Tharparkar,TLR2
https://ijvr.shirazu.ac.ir/article_5057.html
https://ijvr.shirazu.ac.ir/article_5057_f8ac489bf1d92b290be5db3e471a73fb.pdf
Shiraz University
Iranian Journal of Veterinary Research
1728-1997
2252-0589
19
4
2018
12
01
Improving agricultural production of domestic rabbits in Serbia by follow-up study of their parasitic infections
290
297
EN
T.
Ilić
Department of Parasitology, Faculty of Veterinary Medicine, University of Belgrade, 11000 Beograd, Serbia
P.
Stepanović
Department of Equine, Small Animal, Poultry and Wild Animal Diseases, Faculty of Veterinary Medicine, University of Belgrade, 11000 Beograd, Serbia
pedja@vet.bg.ac.rs
K.
Nenadović
Department of Animal Hygiene, Faculty of Veterinary Medicine, University of Belgrade, 11000 Beograd, Serbia
S.
Dimitrijević
Department of Parasitology, Faculty of Veterinary Medicine, University of Belgrade, 11000 Beograd, Serbia
10.22099/ijvr.2018.5058
<span>A study was made on the prevalence of some parasitic infections appearing in domestic rabbits obtained from individual breeders in Serbia in order to improve the agricultural production of these animals. Aside from economic reasons (meat production and reproduction) rabbits are bred for the research purposes, and races are kept as household pets. For these reasons, among others, it is important to gain knowledge of medical culprits including causes of parasitic diseases that compromise their health, well-being and cause economic losses. This parasitological research was conducted in the period from 2010 </span><span lang="EN-GB">to 2015 </span><span>in 8 epidemiological regions of Serbia, on 433 rabbits as </span><span lang="EN">representative </span><span>samples of different races </span><span>(154 individuals up to 1 years of age and 279 individuals older than 5). Out of the total number of examined rabbits parasitic infections were established in 82.68% of animals. We detected 3 species of endoparasites (<em>Eimeria</em> spp., <em>Trichostrongylus</em> spp., and <em>Passalurus ambiguus</em>) and 3 species of ectoparasites (Scabies from genera <em>Sarcoptes</em>, <em>Psoroptes</em> and <em>Notoedres</em>). In “kits” (small rabbits) coccidiosis was the most prevalent disease (50.65%), while in older animals trichostrongilidosis was common (39.07%). </span><span lang="EN">The most represented </span><span>scabies infection was with the species <em>Psoroptes cuniculi</em> (12.01%). Aiming at better control on the health of rabbits, there is a growing need for continual monitoring of parasitic infections including appropriate diagnosis, application efficient therapeutic protocols and control measures.</span>
Domestic rabbits,Individual breeders,Parasitic infection,Serbia
https://ijvr.shirazu.ac.ir/article_5058.html
https://ijvr.shirazu.ac.ir/article_5058_6fbd8b32f0ee3cd2a6a01a2722c1ad6c.pdf
Shiraz University
Iranian Journal of Veterinary Research
1728-1997
2252-0589
19
4
2018
12
01
Different cell death types determination in juvenile mice ovarian follicles
298
303
EN
M.
Pajokh
Graduated from School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
F.
Mesbah
Department of Anatomical Sciences, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
H.
Bordbar
Department of Anatomical Sciences, School of Medicine, Shiraz University of Medical Sciences, Histomorphometry & Stereology Research Center, Shiraz, Iran
T.
Talaei-Khozani
Department of Anatomical Sciences, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
talaeit@sums.ac.ir
10.22099/ijvr.2018.5059
<span>Follicular atresia </span><span>is a phenomenon that leads to </span><span>evacuation of the </span><span>ovary from the oocytes and the occurrence of menopause. The contribution of various types of cell death in atresia at different follicular developmental stages requires extensive investigation. In this study, we </span><span>evaluated </span><span>3 types of </span><span>programmed </span><span>cell death (PCD), apoptosis, autophagy, and necrosis, in juvenile mouse ovary when we can observe </span><span>all follicular stages as well as atresia.</span><span> Ovaries from <span>juvenile</span> mice on the 21st post-natal (PN) day were prepared histologically for <span>terminal deoxynucleotidyl transferase dUTP nick end labeling</span> </span><span>(TUNEL) to evaluate apoptosis and immunohistochemistry for beclin-1 to evaluate the autophagy marker. Necrotic cell death was also assessed by penetration of </span><span>propidium iodide (</span><span>PI). The count and percentage </span><span>of the labeled follicles at different stages in the ovaries</span><span> were evaluated</span><span> and compared using the Kruskal-<em><span>Wallis and Mann-Whitney tests</span></em>. </span><span>We detected TUNEL-positive </span><span>granulosa cells in pre-antral and antral follicles but not in the primordial and primary follicles. Somatic cells and oocytes of primordial, primary, pre-antral and antral follicles reacted to </span><span>beclin-1. </span><span>The percentage of the </span><span>PI-labeled primordial and primary follicles were significantly higher than the </span><span>beclin-1 positive </span><span>(<span>P=0.01 and P=0.01</span>). </span><span>In conclusion, we showed that apoptosis, autophagy, and necrosis play a role in follicular atresia</span><span> and the contributions of each one depends on the follicular stages</span><span>. It was also demonstrated that necrosis happens particularly in the small follicles while in the large one, all three cell death types occurred with an equal ratio.</span>
Apoptosis,Autophagy,Mouse,Necrosis,Ovary
https://ijvr.shirazu.ac.ir/article_5059.html
https://ijvr.shirazu.ac.ir/article_5059_1f24d5d51ce4d4d5804f94c646236a10.pdf
Shiraz University
Iranian Journal of Veterinary Research
1728-1997
2252-0589
19
4
2018
12
01
Abnormal renovascular resistance in dogs with diabetes mellitus: correlation with glycemic status and proteinuria
304
309
EN
M.
Priyanka
Animal Experimentation Station, Indian Veterinary Research Institute, Regional Campus, Bengaluru, Karnataka, 560024, India
bidarvet@gmail.com
K.
Jeyaraja
Department of Clinics, Madras Veterinary College, TANUVAS, Chennai-600007, India
P. S.
Thirunavakkarasu
Department of Veterinary Clinical Medicine, Ethics and Jurisprudence, Madras Veterinary College, TANUVAS, Chennai-600007, India
10.22099/ijvr.2018.5060
<span>Present study was conducted with the objectives of determining the renal vascular resistance in dogs with diabetes mellitus and to study the correlation between the indices of renovascular resistance with glycemic status, systolic blood pressure (SBP) and proteinuria in dogs with diabetes mellitus. This study was conducted on seventeen diabetic dogs and ten apparently healthy dogs. Increased renal resistive index (RI) and pulsatility index (PI) were observed in diabetic dogs as compared to healthy dogs. Systemic hypertension and proteinuria were observed in 10 and 3 out of 17 diabetic dogs, respectively. Significant positive correlation was observed between the indices of renovascular resistance and fasting blood glucose levels and between the indices of renovascular resistance and serum glycated hemoglobin levels. No correlation was observed between the indices of renovascular resistance and SBP as well as the indices of renovascular resistance and proteinuria. As the indices of renovascular resistance correlate significantly with glycemic status, they can be used as the early marker for kidney damage in diabetic patients. Among these indices renal PI was found to be more sensitive than renal RI.</span>
Diabetes mellitus,dog,Glycemic status,Proteinuria,Renovascular resistance
https://ijvr.shirazu.ac.ir/article_5060.html
https://ijvr.shirazu.ac.ir/article_5060_78580e21387d83d8c17cad847b09014f.pdf
Shiraz University
Iranian Journal of Veterinary Research
1728-1997
2252-0589
19
4
2018
12
01
The effect of adding pomegranate seed extract to fat-containing diets on nutrients digestibility, intestinal microflora and growth performance of broilers
310
317
EN
M. R.
Rezvani
Department of Animal Science, College of Agriculture, Shiraz University, Shiraz, Iran
rezvani@shirazu.ac.ir
N.
Sayadpour
Graduated from College of Agriculture, Shiraz University, Shiraz, Iran
F.
Saemi
Graduated from College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
10.22099/ijvr.2018.5061
<span>The present study was aimed at </span><span>studying the effects of inclusion of </span><span>pomegranate seed extract to fat-containing diets on nutrients digestibility, intestinal microflora and performance of broilers.</span><span> A total of 320 one-day-old broiler chicks (Ross 308) were selected in a 2 × 2 × 2 factorial experiment in a completely randomized design with 4 replicates of 10 birds of each. The dietary treatments consisted of pomegranate seed extract (0 and 2%), commercial Nutriad<sup>®</sup> antioxidant (0 and 0.01%) and soybean oil without antioxidant (0 and 6% during the grower period and 0 and 8% for finisher period). The chicks were reared from 1 to 10 days in one group. On the eleventh day, 10 chicks per pen were randomly allocated to each cage per replicate. Data were performed in generalized linear model (GLM) procedures in SAS at 5% level of significance and the least square means correction after Tukey’s test was used. Commercial antioxidant improved crude fat digestibility and decreased population of caecum <em>Lactobacillus</em>, but pomegranate seed extract improved crude fat digestibility and increased caecum <em>Lactobacillus</em> population. Due to positive effects of pomegranate seed extract in fat containing diets on fat digestibility and <em>Lactobacillus</em> bacteria in caecum, it could be concluded that using pomegranate seed extract in fat containing diets could be useful.</span>
Antioxidants,Broiler,Digestibility,Performance,Pomegranate seed extract
https://ijvr.shirazu.ac.ir/article_5061.html
https://ijvr.shirazu.ac.ir/article_5061_847187bbbeba3a9109b682e6c39ea5a6.pdf
Shiraz University
Iranian Journal of Veterinary Research
1728-1997
2252-0589
19
4
2018
12
01
Accidental acetamiprid poisoning in a buffalo
318
320
EN
N.
Kushwaha
0000-0002-4440-5590
Department of Teaching Veterinary Clinical Complex, College of Veterinary and Animal Sciences, Udgir-413 517, Maharashtra Animal and Fishery Sciences University, Maharashtra, India
kushneils@gmail.com
A.
Mohan
Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary and Animal Sciences, Udgir-413 517, Maharashtra Animal and Fishery Sciences University, Maharashtra, India
A. U.
Bhikane
Department of Clinical Medicine, Ethics and Jurisprudence, College of Veterinary and Animal Sciences, Udgir-413 517, Maharashtra Animal and Fishery Sciences University, Maharashtra, India
10.22099/ijvr.2018.5062
<span>Acetamiprid is a first generation systemic neonicotinoid insecticide, routinely used for crop protection against sucking type insects. It is likely to be of low toxicity in mammals but severe poisoning may occur if ingested in large amount. A case of buffalo with accidental ingestion of acetamiprid was presented with severe gastrointestinal symptoms and respiratory distress. The patient was managed successfully with symptomatic and supportive treatment. As far as the present report is concerned, it is the first report of acute acetamiprid poisoning in buffalo from India. From this report it is concluded that awareness programs about safe use of pesticides should be implemented.</span>
Acetamiprid,Buffalo,Neonicotinoid
https://ijvr.shirazu.ac.ir/article_5062.html
https://ijvr.shirazu.ac.ir/article_5062_85a6f152742c25ab1f88454fa5f43a21.pdf
Shiraz University
Iranian Journal of Veterinary Research
1728-1997
2252-0589
19
4
2018
12
01
Diagnosis and successful management of a rare case of phaeohyphomycosis in a Doberman pinscher dog
321
324
EN
V. K.
Singh
0000-0002-9229-5162
Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, DUVASU, Mathura, Uttar Pradesh-281001, India
vet.vinodsingh@gmail.com
A.
Kumar
Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, DUVASU, Mathura, Uttar Pradesh-281001, India
R. P.
Pandey
Teaching Veterinary Clinical Complex, College of Veterinary Science and Animal Husbandry, DUVASU, Mathura, Uttar Pradesh-281001, India
S. K.
Yadav
Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, DUVASU, Mathura, Uttar Pradesh-281001, India
10.22099/ijvr.2018.5063
<span>Skin affections in canines are common and their treatment depends upon the pathogens involved. Delayed or no response to traditional treatment indicates the involvement of unusual etiology or the multidrug resistance. This report describes the diagnosis and therapeutic management of a rare case of phaeohyphomycosis caused by <em>Alternaria alternata</em> complicated with <em>Pseudomonas aeruginosa</em> associated pyoderma in a Doberman pinscher dog. Identification of the etiological agents was based on microscopy and culture of the skin lesions and further confirmation by polymerase chain reaction (PCR) amplification. Treatment consisted of a combination of systemic antifungal and antibacterial drugs selected on the basis of <em>in vitro</em> antimicrobial susceptibility tests along with the tropical therapy. The report hightlights the need of studies on opportunistic fungi to better understand the associated risks, pathogenesis and optimal treatment of these uncommon infections.</span>
Alternaria,Concurrent,dog,Phaeohyphomycosis,Pseudomonas
https://ijvr.shirazu.ac.ir/article_5063.html
https://ijvr.shirazu.ac.ir/article_5063_f2ecc85b783c0c6130032527e2b06b97.pdf