ORIGINAL_ARTICLE
Clinical and laboratorial description of the differential diagnoses of hemostatic disorders in the horse
The process of fibrin clot formation is a series of complex and well-regulated reactions involving blood vessels, platelets, procoagulant plasma proteins, natural inhibitors, and fibrinolytic enzymes. Vasculitis can be caused by a variety of different agents as bacteria, viruses, protozoal, rickettsial organisms, toxic, drugs, medications, and neoplasms. The most common cause of vasculitis is the purpura hemorrhagica, which is associated with exposure to Streptococcus equi ssp. equi or less commonly, equine influenza. Deficiencies or defects of the hemostatic components may result in bleeding and/or thrombosis. Inherited alterations of primary hemostasis (von Willebrand disease: vWD and Glanzmann’s thrombasthenia: GT) and of secondary hemostasis (hemophilia A and prekallikrein: PK deficiency) are scarcely reported in equine clinic. On the contrary, acquired alterations of primary and secondary hemostasis are commonly found. They include thrombocytopenia, platelet dysfunction due to the administration of some drugs and targeted antiplatelet agents, decreased factor synthesis (liver disease or deficiency of vitamin K), release of inactive factors, inhibition of factor activity, or excessive consumption and depletion of factors (platelets, coagulation factors, and anticoagulants factors as antithrombin (AT) and protein C). Disseminated intravascular coagulation (DIC) is the most common and complex hemostatic disorder in horses and appears to be associated with sepsis, inflammatory and ischemic gastrointestinal tract disorders and other systemic severe diseases. These alterations are commonly found in patients in intensive care units.
https://ijvr.shirazu.ac.ir/article_5399_3cc0ca08dda95c802dd9ae406f6b464c.pdf
2020-03-01
1
8
10.22099/ijvr.2019.31826.4784
: Hemostatic disorders
horse
K.
Satué
ksatue@uch.ceu.es
1
Department of Animal Medicine and Surgery, Faculty of Veterinary, CEU-Cardenal Herrera University, Valencia, Spain
LEAD_AUTHOR
J. C.
Gardon
2
Department of Experimental Sciences and Mathematics, Catholic University of Valencia “San Vicente Mártir”, Valencia, Spain
LEAD_AUTHOR
A.
Muñoz
3
Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, University of Córdoba, Córdoba, Spain
LEAD_AUTHOR
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79
ORIGINAL_ARTICLE
The effect of date versus sugar on sensory, physicochemical, and antioxidant properties of ice cream
Background: Despite the popularity of ice cream, its high content of sugar and fat is worrisome. Substituting sugar with natural and useful resources is one possible solution. Dates (Phoenix dactylifera) are rich in carbohydrates and are a good source of energy; they also have a large amount of phenolic compounds and anthocyanin. Aims: The aim of this study is to evaluate the effect of ice cream sugar replacement with date products. Methods: Accordingly, four ice cream samples with different sweeteners including 1) sucrose (as a control), 2) date pulp + sucrose, 3) date liquid sugar, and 4) sucrose + date liquid sugar, were produced. Total phenolics, anthocyanins, and antioxidant activities of dates and their effects on physicochemical and sensory properties of ice cream were investigated. Results: A large amount of phenolic compounds and anthocyanin was found in the ice cream contained date pulp and liquid sugar, which enhanced the reducing power and antioxidant effect of it. The overrun and density of an ice cream did not change with sugar replacement. Total substitution of sucrose by date liquid sugar, increased the titratable acidity, viscosity, and antioxidant properties of ice cream, but it was found to have some negative effects on the organoleptically score of color. However, the partial replacement of granular sugar with date pulp/liquid sugar, improved the antioxidant value of this dairy dessert without any change in its color. Conclusion: It seems that the addition of date pulp to an ice cream, not only maintain the quality and sensory properties of an ice cream, but also provides an antioxidant property.
https://ijvr.shirazu.ac.ir/article_5404_a9922b441805362ea3556314121b448a.pdf
2020-03-01
9
14
10.22099/ijvr.2019.32463.4861
Antioxidants
Dates
ice cream
sugar
H. R.
Gheisari
1
Department of Food Hygiene and Public Health, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
AUTHOR
S.
Heydari
2
Graduated from School of Veterinary Medicine, Shiraz University, Shiraz, Iran
AUTHOR
S.
Basiri
basiri@shirazu.ac.ir
3
Department of Food Hygiene and Public Health, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
LEAD_AUTHOR
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31
ORIGINAL_ARTICLE
Identification of Mycoplasma ovipneumoniae and Mycoplasma arginini in sheep with pneumonia in North East of Iran
Background: Pneumonia due to Mycoplasma infections can cause serious health problems and economic losses in small ruminants industry. Aims: The aim of this study was isolation and identification of Mycoplasmas in sheep naturally infected with pneumonia in Northeastern Iran. Methods: This study used histopathology, culture and polymerase chain reaction (PCR) to examine samples from 50 lungs of sheep naturally infected with Mycoplasmas. Results: Grossly, irregular consolidation with lobular or lobar to diffuse pattern in the cranioventral to caudal lobes of affected lungs were observed. Histopathologically, bronchointerstitial pneumonia in 38 (76%), and purulent to fibrinopurulent bronchopneumonia in 12 (24%) affected sheep were diagnosed. DNA was extracted from lung tissue samples and replicated using genus and species specific primers for Mycoplasma. Mycoplasma growth was observed in 3 (6%) of a total of 50 lung samples. Genus-specific Mycoplasma DNA was identified by PCR in 12 (24%) of samples. Two (4%) and 7 (14%) samples of these 12 cases were positive for reaction with species-specific primers of Mycoplasma ovipneumoniae and Mycoplasma arginini, respectively. Conclusion: Our results showed that M. ovipneumoniae and M. arginini were the two agents that can be involved in inducing lung consolidation and pneumonia in sheep and PCR was more successful than the culture in detecting Mycoplasmas.
https://ijvr.shirazu.ac.ir/article_5436_f5e881f3f97039b21e9bbea2350fef04.pdf
2020-03-01
15
19
10.22099/ijvr.2019.34033.5036
Culture
Mycoplasma
PCR
Pneumonia
Sheep
A. A.
Daee
1
Ph.D. Student in Veterinary Pathology, Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
AUTHOR
A.
Khodakaram-Tafti
tafti@shirazu.ac.ir
2
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
LEAD_AUTHOR
A.
Derakhshandeh
3
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
AUTHOR
M.
Seyedin
4
Division of Bacteriology, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Mashhad, Iran
AUTHOR
Abtin, AR; Pourbakhsh, SA; Ashtari, A; Bayatzadeh, MA; Barani, SM and Ahangaran, S (2013). Isolation and identification of Mycoplasma agalactiae by culture and polymerase chain reaction (PCR) from sheep of Qom province, Iran. Arch. Razi Inst., 68: 11-16.
1
Adehan, RK; Ajuwape, ATP; Adetosoye, AI and Alaka, OO (2006). Characterization of Mycoplasmas isolated from pneumonic lungs of sheep and goats. Small Rum. Res., 63: 44-49.
2
Amores, J; Corrales, JC; Martin, AG; Sanchez, A and Conteras, A (2010). Comparison of culture and PCR to detect Mycoplasma agalactiae and Mycoplasma mycoides subsp. capri in ear swabs taken from goats. Vet. Microbiol., 140: 105-108.
3
Ayling, RD; Bashiruddin, SE and Nicholas, RAJ (2004). Mycoplasma species and related organisms isolated from ruminants in Britain between 1999 and 2000. Vet. Rec., 155: 413-416.
4
Ayling, RD and Nicholas, RAJ (2007). Mycoplasma respiratory infections. In: Aitken, ID (Ed.), Diseases of sheep. (4th Edn.), Oxford, UK, Blackwell Publishing Inc., PP: 231-235.
5
Azizi, S; Tajbakhsh, E; Rezaii, SH; Nekouei, SH and Namjoo, AR (2011). The role of Mycoplasma ovipneumoniae and Mycoplasma arginini in pneumonic lungs of slaughtered sheep. Rev. Med. Vet-Toulouse. 162: 310-315.
6
Carmichael, LE; St. George, TD; Sullivan, ND and Horsfall, N (1992). Isolation, propagation and characterization studies of an ovine Mycoplasma responsible for proliferative interstitial pneumonia. Cornell Vet., 62: 654-679.
7
Chazel, M; Tardy, F; Grand, DL; Calavas, D and Poumarat, F (2010). Mycoplasmoses of ruminants in France: recent data from the national surveillance network. BMC Vet. Res., 6: 32-39.
8
Cremonesi, P; Vimercati, C; Pisoni, G; Perez, G; Miranda Ribera, A; Castiglioni, B; Luzzana, M; Ruffo, G and Moroni, P (2007). Development of DNA extraction and PCR amplification protocols for detection of Mycoplasma bovis directly from milk samples. Vet. Res. Communi., (Suppl. 1), 31: 225-227.
9
Elfaki, MG; Abbas, B; Mahmoud, OM and Kleven, SH (2002). Isolation and characterization of Mycoplasma arginini from camels (Camelus dromedarius) with pneumonia. Comp. Immunol. Microbiol. Infect. Dis., 25: 49-57.
10
Ettorre, C; Sacchini, F; Scacchia, M and Salda, LD (2007). Pneumonia of lambs in the Abruzzo region of Italy: anatomopathological and histopathological studies and localization of Mycoplasma ovipneumoniae. Vet. Ital., 43: 149-155.
11
Fernandez, S; Galapero, J; Rey, J; Perez, CJ; Ramos, A; Rosales, R; Ayling, R; Alonso, JM and Gomes, L (2016) Investigations into the seasonal presence of Mycoplasma species in fattening lambs. Vet. J., 212: 80-82.
12
Gagea, MI; Bateman, KG; Shanahan, RA; van Druemel, T; McEwen, BJ; Carman, S; Archambault, M and Caswell, JL (2006). Naturally occurring Mycoplasma bovis-associated pneumonia and polyarthritis in feedlot calves. J. Vet. Diagn. Invest., 18: 29-40.
13
Goncalves, R; Mariano, I; Núñez, A; Branco, S; Fairfoul, G and Nicholas, R (2010). Atypical non-progressive pneumonia in goats. Vet. J., 183: 219-221.
14
Gutierrez, C; Rodriguez, JL; Montoya, JA and Fernandez, A (1999). Clinicopathological and haematological findings in goat kids experimentally infected simultaneously with Mycoplasma mycoides subsp. capri and Mycoplasma mycoides subsp. mycoides (large colony-type). Small Rum. Res., 31: 187-192.
15
Haziroglu, R; Diker, KS; Gulbahar, MY; Akan, M and Guvenc, T (1994). Studies of the pathology and microbiology of pneumonic lungs of lambs. Dtsch. Tierarztl. Wochenschr., 101: 441-443.
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Kheirkhah, B; Pourbakhsh, SA; Nadalian, MG; Banani, M and Ashtari, A (2011). Detection of Mycoplasma agalactiae by culture and polymerase chain reaction (PCR) methods from Iranian goats. Afr. J. Microbiol. Res., 5: 1668-1672.
17
Kılıc, A; Kalender, H; Eroksuz, H; Muz, A and Tasdemir, B (2013). Identification by culture, PCR, and immuno-histochemistry of Mycoplasmas and their molecular typing in sheep and lamb lungs with pneumonia in Eastern Turkey. Trop. Anim. Health Prod., 45: 1525-1531. doi: 10.1007/s11250-013-0394-3.
18
Lopez, A and Martinson, SA (2017). Respiratory system, mediastinum and pleura. In: Zachery, JF (Ed.), Pathologic basis of veterinary disease. (6th Edn.), Vol. 1, Saint louis, Missouri, Elsevier Inc., PP: 538-539.
19
McAuliffe, L; Hatchell, FM; Ayling, RD; King, AI and Nicholas, RA (2003). Detection of Mycoplasma ovipneumoniae in Pasteurella-vaccinated sheep flocks with respiratory disease in England. Vet. Rec., 153: 687-688.
20
Mohan, K; Obowolo, MJ and Hill, PWG (1992). Mycoplasma ovipneumoniae infection in Zimbabwe goats and sheep. J. Comp. Pathol., 107: 73-79.
21
Niang, M; Rosenbusch, RF; Lopez-Virella, J and Kaeberle, ML (1999). Differential serologic response to Mycoplasma ovipneumoniae and Mycoplasma arginini in lambs affected with chronic respiratory disease. J. Vet. Diagn. Invest., 11: 34-40.
22
Pooladgar, AR; Looni, R; Ghaemmaghami, SH; Pourbakhsh, SA; Ashtari, A and Shirudi, A (2015). Isolation and identification of Mycoplasma agalactiae by culture and polymerase chain reaction (PCR) from affected sheep to contagious agalactia of Khuzestan province, Iran. Archives of Razi Institute. 70: 21-27.
23
Radostits, OM; Gay, CC; Hinchcliff, KW and Constable, PD (2007). Veterinary medicine. A textbook of the disease of cattle, horses, sheep, pigs and goats. 10th Edn., Baillier Tindall, London, Saunders Elsevier. PP: 950-995.
24
Smith, BP; Van Metre, DC and Pusterla, N (2019). Large animal internal medicine. 6th Edn., United States, Mosby Elsevier. PP: 645-698.
25
Tola, S; Angioi, A; Rocchigiani, G; Idini, D; Manunta, D; Galleri, G and Leori, G (1997). Detection of Mycoplasma agalactiae in sheep milk samples by polymerase chain reaction. Vet. Microbiol., 54: 17-22.
26
ORIGINAL_ARTICLE
Development of a rapid, one-step-visual method to detect Salmonella based on IC-LAMP method
Background: Salmonella can cause serious human gastroenteritis and is frequently isolated from various food samples. The cell culturing, immunoassay, and polymerase chain reactions (PCR) are the current methods to detect such pathogenic agents. However, these methods are time-consuming and labor-intensive, and thus unavailable for rapid-monitoring of Salmonella. Aims: This study aimed to develop an immunocapture-loop-mediated isothermal amplification (IC-LAMP) for rapid and sensitive detection of Salmonella. Methods: Salmonella was used as antigen to produce monoclonal antibody (mAb) and mAbs were prepared via subcloning three times. The mAb 1B12 with high affinity was coated on the surface of the immuno-magnetic beads (IMBs) to capture Salmonella. The enriched products (IMBs-Salmonella) were used for LAMP using the special primers targeted the conserved invA gene of Salmonella. Results: The IC-LAMP was developed based on mAb 1B12 and LAMP. Targeting the conserved invA gene of Salmonella, the detection time was shortened to 50 min from three days. If the reaction contains Salmonella, the green fluorescence and the trapezoidal strip can be clearly observed. Importantly, the method combines the specificity of antibody and LAMP with a detection limit of 5 CFU/ml in artificially contaminated water and milk. The specificity of this method was demonstrated by testing other similar bacteria. The results indicate that the IC-LAMP reacts only with Salmonella and does not cross-react with other similar bacteria. Conclusion: The IC-LAMP assay developed here is a rapid, sensitive, one-step-visual method to screen for the presence of Salmonella in food samples. This method is faster than traditional PCR, LAMP, and other methods, and can be used as a primary screening method for the detection.
https://ijvr.shirazu.ac.ir/article_5402_c304361d1f6d6aa7ec34a024c6e4d960.pdf
2020-03-01
20
25
10.22099/ijvr.2019.29892.4521
Detection
LAMP
Magnetic Immunocapture
Monoclonal antibody
Salmonella
L.
Zhang
1
MSc Student in Biochemical Engineering, Molecular Medicine Research Center of Yunnan Province, Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China
AUTHOR
X.
Du
2
MSc Student in Biochemical Engineering, Molecular Medicine Research Center of Yunnan Province, Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China
AUTHOR
C.
Chen
3
MSc Student in Biochemical Engineering, Molecular Medicine Research Center of Yunnan Province, Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China
AUTHOR
Q.
Han
4
Molecular Medicine Research Center of Yunnan Province, Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China
AUTHOR
Q.
Chen
5
Molecular Medicine Research Center of Yunnan Province, Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China
AUTHOR
M.
Zhang
6
Molecular Medicine Research Center of Yunnan Province, Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China
AUTHOR
X.
Xia
7
Molecular Medicine Research Center of Yunnan Province, Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China
AUTHOR
Y.
Song
8
Molecular Medicine Research Center of Yunnan Province, Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China
AUTHOR
J.
Zhang
jyzhang@kust.edu.cn
9
Molecular Medicine Research Center of Yunnan Province, Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China
LEAD_AUTHOR
Alves, J; Niguma, NH and de Oliveira, TCRM (2015). Detection of Salmonella spp. in eight complex food matrices using polymerase chain reaction assay. J. Food Safety. 35: 453-457.
1
Chin, WH; Sun, Y; Hogberg, J; Quyen, TL; Engelsmann, P; Wolff, A and Bang, DD (2017). Direct PCR - A rapid method for multiplexed detection of different serotypes of Salmonella in enriched pork meat samples. Mol. Cell. Probes., 32: 24-32.
2
Datta, S; Dandapat, P and Jas, R (2018). Diagnosis of mixed gastrointestinal nematode infection in goat by an indirect-ELISA. Iran. J. Vet. Res., 19: 189-193.
3
Deb, R; Sengar, GS; Singh, U; Kumar, S; Raja, TV; Alex, R; Alyethodi, RR and Prakash, B (2017). LAMP assay for rapid diagnosis of cow DNA in goat milk and meat samples. Iran. J. Vet. Res., 18: 134-137.
4
Du, W; Wang, Y; Huang, L; Wei, Y; Chen, D; Sun, J; Wu, H; Feng, L and Liu, C (2016). Characterization of monoclonal antibodies that recognize the amino- and carboxy-terminal epitopes of the pseudorabies virus UL42 protein. Appl. Microbiol. Biotechnol., 100: 181-192.
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Elzein, M; Alum, A and Abbaszadegan, M (2013). Biochemical signature assay for use in a biosensor platform to detect bacteria in drinking water biofilms. Environ. Lett., 48: 925-932.
6
Encheng, S; Jing, Z; Tao, Y; Qingyuan, X; Yongli, Q; Wenshi, W; Peng, W; Liang, S; Jing, S and Donglai, W (2013). Analysis of murine B-cell epitopes on Eastern equine encephalitis virus glycoprotein E2. Appl. Microbiol. Biotechnol., 97: 6359-6372.
7
Handley, JA; Shi, Z; Si, HP; Dawoud, TM; Kwon, YM and Ricke, SC (2015). Salmonella and the potential role for methods to develop microbial process indicators on chicken carcasses. Food safety., PP: 81-104.
8
Hapuarachchi, CT; Jeffery, KJM and Bowler, ICJW (2019). Stool PCR may not be a substitute for enrichment culture for the detection of Salmonella. J. Med. Microbiol., 68: 395-397.
9
Hemmatzadeh, F and Kazemimanesh, M (2017). Detection of specific antigens of Newcastle disease virus using an absorbed western blotting method. Iran. J. Vet. Res., 18: 92-96.
10
Kanitkar, YH; Stedtfeld, RD; Hatzinger, PB; Hashsham, SA and Cupples, AM (2017). Development and application of a rapid, user-friendly, and inexpensive method to detect Dehalococcoides sp. reductive dehalogenase genes from ground water. Appl. Microbiol. Biotechnol., 101: 4827-4835.
11
Karp, BE; Campbell, D; Chen, JC; Folster, JP and Friedman, CR (2018). Plasmid-mediated quinolone resistance in human non-typhoidal Salmonella infections: an emerging public health problem in the United States. Zoonoses. Public. Hlth., 65: 838-849.
12
Khan, RSA; Ali, W; Kiran, S; Shah, MSD; Tahir, ZA and Habib, M (2018). Rapid detection of infectious bursal disease by loop-mediated isothermal amplification for field analysis. Iran. J. Vet. Res., 19: 101-107.
13
Law, JW; Ab Mutalib, NS; Chan, KG and Lee, LHM (2014). Rapid methods for the detection of foodborne bacterial pathogens: principles, applications, advantages and limitations. Front. Microbiol., 5: 770.
14
Liu, S; Geng, Y; Liu, L; Sun, X; Shao, J; Han, B; Wang, J and Tan, K (2018). Development of an isothermal amplification-based assay for the rapid detection of Cronobacter spp. J. Dairy Sci., 101: 4914-4922.
15
Majowicz, SE; Musto, J; Scallan, E; Angulo, FJ; Kirk, M; O’Brien, SJ; Jones, TF; Fazil, A and Hoekstra, RM (2010). The global burden of nontyphoidal Salmonella gastroenteritis. Clin. Infect. Dis., 50: 882-889.
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Mori, Y; Nagamine, K; Tomita, N and Notomi, T (2001). Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation. Biochem. Biophys. Res. Commun., 289: 150-154.
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Mori, Y and Notomi, T (2009). Loop-mediated isothermal amplification (LAMP): a rapid, accurate, and cost-effective diagnostic method for infectious diseases. J. Infect. Chemother., 15: 62-69.
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Notomi, T; Okayama, H; Masubuchi, H; Yonekawa, T; Watanabe, K; Amino, N and Hase, T (2000). Loop-mediated isothermal amplification of DNA. Nucleic Acids. Res., 28: E63.
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Royer, S and Pare, D (2003). Conservation of total synaptic weight through balanced synaptic depression and potentiation. Nature. 422: 518-522.
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Salzman, NH; Ghosh, D; Huttner, KM; Paterson, Y and Bevins, CL (2003). Protection against enteric salmonellosis in transgenic mice expressing a human intestinal defensin. Nature. 422: 522-526.
21
Shehata, DM and Lu, X (2016). Development of a loop mediated isothermal amplification (LAMP) - surface enhanced raman spectroscopy (SERS) assay for the detection of Salmonella enterica serotype enteritidis. Theranostics. 6: 522.
22
Singh, G; Sithebe, A; Enitan, AM; Kumari, S; Bux, F and Stenstrom, TA (2017). Comparison of droplet digital PCR and quantitative PCR for the detection of Salmonella and its application for river sediments. J. Water Health. 15: 505-508.
23
Song, J; Mauk, MG; Hackett, BA; Cherry, S; Bau, HH and Liu, C (2016). Instrument-free point-of-care molecular detection of Zika virus. Anal. Chem., 88: 7289-7294.
24
Soria, MC; Soria, MA; Bueno, DJ and Terzolo, HR (2013). Comparison of 3 culture methods and PCR assays for Salmonella gallinarum and Salmonella pullorum detection in poultry feed. Poult. Sci., 92: 1505-1515.
25
Sun, J; Huang, L; Wei, Y; Wang, Y; Chen, D; Du, W; Wu, H; Feng, L and Liu, C (2015). Identification of three PPV1 VP2 protein-specific B cell linear epitopes using monoclonal antibodies against baculovirus-expressed recombinant VP2 protein. Appl. Microbiol. Biotechnol., 99: 9025-9036.
26
Xia, T; Jia, C; Ying, W and Wang, X (2016). Gene cloning, expression and polyclonal antibody preparation of Rab3A for protein interaction analysis. Springer plus., 5: 1705.
27
Zhang, L; Du, X; Chen, C; Chen, Z; Gong, N; Song, Y; Song, Y; Han, Q; Xia, X;Luo, H and Zhang, J (2019). Instrument-Free and Visual Detection of Salmonella Based on Magnetic Nanoparticles and an Antibody Probe Immunosensor. Int. J. Mol. Sci., 20(18).
28
Zhang, S; Kingsley, RA; Santos, RL; Andrews-Polymenis, H; Raffatellu, M; Figueiredo, J; Nunes, J; Tsolis, RM; Adams, LG and Baumler, AJ (2003). Molecular pathogenesis of Salmonella enterica serotype Typhimurium-induced diarrhea. Infect. Immun., 71: 1-12.
29
Zhang, L; Shi, Y; Chen, C; Han, Q; Chen, Q; Xia, X; Song, Y and Zhang, J (2019). Rapid, visual detection of Klebsiella pneumoniae using magnetic nanoparticles and an horseradish peroxidase-probe based immunosensor. J. Biomed. Nanotechnol., 15: 1061-1071.
30
Zhang, L; Shi, Y; Chen, C; Han, Q; Zhang, M; Yi, H; Song, Y; Xia, X and Zhang, J (2019). Visual and rapid detection of Klebsiella pneumoniae by magnetic immunocapture-loop-mediated isothermal amplification assay. Jundishapur J. Microbiol., 12: e90016.
31
Zhang, L; Wei, Q; Han, Q; Chen, Q; Tai, W; Zhang, J; Song, Y and Xia, X (2018). Detection of Shigella in milk and clinical samples by magnetic immunocaptured-loop-mediated isothermal amplification assay. Front. Microbiol., 9: 94.
32
Zhao, Y; Jiang, X; Qu, Y; Pan, R; Pang, X; Jiang, Y and Man, C (2017). Salmonella detection in powdered dairy products using a novel molecular tool. J. Dairy Sci., 100: 3480-3496.
33
Zheng, Q; Miks-Krajnik, M; Yang, Y; Lee, SM; Lee, SC and Yuk, HG (2016). Evaluation of real-time PCR coupled with immunomagnetic separation or centrifugation for the detection of healthy and sanitizer-injured Salmonella spp. on mung bean sprouts. Int. J. Food Microbiol., 222: 48-55.
34
Zhuang, L; Gong, J; Li, Q; Zhu, C; Yu, Y; Dou, X; Liu, X; Xu, B and Wang, C (2014). Detection of Salmonella spp. by a loop-mediated isothermal amplification (LAMP) method targeting bcfD gene. Lett. Appl. Microbiol., 59: 658-664.
35
ORIGINAL_ARTICLE
Serotypes, virulence genes and polymorphism of capsule gene cluster in Lactococcus garvieae isolated from diseased rainbow trout (Oncorhynchus mykiss) and mugger crocodile (Crocodylus palustris) in Iran
Background: Lactococcus garvieae causes lactococcosis in rainbow trout in many parts of the world. Aims: This study was conducted for the existence of the virulent factors and differentiation of the two serotypes in L. garvieae. Methods: Twenty-two strains of L. garvieae isolated from diseased rainbow trout from farms in different regions and mugger crocodile of Iran, were investigated. In order to rapidly detect the presence of the hly1, hly2, hly3, NADH oxidase, sod, pgm, adhPsaA, eno, LPxTG-3, adhCI, and adhCII virulence genes, two multiplex polymerase chain reaction (PCR) assays were developed. Also, simplex PCR method was used to identify the bacterial serotypes, CGC, LPxTG-2, Adhesion, and adhPav virulence genes using the specific primer. Results: All varieties of L. garvieae contained the hly1, hly2, hly3, NADH oxidase, pgm, adhPav, LPxTG-3, sod, eno, adhPsaA, adhCI, and CGC virulence genes. Also, adhCII gene was present in all strains except one of the isolates originated from mugger crocodile. In addition, LPxTG-2 gene was onlypresent in one of the isolates belonging to mugger crocodile. Adhesion gene was not present in all the strains. Interestingly, all the 22 strains originated from both hosts were identified as belonging to the serotype I. Based on the phylogenetic sequences of the capsule gene cluster, group all fish isolates into a cluster together with one isolate obtained from mugger crocodile. Conclusion: Further studies are recommended to investigate the role of virulence genes in L. garvieae and evaluate their pathogenicity to rainbow trout.
https://ijvr.shirazu.ac.ir/article_5437_af910d83f35a22378727c9b4d3ae354b.pdf
2020-03-01
26
32
10.22099/ijvr.2019.34317.5061
Iran
Lactococcus garvieae
Rainbow trout
Serotype
Virulence factors
R.
Salighehzadeh
1
Resident of Aquatic Animal Health, Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
AUTHOR
H.
Sharifiyazdi
sharifiy@shirazu.ac.ir
2
Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
LEAD_AUTHOR
M.
Akhlaghi
3
Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
AUTHOR
S.
Soltanian
4
Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
AUTHOR
Akhlaghi, M and Keshavarzi, M (2002). The occurrence of streptococcosis in the cultured rainbow trout of Fars province. Iran. J. Vet. Res., 2: 183-189.
1
Coutte, L; Alonso, S; Reveneau, N; Willery, E; Quatannens, B; Locht, C and Jacob-Dubuisson, F (2003). Role of adhesin release for mucosal colonization by a bacterial pathogen. J. Exp. Med., 197: 735-742. https://doi.org/10. 1084/jem.20021153.
2
Englen, MD and Kelley, LC (2000). A rapid DNA isolation procedure for the identification of Campylobacter jejuni by the polymerase chain reaction. Lett. Appl. Microbiol., 31: 421-426. https://doi.org/10.1046/j.1365-2672.2000.00841. x.
3
Erfanmanesh, A; Soltani, M; Pirali, E; Mohammadian, S and Taherimirghaed, A (2012). Genetic characterization of Streptococcus iniae in diseased farmed rainbow trout (Onchorhynchus mykiss) in Iran. Sci. World J., 2012: 1-6. http://dx.doi.org/10.1100/2012/594073.
4
Fukuda, Y; Tue, Y; Oinaka, D; Wada, Y; Yamashita, A; Urasaki, S; Yoshioka, S; Kimoto, K and Yoshida, T (2015). Pathogenicity and immunogenicity of non-agglutinating Lactococcus garvieae with Anti-KG (-) phenotype rabbit serum in Seriola spp. Fish. Pathol., 50: 200-206. https://doi.org/10.3147/jsfp.50.200.
5
Karsidani, SH; Soltani, M; Nikbakhat-Brojeni, G; Ghasemi, M and Skall, HF (2010). Molecular epidemiology of zoonotic streptococcosis/lactococcosis in rainbow trout (Oncorhynchus mykiss) aquaculture in Iran. Iran. J. Microbiol., 2: 198-209.
6
Mata, AI; Gibello, A; Casamayor, A; Blanco, MM; Domínguez, L and Fernández-Garayzábal, JF (2004). Multiplex PCR assay for detection of bacterial pathogens associated with warm-water streptococcosis in fish. Appl. Environ. Microbiol., 70: 3183-3187. https://doi.org/10.1128 /AEM.70.5.3183-3187.2004.
7
Meyburgh, CM; Bragg, RR and Boucher, CE (2017). Lactococcus garvieae: an emerging bacterial pathogen of fish. Dis. Aquat. Organ., 123: 67-79. https://doi.org/10. 3354/dao03083.
8
Miyauchi, E; Toh, H; Nakano, A; Tanabe, S and Morita, H (2012). Comparative genomic analysis of Lactococcus garvieae strains isolated from different sources reveals candidate virulence genes. Int. J. Microbiol., 2012: 1-6. http://dx.doi.org/10.1155/2012/728276.
9
Morita, H; Toh, H; Oshima, K; Yoshizaki, M; Kawanishi, M; Nakaya, K; Suzuki, T; Miyauchi, E; Ishii, Y; Tanabe, S and Murakami, M (2011). Complete genome sequence and comparative analysis of the fish pathogen Lactococcus garvieae. PLoS ONE. 6: e23184. https://doi. org/10.1371/journal.pone.0023184.
10
Ohbayashi, K; Oinaka, D; Hoai, TD; Yoshida, T and Nishiki, I (2017). PCR-mediated identification of the newly emerging pathogen Lactococcus garvieae serotype II from Seriola quinqueradiata and S. dumerili. Fish. Pathol., 52: 46-49. https://doi.org/10.3147/jsfp.52.46.
11
Ooyama, T; Hirokawa, Y; Minami, T; Yasuda, H; Nakai, T; Endo, M; Ruangpan, L and Yoshida, T (2002). Cell-surface properties of Lactococcus garvieae strains and their immunogenicity in the yellowtail Seriola quinqueradiata. Dis. Aquat. Org., 51: 169-177. https://doi.org/10.3354/ dao051169.
12
Raissy, M; Sarshoughi, M and Moumeni, M (2016). Molecular identification of some causative agents of warm-water streptococcosis by M-PCR in cultured rainbow trout, Chaharmahal-Bakhtiari Province, Iran. Iran. J. Fish. Sci., 15: 836-845. http://jifro.ir/article-1-2224-en.html.
13
Sharifiyazdi, H; Akhlaghi, M; Tabatabaei, M and Mostafavi Zadeh, SM (2010). Isolation and characterization of Lactococcus garvieae from diseased rainbow trout (Oncorhynchus mykiss, Walbaum) cultured in Iran. Iran. J. Vet. Res., 11: 342-350.
14
Shin, GW; Nho, SW; Park, SB; Jang, HB; Cha, IS; Ha, MA; Kim, YR; Dalvi, RS; Joh, SJ and Jung, TS (2009). Comparison of antigenic proteins from Lactococcus garvieae KG (−) and KG (+) strains that are recognized by olive flounder (Paralichthys olivaceus) antibodies. Vet. Microbiol., 139: 113-120. https://doi.org/10.1016/j.vetmic. 2009.05.007.
15
Sneath, PH and Sokal, RR (1973). Numerical taxonomy. The principles and practice of numerical classification. 1st Edn., San Francisco, USA, W. H. Freeman & Company. P: 573.
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Stipcevic, T; Piljac, T and Isseroff, RR (2005). Di-rhamnolipid from Pseudomonas aeruginosa displays differential effects on human keratinocyte and fibroblast cultures. J. Dermatol. Sci., 40: 141-143. https://doi.org/10. 1016/j.jdermsci.2005.08.005.
17
Tamura, K; Dudley, J; Nei, M and Kumar, S (2007). MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol. Biol. Evol., 24: 1596-1599. https://doi.org/10.1093/molbev/msm092.
18
Ture, M and Altinok, I (2016). Detection of putative virulence genes of Lactococcus garvieae. Dis. Aquat. Org., 119: 59-66. https://doi.org/10.3354/dao02981.
19
Türe, M; Haliloğlu, Hİ; Altuntaş, C; Boran, H and Kutlu, İ (2014). Comparison of experimental susceptibility of Rainbow Trout (Oncorhynchus mykiss), Turbot (Psetta maxima), Black Sea Trout (Salmo trutta labrax) and Sea Bass (Dicentrarchus labrax) to Lactococcus garvieae. Turk. J. Fish. Aquat. Sci., 14: 507-513. https://doi.org/10. 4194/1303-2712-v14_2_22.
20
Vanaporn, M; Wand, M; Michell, SL; Sarkar-Tyson, M; Ireland, P; Goldman, S; Kewcharoenwong, C; Rinchai, D; Lertmemongkolchai, G and Titball, RW (2011). Superoxide dismutase C is required for intracellular survival and virulence of Burkholderia pseudomallei. Microbiology. 157: 2392-2400. https://doi.org/10.1099/ mic.0.050823-0.
21
Yoshida, T; Endo, M; Sakai, M and Inglis, V (1997). A cell capsule with possible involvement in resistance to opsonophagocytosis in Enterococcus seriolicida isolated from yellowtail Seriola quinqueradiata. Dis. Aquat. Org., 29: 233-235. https://doi.org/10.3354/dao029233.
22
ORIGINAL_ARTICLE
Emergence of variant avian infectious bronchitis virus in India
Background: Infectious bronchitis virus (IBV) is the etiological agent of an acute and highly contagious disease. Infectious bronchitis (IB) affects chicken of all ages and poses major economic loses to the poultry industry worldwide. The continuous evolution of the spike protein (S1) of IBV is responsible for the prevalence of many serotypes/genotypes around the world. Multiple lineages of IBV strains have been detected in chicken flocks in India since 2003. Aims: To detect IBV genotypes prevalent in India. Methods: Organ samples from 20 IBV-positive flocks with variable clinical signs were used for the amplification of the S1 gene of IBV by reverse transcriptase-polymerase chain reaction (RT-PCR). Results: Positive PCR amplicons were sequenced. Sequence analysis showed that 14 field isolates belonged to the GI-1 genetic lineage (Mass 41 serotype), two field isolates belonged to the GI-13 (UK 4/91 variant IBV strain), one field isolate grouped with GIII, GV, and GVI genetic lineage and three belonged to a variant genotype unique to India (GI-24). Phylogenetic analysis also showed a similar type of grouping within the field isolates. Among the fourteen GI-1 isolates, 12 were isolated between 2003 and 2006 and only two were isolated between 2009 and 2011. The two field isolates belonging to GI-13 were isolated in 2007, another one belonging to GIII, GV, and GVI was isolated in 2010 and three field isolates were not close to any reference IBV sequences isolated in 2006 (IND-TN-168-06), 2010 (IND-TN-280-10) and 2011 (IND-TN-290-11). Conclusion: A unique variant of IBV is emerging in India (GI-24). Our findings will have important implications for future vaccine intervention.
https://ijvr.shirazu.ac.ir/article_5438_13e6d89f435122d79f0851db464d5943.pdf
2020-03-01
33
39
10.22099/ijvr.2019.32861.4900
Infectious bronchitis virus
Phylogeny
Sequence
Spike protein 1 gene
Variant infectious bronchitis virus
A.
Raja
raja.a@tanuvas.ac.in
1
Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu, India
LEAD_AUTHOR
G.
Dhinakar Raj
2
Center for Animal Health Studies, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu, India
AUTHOR
K.
Kumanan
3
Bioinformatics Centre and ARIS Cell, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu, India
AUTHOR
Bande, F; Arshad, SS; Omar, AR; Hair-Bejo, M; Mahmuda, A and Nair, V (2017). Global distributions and strain diversity of avian infectious bronchitis virus: a review. Anim. Health Res. Rev., 18: 70-83.
1
Bayry, J; Goudar, MS; Nighot, PK; Kshirsagar, SG; Ladman, BS and Gelb, J (2005). Emergence of a nephropathogenic avian infectious bronchitis virus with a novel genotype in India. J. Clin. Microbiol., 43: 916-918.
2
Boursnell, ME; Brown, TD; Foulds, IJ; Green, PF; Tonsley, FM and Binns, MM (1987). Completion of the sequence of the genome of the corona virus avian infectious bronchitis virus. J. Gen. Virol., 68: 57-77.
3
Casais, R; Dove, B; Cavanagh, D and Britton, P (2003). Recombinant avian infectious bronchitis virus expressing a heterologous spike gene demonstrates that the spike protein is a determinant of cell tropism. J. Virol., 77: 9084-9089.
4
Cavanagh, D (1983). Coronavirus IBV glycopolypeptides: size of their polypeptide moieties and nature of their oligosaccharide. J. Gen. Virol., 64: 1187-1191.
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Cavanagh, D (1997). Nidoviridaes: a new order comprising Coronaviridae and Arteriviridae. Arch. Virol., 142: 629-633.
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Cavanagh, D (2001). Commentary. A nomenclature for avian Coronavirus isolates and the question of species status. Avian Pathol., 30: 581-598.
7
Cavanagh, D (2007). Coronavirus avian infectious bronchitis virus. Vet. Res., 38: 281-297.
8
Cavanagh, D; Davis, PJ; Cook, JK; Li, D; Kant, A and Koch, G (1992). Location of the amino acid difference in the S1 spike glycoprotein subunit of closely related serotypes of infectious bronchitis virus. Avian Pathol., 21: 33-43.
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Cavanagh, D; Elus, MM and Cook, JK (1997). Relationship between variation in the S1 spike protein of infectious bronchitis virus and the extent of cross-protection in vivo. Avian Pathol., 26: 63-74.
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Cavanagh, D; Picault, JP; Gough, RE; Hess, M; Mawditt, K and Britton, P (2005). Variation in the spike protein of the 793/B type of infectious bronchitis virus, in the field and during alternate passage in chickens and embryonated eggs. Avian Pathol., 34: 20-25.
11
Chen, HW; Huang, YP and Wang, CH (2009). Identification of Taiwan and China-like recombinant avian infectious bronchitis viruses in Taiwan. Virus Res., 140: 121-129.
12
Ducatez, MF; Martin, AM; Owoade, AA; Olatoye, IO; Alkali, BR; Maikano, I; Snoeck, CJ; Sausy, A; Cordioli, P and Muller, CP (2009). Characterization of a new genotype and serotype of infectious bronchitis virus in Western Africa. J. Gen. Virol., 90: 2679-2685.
13
Elankumaran, S; Balachandran, C; Chandran, NDJ; Roy, P; Albert, A and Manickam, R (1999). Serological evidence for a 793/B related avian infectious bronchitis virus in India. Vet. Rec., 144: 299-300.
14
Farsang, A; Ros, C; Renstrom, LH; Baule, C; Soos, T and Belak, S (2002). Molecular epizootiology of infectious bronchitis virus in Sweden indicating the involvement of a vaccine strain. Avian Pathol., 31: 229-236.
15
Gaba, A; Dave, H; Paul, JK and Prajapati, KS (2010). Isolation, identification and molecular characterization of IBV variant from outbreak of visceral gout commercial broilers. Vet. World. 3: 375-377.
16
Jakhesara, SJ; Nath, B; Pal, JK; Joshi, CG and Kumar, S (2018). Emergence of a genotype I variant of avian infectious bronchitis virus from Northern part of India. Acta Trop., 183: 57-60.
17
Kant, A; Koch, G; van Roozelaar, DJ; Kusters, JG; Poelwik, FA and van der Zeijst, BA (1992). Location of antigenic sites defined by neutralizing monoclonal antibodies on the S1 avian infectious bronchitis virus glycopolypeptide. J. Gen. Virol., 73: 591-596.
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Keeler, CLJr; Reed, KL; Nix, WA and Gelb, JJr (1998). Serotype identification of avian infectious bronchitis virus by RT-PCR of the peplomer (S-1) gene. Avian Dis., 42: 275-284.
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Koch, L; Hartog, L; Kant, A and van Roozelaar, DJ (1990). Antigenic domains on the peplomer protein of avian infectious bronchitis virus: correlation with biological functions. J. Gen. Virol., 71: 1929-1935.
20
Kumanan, K; Tamizh Selvam, N; Dhinakar Raj, G and Nachimuthu, K (2004). Molecular epizootiology of infectious bronchitis isolates in Tamil Nadu indicating the possible involvement of a vaccine strain. Ind. Vet. J., 81: 1307-1310.
21
Kuo, L; Godeke, GJ; Raamsman, MJ; Masters, PS and Rottier, PJ (2000). Retargeting of coronavirus by substitution of the spike glycoprotein ectodomain: crossing the host cell species barrier. J. Virol., 74: 1393-1406.
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Kusters, JG; Niesters, HG; Lenstra, JA; Horzinek, MC and van der Zeijst, BA (1989). Phylogeny of antigenic variants of avian coronavirus IBV. Virology. 169: 217-221.
23
Lee, CW; Hilt, DA and Jackwood, MW (2003). Typing of field isolates of infectious bronchitis virus based on the sequence of the hypervariable region in the S1 gene. J. Vet. Diagn. Invest., 15: 344-348.
24
Lim, TH; Lee, HJ; Lee, DJ; Lee, JN; Park, JK; Youn, HN; Kim, MS; Lee, JB; Park, SY; Choi, IS and Song, CS (2011). An emerging recombinant cluster of nephro-pathogenic strains of avian infectious bronchitis virus in Korea. Infect. Genet. Evol., 11: 678-685.
25
Lin, SY and Chen, HW (2017). Infectious bronchitis virus variants: molecular analysis and pathogenicity investigation. Int. J. Mol. Sci., 18: 2030. doi: 10.3390/ ijms18102030.
26
Mahmooda, ZH; Sleman, RR and Uthman, AU (2011). Isolation and molecular characterization of Sul/01/09 avian infectious bronchitis virus, indicates the emergence of a new genotype in the Middle East. Vet. Microbiol., 150: 21-27.
27
Moore, KM; Jackwood, MW and Hilt, DA (1997). Identification of amino acids involved in a serotype and neutralization specific epitopes within the S1 subunit of avian infectious bronchitis virus. Arch. Virol., 142: 2249-2256.
28
Parveen, R; Farooq, L; Ahangar, S; Nazki, S; Dar, Z; Dar, T; Kamil, S and Dar, P (2017). Genotyping and phylogenetic analysis of infectious bronchitis virus isolated from broiler chickens in Kashmir. Virus Dis., 28: 434-438.
29
Patel, BH; Bhimani, MP; Bhanderi, BB and Jhala, MK (2015). Isolation and molecular characterization of nephropathic infectious bronchitis virus isolates of Gujarat state, India. Virus Dis., 26: 42-47.
30
Sadri, N; Ghalyanchilangeroudi, A; Fallah Mehrabadi, MH; Shayeganmehr, A; Sediqian, MS; Jabbarifakhr, M; Hosseini, H; Hamdan, AM and Mousavi, FS (2018). Genotyping of avian infectious bronchitis virus in Afghanistan (2016-2017): the first report. Iran. J. Vet. Res., 20: 60-63.
31
Sályi, G (1999). Certain disturbances in nitrogen metabolism of poultry; gout, urolithiasis and baby chick nephropathy. A review of the literature . Magyar Állatorvosok Lapja. 121: 91-99 (in Hungarian with English summary).
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Sukumar, S and Prabhakar, TG (1993). An outbreak of infectious bronchitis among poultry in Tamil Nadu. Ind. J. Anim. Sci., 63: 820-822.
33
Sumi, V; Singh, SD; Dhama, K; Gowthaman, V; Barathidasan, R and Sukumar, K (2012). Isolation and molecular characterization of infectious bronchitis virus from recent outbreaks in broiler flocks reveals emergence of novel strain in India. Trop. Anim. Health Pro., 44: 1791-1795.
34
Suresh Kumar, K; Dhinakar Raj, G; Raja, A and Ramadass, P (2007). Genotyping of avian infectious bronchitis viruses from India. Ind. J. Biotech., 6: 45-51.
35
Valastro, V; Holmes, EC; Britton, P; Fusaro, A; Jackwood, MW; Cattoli, G and Monne, I (2016). S1-gene based phylogeny of infectious bronchitis virus: an attempt of harmonize virus classification. Infect. Genet. Evol., 39: 349-364.
36
Wang, L; Junker, D; Hock, L; Ebiary, E and Collisson, EW (1994). Evolutionary implications of genetic variations in the S1 gene of infectious bronchitis virus. Virus Res., 34: 327-338.
37
Wang, CH and Tsai, CT (1996). Genetic grouping for the isolates of avian infectious bronchitis viruses in Taiwan. Arch. Virol., 141: 1677-1688.
38
ORIGINAL_ARTICLE
The association of genetic polymorphisms of bone formation genes with canine hip dysplasia
Background: Canine hip dysplasia (CHD) is an orthopedic disorder characterized by abnormal laxity of the hip joint. It is considered multifactorial and polygenic and affects predominantly medium and large sized dog breeds. Aims: The aim of this study was to identify CHD associated polymorphisms in chromosomal regions on CFA19, CFA24, CFA26, and CFA34. Methods: Blood samples from 60 dogs of different breeds were collected and genotyped, including 46 cases and 14 controls. After sequencing and single nucleotide polymorphism (SNP) determination of the target regions, an individual SNP analysis with a c2 statistic was performed based on the comparison of allele frequencies in cases and controls. Results: A significant association was observed between CHD and a T/C SNP on CFA19, which harbors genes involved in bone metabolism. No other significant association was found in the study and previously identified SNPs cannot be validated as related to CHD. Conclusion: Further research is warranted to identify CHD-associated polymorphisms in order to develop a genotype-based diagnosis and selection approach.
https://ijvr.shirazu.ac.ir/article_5403_b1fefd88fce006dc3a153e0220cf27d5.pdf
2020-03-01
40
45
10.22099/ijvr.2019.31251.4713
Association
dog
Hip dysplasia
Selection
SNP
I.
Akis
1
Department of Basic Sciences, Faculty of Veterinary Medicine, Istanbul University-Cerrahpasa, 34320, Avcilar, Istanbul, Turkey
AUTHOR
A.
Ates
atiates@istanbul.edu.tr
2
Department of Basic Sciences, Faculty of Veterinary Medicine, Istanbul University-Cerrahpasa, 34320, Avcilar, Istanbul, Turkey
LEAD_AUTHOR
G.
Atmaca
3
Ph.D. Student in Biochemistry, Department of Basic Sciences, Faculty of Veterinary Medicine, Istanbul University-Cerrahpasa, 34320, Avcilar, Istanbul, Turkey
AUTHOR
K. O.
Oztabak
4
Department of Basic Sciences, Faculty of Veterinary Medicine, Istanbul University-Cerrahpasa, 34320, Avcilar, Istanbul, Turkey
AUTHOR
F.
Esen Gursel
5
Department of Basic Sciences, Faculty of Veterinary Medicine, Istanbul University-Cerrahpasa, 34320, Avcilar, Istanbul, Turkey
AUTHOR
H.
Yardibi
6
Department of Basic Sciences, Faculty of Veterinary Medicine, Istanbul University-Cerrahpasa, 34320, Avcilar, Istanbul, Turkey
AUTHOR
K.
Altunatmaz
7
Department of Clinical Sciences, Faculty of Veterinary Medicine, Istanbul University-Cerrahpasa, 34320, Avcilar, Istanbul, Turkey
AUTHOR
E.
Eravci Yalin
8
Department of Clinical Sciences, Faculty of Veterinary Medicine, Istanbul University-Cerrahpasa, 34320, Avcilar, Istanbul, Turkey
AUTHOR
M.
Karabagli
9
Department of Clinical Sciences, Faculty of Veterinary Medicine, Istanbul University-Cerrahpasa, 34320, Avcilar, Istanbul, Turkey
AUTHOR
Baguma-Nibasheka, M; MacFarlane, LA and Murphy, PR (2012). Regulation of fibroblast growth factor-2 expression and cell cycle progression by an endogenous antisense RNA. Genes. 3: 505-520.
1
Bartolome, N; Segarra, S; Artieda, M; Francino, O; Sanchez, E; Szczypiorska, M; Casellas, J; Tejedor, D; Cerdeira, J; Martinez, A; Velasco, A and Sanchez, A (2015). A genetic predictive model for canine hip dysplasia: integration of genome wide association study (GWAS) and candidate gene approaches. PLoS ONE. 10: e0122558. https://doi.org/10.1371/journal.pone.0122558.
2
Boyce, BF; Xing, L; Yao, Z; Yamashita, T; Shakespeare, WC; Wang, Y; Metcalf, CA; Sundaramoorthi, R; Dalgarno, DC; Iuliucci, JD and Sawyer, TK(2006). SRC inhibitors in metastatic bone disease. Clin. Cancer Res., 12: 6291-6295.
3
Chase, K; Lawler, DF; Adler, FR; Ostrander, EA and Lark, KG (2004). Bilaterally asymmetric effects of quantitative trait loci (QTLs): QTLs tahat affect laxity in the right versus left coxofemoral (hip) joints of the dog (Canis familiaris). Am. J. Med. Genet. A. 124: 239-247.
4
Chase, K; Lawler, DF; Carrier, DR and Lark, KG (2005). Genetic regulation of osteoarthritis: a QTL regulating cranial and caudal acetabular osteophyte formation in the hip joint of the dog (Canis familiaris). Am. J. Med. Genet. A. 135: 334-335.
5
Fels, L and Distl, O (2014). Identification and validation of quantitative trait loci (QTL) for canine hip dysplasia (CHD) in German Shepherd dogs. PLOS ONE. 9: e96618. https://doi.or/10.1371/journal.pone.0096618.
6
Friedenberg, SG; Zhu, L; Zhang, Z; Foels, WV; Schweitzer, PA; Wang, W; Fisher, PJ; Dykes, NL; Corey, E; Vernier-Singer, M; Jung, SW; Sheng, X; Hunter, LS; McDonough, SP; Lust, G; Bliss, SP; Krotscheck, U; Gunn, TM and Todhunter, RJ (2011). Evaluation of a fibrillin 2 gene haplotype associated with hip dysplasia and incipient osteoarthritis in dogs. Am. J. Vet. Res., 72: 530-540.
7
Fries, CL and Remedios, AM (1995). The pathogenesis and diagnosis of canine hip dysplasia: a review. Can. Vet. J., 36: 494-502.
8
Guo, G; Zhou, Z; Wang, Y; Zhao, K; Zhu, L; Lust, G; Hunter, L; Friedenberg, S; Li, J; Zhang, Y; Harris, S; Jones, P; Sandler, J; Krotscheck, U; Todhunter, R and Zhang, Z (2011). Canine hip dysplasia is predictable by genotyping. Osteoarthr. Cartilage. 19: 420-429.
9
Hou, Y; Wang, Y; Lu, X; Zhang, X; Zhao, Q; Todhunter, RJ and Zhang, Z (2013). Monitoring hip and elbow dysplasia achieved modest genetic improvement of 74 dog breeds over 40 years in USA. PLOS ONE. 8:e76390. https://doi.org/10.1371/journal.pone.0076390.
10
Janutta, V; Hamann, H and Distl, O (2006). Complex segregation analysis of canine hip dysplasia in German shepherd dogs. J. Hered., 97: 13-20.
11
Kaneene, JB; Mostosky, UV and Miller, R (2009). Update of a retrospective cohort study of changes in hip joint phenotype of dogs evaluated by the OFA in The United States, 1989-2003. Vet. Surg., 38: 398-405.
12
Lavrijsen, ICM; Leegwater, PAJ; Martin, AJ; Harris, SJ; Tryfonidou, MA; Heuven, HCM and Hazewinkel, HAW (2014). Genome wide analysis indicates genes for basement membrane and cartilage matrix proteins as candidates for hip dysplasia in Labrador Retrievers. PLOS ONE. 9: e87735. https://doi.org/10.1371/journal.pone.0087735.
13
Lewis, TW; Blott, SC and Woolliams, JA (2010). Genetic evaluation of hip score in UK Labrador Retrievers. PLOS ONE. 5: e12797. https://doi.org/10.1371/journal.pone. 0012797.
14
Lewis, TW; Woolliams, JA and Blott, SC (2010). Genetic evaluation of the nine component features of hip score in UK Labrador Retrievers. PLOS ONE. 5: e13610. https:// doi.org/10.1371/journal.pone.0013610.
15
Liu, L; Channavajhala, PL; Rao, VR; Moutsatsos, I; Wu, L; Zhang, Y; Lin, LL and Qiu, Y (2009). Proteomic characterization of the dynamic KSR-2 interactome, a signaling scaffold complex in MAPK pathway. Biochim. Biophys. Acta. 1794: 1485-1495.
16
Maki, K; Janss, LLG; Groen, AF; Liinamo, AE and Ojala, M (2004). An indication of major genes affecting hip and elbow dysplasia in four Finnish dog populations. Hered., 92: 402-408.
17
Marschall, Y and Distl, O (2007). Mapping quantitative trait loci for canine hip dysplasia in German Shepherd dogs. Mamm. Genome. 18: 861-870.
18
Miyazaki, T; Tanaka, S; Sanjay, A and Baron, R (2006). The role of c-Src kinase in the regulation of osteoclast function. Mod. Rheumatol., 16: 68-74.
19
Nakano, R; Edamura, K; Nakayama, T; Narita, T; Okabayashi, K and Sugiya, H (2015). Fibroblast growth factor receptor-2 vontributes to the basic fibroblast growth factor-induced neuronal differentiation in canine bone marrow stromal cells via phosphoinositide 3-Kinase/Akt signaling pathway. PLOS ONE. 10: e0141581. doi: 10.1371/journal.pone.0141581.
20
Pfahler, S and Distl, O (2012). Identification of quantitative trait loci (QTL) for canine hip dysplasia and canine elbow dysplasia in Bernese mountain dogs. PLOS ONE. 7: e49782. https://doi.org/10.1371/journal.pone.0049782.
21
Sánchez-Molano, E; Pong-Wong, R; Clements, DN; Blott, SC; Wiener, P and Wooliams, JA (2015). Genomic prediction of traits related to canine hip dysplasia. Front. Genet., 6: 97. doi: 10.3389/fgene.2015.00097.
22
Sánchez-Molano, E; Woolliams, JA; Blott, SC and Wiener, P (2013). Assessing the impact of genomic selection against hip dysplasia in the Labrador Retriever dog. J. Anim. Breed. Genet., 131: 134-145.
23
Sánchez-Molano, E; Woolliams, JA; Pong-Wong, R; Clements, DN; Blott, SC and Wiener, P (2014). Quantitative trait loci mapping for canine hip dysplasia and its related traits in UK Labrador Retrievers. BMC Genomics. 15: 833.
24
Stock, KF and Distl, O (2010). Simulation study on the effects of excluding offspring information for genetic evaluation versus using genomic markers for selection in dog breeding. J. Anim. Breed. Genet., 127: 42-52.
25
Tamura, K; Stecher, G; Peterson, D; Filipski, A and Kumar, S (2013). MEGA6: molecular evolutionary genetics analysis version 6.0. Mol. Biol. Evol., 30: 2725-2729.
26
Todhunter, RJ; Mateescu, R; Lust, G; Burton-Wurster, NI; Dykes, NL; Bliss, SP; Williams, AJ; Vernier-Singer, M; Corey, E; Harjes, C; Quaas, RL; Zhang, Z; Gilbert, RO; Volkman, D; Casella, G; Wu, R and Acland, GM (2005). Quantitative trait loci for hip dysplasia in a crossbred canine pedigree. Mamm. Genome. 16: 720-730.
27
Wang, G and Beier, F (2005). Rac1/Cdc42 and RhoA GTPases antagonistically regulate chondrocyte pro-liferation, hypertrophy, and apoptosis. J. Bone Min. Res., 20: 1022-1031.
28
Willis, MB (1997). A review of the progress in canine hip-dysplasia control in Britain. J. Am. Vet. Med. Assoc., 210: 1480-1482.
29
Zhou, Z; Sheng, X; Zhang, Z; Zhao, K; Zhu, L; Guo, G; Friedenberg, SG; Hunter, LS; Vandenberg-Foels, WS; Hornbuckle, WE; Krotscheck, U; Corey, E; Moise, NS; Dykes, NL; Li, J; Xu, S; Du, L; Wang, Y; Sandler, J; Acland, GM; Lust, G and Todhunter, RJ (2010). Differential genetic regulation of canine hip dysplasia and osteoarthritis. PLOS ONE. 5: e13219. https://doi.org/10. 1371/journal.pone.0013219.
30
ORIGINAL_ARTICLE
Effect of soy isoflavones on implantation losses in Wistar rat: implication of progesterone receptors, vascular endothelial growth factor and estradiol receptors alpha
Background: Implantation is a crucial period determining the success of a full pregnancy. Endocrine disruptors such as phytoestrogens (PEs) were thought to adversely influence embryonic implantations. However, the mechanism by which they upset implantation was not fully elucidated. Aims: The effect of administering soy isoflavones on the implantation of Wistar rats was studied through the detection of progesterone receptors (PR), vascular endothelial growth factor (VEGF), and estradiol receptor alpha (ER-α) protein expression at gestation day 6 (GD6). Methods: Eighteen cyclic female Wistar rats were distributed into two groups, group A: control (n=9) were fed with a casein based diet, and group B (n=9) were fed with a casein diet and gavaged 50 mg/kg/day soy isoflavones’ extract 40% starting from gestation day zero (GD0) to GD6. Feed intake, body weight (BW), body gain, and uterine weights were recorded. At the end of GD6 the number of corpora lutae (CLs) and implantation rates were recorded. Histopathology and immunohistochemistry (IHC) for PR, VEGF, and ER-α protein expression in implanted uteri were performed. Results: Soy isoflavones significantly reduced feed intake, weight gain, uterine weights CL numbers, and implantation rates of the treated pregnant dams. The endometrium of the soy treated dams showed less proliferation than that of the control. Immunostaining percentage of PR and VEGF proteins significantly reduced in soy treated dams compared to the control. However, the mean expression percentage of ER-α exhibited significant elevation in the soy treated dams in comparison to the control group. Conclusion: Implantation losses caused by soy isoflavones seemed to be due to the down regulation of PR that failed to down regulate ER-α action and decreased VEGF production.
https://ijvr.shirazu.ac.ir/article_5401_81b73ce9d5b9ced21583b8643d24a3d0.pdf
2020-03-01
46
51
10.22099/ijvr.2019.31247.4717
ER-α
implantation
Isoflavones
progesterone
VEGF
D. H.
Elsayed
1
Department of Theriogenology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt
AUTHOR
H. M. A.
Abdelrazek
hebaabdelrazekvet@gmail.com
2
Department of Physiology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt
LEAD_AUTHOR
D. A.
Eltamany
3
Nutrition and Food Science, Home Economic Department, Faculty of Education, Suez Canal University, Ismailia, Egypt
AUTHOR
H. M.
Ebaid
4
Department of Zoology, Faculty of Sciences, Suez Canal University, Ismailia, Egypt
AUTHOR
A. M.
El-Nahla
5
Department of Physiology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt
AUTHOR
Achache, H and Revel, A (2006). Endometrial receptivity markers, the journey to successful embryo implantation. Hum. Reprod. Update. 12: 731-746.
1
Amal, M; Abdoon, A; Ismail, A; Heba, M; Hend, M and Gihan, G (2014). Teratogenic effects of dietary genistein and daidzein are mediated by over regulation of Oct-4 and down regulation of Cdx2 expression in post implantation Albino rat embryos. Int. J. Chem. Environ. Biol. Sci., 2: 129-135.
2
Amini, L; Tehranian, N; Movahedin, M; Tehrani, FR and Ziaee, S (2015). Antioxidants and management of polycystic ovary syndrome in Iran: a systematic review of clinical trials. Iran. J. Reprod. Med., 13: 1-8.
3
Bancroft, JD and Gamble, M (2008). Theory and practice of histological techniques. 6th Edn., Churchill Livingstone, UK, Elsevier Health Sciences. PP: 53-134.
4
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Ebaid, HM; Elgawish, RAR; Abdelrazek, HM; Gaffer, G and Tag, HM (2016). Prenatal exposure to soy isoflavones altered the immunological parameters in female rats. Int. J. Toxicol., 35: 274-283.
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Elgawish, RAR; Rahman, HGA and Abdelrazek, HMA (2015). Green tea extract attenuates CCl4-induced hepatic injury in male hamsters via inhibition of lipid peroxidation and p53-mediated apoptosis. Toxicol. Rep., 2: 1149-1156.
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Helmy, S; Emarah, H and Abdelrazek, H (2014). Estrogenic effect of soy phytoestrogens on the uterus of ovariectomized female rats. Clin. Pharmacol. Biopharm., S2:001 doi:10.4172/2167-065X.S2-001.
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38
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41
ORIGINAL_ARTICLE
Comparison of multiplex and ordinary PCR for diagnosis of paratuberculosis and tuberculosis in blood samples (buffy coat) of cattle and buffaloes
Background: Paratuberculosis and tuberculosis (TB) caused by Mycobacterium avium paratuberculosis (MAP) and Mycobacterium tuberculosis complex (MTC), respectively are economically important, chronic debilitating diseases affecting the dairy herds and are also potential zoonotic threats. Aims: Differential diagnosis of paratuberculosis and TB in blood samples of cattle and buffaloes. Methods: In this study, an in-house developed multiplex polymerase chain reaction (PCR) targeting MAP, Mycobacterium bovis and Mycobacterium smegmatis was used in blood samples (buffy coat) parallel with IS900 PCR and esxB PCR for diagnosis of paratuberculosis and TB, respectively; in a total of 202 cattle and buffaloes. Results: Out of 202 animals, 12 (5.9%) and 17 (8.4%) animals were positive for MAP by multiplex PCR and IS900 PCR, respectively; from which only 8 (4%) animals were positive by both tests; whereas 4 and 9 animals were exclusively positive by multiplex PCR and IS900 PCR, respectively. None of the animals were found to be positive for M. bovis and M. smegmatis by the multiplex PCR. However, the esxB PCR detected 13 (6.4%) animals positive for TB. In fact, 3 (1.5%) animals were found to be co-infected by both paratuberculosis and TB. Conclusion: The in-house multiplex PCR detected MAP in buffy coat and there was a fair degree of agreement between the multiplex PCR and IS900 PCR in detection of MAP DNA though the latter detected more number of animals to be positive for MAP. Besides, esxB PCR showed a high diagnostic potential and can be used for diagnosis of TB from blood.
https://ijvr.shirazu.ac.ir/article_5400_a2c2bb1d5dc5f23adb44aedd96cddf6a.pdf
2020-03-01
52
56
10.22099/ijvr.2019.30677.4626
Blood samples
Multiplex PCR
paratuberculosis
Tuberculosis
D.
Brahma
1
MVSc Student in Veterinary Microbiology, Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, Punjab, India
AUTHOR
D.
Narang
deeptivet@rediffmail.com
2
Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, Punjab, India
LEAD_AUTHOR
M.
Chandra
3
Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, Punjab, India
AUTHOR
S. T.
Singh
4
Directorate of Livestock Farm, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, Punjab, India
AUTHOR
Bakshi, CS; Shah, DH; Verma, R; Singh, RK and Malik, M (2005). Rapid differentiation of Mycobacterium bovis and Mycobacterium tuberculosis based on a 12.7-kb fragment by a single tube multiplex-PCR. Vet. Microbiol., 109: 211-216.
1
Barrington, GM; Gay, JM; Eriks, IS; Davis, WC; Evermann, JF; Emerson, C; O’Rourke, JL; Hamilton, MJ and Bradway, DS (2003). Temporal patterns of diagnostic results in serial samples from cattle with advanced paratuberculosis infections. J. Vet. Diagn. Invest., 15: 195-200.
2
Bhide, M; Chakurkar, E; Tkacikova, L; Barbudhe, S; Novak, M and Mikula, I (2006). IS900-PCR-based detection and characterization of Mycobacterium avium subsp. paratuberculosis from buffy coat of cattle and sheep. Vet. Microbiol., 112: 33-41.
3
Bolaños, CAD; de Paula, CL; Guerra, ST; Franco, MMJ and Ribeiro, MG (2017). Diagnosis of mycobacteria in bovine milk: an overview. Rev. Inst. Med. Trop. São Paulo. 59: e40.
4
Brahma, D; Narang, D; Chandra, M; Gupta, K; Singh, A and Kaur, G (2017). Diagnosis of Mycobacterial infections (Tuberculosis and Paratuberculosis) in tissue samples using molecular (inhouse multiplex PCR, PCR and TaqMan real-time PCR), histopathology and immuno-histochemical techniques. Trop. Biomed., 34: 1-17.
5
Carvalho, IA; Vinicius, CEB; Souza, IM; Zanardo, LG; Filho, JDR; Gomes, MJP and Moreira, MAS (2012). Diagnosis of Paratuberculosis in cattle: microbiological culture, serology and PCR. Braz. J. Microbiol., 43: 581-585.
6
Dikshit, M; Sharma, RJ; Adsool, AD and Chaphalkar, SR (2012). ESAT-6 and CFP-10 proteins of Mycobacterium tuberculosis in making diagnostic tool for TB. J. Biotechnol. Lett., 3: 28-30.
7
Franco, MM; Paes, AC; Ribeiro, MG; Pantoja, JC; Santos, AC; Miyata, M; Leite, CQ; Motta, RG and Listoni, FJ (2013). Occurrence of mycobacteria in bovine milk samples from both individual and collective bulk tanks at farms and informal markets in the southeast region of Sao Paulo, Brazil. BMC Vet. Res., 9: 85.
8
Gao, A; Odumeru, J; Raymond, M; Hendrick, S; Duffield, T and Mutharia, L (2009). Comparison of milk culture, direct and nested polymerase chain reaction (PCR) with fecal culture based on samples from dairy herds infected with Mycobacterium avium subsp. paratuberculosis. Can. J. Vet. Res., 73: 58-64.
9
Gümüşsoy, KS; İça, T; Abay, S; Aydin, F and Hizlisoy, H (2015). Serological and molecular diagnosis of paratuberculosis in dairy cattle. Turk. J. Vet. Anim. Sci., 39: 147-153.
10
Gwozdz, JM; Thompson, KG; Manktelow, BW; Murray, A and West, DM (2000). Vaccination against para-tuberculosis of lambs already infected experimentally with Mycobacterium avium subspecies paratuberculosis. Aust. Vet. J., 78: 560-566.
11
Hermon-Taylor, J (2009). Mycobacterium avium subspecies paratuberculosis, Crohn’s disease and the Doomsday scenario. Gut Pathog., 1: 15. doi: 10.1186/1757-4749-1-15.
12
Juste, RA; Garrido, JM; Geijo, MV; Elguezabal, N; Aduriz, G; Atxaerandio, R and Sevilla, I (2005). Comparison of blood PCR and ELISA for detection of Mycobacterium avium subsp. paratuberculosis infection in sheep and cattle. J. Vet. Diagn. Invest., 17: 354-359.
13
Khol, JL; Wassertheurer, M; Sodoma, E; Revilla-Fernández, S; Damoser, J; Österreicher, E; Dünser, M; Kleb, U and Baumgartner, W (2013). Long-term detection of Mycobacterium avium subspecies para-tuberculosis in individual and bulk tank milk from a dairy herd with a low prevalence of Johne’s disease. J. Dairy Sci., 96: 3517-3524.
14
Naser, SA; Ghobrial, G; Romero, C and Valentine, JF (2004). Culture of Mycobacterium avium subspecies paratuberculosis from the blood of patients with Crohn’s disease. Lancet. 364: 1039-1044.
15
OIE (2009). Chap. 2.4.6: Bovine tuberculosis. Terrestrial Manual. World Organization for Animal Health, Paris, France.
16
OIE (2019a). http://www.oie.int/en/animal-health-in-the-world/animal-diseases/Paratuberculosis/ Accessed on 04. 02.2019.
17
OIE (2019b). http://www.oie.int/en/animal-health-in-the-world/animal-diseases/Bovine-tuberculosis/ Accessed on 04.02.2019. Olsen, I; Barletta, RG and Thoen, CO (2010). Mycobacterium. In: Gyles, CL; Prescott, JF; Songer, JG and Thoen, CO (Eds.), Pathogenesis of bacterial infections in animals. (4th Edn.), Ames, Iowa, Wiley-Blackwell. PP: 113-132.
18
Pinxteren, LAHV; Ravn, P; Agger, EM; Pollock, J and Andersen, P (2000). Diagnosis of tuberculosis based on the two specific antigens ESAT-6 and CFP-10. Clin. Diagn. Lab. Immunol., 7: 155-160.
19
Quinn, PJ; Markey, BK; Leonard, FC; Fitzpatrick, ES; Fanning, S and Hartigan, PJ (2011). Mycobacterium species. In: Quinn, PJ; Markey, BK; Leonard, FC; Fitzpatrick, ES; Fanning, S and Hartigan, PJ (Eds.), Veterinary microbiology and microbial disease. (2nd Edn.), Chapter 23, Oxford, Wiley-Blackwell. PP: 250-262.
20
Rogerson, BJ; Jung, YJ; LaCourse, R; Ryan, L; Enright, N and North, RJ (2006). Expression levels of Mycobacterium tuberculosis antigen encoding genes versus production levels of antigen specific T cells during stationary level lung infection in mice. Immunology. 118: 195-201.
21
Shah, DH; Verma, R; Bakshi, CS and Singh, RK (2002). A multiplex-PCR for the differentiation of Mycobacterium bovis and Mycobacterium tuberculosis. FEMS Microbiol. Lett., 214: 39-43.
22
Singh, SV; Singh, PK; Gupta, S; Chaubey, KK; Singh, B; Kumar, A; Singh, AV and Kumar, N (2013). Comparison of microscopy and blood-PCR for the diagnosis of clinical Johne’s disease in domestic ruminants. Iran. J. Vet. Res., 14: 345-349.
23
Singh, PK; Singh, SV; Kumar, H; Sohal, JS and Singh, AV (2010). Diagnostic application of IS900 PCR using blood as a source sample for the detection of Mycobacterium avium subspecies paratuberculosis in early and subclinical cases of caprine paratuberculosis. Vet. Med. Int., Article ID 748621, 8 pages.
24
Singh, SV; Singh, AV; Singh, R; Sharma, S; Shukla, N; Mishra, S; Singh, PK; Sohal, JS; Kumar, H; Patil, PK; Misra, P and Sandhu, KS (2008). Sero-prevalence of bovine Johne’s disease in buffaloes and cattle population of north India using indigenous ELISA kit based on native Mycobacterium avium subsp. paratuberculosis ‘Bison type’ genotype of goat origin. Comp. Immunol. Microbiol. Infect. Dis., 31: 419-433.
25
Siqueira, FM; Lopes, CE; Snell, GG and Gomes, MJP (2016). Identification of Mycobacterium smegmatis in bovine Mastitis. Acta Sci. Vet., (Suppl. 1), 44: 166.
26
Stott, AW; Jones, GM; Humphry, RW and Gunn, GJ (2005). Financial incentive to control paratuberculosis (Johne’s disease) on dairy farms in the United Kingdom. Vet. Rec., 156: 825-831.
27
Vary, PH; Anderson, PR; Green, E; Taylor, JH and McFadden, JJ (1990). Use of highly specific DNA probes and the polymerase chain reaction to detect Mycobacterium paratuberculosis in Johne’s disease. J. Clin. Microbiol., 28: 933-937.
28
Youssef, DGS; Sallam, FA; Darwish, SF and Amin, AS (2014). Evaluation of conventional and real-time PCR assays for molecular diagnosis of Johne’s disease in dairy cattle. Int. J. Curr. Microbiol. App. Sci., 3: 969-981.
29
Zali, MHS; Farajnia, F; Yahyapour, H; Moslemzadeh, T and Hashempour, A (2014). Detection of Mycobacterium bovis in cattle suspected to tuberculosis by PCR method in Urmia-Iran. BEPLS., 3: 152-157.
30
ORIGINAL_ARTICLE
Molecular survey of avian circoviruses in some non-psittacine birds and detection of a novel canary circovirus in a pigeon
Background: Circoviruses are small, non-enveloped, single stranded DNA viruses. There is scarce information about these agents in non-psittacine birds. Aims: It is attempted to detect and characterize circoviruses in non-psittacine birds. Methods: Forty-five samples were collected from different non-psittacine species belonging to seven avian orders. A nested polymerase chain reaction (nested-PCR) for the detection of rep gene of circoviruses was applied. Results: Two different types of circoviruses were detected in two pigeon samples (2/11, 18.2%). One of the detected circoviruses was placed in clade A next to a polish strain based on phylogenetic analysis. Interestingly, the other detected circovirus was closely related to canary circoviruses (CaCVs). Conclusion: In addition to the molecular diagnosis of a pigeon circovirus (PiCV), this is the first report of the detection of CaCv in a pigeon. The possible hypotheses of such circumstance are discussed.
https://ijvr.shirazu.ac.ir/article_5429_0a66f552d09b4fc32dea18bd4f3e77a4.pdf
2020-03-01
57
60
10.22099/ijvr.2019.34375.5078
Birds
Canary
Circovirus
Pigeon
M. R.
Haddadmarandi
1
Graduated from School of Veterinary Medicine, Shiraz University, Shiraz, Iran
AUTHOR
S. A.
Madani
madani@ut.ac.ir
2
Department of Animal and Poultry Health and Nutrition, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
LEAD_AUTHOR
H.
Nili
3
Avian Diseases Research Center, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
AUTHOR
A.
Ghorbani
4
Department of Veterinary Preventive Medicine, College of Veterinary Medicine, Ohio State University, Columbus, Ohio, America (current address)
AUTHOR
Bassami, MR; Ypelaar, I; Berryman, D; Wilcox, GE and Raidal, SR (2001). Genetic diversity of beak and feather disease virus detected in psittacine species in Australia. Virology. 279: 392-400.
1
Cságola, A; Lorincz, M; Tombácz, K; Wladár, Z; Kovács, E and Tuboly, T (2012). Genetic diversity of pigeon circovirus in Hungary. Virus Gen., 44: 75-79.
2
Dayaram, A; Goldstien, S; Zawar-Reza, P; Gomez, C; Harding, JS and Varsani, A (2013). Identification of starling circovirus in an estuarine mollusc (Amphibola crenata) in New Zealand using metagenomic approaches. Genome Announc., 1: e00278-13.
3
Goldsmith, TL (1995). Documentation of passerine circoviral infection. In Proceedings: Annual Conference of the American Association of Avian Veterinarians. Philadelphia, USA. PP: 349-350.
4
Haddadmarandi, MR; Madani, SA; Nili, H and Ghorbani, A (2018). Molecular detection and characterization of beak and feather disease virus in psittacine birds in Tehran, Iran. Iran. J. Vet. Res., 19: 22-26.
5
Halami, MY; Nieper, H; Müller, H and Johne, R (2008). Detection of a novel circovirus in mute swans (Cygnus olor) by using nested broad-spectrum PCR. Virus Res., 132: 208-212.
6
Hattermann, K; Soike, D; Grund, C and Mankertz, A (2002). A method to diagnose Pigeon circovirus infection in vivo. J. Virol. Methods. 104: 55-58.
7
Hughes, AL and Piontkivska, H (2008). Nucleotide sequence polymorphism in circoviruses. Infect. Genet. Evol., 8: 130-138.
8
ICTV Virus Taxonomy (2018). The Online (10th) Report of International Committee on Taxonomy of Viruses. Available at: https://talk.ictvonline.org/ictv-reports/ictv_ online_report.
9
Johne, R; Fernández-de-Luco, D; Höfle, U and Müller, H (2006). Genome of a novel circovirus of starlings, amplified by multiply primed rolling-circle amplification. J. Gen. Virol., 87: 1189-1195.
10
Kumar, S; Stecher, G and Tamura, K (2016). MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Mol. Biol. Evol., 33: 1870-1874.
11
Mahzounieh, M; Heidari Khoei, H; Ghasemi Shamsabadi, M and Dastjerdi, A (2014). Detection and phylogenetic characterization of Columbid circoviruses in Chaharmahal va Bakhtiari province, Iran. Avian Pathol., 43: 524-528.
12
Phenix, KV; Weston, JH; Ypelaar, I; Lavazza, A; Smyth, JA; Todd, D; Wilcox, GE and Raidal, SR (2001). Nucleotide sequence analysis of a novel circovirus of canaries and its relationship to other members of the genus Circovirus of the family Circoviridae. J. Gen. Virol., 82: 2805-2809.
13
Rampin, T; Manarolla, G; Pisoni, G; Recordati, C and Sironi, G (2006). Circovirus inclusion bodies in intestinal muscle cells of a canary. Avian Pathol., 35: 277-279.
14
Raue, R; Schmidt, V; Freick, M; Reinhardt, B; Johne, R; Kamphausen, L; Kaleta, EF; Müller, H and Krautwald-Junghanns, ME (2005). A disease complex associated with pigeon circovirus infection, young pigeon disease syndrome. Avian Pathol., 34: 418-425.
15
Sarker, S; Moylan, KG; Ghorashi, SA; Forwood, JK; Peters, A and Raidal, SR (2015). Evidence of a deep viral host switch event with beak and feather disease virus infection in rainbow bee-eaters (Merops ornatus). Sci. Rep., 5: 14511.
16
Soike, D; Hattermann, K; Albrecht, K; Segales, J; Domingo, M; Schmitt, C and Mankertz, A (2001). A diagnostic study on columbid circovirus infection. Avian Pathol., 30: 605-611.
17
Stenzel, T and Pestka, D (2014). Occurrence and genetic diversity of pigeon circovirus strains in Poland. Acta Vet. Hung., 62: 274-283.
18
Stenzel, T; Piasecki, T; Chrząstek, K; Julian, L; Muhire, BM; Golden, M; Martin, DP and Varsani, A (2014). Pigeon circoviruses display patterns of recombination, genomic secondary structure and selection similar to those of beak and feather disease viruses. J. Gen. Virol., 95: 1338-1351.
19
Tamura, K and Nei, M (1993). Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol. Biol. Evol., 10: 512-526.
20
Todd, D (2000). Circoviruses: immunosuppressive threats to avian species: a review. Avian Pathol., 29: 373-394.
21
Todd, D; Weston, J; Ball, NW; Borghmans, BJ; Smyth, JA; Gelmini, L and Lavazza, A (2001). Nucleotide sequence-based identification of a novel circovirus of canaries. Avian Pathol., 30: 321-325.
22
Zhang, Z; Dai, W; Wang, S and Dai, D (2015). Epidemiology and genetic characteristics of pigeon circovirus (PiCV) in eastern China. Arch. Virol., 160: 199-206.
23
ORIGINAL_ARTICLE
Hypertrophic osteopathy associated with aortic thrombosis in a dog
Background: Aortic thrombosis (ATh) is an uncommon problem in dogs. Although the pathogenesis of hypertrophic osteopathy (HO) is unknown, it is thought this can be due to blood flow disorder. In this case, removal of aortic thromboembolism (ATE) resulted in periosteal proliferation. Case description: A 4.8-kg, 3-year-old, intact female Maltese was referred with a bite wound showing pneumomediastinum, pneumothorax, and subcutaneous emphysema. After adequate treatment, the dog had recovered well and was discharged. Findings/treatment and outcome: Acute non-painful paraparesis suddenly developed 5 days after discharge. An abdominal ultrasound showed ATE at the level of 1 cm proximal to the external iliac arterial bifurcation. Based on clinical sign, physical exam, neurologic exam, and ultrasonography, ATh was diagnosed. Arterial thrombectomy was performed to remove the thrombus. Twenty-four days after surgery, the dog had pain and soft tissue swelling of both stifles due to HO. After rehabilitation, the dog finally started to ambulate, and the dorsal pedal arteries pulse was normal. Hypertrophic osteopathy also resolved completely. Conclusion: This report suggests the relationship between HO and ATh, for the first time in veterinary medicine.
https://ijvr.shirazu.ac.ir/article_5431_a995a4474d6d03632ea363b13f5552e8.pdf
2020-03-01
61
64
10.22099/ijvr.2019.33804.5012
Hypertrophic osteopathy
Periosteum proliferation
Thrombectomy
Thrombosis
H. S.
Cho
1
Department of Veterinary Surgery, College of Veterinary Medicine, Chonbuk National University, 79, Gobong-ro, Iksan-si, 54596, Jeollabuk-do, South Korea
AUTHOR
M. S.
Kim
minsukim@snu.ac.kr
2
Department of Veterinary Clinical Science, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, South Korea
LEAD_AUTHOR
Boswood, A; Lamb, CR and White, RN (2000). Aortic and iliac thrombosis in six dogs. J. Small Anim. Pract., 41: 109-114.
1
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ORIGINAL_ARTICLE
Multiple splenic infarctions in a dog with immune-mediated hemolytic anemia: therapeutic implications
Background: Splenic infarction (SI) is a rare clinical entity seldom encountered in veterinary medicine. Its most frequent causes include thromboembolic status, splenomegaly, and cardiac disease. Although thrombotic elements from the circulation provide the most common context for thromboembolic SIs, immune-mediated hemolytic anemia (IMHA) has not been reported as an underlying disease in canine SI. Case description: A 2-year-old, female spayed Dachshund, was referred with vomiting, hematochezia, and brown colored urine over the preceding 4 days. Physical examination revealed abnormalities including generalized weakness, jaundice, and splenomegaly; blood work showed pancytopenia and hyperbilirubinemia. Erythrocyte agglutination, polychromasia, and spherocytes on a peripheral blood smear were observed and IMHA concurrent with thrombocytopenia was diagnosed. Findings/treatment and outcome: Although erythrocyte agglutination and leukopenia disappeared after treatment, anemia and thrombocytopenia were unresponsive to oral immunosuppressive drugs and repeated transfusions. Further abdominal ultrasound identified an occlusive splenic vein thrombus. Splenic histopathology found marked multifocal to coalescing necrosis, and hemorrhage consistent with multiple SI. Symptoms resolved following splenectomy combined with 1 month of immunosuppressive medication, and the dog was healthy on follow-up evaluation after 2 years. Conclusion: Immune-mediated hemolytic anemia is an incompletely characterized cause of SI. This report establishes a potential and novel causal role for IMHA in canine SI. We believe it to be the first case report of SI in a dog with refractory IMHA and thrombocytopenia, successfully managed by splenectomy combined with short-term immunosuppressive therapy.
https://ijvr.shirazu.ac.ir/article_5419_d3e813c448661ead1fac615a764ce584.pdf
2020-03-01
65
69
10.22099/ijvr.2019.34345.5068
hemolytic anemia
splenic infarction
thrombocytopenia
thromboembolism
Venous thrombosis
S. M.
Kim
1
BSc in Veterinary Science, Department of Veterinary Internal Medicine, Konkuk University Veterinary Medical Teaching Hospital, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, South Korea
AUTHOR
G. N.
Kim
2
MSc in Veterinary Surgery, Department of Veterinary Surgery, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, South Korea
AUTHOR
S. W.
Jeong
3
Department of Veterinary Surgery, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, South Korea
AUTHOR
J. H.
Kim
junghyun@konkuk.ac.kr
4
Department of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, South Korea
LEAD_AUTHOR
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