Shiraz University
Iranian Journal of Veterinary Research
1728-1997
2252-0589
7
4
2006
12
01
Elastic cartilage grafting in canine radial fracture
1
7
EN
S.
Saifzadeh
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Urmia, Urmia, Iran
s.saifzadeh@mail.urmia.ac.ir
R.
Hobbenaghi
Department of Pathobiology, Faculty of Veterinary Medicine, University of Urmia, Urmia, Iran
Sh.
Hodi
Graduated from Faculty of Veterinary Medicine, University of Urmia, Urmia, Iran
10.22099/ijvr.2006.2627
Bone has a capability to repair itself when it is fractured. Repair involves the generation of intermediate<br />tissues, such as fibrous connective tissue, cartilage and woven bone, before final bone healing can occur. The<br />process of cartilage-to-bone transition (CBT) is a key for the achievement of rigid bone healing during<br />fracture repair. We tested this potential for elastic cartilage using a long bone defect model in dog. Eight<br />sexually intact female mongrel dogs, 4.57 ± 0.53 years old and weighing 11.48 ± 0.63 kg, were studied. After<br />an ostectomy of the midshaft radius, bone healing was evaluated over an 8-week period in control dogs (n =<br />4) and dogs in which autologous grafts of auricular cartilage were inserted into the bone defects (n = 4).<br />Quantitative radiographic assessment was conducted every 2 weeks. Eight weeks post-operative, qualitative<br />histopathologic analysis was performed on the operated radii. Furthermore, histological grading was done<br />using the Ulutas et al., scoring system. Experiment dogs had more advanced radiographic healing of<br />ostectomy sites. The defects with elastic cartilage implants were bridged completely with new bony spicules<br />originated from the implants. Transformation of elastic cartilage clusters to mesenchymal connective tissue<br />and bony spicules was obvious in the experiment group. Significant differences were observed for cellular<br />morphology [3 ± 0.82 (experiment) vs. 1.75 ± 0.5 (control)] and cartilage integration [2 (experiment) vs. 1<br />(control)] at ostectomy sites between the studied groups. This study demonstrated that by using the<br />ostectomy gap model, autologous auricular cartilage enhanced the radiographic and histopathologic aspects<br />of bone healing in dogs.
Elastic cartilage,Auricular,Ostectomy,Bone healing,dog
https://ijvr.shirazu.ac.ir/article_2627.html
https://ijvr.shirazu.ac.ir/article_2627_03405ed2d0c9e63b17cb72e678ede052.pdf
Shiraz University
Iranian Journal of Veterinary Research
1728-1997
2252-0589
7
4
2006
12
01
Histopathological study of gills in experimentally amoebic gill disease (AGD) infected Atlantic salmon, Salmo salar, L.
8
13
EN
R.
Peyghan
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
peyghan_r@scu.ac.ir
M. D.
Powell
Department of Aquaculture, Tasmanian Aquaculture and Fisheries Institute, University of Tasmania, Launceston, Australia
10.22099/ijvr.2006.2628
Amoebic gill disease (AGD) is the most important parasitic disease of Atlantic salmon industry in<br />Australia. Atlantic salmon (Salmo salar) experimentally infected with Neoparamoeba sp. apparently showed<br />AGD gross signs on the gill and an amoebic-associated gill pathology. Physico-chemical factors of water<br />during the experiment were monitored regularly and were approximately constant (temperature: 17oC,<br />salinity: 35 g/l, total ammonia: 0.25 mg/l, pH = 7.9). In this study significant gill pathology was observed<br />histologically, and in all of the sections a multifocal hyperplasia and fusion of adjacent secondary lamellae<br />was seen. The severity of pathological changes observed in the sections did not always correspond with the<br />number of amoebae and even occurred in the absence of amoebae. Some histopathological changes that were seen in the secondary lamellae are: thickening of the secondary lamellae due to hyperplasia, reduction in chloride cell density and an increase in mucous cell numbers of the epithelium. Some of neighboring<br />secondary lamellae was seen attached to one another, but entire fusion of the primary lamellae was not<br />observed. Amoebae were seen in all sections in significant densities mostly in the outer part of hyperplasic<br />tissues.
Histopathology,Neoparamoeba,Atlantic salmon,Amoebic gill disease
https://ijvr.shirazu.ac.ir/article_2628.html
https://ijvr.shirazu.ac.ir/article_2628_3bbf108367f0eb658f943a2522be4dcb.pdf
Shiraz University
Iranian Journal of Veterinary Research
1728-1997
2252-0589
7
4
2006
12
01
Effect of lactose extender with different levels of osmolality and pH on the viability of Bactrian camel (Camelus bactrianus) spermatozoa
14
22
EN
A.
Niasari-Naslaji
0000-0002-0002-3339
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
niasari@ut.ac.ir
S.
Mosaferi
Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University of Tabriz,
Tabriz, Iran
N.
Bahmani
Graduated from Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
A.
Abarghani
Research Center for Agriculture and Natural Resources, Ministry of Jahad Agriculture, Ardabil, Iran
A. A.
Gharahdaghi
Animal Science Research Institute, Karadj, Iran
A.
Gerami
Department of Mathematics, Faculty of Sciences, University of Tehran, Tehran, Iran
10.22099/ijvr.2006.2629
The effect of different levels of osmolality and pH of lactose extender on the viability of Bactrian camel<br />spermatozoa was investigated. Semen was collected from 10 Bactrian camel bulls using modified bovine<br />artificial vagina. In experiment I, the extenders consisted of 9, 10, 11, 12 and 13% lactose with respective<br />osmolalities of 290, 333, 350, 376 and 419 mOsm/kg and the pH of 6.9. In experiment II, 10% lactose<br />extender with different levels of pH = 5.9, 6.9, 7.5, 7.9 and 8.9 were compared. All extenders contained 20% egg yolk and antibiotics. There were three replicates (ejaculates) for each trial. After dilution, semen was evaluated for progressive forward motility (PFM), plasma membrane integrity (PMI) and live percentage of sperm, at time 0, 4, 12 and 24 hrs after incubation at 4°C. Viability of sperm was similar among 9-11%<br />lactose concentrations at time 0 and 4. PFM was compromised (<8%) at 10 and 11% lactose extenders at<br />time 12. Although, PFM was greatest at time 0 (P<0.05) for the pH of 6.9, it reduced to 15% at time 4<br />(P<0.05). At the pH of 5.9-7.9, PMI and live percentage of sperm reduced at time 12 and 24, respectively<br />(P<0.05). In conclusion, 9-11% lactose with the pH of 6.9 may consider as a suitable extender for the shortterm preservation (up to 4 hrs) of Bactrian camel semen maintained at chilled condition (4°C).
Bactrian camel semen,Lactose,Osmolality,pH,Short preservation
https://ijvr.shirazu.ac.ir/article_2629.html
https://ijvr.shirazu.ac.ir/article_2629_7e3487cae7fdcf03d7fbedf8c5d3b1d0.pdf
Shiraz University
Iranian Journal of Veterinary Research
1728-1997
2252-0589
7
4
2006
12
01
Effect of ewe breed on the reaction time and semen characteristics in the ram
23
26
EN
A.
Kheradmand
Department of Clinical Sciences, College of Veterinary Medicine, University of Lorestan, Khorram Abad, Iran
kheradmand_a@lu.ac.ir
H.
Babaei
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of
Kerman, Kerman, Iran
10.22099/ijvr.2006.2630
The aim of this study was to determine the effect of ewe breed on the reaction time and semen<br />characteristics in the ram. For this purpose, from the native sheep breeds in Iran, seven Lory rams and four<br />ewes (two Lory and two Ghezel breeds) were selected. Seven rams were individually subjected to 4 weekly<br />trials in which one of the following two treatments was applied alternately. All of rams were scheduled twice<br />weekly to introduce with the same (Lory) and different (Ghezel) ewe breed individually. In each session, the<br />reaction time and number of mounts before ejaculation were recorded. At two another sessions per week,<br />sperm evaluation was performed in each group after collection of semen by artificial vagina. When the both<br />breeds of the ram and ewe were the same, the mean of reaction time, number of mounts, ejaculation volume, sperm concentration and sperm viability were 44.6 sec, 2.7, 0.97 ml, 4.49 × 109 sperm/ml and 88.9%, respectively and when the breeds were different, the means were 61.5 sec, 2.9, 1.0 ml, 4.52 × 109 sperm/ml and 88.4%, respectively. The reaction time was significantly lower in the first group (P<0.01). However, there was no significant difference in the other parameters between the two groups. It was concluded that during semen collection, when the both breeds of the ram and ewe are the same, the reaction time becomes significantly lower, but the number of mounts and semen characteristics (volume, sperm concentration and viability) did not differ.
Ewe breed,Lory ram,Semen characteristics
https://ijvr.shirazu.ac.ir/article_2630.html
https://ijvr.shirazu.ac.ir/article_2630_4ae432bd0ab9584d31c74c1a64921ec0.pdf
Shiraz University
Iranian Journal of Veterinary Research
1728-1997
2252-0589
7
4
2006
12
01
The effect of ovalbumin and mannose-conjugated ovalbumin on the prevention of Salmonella adherence to the intestinal epithelium of chickens
27
32
EN
T.
Karimi
Ph.D. Student in Biochemistry, School of Veterinary Medicine, University of Shiraz, Shiraz, Iran
K.
Asasi
Department of Poultry Sciences, School of Veterinary Medicine, University of Shiraz, Shiraz, Iran
asasi@shirazu.ac.ir
M.
Aminlari
Department of Biochemistry, School of Veterinary Medicine, University of Shiraz, Shiraz, Iran
T.
Vaseghi
MSc in Laboratory, Department of Biochemistry, School of Veterinary Medicine, University of Shiraz, Shiraz, Iran
10.22099/ijvr.2006.2631
This investigation was designed to determine the effect of intact ovalbumin and mannose-conjugated<br />ovalbumin on the prevention of Salmonella typhimurium adherence to the epithelium of small intestine of<br />chickens. Mannose-conjugated ovalbumin was produced by Maillard-type reaction between chicken<br />ovalbumin and D-mannose at 60°C. The results revealed that incubation up to 96 hrs caused the highest<br />amount of covalent attachment of mannose to the ovalbumin. In order to determine the effect of native<br />ovalbumin and mannose-conjugated ovalbumin on the prevention of S. typhimurium adherence to chicken<br />small intestine, 60 one-day-old chicks were randomly assigned to 3 groups, with two replicates and ten<br />birds per pen. Groups 1, 2 and 3 received normal diet, diet containing 0.5% native ovalbumin and diet<br />containing 0.5% mannose-conjugated ovalbumin, respectively, for 12 days. On day 3, all groups received<br />1.3 × 106 CFU of S. typhimurium orally. On days 4, 7 and 10, two chicks from each group were killed and<br />mean log 10 of CFU (colony forming unit) of Salmonella per 1 g tissues of cecum, liver and spleen was<br />determined. Four chickens from each group were killed on day 12 and were examined as described above.<br />The results showed that in group 3, number of viable Salmonella in cecum, liver and spleen was lower than<br />groups 1 and 2. However, the difference was significant only in cecum on days 4 and 7 (P<0.05). These<br />preliminary results suggest that mannose-conjugated ovalbumin might be effective in prevention of<br />Salmonella colonization in the epithelium of small intestine if incorporated in the diet of chicks.
Ovalbumin,Mannose,Conjugation,Intestinal mannose receptors,Salmonellosis
https://ijvr.shirazu.ac.ir/article_2631.html
https://ijvr.shirazu.ac.ir/article_2631_424983e9572b71ae30b10f130a73e02e.pdf
Shiraz University
Iranian Journal of Veterinary Research
1728-1997
2252-0589
7
4
2006
12
01
Effect of xylazine and yohimbine on the phasic pain during the estrous cycle in the rat
33
39
EN
M.
Taherianfard
Department of Physiology, School of Veterinary Medicine, University of Shiraz, Shiraz, Iran
taherian@shirazu.ac.ir
Z.
Khazaee
Graduated from School of Veterinary Medicine, University of Shiraz, Shiraz, Iran
10.22099/ijvr.2006.2632
The aim of the present study was to investigate the effect of α2-adrenergic agonist (xylazine) and<br />antagonist (yohimbine) on phasic pain during estrous cycle in female rats. Adult female rats weighing 180-<br />220 g were kept under controlled temperature (21-24°C) and light/dark conditions (light on at 6:00 a.m. and light off at 6:00 p.m.). Animals were divided into four groups: 1) control group which received 0.3 ml of<br />normal saline by intraperitoneal (IP) route; 2) IP experimental group which received 0.3 ml xylazine 3, 4.5<br />and 6 mg/kg and yohimbine 1, 2 and 4 mg/kg by IP route; 3) sham group which received 2 μl of artificial<br />cerebrospinal fluid by intra cerebral ventricle (ICV) route and 4) ICV experimental which received 2 μl<br />xylazine 10 and 20 μg/rat and yohimbine 5 and 10 μg/rat by ICV route. Cannulae were implanted into the left lateral ventricle using stereotaxic method. Pain sensitivity was measured by tail flick test, which was<br />performed before injection, 15 and 30 min after injection in all groups. Xylazine decreased pain sensitivity<br />significantly (P<0.05) during the estrous cycle; while higher analgesia was observed in the proestrus phase<br />for IP and ICV routes. Yohimbine increased pain sensitivity significantly (P<0.05) during the estrous cycle;<br />while higher hyperalgesia was observed in the metestrus phase for IP and ICV route groups. There was<br />interaction (P<0.05) between endogenous steroids and the α2-adrenergic system in the modulation of phasic pain sensitivity.
Xylazine,Yohimbine,Phasic pain,Female rat
https://ijvr.shirazu.ac.ir/article_2632.html
https://ijvr.shirazu.ac.ir/article_2632_7c0b8e4b57f06d4040472190626cc3c1.pdf
Shiraz University
Iranian Journal of Veterinary Research
1728-1997
2252-0589
7
4
2006
12
01
Comparative evaluation of the effect of antioxidants on the chilled-stored ram semen
40
45
EN
A.
Kheradmand
Department of Clinical Sciences, College of Veterinary Medicine, University of Lorestan, Khorram Abad, Iran
kheradmand_a@lu.ac.ir
H.
Babaei
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of
Kerman, Kerman, Iran
J.
Abshenas
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of
Kerman, Kerman, Iran
10.22099/ijvr.2006.2633
The aim of this investigation was to assess the effect of different antioxidants on the motility and<br />membrane integrity of the ram semen during storage at 5ºC for 0, 12, 24, 48 and 72 hrs after collection and<br />dilution. A total of 57 ejaculates from 12 Bakhtiary rams was collected by artificial vagina and were extended<br />1 : 3 with egg yolk-citrate buffer, which was containing one of the following antioxidants: BSA 1% (w/v),<br />BSA 3%, BSA 1% + sucrose 10%, BSA 3% + sucrose 10%, vitamin E (1 mg), vitamin E (2 mg), ascorbic<br />acid (0.9 mg/ml) and control group without any antioxidant. Percent of motile sperm (PMS), rate of forward<br />movement (RFM) and membrane integrity in extenders contained vitamin E groups were significantly higher<br />up to 48 hrs than other groups (P<0.05). Although, addition of 2 mg vitamin E was more effective than 1 mg<br />vitamin E in sperm protection, however, the difference was not statistically significant. Likewise, addition of<br />BSA alone or in combination with sucrose was superior to ascorbic acid in maintenance of sperm motility<br />and membrane integrity. It seems that sucrose has not positive effect on these 3 parameters in combination<br />with BSA. In conclusion, adding vitamin E to the extender of ram semen during dilution is superior in<br />maintenance of sperm kinematic parameters up to 48 hrs during storage at 5ºC.
Ram,Semen,antioxidant,Motility,Membrane integrity
https://ijvr.shirazu.ac.ir/article_2633.html
https://ijvr.shirazu.ac.ir/article_2633_22ec127561445bee24bcf7a0ccbadb93.pdf
Shiraz University
Iranian Journal of Veterinary Research
1728-1997
2252-0589
7
4
2006
12
01
Determination of copper status of grazing sheep: seasonal influence
46
52
EN
Z. I.
Khan
Department of Botany, University of Agriculture, Faisalabad, Pakistan
drzafar10@hotmail.com
A.
Hussain
Department of Botany, University of Agriculture, Faisalabad, Pakistan
M.
Ashraf
Department of Botany, University of Agriculture, Faisalabad, Pakistan
S.
Ermidou-Pollet
Department of Biological
Chemistry, University of Athens, Athens, Greece
10.22099/ijvr.2006.2634
A study was conducted to determine the copper status of different classes of grazing sheep during two<br />different seasons on a farm located in southern Punjab, Pakistan. A complete free-choice supplement was<br />available to all animals throughout the year. Soil, forage and blood plasma from animals were taken eight<br />times during the year (2005) (four times in both summer and winter seasons). Soil Cu2+ was affected by the seasonal changes (P<0.001), higher in summer than that in winter and was significantly higher than the need of plants during both seasons. While forage Cu2+ level showed non-significant seasonal (P>0.05) fluctuation in winter. It was adequate for ruminants’ requirements during both seasons. plasma Cu2+ concentrations of all classes of sheep were significantly higher in winter than that in summer showing the seasonal changes (P<0.001). Higher plasma Cu2+ was found in male sheep than lactating and non-lactating sheep during both seasons. The low Cu2+ in plasma in lactating sheep may have been due to its secretion in milk. In winter forage Cu2+ contributed in enhancing the plasma Cu2+ levels, but in summer the forage Cu2+ level, although very high, was ineffective in elevating plasma Cu2+ levels in all classes of sheep. Based primarily on plasma analyses it was concluded that although, the adequate level of plasma Cu2+ was found, it was on borderline deficient levels. Thus supplementation is needed with mixture containing Cu2+. The plus copper should be continuously supplemented to grazing animals in this semiarid region of Pakistan.
Seasonal variation,Soil,Plant,Animal,Pakistan
https://ijvr.shirazu.ac.ir/article_2634.html
https://ijvr.shirazu.ac.ir/article_2634_b724ba15291b18438c2f4fb549adb699.pdf
Shiraz University
Iranian Journal of Veterinary Research
1728-1997
2252-0589
7
4
2006
12
01
The prevalence of Aeromonas hydrophila-induced diarrhoea in the pig, buffalo and human in Pune area, India
53
58
EN
E.
Rahimi-Larki
Department of Production, Razi Vaccine and Serum Research Institute, Shiraz, Iran
rahimilarki@yahoo.com
S. S.
Nene
Department of Microbiology, School of Medicine, University of Pune, Pune, India
10.22099/ijvr.2006.2635
Aeromonas hydrophila is pathogen for several vertebrates. The bacteriological, clinical and epidemiological evidences for the role of A. hydrophila have been described in human infections. The presence of this pathogen in contaminated water is well-established and ingestion of such water may cause infection. There are many reports of acute diarrhoea associated with A. hydrophila transmitted by animals. In this study, 100 faecal samples of patients suffering from diarrhea and 33 faecal specimen from healthy individuals who served as control, were examined for presence of A. hydrophila. The faeces of pigs and buffaloes and the drinking water in this area were also examined for isolation and characterization of the bacteria. The results showed that in this area, the role of A. hydophila in development of acute human diarrhoea (1%) was less significant. The organism was sensitive to erythromycin, chloramphenicol, kanamycin, and gentamycin, but resistant to penicillin and ampicillin. A. hydrophila was present in faeces of buffaloes. Five samples of contaminated water were found toxigenic, too.
Aeromonas hydrophila,Diarrhoea,Pune,India
https://ijvr.shirazu.ac.ir/article_2635.html
https://ijvr.shirazu.ac.ir/article_2635_b00f147221861792c6b58e3098c69c6b.pdf
Shiraz University
Iranian Journal of Veterinary Research
1728-1997
2252-0589
7
4
2006
12
01
Evaluation of topical application of honey in prevention of post-operative peritoneal adhesion formation in dogs
59
62
EN
F.
Shokouhi Sabet Jalali
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Urmia, Urmia, Iran
f.shokouhi@mail.urmia.ac.ir
A. A.
Farshid
Department of Pathobiology, Faculty of Veterinary Medicine, University of Urmia, Urmia, Iran
S.
Saifzadeh
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Urmia, Urmia, Iran
s.saifzadeh@mail.urmia.ac.ir
S.
Javanmardi
Graduated from Faculty of Veterinary Medicine, University of Urmia, Urmia, Iran
10.22099/ijvr.2006.2636
The objective of this study was to determine the effectiveness of topical administration of honey in prevention of or reduction in post-surgical peritoneal adhesions in dog. The study was conducted on 18 adult<br />female mixed-breed dogs with a mean ± SD weight of 20 ± 4.25 kg. A standardized surgical trauma (serosal<br />trauma model) was made in the dogs’ descending colon to induce adhesion formation. The control group (n = 9) received saline treatment. In the experiment group (n = 9), the lesions were covered with honey. Three<br />weeks after laparotomy, the dogs were sacrificed and the adhesions were graded according to the Evans’s<br />scoring system. Overall, 67% (n = 6) of the control and 22% (n = 2) of the experiment animals presented<br />with moderate (grade-2) and severe (grade-3) adhesions. In the control group, there were 3 (33%) dogs with grade-0 or grade-1 adhesions, while in the experiment group there were 7 (78%) dogs with the same grades of adhesions. The difference between the two groups was significant (P<0.05). It was concluded that honey is a useful covering for damaged peritoneal surfaces and has high effectiveness in reducing post-operative adhesions.
Honey,Intra-abdominal adhesion,Topical,dog
https://ijvr.shirazu.ac.ir/article_2636.html
https://ijvr.shirazu.ac.ir/article_2636_800bba4aa2796663fa58ef445cf9e45e.pdf
Shiraz University
Iranian Journal of Veterinary Research
1728-1997
2252-0589
7
4
2006
12
01
The immediate enzymatic cocktail effect on the absorptive function of neobladder made by ileocystoplasty
63
67
EN
H. R.
Fattahian
Department of Surgery, Faculty of Specialized Veterinary Science, Islamic Azad University, Science and
Research Branch, Tehran, Iran
hrfattahian@yahoo.com
J.
Bakhtiari
Department of Clinical Sciences, Faculty of Veterinary Medicine, University
of Tehran, Tehran, Iran
bakhtiar@ut.ac.ir
A. M.
Kajbafzadeh
Department of Pediatric Urology, Children’s Hospital Medical Center, University
of Medical Sciences of Tehran, Tehran, Iran
I.
Nowrouzian
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
10.22099/ijvr.2006.2637
The effectiveness of an enzymatic cocktail, collagenase and trypsin, on the absorptive function of ileal<br />segment was studied to evaluate the changes within groups after performing ileocystoplasty in an animal<br />model. Twenty-one female mixed-bred Persian dogs were randomized into 7 groups. The groups included: 1) negative control group (NCG) in which no ileocystoplasty was performed (n = 3); 2) positive control group<br />(PCG) in which routine ileocystoplasty was performed (n = 3) and groups 3 to 7) those underwent ileocystoplasty with the ileal segment being treated with collagenase and trypsin for 5, 10, 15, 20 or 25 min<br />(groups, 5 minutes enzymatic treatment group (5METG), 10METG, 15METG, 20METG and 25METG; each<br />containing three dogs). To perform the glucose absorption test, 50% dextrose was instilled into the bladder<br />five weeks after surgery. The blood glucose level (BGL) was measured in each group before the study (T1),<br />two weeks after the surgery in PCG and other treatment groups (T2), before anaesthesia (T3), after<br />anaesthesia at 5-minute intervals up to 25 min (T4 to T9, respectively). There was no significant difference in BGL in T1 to T3 and T4 to T9 in 25METG and NCG, however, BGL was found significantly higher in T1 to<br />T3 and T4 to T9 in PCG, 5METG, 10METG, 15METG and 20METG. It can be concluded that collagenase<br />and trypsin can affect absorptive function of the neobladder and are able to reduce the absorptive function,<br />particularly in early days after the surgery. Moreover, these results also confirm that 50% dextrose instilled<br />into the neobladder and native urinary bladder will not increase BGL in 25METG and NCG even with<br />increasing the time of exposure of neo- or native urinary bladder to enzymatic cocktail till 25 min.
Neobladder,Absorptive function,Glucose,dog
https://ijvr.shirazu.ac.ir/article_2637.html
https://ijvr.shirazu.ac.ir/article_2637_5e2fbbfb1cbec48a363ebd64898fe5cd.pdf
Shiraz University
Iranian Journal of Veterinary Research
1728-1997
2252-0589
7
4
2006
12
01
Histochemical study of estradiol valerate-induced polycystic ovary syndrome in the rat
68
76
EN
V.
Najati
Graduated from Faculty of Veterinary Medicine, University of Urmia, Urmia, Iran
v.najati@urmia.ac.ir
R.
Sadrkhanlou
Department of Basic
Sciences, Faculty of Veterinary Medicine, University of Urmia, Urmia, Iran
S.
Hasanzadeh
Department of Basic
Sciences, Faculty of Veterinary Medicine, University of Urmia, Urmia, Iran
10.22099/ijvr.2006.2638
For the present study, and follow-up of histochemical changes in estradiol valerate-induced polycystic<br />ovary syndrome (PCOS), 48 virgin 90-day-old female rats were used. The rats were divided into the<br />treatment and control groups. For each rat of the treatment group, 4 mg estradiol valerate was injected<br />through intramuscular route for the induction of PCOS. The control group was taken the same amount of<br />sterile sesame oil. After 63 days of hormone administration, all rats were sacrificed and their ovaries were<br />collected immediately and processed for histochemical studies. These studies were consisted of localization<br />of carbohydrate using PAS method, saturated and unsaturated lipids using Oil-Red-O and Sudan Black B,<br />lipase and alkaline phosphatase. The PAS reaction was seen in some structures of the atretic and cystic<br />follicles such as on zona pellucida (ZP), basement membrane of granulosa cells, connective tissue fibers of<br />ovarian stroma, follicular fluid and granulose cells. This reaction was enhanced in basement membrane of<br />granulose cells of cystic follicles. The lipid droplets were seen in follicular structures of both the atretic and<br />cystic follicles. The presence of macrophages was confirmed in cystic ovaries by this study. The lipase<br />reactions were seen in granulose and theca cells of atretic and cystic follicles. The lipase reaction in the theca<br />layer of cystic follicles was stronger than in the granulose cell layer of such follicles. The alkaline<br />phosphatase reaction was seen in the theca and granulose cells of atretic and cystic follicles and by the<br />progression of these processes and cystic follicle formation, the reaction was increased accordingly. We<br />concluded that, during follicular atresia and cystic follicle formation, histochemical alterations are occurred<br />in follicular structures.
Polycystic ovaries,Estradiol valerate,Histochemistry,Rat
https://ijvr.shirazu.ac.ir/article_2638.html
https://ijvr.shirazu.ac.ir/article_2638_e96750f71ab28e68cfc66e71ff3d3fb5.pdf
Shiraz University
Iranian Journal of Veterinary Research
1728-1997
2252-0589
7
4
2006
12
01
A study on Leishmania infection rate among Phlebotomus spp. collected from Abardejh district, Iran
77
81
EN
H.
Nekouie
Department of Parasitology, Pasteur Institute of Iran, Tehran, Iran
office@pasteur.ac.ir
M.
Assmar
Department of Parasitology, Pasteur Institute of Iran, Tehran, Iran
M. R.
Razavi
Department of Parasitology, Pasteur Institute of Iran, Tehran, Iran
S. R.
Naddaf
Department of Parasitology, Pasteur Institute of Iran, Tehran, Iran
10.22099/ijvr.2006.2639
Cutaneous leishmaniasis is a prevalent tropical parasitic disease in the Old World. The causative agents<br />are Leishmanial parasites, which cause various forms of cutaneous leishmaniasis. The infection is commonly<br />limited in immuno-competent individuals, but it can progress to a chronic and ulcerative disease in immunocompromised patients. The reservoirs are dogs and rodents and the vectors are different species of sandflies. In the present study, we investigated the prevalence rate of Leishmania infection among Phlebotomus mosquitoes collected from Abardejh district, Iran. Abardejh is located next to Varamin city in southeast of Tehran having a tropical ecosystem at its eastern border. Tamarisk trees and rodents have provided a suitable condition for sandfly activity. The sandflies were collected by funnel trap from rodent burrows and transferred to the Department of Parasitology, Pasteur Institute of Iran. The sampling was carried out during spring and summer (2002) with ten-day intervals. The collected sandflies were identified using discriminative morphologic features before parasitological culture on NNN medium. Analyses of the data revealed a high prevalence rate of infection among the sandflies in this region (P<0.01). The maximum activities of Phlebotomus were in the months of June and July. Three species of sanflies were found in rodent burrow: P. papatasi, P. sergenti, and P. caucasicus. The results of blood-fed Phlebotomus culture showed that 22.07% of blood-fed females of P. papatasi and 8% of blood-fed females of P. sergenti were infected with leptomonads (P<0.05). This could be an important issue because human and agricultural environments are located closely to this district. Therefore, use of insecticides and environmental sanitation seems to be required to prevent the transmission of infection from sandflies to human.
Leishmania,Phlebotomus,Iran
https://ijvr.shirazu.ac.ir/article_2639.html
https://ijvr.shirazu.ac.ir/article_2639_1d8a956c4c7471815113bf1cb5df561c.pdf
Shiraz University
Iranian Journal of Veterinary Research
1728-1997
2252-0589
7
4
2006
12
01
Mesenteric lymph nodes infection with Linguatula serrata nymphs in cattle
82
85
EN
H.
Tajik
Department of Food Hygiene and Public Health, Faculty of Veterinary Medicine, University of Urmia, Urmia, Iran
h.tajik@mail.urmia.ac.ir
M.
Tavassoli
Department of Pathobiology, Faculty of Veterinary Medicine, University of Urmia, Urmia, Iran
m.tavassoli@urmia.ac.ir
B.
Dalirnaghadeh
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Urmia, Urmia, Iran
M.
Danehloipour
Graduated from School of Veterinary Medicine, Islamic Azad University of Urmia, Urmia, Iran
10.22099/ijvr.2006.2640
871 mesenteric lymph nodes taken from 110 native cattle from Urmia slaughter-house with different<br />ages were examined for Linguatula serrata infection. The lymph nodes were studied based on their colour<br />and consistency. 44% of the cattle were found positive for L. serrata infection. The number of parasites<br />isolated from each infected lymph node varied from 1 to 69 with a mean of 5.48. There were no significant<br />differences in the number of parasites in different age groups. The study underlined the need for a more<br />thorough investigation of mesenteric lymph nodes inspection in the cattle of this region.
Mesenteric lymph nodes,Cattle,Linguatula serrata,Pentastomida
https://ijvr.shirazu.ac.ir/article_2640.html
https://ijvr.shirazu.ac.ir/article_2640_182bc4e4031c86cdb8f8c21db3eea20e.pdf