Shiraz University
Iranian Journal of Veterinary Research
1728-1997
2252-0589
7
1
2006
03
01
Comparison of optic lens proteins among animals at different stages of development
1
7
EN
M.
Aminlari
Department of Biochemistry, School of Veterinary Medicine, University of Shiraz, Shiraz, Iran
aminlari@hafez.shirazu.ac.ir
S.
Gholami
Department of Anatomical Sciences, School of Veterinary Medicine, University of Shiraz, Shiraz, Iran
Gh.
Parhizgari
Graduated from School of Veterinary Medicine, University of Shiraz, Shiraz, Iran
S.
Ranjbar
Graduated from School of Veterinary Medicine, University of Shiraz, Shiraz, Iran
10.22099/ijvr.2006.2672
The purpose of this investigation was to study and compare the electrophoretic patterns of optic lens<br />proteins of different species of domestic animals at pre- and post-natal ages. Optic lenses were removed from the embryo or adult sheep, cattle, goat, camel and chicken at the slaughter-house then homogenized and subjected to sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE). In all animals, except chicken, majority of proteins had molecular weights of less than 33 kDa and their concentrations were not affected by the age of animals at pre- or post-natal stages. A 9 kDa protein which was present in adult sheep optic lens was absent in sheep fetal lenses at different age groups. Prominent differences were observed in camel and chicken. In camel, proteins with molecular weights of 30 and 38 kDa were present, the concentration of which was much lower in other animals. A protein of 57 kDa which constituted the major<br />protein of chicken optic lens was absent in other species of animals. The concentration of proteins in the<br />range of 25-30 kDa increased with the age of chicken embryos. These proteins were remarkably different<br />from those of adult chicken lens proteins except the 57 kDa protein which was also the predominant protein<br />in the embryo. The 38 kDa protein disappeared and a 20 kDa protein appeared in the chicken embryo lens as compared with adult chicken lens. These data indicate extensive differences in the lens proteins of animals and suggest different physiological functions of lens proteins in different animals at different stages of development.
Domestic animals,Crystallins,Optic lens,SDS-PAGE
https://ijvr.shirazu.ac.ir/article_2672.html
https://ijvr.shirazu.ac.ir/article_2672_3185fe6f3bc342944f8342d539a94bba.pdf
Shiraz University
Iranian Journal of Veterinary Research
1728-1997
2252-0589
7
1
2006
03
01
In vitro survival rate of bovine oocytes following vitrification in glass capillary micropipette (GCM)
8
13
EN
H.
Babaei
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
babaei@mail.uk.ac.ir
S. N.
Nematollahi Mahani
Department of Anatomical Sciences, Afzalipour School of Medicine, University of Medical Sciences of Kerman, Kerman, Iran
A.
Kheradmand
Department of Clinical Sciences, College of Veterinary Medicine,
University of Lorestan, Khorram Abad, Iran
kheradmand_a@lu.ac.ir
E.
Ayen
Department of Clinical Sciences, Faculty of Veterinary
Medicine, University of Urmia, Urmia, Iran
e.ayen@mail.urmia.ac.ir
10.22099/ijvr.2006.2673
The purpose of this study was to evaluate the use of glass capillary micropipette (GCM) as a vessel for<br />vitrification of bovine oocytes. Cumulus-oocyte complexes (COCs) were obtained from slaughter-house and<br />washed 5 to 6 times in the washing medium (TCM-199 + 20% FBS) and randomly assigned to treatment and<br />control group. In the first step of vitrification, COCs were exposed to first vitrification solution (VS1) (10%<br />ethylene glycol (EG), 10% DMSO in holding medium (TCM-199 + 10% FBS: HM)) for 1 min at room<br />temperature and then placed in VS2 solution (20% EG, 20% DMSO in HM) for 25 sec and immediately were<br />loaded into the GCM vessel. The filled portion of GCM vessels were placed in liquid nitrogen (LN2) for 3 to<br />5 sec and then completely immersed and stored there. The oocytes were thawed by immersing the capillary<br />end of the straw in 1 ml of 0.25 M sucrose in HM and gently expelling the contents. After 1 min the oocytes<br />were transferred into 100 μl of 0.15 M sucrose in HM for another 5 min and then washed with HM twice. For<br />examining the in vitro developmental potential of vitrified-warmed oocytes, the oocytes were placed in 50 μl<br />droplet of maturation medium (TCM-199 + 10% FBS + 10 IU/ml PMSG + 5 IU/ml HCG) covered with<br />paraffin oil in a CO2 incubator at 38.5ºC for 24 hrs. A high proportion of morphologically normal oocytes<br />(90%) was recovered after vitrification-warming. The percentage of live oocytes after 24 hrs when tested<br />with trypan blue in GCM group was 85.18%, significantly did not differ from control group (90%). The<br />proportion of oocytes which were found to have undergone nuclear maturation did not show statistical<br />difference between the control and GCM group (61.29% vs 40%, respectively). The results of present study<br />demonstrated that vitrification of immature bovine oocytes in the GCM vessels and EG + DMSO solution<br />have high survival rate.
Vitrification,Bovine,Oocyte,Glass capillary micropipette
https://ijvr.shirazu.ac.ir/article_2673.html
https://ijvr.shirazu.ac.ir/article_2673_8299076f772002a6bb23719110bcb3dc.pdf
Shiraz University
Iranian Journal of Veterinary Research
1728-1997
2252-0589
7
1
2006
03
01
Morphological studies on Meckel’s diverticulum in the duck (Ansa ansa domesticus)
14
16
EN
A. A.
Mohammadpour
Department of Basic Sciences, School of Veterinary Medicine, University of Shahrekord, Shahrekord, Iran
a_mopour@yahoo.com
10.22099/ijvr.2006.2674
This research was carried out to define the morphological features of Meckel’s diverticulum (MD) in<br />duck and to investigate whether the MD resides in a constant position along the intestinal tract. For this<br />purpose, a total of 30 adult healthy ducks of both sexes, 50-52 weeks of age were used. After dissecting,<br />some morphological factors such as: total intestine length as well as the lengths of the intestine from the<br />gizzard to MD (GMD), from gizzard to cloaca (GC) and from MD to the caecal opening (MDC) were<br />measured. The weight, thickness and length of MD were then recorded. By using t-test and analysing factors<br />between two sexes we concluded that, the location of MD in total specimens was 91.52 ± 8.09 cm far away<br />from the gizzard. There was no significant difference between morphological factors of MD (weight,<br />thickness and length) in both sexes (P>0.05). There was significant difference (P<0.05) between GMD, GC<br />in both sexes. In histological studies of MD, aggregated lymphatic follicles and thin muscularis mucosa were<br />observed in tunica mucosa.
Morphological study,Meckel’s diverticulum,Duck
https://ijvr.shirazu.ac.ir/article_2674.html
https://ijvr.shirazu.ac.ir/article_2674_15d27b3c1b0d00f6cac91cbf70dd2420.pdf
Shiraz University
Iranian Journal of Veterinary Research
1728-1997
2252-0589
7
1
2006
03
01
Polymerase chain reaction for the detection and differentiation of Marek’s disease virus strains MDV-1 and HVT
17
21
EN
M. R.
Sadeghi
National Institute for Genetic Engineering and Biotechnology, Tehran, Iran
S. A.
Ghorashi
National Institute for Genetic Engineering and Biotechnology, Tehran, Iran
alig@nrcgeb.ac.ir
R.
Kargar Moakhar
Razi Vaccine and Serum Research Institute,
Karadj, Iran
D.
Morshedi
National Institute for Genetic Engineering and Biotechnology, Tehran, Iran
R.
Salehi Tabar
National Institute for Genetic Engineering and Biotechnology, Tehran, Iran
S. S.
Ghaemmaghami
Veterinary Medicine Research
Center, Agriculture Organization, Markazi Province, Arak, Iran
10.22099/ijvr.2006.2675
Marek’s disease (MD) is a lymphoproliferative disease of chickens characterized by lymphocytic<br />infiltration of various organs. The present study was an attempt to use polymerase chain reaction (PCR) to<br />optimize a rapid and reliable assay for detection of MDV genome. Detection of serotype 1 of MDV (MDV-1)<br />was confirmed by presence of a 200 bp DNA fragment as a PCR product. Differentiation of MDV-1 and<br />herpesvirus of turkeys (HVT) was also conducted using specific primers from the glycoprotein A (gA) gene<br />and a 388 bp DNA fragment was amplified from HVT genome. The specificity of the test was confirmed by<br />sequencing of PCR products. Results indicate that MDV-1 can be diagnosed in clinical samples and inoculated cell cultures which is used for virus isolation. In addition, differentiation between MDV-1 and HVT viruses was confirmed based on the size of PCR products. The test proved to be rapid and reliable and can be performed as a robust diagnostic test in veterinary diagnostic laboratories.
Marek’s disease virus,PCR,Detection
https://ijvr.shirazu.ac.ir/article_2675.html
https://ijvr.shirazu.ac.ir/article_2675_04cdb1d922cc0ba55fd73aea7a1bb43e.pdf
Shiraz University
Iranian Journal of Veterinary Research
1728-1997
2252-0589
7
1
2006
03
01
Morphological and stereotaxic studies of Iranian native goat’s brain
22
28
EN
A. A.
Mohammadpour
Department of Basic Sciences, School of Veterinary Medicine, University of Shahrekord, Shahrekord, Iran
a_mopour@yahoo.com
10.22099/ijvr.2006.2676
This study was conducted to find out the best outside points of skull to reach different areas of brain that<br />are essential for neuroendocrinological studies. In this research 30 heads of Iranian native male goats aged<br />between 2-3-year-old were collected from Shahrekord abattoir. After collecting whole heads, they were fixed<br />in 10% formalin and then many holes were made on the dorsal surface of skull followed by median and<br />transverse sectioning of the heads. Morphological characteristics of brain and distances from the bones of<br />skull to different structures of brain were measured using caliper device and needle and the best points were<br />determined. By this method the best points to reach the hypophysis and lateral ventricle with 90° angle are in distances of 45.2 ± 2.23 mm and 20.92 ± 1.02 mm at bregma point.
Stereotaxy,Goat,Brain
https://ijvr.shirazu.ac.ir/article_2676.html
https://ijvr.shirazu.ac.ir/article_2676_f51b14cb469875c909350d8f54004007.pdf
Shiraz University
Iranian Journal of Veterinary Research
1728-1997
2252-0589
7
1
2006
03
01
An experimental study on surgical wound contamination from distant infective source in rabbits
29
33
EN
F.
Sarrafzadeh-Rezaei
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Urmia, Urmia, Iran
f.sarrafzadeh@mail.urmia.ac.ir
Gh.
Yousefbaigi
Department of Pathobiology, Faculty of Veterinary Medicine, University of Urmia, Urmia, Iran
A.
Shariati
Graduated from Faculty of Veterinary Medicine, University of Urmia, Urmia, Iran
10.22099/ijvr.2006.2677
Surgical wound infections are usually encountered due to the endogenous rather than the exogenous<br />infective sources. The objective of this study was to evaluate the effect of a distant inoculated infective source on the contamination of surgical wounds. Thirty White New Zealand rabbits divided randomly into three control and three treatment equal groups. In treatment groups 24 hrs before surgical intervention<br />Staphylococcus aureus was injected subcutaneously in right thigh. In model animals, skin and muscles were<br />incised 1 cm to the right of the vertebral column and sutured, immediately. The relative frequency of<br />staphylococcal contamination of tissue specimens at 24 and 48 hrs after surgery in treatment groups were 20% and 60%, respectively. Statistical analysis did not show any significant differences in the rate of contamination between control and treatment groups at aforementioned times (P>0.05). However, comparison at 72 hrs after surgery, showed that the rate of contamination in treatment group is significantly more than control ones (P = 0.004). The presence of S. aureus in wounds of treatment animals at 72 hrs after surgery, suggested that microorganisms lodged in any part of body other than wound region could contaminate it, which could be important in wound healing.
Wound healing,Staphylococcus aureus,Rabbit
https://ijvr.shirazu.ac.ir/article_2677.html
https://ijvr.shirazu.ac.ir/article_2677_56667262cf0b799bfa4177d7a86834f8.pdf
Shiraz University
Iranian Journal of Veterinary Research
1728-1997
2252-0589
7
1
2006
03
01
Effects of oral iron supplementation on haematocrit, live weight gain and health in neonatal dairy calves
34
37
EN
M.
Mohri
Department of Clinical Sciences, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
mohri@ferdowsi.um.ac.ir
F.
Sarrafzadeh
Department of Clinical Sciences, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
H. A.
Seifi
Department of Clinical Sciences, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
10.22099/ijvr.2006.2678
Forty neonate calves were used in this study. The animals were divided into two treatment (n = 20) and<br />control (n = 20) groups. In the treatment group, oral iron as ferrous sulphate was supplemented to each calf at<br />the dose of 150 mg/day for 28 days, from the birthday. Blood sampling was taken from jugular vein<br />immediately after birth and on days 7, 14, 21 and 28 after birth. Significant differences in haematocrit (PCV)<br />levels were found between two groups on days 14, 21 and 28 (P<0.05). Total gain, mean daily gain and weight<br />gain during 4th week of life was significantly higher in the treatment group than control group (P ≤ 0.05).<br />Chi-square test proved no significant difference between the two groups regarding the incidence of diseases.
Haematocrit,Health,Iron,Neonatal dairy calves,Performance
https://ijvr.shirazu.ac.ir/article_2678.html
https://ijvr.shirazu.ac.ir/article_2678_7abbdc8486c9ac81ebcc76626de09786.pdf
Shiraz University
Iranian Journal of Veterinary Research
1728-1997
2252-0589
7
1
2006
03
01
The effect of different levels of sodium chloride on round heart disease in the turkey
38
43
EN
M.
Jahantigh
Department of Poultry Diseases, College of Veterinary Medicine, University of Zabol, Zabol, Iran
mjahantig@yahoo.com
10.22099/ijvr.2006.2679
Round heart disease is a disease of unknown etiology in turkey. The objective of this study was to examine<br />the effect of different amounts of sodium chloride in inducing cardiomyopathy in turkey poults. There are some reports that hypoxia and furazolidone can induce cardiomyopathy in turkey. Two hundred 2-day-old turkey poults were wing-banded and placed randomly into 6 groups and 24 replicates (4 replicates for each group). The 6 groups received commercial turkey starter diet contain 0.12, 0.17, 0.22, 0.27, 0.32 and 0.37% of sodium, respectively. They had access to food and water ad libitum. Suffering and dead birds were weighed and examined for post-mortem study. Live body weight of turkey poults measured individually on days 1, 2, 5 and 19. To study the hearts for presence of cardiomyopathy, on the day 19, all the poults were weighed and killed by cervical dislocation. The pieces of the hearts were placed into numbered tubes, flash frozen by liquid nitrogen and stored at -70ºC for myoglobin and hemoglobin microassay. The assay was based on spectrophotometry and separated myoglobin and hemoglobin by ammonium sulphate extraction. For determination of cardiomyopathy the ratio of the inner cavity of left ventricle to the total diameter of the left ventricle (IClv/TDlv) was calculated. Myopathic ratio for groups 1, 2, 3, 4, 5 and 6 were 0.1, 0.29, 0.3, 0.34, 0.35 and 0.36, respectively. Turkey poults which received excessive salts drank more water which produced volume overload, more cardial muscle activity, needed more oxygen and were more likely to develop myopathy. Cardiac muscle myoglobin and hemoglobin microassay showed that there was no significant relationship between the amount of myoglobin and hemoglobin with the amount of diet sodium.
Round heart disease,Sodium chloride,Myoglobin,Turkey poults
https://ijvr.shirazu.ac.ir/article_2679.html
https://ijvr.shirazu.ac.ir/article_2679_41eaaa078a37b9bef08759cc702ef6e2.pdf
Shiraz University
Iranian Journal of Veterinary Research
1728-1997
2252-0589
7
1
2006
03
01
Acquired reproductive tract abnormalities of ewes in northwest of Iran: an abattoir survey
44
48
EN
A.
Saberivand
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Urmia, Urmia, Iran
adel64ir@yahoo.com
M.
Haghighi
Graduated from Faculty of Veterinary Medicine, University of Urmia, Urmia, Iran
10.22099/ijvr.2006.2680
Reproductive abnormalities cause major losses of sheep production. Abattoir materials were used to<br />determine the extent of reproductive wastage. Reproductive tracts were collected from 524 ewes slaughtered at Urmia slaughter-house in Urmia, northwest of Iran, during a period of 12 months. Overall, 30.10% of the ewes examined were pregnant. A total of 149 (28.44%) tracts developed acquired reproductive tract abnormalities. Based on the observation of the tracts, abnormalities of the ovaries, ovarian bursa and uterine tubes and uterine lesions accounted for 11.41%, 21.48% and 67.11% of the lesions, respectively. The most common ovarian lesion was ovario-bursal adhesions (47.06%). Uterine tube obstruction was the most common uterine tube abnormality (8.07%). Pyometra (8.07%), uterine hemorrhage (6.73%) and endometritis (4.04%) were the most common abnormalities observed in the uterus. Two tracts (0.9%) contained macerated fetal remnants. A large number of tracts (16%) had melanin pigment in the uterine wall. It is concluded that, firstly, the large number of pregnant ewes slaughtered highlights significant economic losses. Secondly, lesions such as hydrosalpinx, pyometra and metritis were likely to affect female sheep reproduction. The acquired abnormalities were therefore more significant in terms of individual animal infertility than as a major cause of infertility in flocks.
abnormalities,Female reproductive tract,Ewe,Infertility
https://ijvr.shirazu.ac.ir/article_2680.html
https://ijvr.shirazu.ac.ir/article_2680_55c02657671ed1fb8ca9d08545539565.pdf
Shiraz University
Iranian Journal of Veterinary Research
1728-1997
2252-0589
7
1
2006
03
01
Seasonal variation in the characteristics of the Azarbaijani buffalo (Bubalus bubalis) semen
49
54
EN
S. M.
Alavi-Shoushtari
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Urmia, Urmia, Iran
m.alavi@mail.urmia.ac.ir
B.
abazadeh-Habashi,
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Urmia, Urmia, Iran
10.22099/ijvr.2006.2681
To study the seasonal variation in the characteristics of the Azarbaijani buffalo semen, three 2-4-year-old<br />bulls of Azarbaijani water buffaloes, kept in the Buffalo Breeding Center, in Urmia, northwest of Iran, were<br />selected. Semen samples were collected once a week for a period of one year using artificial vagina and a<br />buffalo cow as a dummy. Semen volume, colour, pH and motility and spermatozoa motility, viability,<br />morphology and concentration were examined. During one calendar year, 129 semen samples were examined. The mean values were plotted and a curve was drawn for the annual variations in each parameter. The comparison of the mean values in each sampling and in different seasons and the correlation between these variations and ecological factors, such as temperature, rainfall and day length were also studied. Semen was characterized by a mean (±SEM) ejaculation volume of 4 ± 0.14 ml, semen colour density score of 3.75 ± 0.07, pH of 6.97 ± 0.03 and motility score of 2.89 ± 0.05. The sperm motility of 75.85% ± 1.59% and sperm viability of 73.2% ± 1.56% were observed; 11.55% ± 0.42% of spermatozoa had abnoraml morphology. The mean (±SEM) sperm concentration was 1239.9 ± 34.11×106 cells/ml. All of the studied parameters showed fluctuations throughout the year but these variations were statistically significant only in some occasions and were mostly correlated with length of the day. Semen of better quality was collected in summer and autumn.
Buffalo,Semen,Seasonal variations
https://ijvr.shirazu.ac.ir/article_2681.html
https://ijvr.shirazu.ac.ir/article_2681_01f58699333bff903c618252a931003d.pdf
Shiraz University
Iranian Journal of Veterinary Research
1728-1997
2252-0589
7
1
2006
03
01
Study on the frequency of pregnant ewes slaughtered in Khorram Abad abattoir, Iran
55
58
EN
A.
Kheradmand
Department of Clinical Sciences, College of Veterinary Medicine, University of Lorestan, Khorram Abad, Iran
arashkheradmand@yahoo.com
R. A.
Batavani
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Urmia, Urmia, Iran
H.
Babaei
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
babaei@mail.uk.ac.ir
10.22099/ijvr.2006.2682
The aim of this survey was to evaluate the frequency of pregnant ewes slaughtered in Khorram Abad<br />abattoir and estimation of their gestational age. Over a period of five months in fall and winter, a total<br />number of 3046 uterine tracts were collected. Pregnancy was detected in 56.50% of ewes, 52.36% and 4.13% of which carried singleton and twins, respectively. The highest frequency of pregnancy was detected in<br />January (65.63%) and the lowest frequency was detected in March (35.02%). Among a total number of 1721<br />pregnant ewes, the most frequency of gestational age (34.68%) was recorded to be between 2 and 3 months of gestation, while 5.05% was detected to be above 121 days. Overall, the number of pregnant ewes with a gestational age over two months was considerable (59.83%). It was concluded that reproductive waste<br />resulting from slaughtering of pregnant animals is important in ewes in Khorram Abad and could be<br />prevented by an improved antemortem examination.
Ewe,Pregnant,Gestational age,Abattoir
https://ijvr.shirazu.ac.ir/article_2682.html
https://ijvr.shirazu.ac.ir/article_2682_acaf12046e58dd56ac2d78233ae27585.pdf
Shiraz University
Iranian Journal of Veterinary Research
1728-1997
2252-0589
7
1
2006
03
01
Abattoir survey of bovine pyelonephritis
59
61
EN
I.
Karimi
Department of Pathobiology, School of Veterinary Medicine, University of Shahrekord, Shahrekord, Iran
irkarimi@yahoo.com
M.
Shahgholian
Graduated from School of Veterinary Medicine, University of Shahrekord, Shahrekord, Iran
A.
Ebrahimi
Department of Pathobiology, School of Veterinary Medicine, University of Shahrekord, Shahrekord, Iran
ebrahimi@sku.ac.ir
M. R.
Mahzounieh
Department of Pathobiology, School of Veterinary Medicine, University of Shahrekord, Shahrekord, Iran
10.22099/ijvr.2006.2683
In order to investigate the prevalence and detection of etiological agents of pyelonephritis in Shahrekord<br />abattoir, 404 kidneys of slaughtered cattle (207 female and 197 male) were inspected. Kidney samples, which<br />had gross changes were obtained for histological examination. Urine and renal tissue samples were also<br />collected aseptically for microbiological examination. In histological examination pyelonephritis were<br />observed in 3 cases (0.74%), one of which occurred in a 7-year-old cow and the others in bulls less than 2-<br />year-old. In microbiological examination E. coli was isolated from urine and renal tissues in 2 cases and<br />Staphylococcus aureus isolated only from renal tissue in the other case.
Pyelonephritis,Cattle,Abattoir
https://ijvr.shirazu.ac.ir/article_2683.html
https://ijvr.shirazu.ac.ir/article_2683_39a0dd9ad22863e74d231eb2158c14d3.pdf
Shiraz University
Iranian Journal of Veterinary Research
1728-1997
2252-0589
7
1
2006
03
01
The biological effect of Sepedon (Diptera: Sciomyzidae) fly larvae living on Lymnea snails
62
65
EN
Gh. R.
Motamedi
Department of Parasitology, Razi Vaccine and Serum Research Institute, Karadj, Iran
g.motamedi@rvsri.com
A. H.
Dalimi Asl
Department of
Parasitology, Faculty of Medical Sciences, Tarbiat Moddares University of Tehran, Tehran, Iran
M. A.
Akhavizadegan
Department of Bacteriology and Medical Center, Razi Vaccine and Serum Research Institute, Karadj, Iran
R.
Pilehchian Langroodi
Department of Bacteriology and Medical Center, Razi Vaccine and Serum Research Institute, Karadj, Iran
M.
Abdigoudarzi
Department of Bacteriology and Medical Center, Razi Vaccine and Serum Research Institute, Karadj, Iran
M.
Mohammadi
Department of Bacteriology and Medical Center, Razi Vaccine and Serum Research Institute, Karadj, Iran
10.22099/ijvr.2006.2684
The present work was carried out to investigate first, the ecology of the Sepedon flies as well as species<br />of Lymnea snails and secondly, the biological effects of Sepedon fly larvae living on Lymnea. In this regard,<br />the life cycle, death rate and compatibility of Lymnea species, as well as the life cycle of Sepedon flies in<br />breeding cage and the activity of the new larvae in the same ecological condition were investigated. The<br />results showed that Lymnea truncatula needs more sophisticated ecological and feeding conditions than other species of the snails. Lymnea stagnalis was found sensitive to temperature variation and this may lead to gradual or sudden death. Lymnea pregra and Lymnea palustris could well adapt with laboratory conditions.<br />The killing effect (P<0.001) of the third-stage larvae on snails (3 to 4 weeks) was more than the effect of<br />first- and second-stages larvae. This effect was more in a container with 2 cm water depth than a container<br />with 5 cm depth.
Biological control,Sciomyzidae,Sepedon,Lymnea
https://ijvr.shirazu.ac.ir/article_2684.html
https://ijvr.shirazu.ac.ir/article_2684_fd7ec05ee49a491004864c7afe7dc5e6.pdf
Shiraz University
Iranian Journal of Veterinary Research
1728-1997
2252-0589
7
1
2006
03
01
The status of cobalt in soil, plants and sheep in Shahrekord district, Iran
66
69
EN
Gh. A.
Kojouri
0000-0002-1233-5487
Department of Clinical Sciences, School of Veterinary Medicine, University of Shahrekord, Shahrekord, Iran
drgholam_alikojouri@yahoo.com
10.22099/ijvr.2006.2685
Cobalt (Co) deficiency occurs in many parts of world especially in tropical and subtropical countries.<br />Primary Co deficiency occurs only in places where the soil is deficient in Co. The type of soil and the presence of other substances like manganese and lime influence the absorption of Co (secondary Co deficiency). To determine the Co status in Shahrekord district, Iran, several important farms were selected and the Co concentration of 80 different soils (before cultivation) under cultivation of alfalfa, clover, barley and wheat and their cultivated plants (after full vegetative growing) were measured by atomic absorption spectroscopy. Moreover, blood samples were taken from 100 sheep fed the grown plants on those areas. The mean ± SD Co concentration in the soil of alfalfa, clover, barley and wheat farms were 0.14 ± 0.04, 0.16 ± 0.06, 0.10 ± 0.03 and 0.11 ± 0.05 mg/kg, respectively and the corresponding concentrations in plants were 1.32 ± 1.01, 2.2 ± 0.08, 1.2 ± 0.76 and 1.37 ± 0.88 mg/kg dry matter (DM), respectively. The mean ± SD Co concentration in sheep serum was 1.45 ± 0.41 μg/dl at various physiological conditions. Results indicated that in all studied areas, Co concentration in soil was lower than the normal range and primary Co deficiency was noticeable. On the other hand, Co concentration in different plants and ration was higher than animal demands, but serum Co was at marginal level and probably interfered with animal health. This study showed that the soils of lands under cultivation of clover and alfalfa and its related plants contained more Co than that of determined for barley (P<0.05). Furthermore, clover soil had higher amount of Co than wheat soil (P<0.05). There was a negative correlation between the amount of lead in soil of lands under cultivation of alfalfa and the concentration of Co in plants. Such a negative correlation was also detected between the amount of molybdenum and lead in soil and the concentration of Co in plants (P<0.05). Presence of some interfering factors in ration such as nitrogen (1.24 ± 0.3%), phosphorus (0.47 ± 0.18%), lead (2.87 ± 1.58 mg/kg DM) and molybdenum (0.1 ± 0.14 mg/kg DM) were negatively affected the uptake of Co and decreased serum Co concentration via secondary Co deficiency.
Cobalt,Deficiency,Minerals,Sheep,Soil
https://ijvr.shirazu.ac.ir/article_2685.html
https://ijvr.shirazu.ac.ir/article_2685_ccdd96eecd62d64301370cc50bc451fb.pdf
Shiraz University
Iranian Journal of Veterinary Research
1728-1997
2252-0589
7
1
2006
03
01
The frequency of photosensitization in a dairy cattle herd infected by Leptospires
70
72
EN
M.
Talebkhan Garoussi
Department of Clinical Sciences, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
garoussi@ut.ac.ir
10.22099/ijvr.2006.2686
Leptospirosis is a bacterial zoonotic disease, which can induce hepatic lesions. Photosensitization (PS)<br />maybe is the result of accumulation of photodynamic agents in unpigmented area of the skin exposed to<br />sunlight which occurred due to hepatic lesions. An outbreak of leptospirosis was occurred in a dairy cattle<br />herd complex with 760 dairy milking cows in March (1990). Totally, 412 cows (54.21%) showed clinical<br />signs including fever, systemic mastitis, haemoglobinuria, jaundice, lameness, abortion and stillbirth. The<br />microscopic agglutination test (MAT) was used for diagnosis of leptospirosis. The herd was infected with<br />Leptospira interrogans grippotyphosa, L. interrogans icterohaemorrhagiae, L. interrogans pomona, L.<br />interrogans canicola and L. interrogans hardjo. Only one (0.13%) cow showed PS in the unpigmented skin<br />dorsum. MAT showed that she was infected with L. interrogans pomona, L. interrogans hardjo and L.<br />interrogans icterohaemorrhagiae with titre of 1 : 3200 of antibody. In spite of the high incidence of<br />leptospirosis, the frequency of PS was too low.
Photosensitization,Leptospirosis,Dairy cattle
https://ijvr.shirazu.ac.ir/article_2686.html
https://ijvr.shirazu.ac.ir/article_2686_533169e2bab9a17e00fda223331ad8e3.pdf