ORIGINAL_ARTICLE
Association of oxidative status and semen characteristics with seminal plasma proteins of buffalo semen
To study the influence of season on oxidative status of buffalo semen and their association with semen characteristics and seminal plasma proteins, ejaculates were collected twice a week in winter, summer and rainy seasons from six adult Bhadawari buffalo bulls. The neat semen was analyzed for semen characteristics immediately after collection and oxidative status viz. lipid peroxidation (LPO), catalase (CAT), super oxide dismutase (SOD) activity, and total protein (TP) were estimated in seminal plasma. The protein profiling was carried out by one-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The significant effect of season was observed on TP, SOD activity and % protein fractions of seminal plasma proteins of buffalo bulls. The TP values showed positive correlation with ejaculate volume (EV), sperm concentration (SC), and % live-dead (LD) and negative correlation with progressive motility (PM), and hypo-osmotic swelling test (HOST). The SOD activity showed positive correlation with PM, LD, HOST and % acrosoamal integrity (AI). Besides that, results showed correlation of TP with 6.5, 38 and 66 kDa proteins, LPO with 70, 72, 84 and 86 kDa proteins, CAT with 70 kDa and 86 kDa proteins, and SOD with 6.5, 24.5, 44.5, 70 and 72 kDa proteins. In conclusion, this study indicated that TP and SOD activity of seminal plasma of buffalo bulls were influenced by season and were found to be associated with some of the semen characteristics and expression of seminal plasma proteins.
https://ijvr.shirazu.ac.ir/article_3906_ecc35d3f748fc76b1a4b0a544f4fb632.pdf
2016-12-01
226
230
10.22099/ijvr.2016.3906
Buffalo
Oxidative status
Season
Semen characteristics
Seminal plasma protein
L.
Sharma
1
Department of Veterinary Biochemistry, College of Veterinary Science and Animal Husbandry, Veterinary University, Mathura, 281001, India
AUTHOR
V.
Pandey
drvijaypandey@gmail.com
2
Department of Veterinary Biochemistry, College of Veterinary Science and Animal Husbandry, Veterinary University, Mathura, 281001, India
LEAD_AUTHOR
R.
Nigam
3
Department of Veterinary Biochemistry, College of Veterinary Science and Animal Husbandry, Veterinary University, Mathura, 281001, India
AUTHOR
A.
Saxena
4
Department of Veterinary Obstetrics and Gynecology, College of Veterinary Science and Animal Husbandry, Veterinary University, Mathura, 281001, India
AUTHOR
D. K.
Swain
5
Department of Veterinary Physiology, College of Veterinary Science and Animal Husbandry, Veterinary University, Mathura, 281001, India
AUTHOR
B.
Yadav
6
Department of Veterinary Physiology, College of Veterinary Science and Animal Husbandry, Veterinary University, Mathura, 281001, India
AUTHOR
Agarwal, A and Saleh, RA (2002). Role of oxidants in male infertility: rationale, significance, and treatment. Urol. Clin. North Am., 29: 817-827.
1
Alvarez, JG and Storey, BT (1989). Role of glutathione-peroxidase in protecting mammalian spermatozoa from loss of motility caused by spontaneous lipid-peroxidation. Gamete Res., 23: 77-90.
2
Atig, F; Raffa, M; Ali, HB; Abdel-hamid, K; Saad, A and Ajina, M (2012). Altered antioxidant status and increased lipid per-oxidation in seminal plasma of Tunisian infertile men. Int. J. Biol. Sci., 8: 139-149.
3
Baumber, J; Ball, BA; Gravance, CG; Medina, V and Davies-Morela, MCG (2000). The effect of reactive oxygen species on equine sperm motility, viability, acrosomal integrity, mitochondrial membrane potential and membrane lipid peroxidation. J. Androl., 21: 895-902.
4
Bergmeyer, HU; Grassl, M and Walter, HE (1983). Catalase. In: Bergmeyer, HU (Ed.), Methods of enzymatic analysis. (3rd Edn.), Vol. 2, Verlag Chemie, Weinheim. PP: 165-166.
5
Berry, EM and Kohen, R (1999). Is the biological antioxidant system integrated and regulated? Med. Hypoth., 53: 397-401.
6
Cardozo, A; Fernandez, M; Forcada, F; Abecia, A; Muino-Blanco, T and Cebrian-Perez, A (2006). Monthly varia-tion in ovine seminal plasma proteins analyzed by 2D SDS-PAGE. Theriogenology. 66: 841-850.
7
Chen, S; Chang, L and Wei, Y (2001). Oxidative damage to proteins and decrease of antioxidant capacity in patients with varicocele. Free Radic. Biol. Med., 30: 1328-1334.
8
de Souza, FF; Barreto, CS and Lopes, MD (2007). Characte-ristics of seminal plasma proteins and their correlation with canine semen analysis. Theriogenology. 68: 100-106.
9
Hammadeh, ME; Filippos, A and Hamad, MF (2009). Reactive oxygen species and antioxidant in seminal plasma and their impact on male fertility. Int. J. Fertil. Steril., 3: 87-110.
10
Hampl, R; Drabkova, P; Kandar, R and Stepan, J (2012). Impact of oxidative stress on male infertility. Ceska Gynekol., 77: 241-245.
11
Hsieh, YY; Sun, YL; Chang, CC; Lee, YS; Tsai, HD and Lin, CS (2002). Super oxide dismutase activities of spermatozoa and seminal plasma are not correlated with male infertility. J. Clin. Lab. Anal., 16: 127-131.
12
Jimenez, C; Ghyselinck, NB; Depeiges, A and Dufaure, JP (1990). Immunochemical localization and association with spermatozoa of androgen-regulated proteins of mr-24000 secreted by the mouse epididymis. Biol. Cell., 68: 171-174.
13
Kadirvel, G; Kumar, SK; Ghosh, SK and Perumal, P (2014). Activity of antioxidative enzymes in fresh and frozen thawed buffalo (Bubalus bubalis) spermatozoa in relation to lipid peroxidation and semen quality. Asian Pacif. J. Reprod., 3: 210-217.
14
Khawaskar, M; Panchal, MT; Dhami, AJ; Hadiya, KK and Patel, SB (2012). Seasonal variation in seminal bio-chemical constituents in Surti buffalo bulls. Ind. J. Anim. Reprod., 33: 41-46.
15
Kowalowka, M; Wysocki, P; Fraser, L and Strzezek, J (2007). Extracellular super oxide dismutase of boar seminal plasma. Reprod. Domes. Anim., 44: 490-496.
16
Kumar, S; Tripathi, SS and Saxena, VB (1984). A com-parative study on phosphatase, sodium and potassium in successive semen ejaculates of Red Dane, Jersey and Murrah bulls. Cheiron. 13: 136-139.
17
Laemelli, UK (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 220: 680-685.
18
Lenzi, A; Gandini, L; Maresca, V; Rago, R; Sgro, P; Dondero, F and Picardo, M (2000). Fatty acid com-position of spermatozoa and immature germ cells. Mol. Hum. Reprod., 6: 226-231.
19
Madesh, M and Balasubramanian, KA (1998). Microtiter plate assay for super-oxide dismutase using MTT reduction by super-oxide. Ind. J. Biochem. Biophy., 35: 184-188.
20
Mandal, DK; Nagpaul, PK and Gupta, AK (2000). Seasonal variation in seminal attributes and sexual behaviour of Murrah buffalo bulls. Ind. J. Dairy Sci., 53: 278-283.
21
Marti, E; Mara, L; Marti, JI; Muino-Blanco, T and Cebrian-Perez, JA (2007). Seasonal variations in anti-oxidant enzyme activity in ram seminal plasma. Theriogenology. 67: 1446-1454.
22
Nandre, RM (2008). Effect of preservation of spermatozoa at sub-zero temperature on DNA integrity by comet assay. M.V.Sc Thesis, Submitted to Anand Agricultural University. P: 63.
23
Neild, DM; Brouwers, JF; Colenbrander, B; Aguero, A and Gadella, BM (2005). Lipid peroxide formation in relation to membrane stability of fresh and frozen thawed stallion spermatozoa. Mol. Reprod. Dev., 72: 230-238.
24
Nichi, M; Bols, PEJ; Zuge, RM; Barnabe, VH; Goovaerts, IGF; Barnabe, RC and Cortada, CNM (2006). Seasonal variation in semen quality in Bos indicus and Bos taurus bulls raised under tropical conditions. Theriogenololgy. 66: 822-828.
25
Pandey, V; Nigam, R; Saxena, A; Singh, P; Sharma, A; Swain, DK and Sharma, L (2014a). Seasonal variations in biochemical attributes of Hariyana bull semen. Rumi. Sci., 3: 19-24.
26
Pandey, V; Nigam, R; Saxena, A; Singh, P; Sharma, A; Swain, DK; Sharma, L and Dixit, S (2014b). Influence of season on biochemical attributes of Bhadawari buffalo bull semen: effect of temperature and humidity. J. Anim. Res., 4: 201-209.
27
Rehman, SU (1984). Lead induced regional lipid per-oxidation in brain. Toxicol. Lett., 21: 333-337.
28
Saleh, RA and Agarwal, A (2002). Oxidative stress and male infertility: from research bench to clinical practice. J. Androl., 23: 737-752.
29
Selvaraju, S; Siva-Subramani, T; Raghavendra, BS and Ravindra, JP (2010). Effect of IGF-I on spermatozoa membrane protein profile and correlation between seminal plasma IGF-I and antioxidant enzymes in buffalo semen. Ind. J. Anim. Sci., 80: 1171-1174.
30
Shamsi, MB; Venkatesh, S; Tanwar, M; Talwar, P; Sharma, RK; Dhawan, A; Kumar, R; Gupta, NP; Malhotra, N; Singh, N; Mittal, S and Dadaa, R (2009). DNA integrity and semen quality in men with low seminal antioxidant levels. Mutat. Res., 665: 29-36.
31
Sharma, L; Pandey, V; Nigam, R; Singh, P; Saxena, A and Swain, DK (2014). Seasonal variations in seminal plasma proteins of buffalo. Reprod. Dom. Anim., 49: 387-391.
32
Shek-Vugrovecki, A; Aladrovic, J; Ljubic, BB; Laskaj, R; Majic-Balic, I and Milinkovic-Tur, S (2011). Seasonal variations in semen antioxidants of Simmental bulls. Vet. Stn. (Suppl. 1), 42: 143-148.
33
Strzezek, J; Lapkiewicz, S and Lecewicz, M (1999). A note on antioxidant capacity of boar seminal plasma. Anim. Sci. Papers Rep., 17: 181-188.
34
ORIGINAL_ARTICLE
Mammary tumor associated Hspb1 mutation and screening of eight cat populations of the world
Current research highlights the Hspb1 based screening of eight cat populations of the world to investigate the association of newly found locus within cat mammary tumors. Total 180 cats were screened on the basis of Hspb1 4 bp deletion locus (1514-1517del4) which was observed in six mammary tumor cases in Siamese cat breed. Case-control association study revealed the non-significance with P=0.201 and an overall mutant allele frequency of 0.30 ranging from 0.20-0.40 was observed in other cat populations. Similarly, HWE was also obeyed in combined population samples with P=0.860 and found non-significant with range of 0.429-0.708 in other non-Pakistani cat populations as well. These results might be helpful to understand the association of this novel locus in a better way with large sample size of cases and may also serve as a potential marker for mammary tumor diagnosis, particularly in cats and generally in all other animal populations in comparative genetics and genomics context.
https://ijvr.shirazu.ac.ir/article_3907_4e4eb87f55ce4e8cf39b232cebdce578.pdf
2016-12-01
231
236
10.22099/ijvr.2016.3907
Cat mammary lesions
Cat population
Felis catus
Hsp27 mutation
Hspb1 screening
R.
Saif
rashid.saif@vu.edu.pk
1
Department of Biotechnology, Virtual University of Pakistan, Lahore, Pakistan
LEAD_AUTHOR
A. R.
Awan
2
Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore, Pakistan
AUTHOR
L.
Lyons
3
Department of Veterinary Medicine & Surgery, College of Veterinary Medicine, University of Missouri-Columbia, Columbia, MO 65211, USA
AUTHOR
B.
Gandolfi
4
Department of Veterinary Medicine & Surgery, College of Veterinary Medicine, University of Missouri-Columbia, Columbia, MO 65211, USA
AUTHOR
M.
Tayyab
5
Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore, Pakistan
AUTHOR
M.
Ellahi Babar
6
Department of Biotechnology, Virtual University of Pakistan, Lahore, Pakistan
AUTHOR
M.
Wasim
7
Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore, Pakistan
AUTHOR
Bell, J (2008). A simple way to treat PCR products prior to sequencing using ExoSAP-IT. Biotechniques. 44: 834.
1
Burrai, GP; Mohammed, SI; Miller, MA; Marras, V; Pirino, S; Addis, MF; Uzzau, S and Antuofermo, E (2010). Spontaneous feline mammary intraepithelial lesions as a model for human estrogen receptor and progesterone receptor-negative breast lesions. BMC Cancer. 10: 156.
2
Corporation, GC (2010). Sequencher version 5.2. Sequence analysis software. Ann. Arbor Michigan.
3
Fernández, XM and Birney, E (2010). Ensembl genome browser. In: Michael Speicher, M; Antonarakis, SE and Motulsky, AG (Eds.), Vogel and Motulsky’s human genetics. (4th Edn.), New York, USA, Springer. PP: 923-939.
4
Garrido, C; Schmitt, E; Candé, C; Vahsen, N; Parcellier, A and Kroemer, G (2003). HSP27 and HSP70: potentially oncogenic apoptosis inhibitors. Cell Cycle. 2: 578-583.
5
Giménez, F; Hecht, S; Craig, LE and Legendre, AM (2010). Early detection, aggressive therapy optimizing the manage-ment of feline mammary masses. J. Feli. Medi. Surg., 12: 214-224.
6
Hughes, K and Dobson, J (2012). Prognostic histopatholo-gical and molecular markers in feline mammary neoplasia. Vet. J., 194: 19-26.
7
Knudson, AG (1971). Mutation and cancer: statistical study of retinoblastoma. Pro. Nati. Acad. Sci., 68: 820-823.
8
Lee, S; Taylor, J; Innis, M; Gelfand, D; Sninsky, J and White, T (1990). Isolation of DNA from fungal mycelia and single spores. PCR protocols, aguide to methods and applications. 2nd Edn., Orlando, Florida, Academic Press. PP: 282-287.
9
Ozcelik, H; Shi, X; Chang, MC; Tram, E; Vlasschaert, M; Di Nicola, N; Kiselova, A; Yee, D; Goldman, A and Dowar, M (2012). Long-range PCR and next-generation sequencing of BRCA1 and BRCA2 in breast cancer. J. Mol. Diagn., 14: 467-475.
10
Rozen, S and Skaletsky, H (2011). Primer3. 1998. Code available at http://www-genome wi mit edu/genome_ software/other/primer3 html.
11
Rutteman, G and Misdorp, W (1992). Hormonal background of canine and feline mammary tumours. J. Repr. Fert., Suppl., 47: 483-487.
12
Sambrook, J and Russell David, W (1989). Molecular cloning: a laboratory manual. 2nd Edn., Vol. 3, New York, USA, ColdSpringHarbor Laboratory Press. PP: 1468-1470.
13
Shafiee, R; Javanbakht, J; Atyabi, N; Bahrami, A; Kheradmand, D; Safaei, R; Khadivar, F and Hosseini, E (2013). Comparative value of clinical, cytological, and histopathological features in feline mammary gland tumors; an experimental model for the study of human breast cancer. Diagn. Pathol., 8: 1596-1598.
14
Vogelstein, B and Gillespie, D (1979). Preparative and analytical purification of DNA from agarose. Pro. Nati. Acad. Sci., 76: 615-619.
15
Zappulli, V; De Zan, G; Cardazzo, B; Bargelloni, L and Castagnaro, M (2005). Feline mammary tumours in comparative oncology. J. Dairy Res., 72: 98-106.
16
Zauber, AG; Lansdorp-Vogelaar, I; Knudsen, AB; Wilschut, J; Van Ballegooijen, M and Kuntz, KM (2008). Evaluating test strategies for colorectal cancer screening: a decision analysis for the US Preventive Services Task Force. Ann. Inter. Med., 149: 659-669.
17
ORIGINAL_ARTICLE
Expression of HA1 antigen of H5N1 influenza virus as a potent candidate for vaccine in bacterial system
The impending influenza virus pandemic requires global vaccination to prevent large-scale mortality and morbidity, but traditional influenza virus vaccine production is too slow for rapid responses. In this study, bacterial system has been developed for expression and purification of properly folded HA1 antigen as a rapid response to emerging pandemic strains. Here, a recombinant H5N1 (A/Indonesia/05/05) hemagglutinin globular domain, the synthesized HA1 (1-320 amino acids), was amplified and cloned into pET-28a bacterial expression vector. Then, his-tagged HA1 protein was expressed in Escherichia coli BL21 under 1 mM IPTG induction. The protein expression was optimized under a time-course induction study and further purified using Ni-NTA chromatography. Migration size of protein was detected at 40 KDa by Western blot using anti-His tag monoclonal antibody and demonstrated no discrepancy compared to its calculated molecular weight. Since most antigenic sites are in the HA1 domain of HA, using this domain of influenza virus as antigen is of great importance in vaccine development. The ability of the antibody stimulation against HA1 expressed in bacterial cells is also examined using enzyme-linked immunosorbent assay (ELISA) analysis. Upon immunization of rabbits, oligomeric HA1 elicited potent neutralizing antibodies and high levels of serum antibody binding to HA1. Our findings suggest that HA1-based vaccines can be produced efficiently in bacterial systems and can be easily upscaled in response to a pandemic influenza virus threat.
https://ijvr.shirazu.ac.ir/article_3908_14540320addaf8c994b014fc718fa9ff.pdf
2016-12-01
237
242
10.22099/ijvr.2016.3908
Avian influenza
Escherichia coli
HA1
Recombinant DNA
Subunit vaccine
A. S.
Farsad
1
Ph.D. Student in Plant Biotechnology, Department of Biotechnology and Plant Breeding, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
AUTHOR
S.
Malekzadeh-Shafaroudi
malekzadeh-s@um.ac.ir
2
Department of Biotechnology and Plant Breeding, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
LEAD_AUTHOR
N.
Moshtaghi
3
Department of Biotechnology and Plant Breeding, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
AUTHOR
F.
Fotouhi
4
Influenza Research Lab, Pasteur Institute of Iran, Tehran, Iran
AUTHOR
S.
Zibaee
5
Razi Vaccine and Serum Research Institute, Mashhad, Iran
AUTHOR
Biesova, Z; Miller, MA; Schneerson, R; Shiloach, J; Green, KY; Robbins, JB and Keith, JM (2009). Preparation, characterization, and immunogenicity in mice of a re-combinant influenza H5 hemagglutinin vaccine against the avian H5N1 A/Vietnam/1203/2004 influenza virus. Vaccine. 27: 6234-6238.
1
Bradford, MM (1976). A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72: 248-254.
2
Chiu, FF; Venkatesan, N; Wu, CR; Chou, AH; Chen, HW; Lian, SP; Liu, SJ; Huang, CC; Lian, WC; Chong, P and Leng, CH (2009). Immunological study of HA1 domain of hemagglutinin of influenza H5N1 virus. Biochem. Biophys. Res. Commun., 383: 27-31.
3
Dilillo, DJ; Tan, GS; Palese, P and Ravetch, JV (2014). Broadly neutralizing hemagglutinin stalk-specific anti-bodies require Fcgamma R interactions for protection against influenza virus in vivo. Nature Med., 20: 143-151.
4
Janknecht, R; de Martynoff, G; Lou, J; Hipskind, RA; Nordheim, A and Stunnenberg, HG (1991). Rapid and efficient purification of native histidine-tagged protein expressed by recombinant vaccinia virus. Proc. Natl. Acad. Sci. U. S. A., 88: 8972-8976.
5
Johansson, BE and Brett, IC (2007). Changing perspective on immunization against influenza. Vaccine. 25: 3062-3065.
6
Kanekiyo, M; Wei, CJ; Yassine, HM; McTamney, PM; Boyington, JC; Whittle, JR; Rao, SS; Kong, WP; Wang, L and Nabel, GJ (2013). Self-assembling influenza nanoparticle vaccines elicit broadly neutralizing H1N1 antibodies. Nature. 499: 102-106.
7
Khurana, S; Larkinb, C; Vermaa, S; Joshib, MB; Fontanac, J; Stevenc, AC; Kinga, LR; Manischewitza, J; McCormickb, W; Guptab, RK and Goldinga, H (2011a). Recombinant HA1 produced in E. coli forms functional oligomers and generates strain-specific SRID potency antibodies for pandemic influenza vaccines. Vaccine. 29: 5657-5665.
8
Khurana, S; Verma, S; Verma, N; Crevar, CJ; Carter, DM; Manischewitz, J; King, LR; Ross, TM and Golding, H (2010). Properly folded bacterially expressed H1N1 hemagglutinin globular head and ectodomain vaccines protect ferrets against H1N1 pandemic influenza virus. PLoS One. 5: e11548. doi: 10.1371/journal.pone. 0011548.
9
Khurana, S; Verma, S; Verma, N; Crevar, CJ; Carter, DM; Manischewitz, J; King, LR; Ross, TM and Golding, H (2011b). Bacterial HA1 vaccine against pandemic H5N1 influenza virus: evidence of oligomeriza-tion, hemagglutination, and crossprotective immunity in ferrets. J. Virol., 85: 1246-1256.
10
Lin, YJ; Deng, MC; Wu, SH; Chen, YL; Cheng, HC; Chang, CY; Lee, MS; Chien, MS and Huang, CC (2008). Baculovirus derived hemagglutinin vaccine protects chickens from lethal homologous virus H5N1 challenge. J. Vet. Med. Sci., 70: 1147-1152.
11
Olsen, B; Munster, VJ; Wallensten, A; Waldenstrom, J; Osterhaus, ADME and Fouchier, RAM (2006). Global patterns of influenza A virus in wild birds. Science. 312: 384-388.
12
Shen, S; Mahadevappa, G; Oh, HL; Wee, BY; Choi, YW; Hwang, LA; Lim, SG; Hong, W; Lal, SK and Tan, YJ (2008). Comparing the antibody responses against recombinant hemagglutinin proteins of avian influenza A (H5N1) virus expressed in insect cells and bacteria. J. Med. Virol., 80: 1972-1983.
13
Shoji, Y; Bi, H; Musiychuk, K; Rhee, A; Horsey, A; Roy, G; Green, B; Shamloul, M; Farrance, CE; Taggart, B; Mytle, N; Ugulava, N; Rabindran, S; Mett, V; Chichester, JA and Yusibov, V (2009). Plant-derived hemagglutinin protects ferrets against challenge infection with the A/Indonesia/05/05 strain of avian influenza. Vaccine. 27: 1087-1992.
14
Shoji, Y; Chichester, JA; Jones, M; Manceva, SD and Damon, E (2011). Plant based rapid production of recombinant subunit hemagglutinin vaccine targeting H1N1 and H5N1 influenza. Hum. Vaccines. 7: 41-50.
15
Stevens, J; Blixt, O; Tumpey, TM; Taubenberger, JK; Paulson, JC and Wilson, IA (2006). Structure and receptor specificity of the hemagglutinin from an H5N1 influenza virus. Science. 312: 404-410.
16
Swayne, DE and Suarez, DL (2000). Highly pathogenic avian influenza. Rev. Sci. Tech., 19: 463-482.
17
Tonegawa, K; Nobusawa, E; Nakajima, K; Kato, T; Kutsuna, T and Kuroda, K (2003). Analysis of epitope recognition of antibodies induced by DNA immunization against hemagglutinin protein of influenza A virus. Vaccine. 21: 3118-3125.
18
Treanor, JJ; Campbell, JD; Zangwill, KM; Rowe, T and Wolff, M (2006). Safety and immunogenicity of an inactivated subvirion influenza A (H5N1) vaccine. N. Engl. J. Med., 354: 1343-1351.
19
Tsai, HJ; Chi, LA and Yu, AL (2012). Monoclonal antibodies targeting the synthetic peptide corresponding to the polybasic cleavage site on H5N1 influenza hemagglutinin. J. Biomed. Sci., 19: 37-44.
20
Verma, S; Dimitrova, M; Munjal, A; Fontana, J; Crevar, CJ; Carter, DM; Ross, TM; Khurana, S and Goldinga, H (2012). Oligomeric recombinant H5 HA1 vaccine produced in bacteria protects ferrets from homologous and heterologous wild-type H5N1 influenza challenge and controls viral loads better than subunit H5N1 vaccine by eliciting high-affinity antibodies. J. Virol., 86: 12283-12293.
21
Wei, C; Nurul, T; Wahida, AG and Shaharum, S (2014). Construction and heterologous expression of a truncated haemagglutinin (HA) protein from the avian influenza virus H5N1 in Escherichia coli. Trop. Biomed., 31: 1-10.
22
ORIGINAL_ARTICLE
Allometric growth rate of the spinal cord in relation to the vertebral column during prenatal life in male and female goats (Capra hircus)
Total and regional allometric growth rates and termination sites of the spinal cord related to the respective vertebra were studied in 36 goat fetuses, from the Ahvaz slaughterhouse. These specimens were assigned to 3 groups, group 1 (CRL 10-20 cm), group 2 (CRL 21-30 cm), and group 3 (CRL 31-40 cm), each consisting of 6 male and 6 female fetuses. Observations in all 3 groups revealed that although the growth of the vertebral column was greater than that of the spinal cord, the difference in growth was not constant throughout the spine. While in cervical and thoracic regions the growth rate of the spinal cord in relation to the vertebral column was almost isometric, in the caudal part of the spine there was marked decline in growth of the spinal cord compared to the respective regions of the vertebral column. Craniocaudally, the allometric growth rate became drastically negative. There was no significant difference (P>0.05) between males and females. Except in thoracic region, all other regions showed significant differences (P<0.01) between similar regions in all 3 groups. In the lumbar region of group 2 no significant difference was found (P>0.05). As a consequence of the negative allometric growth of the spinal cord in relation to the vertebral column in the caudal part of the spine, the conus medullaris was displaced from S4-S5 in group 1 to S2 in group 3. No significant difference (P>0.05) between male and female fetuses concerning the termination of the spinal cord was found.
https://ijvr.shirazu.ac.ir/article_3909_d697f7c05a4cfb8c7f047e4e73f6aed1.pdf
2016-12-01
243
246
10.22099/ijvr.2016.3909
Allometric growth rate
Goat fetus
Termination of the spinal cord
S. M.
Ghazi
dr.s.morteza.ghazi@gmail.com
1
Ph.D. Student in Comparative Anatomy and Embryology, Department of Anatomical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
LEAD_AUTHOR
R.
Ranjbar
2
Department of Anatomical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
AUTHOR
M.
Khaksary Mahabady
3
Department of Anatomical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
AUTHOR
Barry, A (1956). A quantitative study of the prenatal changes in angulation of the spinal nerves. Anat. Res., 126: 97-110.
1
Fletcher, TF and Kitchel, RL (1966). Anatomical studies on the spinal cord segments of the dog. Am. J. Vet. Res., 27: 1759-1767.
2
Ghazi, SR and Gholami, S (1993a). Changes in the termination of spinal cord at vertebral levels during pre- and postnatal development of sheep. J. Appl. Anim. Res., 4: 61-66.
3
Ghazi, SR and Gholami, S (1993b). A study of the length of the spinal cord in pre- and postnatal life in Mehraban sheep (Ovis aries). Vet. Res. Com., 17: 417-420.
4
Ghazi, SR and Gholami, S (1994). Allometric growth of the spinal cord in relation to the vertebral column during prenatal and postnatal life in sheep (Ovis aries). J. Anat., 185: 427-431.
5
Ghazi, SR; Gholami, S and Khaksar, Z (1998). Allometric growth of the spinal cord in relation to the vertebral column during postnatal life in one humped male camel (Camelus dromedarius). J. Cam. Prac. Res., 5: 75-79.
6
Ghazi, SR; Gholami, S; Khaksar, Z; Najarzadeh, M and Gheisari, HR (2000a). Allometric growth rate of the spinal cord in relation to the vertebral column in the male and female fowl (Gallus domesticus) and turkey (Meleagris gallopavo). Iran. J. Vet. Res., 1: 39-52.
7
Ghazi, SR; Khaksar, Z and Gholami, S (2001a). Com-parative study of the allometric growth rate of the spinal cord in relation to the vertebral column in newborn male and female laboratory animals: rabbit, guinea pig and rat. Iran. J. Vet. Res., 2: 18-24.
8
Ghazi, SR; Khaksar, Z; Gholami, S and Barazandeh, M (2000b). Allometric growth rate of the spinal cord in relation to the vertebral column in rabbit. J. Fac. Vet. Med. Univ. Tehran. 55: 25-28.
9
Ghazi, SR; Khaksar, Z; Gholami, S and Dalilikajan, M (2000c). Allometric growth rate of spinal cord in relation to the vertebral column in rat. Sci. J. Fac. Vet. Med. Shahid Chamran Univ. Ahvaz. 1: 1-14.
10
Ghazi, SR; Khaksar, Z; Gholami, S and Mohammadian, N (2000d). Allometric growth of the spinal cord in relation to the vertebral column in the male and female domestic goose. J. Fac. Vet. Med. Univ. Tehran. 55: 45-48.
11
Ghazi, SR; Mansouri, SH and Ay, J (2001b). Developmental variations of the spinal cord and its termination during pre- and postnatal life in the male dog. Iran. J. Vet. Res., 2: 40-45.
12
Ghazi, SR; Mansouri, SH and Monsefi, M (2004a). The study of the developmental changes of allometric growth rate of the spinal cord in relation to the vertebral column in prenatal and postnatal male cat. Iran. J. Vet. Res., 4: 29-36.
13
Ghazi, SR; Mansouri, SH and Monsefi, M (2004b). Study of anatomical position of termination of the spinal cord in different ages of male cat. J. Fac. Vet. Med. Univ. Tehran. 59: 199-200.
14
Gholami, S; Ghazi, SR and Khaksar, Z (1997). Postnatal changes of termination of the spinal cord in camel (Camelus dromedarius). J. Appl. Anim. Res., 11: 69-72.
15
Hifny, A; Ahmed, AK and AMnsour, AA (1984). The relation between the vertebral column and spinal cord of Equus asinus. Assiut. Vet. Med. J., 12: 3-6.
16
Khaksar, Z; Ghazi, SR and Gholami, S (2002). Quantitative study of the vertebral column and spinal cord in adult male and female pigeons. J. Fac. Vet. Med. Univ. Tehran. 57: 39-42.
17
Kunitomo, K (1918). The development and reduction of the tail and of the caudal end of the spinal cord. Contr. Embryol., 8: 161-198.
18
Malinska, J; Kapoun, S and Malinski, J (1972). Topography of the spinal cord in the east central European hedgehog. Folia Morphol. (Prague). 20: 182-184.
19
McCotter, RE (1916). Regarding the length and extent of the human medulla spinalis. Anat. Res., 10: 559-564.
20
Morgan, JP; Atilola, M and Bailey, CS (1987). Vertebral canal and spinal cord mensuration: a comparative study of its effect on lumbosacral myelography in the Dachshund and German shepherd dog. JAVMA. 191: 951-957.
21
Nizankowski, C and Kurlej, W (1982). Contribution to studies on the apparent ascent of the spinal cord in human fetuses. Folia Morphol. (Warsz). 41: 33-48.
22
Rahmanifar, F; Ghazi, SR and Mansouri, SH (2008). A comparative study of allometric growth rate of spinal cord in relation to the vertebral column in chick and adult male ostrich (Struthio camelus). Sci. J. Fac. Vet. Med. Shahid Chamran Univ. Ahvaz. 3: 39-47.
23
Roth, ML and Purkyne, JE (1985). Normal neurovertebral growth relation. Acta Fac. Med. Univ. Brun., Opusc Morphol., 91: 11-34.
24
Sakla, FB (1969). Quantitative studies on the postnatal growth of the spinal cord and the vertebral column of the Albino mouse. J. Com. Neurosci., 136: 237-252.
25
Streeter, GL (1919). Factors involved in the formation of the filum terminal. Am. J. Anat., 25: 1-11.
26
Taluja, JS; Shrivastava, A and Malik, MR (1989). Regional cross-sectional area of the prenatal caprine spinal ganglia. Ind. J. Anim. Sci., 59: 829-830.
27
ORIGINAL_ARTICLE
Study on follicular characteristics, hormonal and biochemical profile in norgestomet+PMSG treated acyclic buffaloes
This research was conducted to study the follicular dynamics, hormonal, biochemical profile and fertility response in acyclic and norgestomet+PMSG treated acyclic buffaloes in summer. The study animals were divided into two groups: group I [control (n=8): no treatment] and II [treatment group (n=15)]. In group II, seven animals were used for follicular biochemical and hormonal profile and eight animals for fertility studies following Crestar® (Intervet, France) treatment (day 0: Crestar® insertion; day 8: 500 IU PMSG; day 9: Crestar® removal; day 11 AI). Follicular fluid stradiol (E2) and progesterone (P4) in acyclic and pre-ovulatory follicle in study groups was significantly (P<0.01) higher than peripheral level. Peripheral E2 concentration, during pre-ovulatory period in group II was higher (P<0.05) than group I. Significant correlation between serum and follicular E2 was deduced (r=0.888; P<0.01) as significant difference in serum cholesterol content was shown between groups. Lower follicular total protein (P<0.05) in acyclic animals and higher follicular glucose (P<0.05) in treated group were concluded. Significant correlation (r=-0.770; P<0.05) was observed between follicular cholesterol and triglycerides. Follicular characteristics, post PMSG administration, differed significantly (0.83 ± 0.20 vs 1.32 ± 0.12; P<0.01) in all buffaloes exhibiting estrus, out of which four conceived. In conclusion, follicular hormonal and biochemical profile exhibits alteration in protein and glucose level between summer acyclic and treated buffaloes. However, peripheral E2 along with fertility response showed significant difference (P<0.01) between the study groups with significant correlation in E2, cholesterol and triglycerides between peripheral and follicular compartment.
https://ijvr.shirazu.ac.ir/article_3910_1b3185c5ccf77de6ba2190ccefcf9d21.pdf
2016-12-01
247
252
10.22099/ijvr.2016.3910
Acyclicity
Buffalo
Follicular dynamics
Norgestomet
PMSG
A.
Jerome
jerome210982@gmail.com
1
Animal Physiology and Reproduction Division, ICAR-Central Institute for Research on Buffaloes, Haryana, 125001, India
LEAD_AUTHOR
S. K.
Srivastava
2
Animal Reproduction Division, ICAR-Indian Veterinary Research Institute, Bareilly, Uttar Pradesh, 243122, India
AUTHOR
R. K.
Sharma
3
Animal Physiology and Reproduction Division, ICAR-Central Institute for Research on Buffaloes, Haryana, 125001, India
AUTHOR
Abdellah, MR; Hussein, HA and Derar, DR (2010). Ovarian follicular fluid constituents in relation to stage of estrous cycle and size of the follicle in buffalo. Vet. World. 3: 263-267.
1
Alkalby, JMA; Bushra, FH and Fahad, TA (2012). Study on some hormonal and biochemical constituents of follicular fluid and blood plasma in buffaloes. Bas. J. Vet. Res., 11: 90-102.
2
Arshad, HM; Ahmad, N; Zia-ur-Rahman; Samad, HA; Akhtar, N and Ali, S (2005). Studies on some biochemical constituents of ovarian follicular fluid and peripheral blood in buffaloes. Pakistan Vet. J., 25: 189-193.
3
Badinga, L; Thatcher, WW; Diaz, T; Drost, M and Wolfenson, D (1993). Effect of environmental heat-stress on follicular development and steroidogenesis in lactating Holstein cows. Theriogenology. 39: 797-810.
4
Baki Acar, D; Birdane, MK; Dogan, N and Gurler, H (2013). Effect of the stage of estrous cycle on follicular population, oocyte yield and quality, and biochemical composition of serum and follicular fluid in Anatolian water buffalo. Anim. Reprod. Sci., 137: 8-14.
5
Barile, VL (2005). Improving reproductive efficiency in female buffaloes. Livest. Prod. Sci., 92: 83-194.
6
Barile, VL; Gallaso, A; Carretta, A; Marchiori, E and Borghese, A (2001). Evaluation of different timed inseminations on conception rate in synchronized Italian buffaloes. In: Proceedings of Sixth World Buffalo Congress, Venezuela. PP: 172-178.
7
Baruselli, PS; Reis, EL; Marques, MO; Nasser, LF and Bo, GA (2004). The use of hormonal treatments to improve reproductive performance of anestrus beef cattle in tropical climates. Anim. Reprod. Sci., 82-83: 479-486.
8
Borghese, A (2005). In: Technical Series 67. Food and Agriculture Organization, Rome, Italy.
9
Cerri, RLA; Rutigliano, HM; Bruno, RGS and Santos, JEP (2009). Progesterone concentration, follicular development and induction of cyclicity in dairy cows receiving intra-vaginal progesterone inserts. Anim. Reprod. Sci., 110: 56-70.
10
Chaudhari, CF; Suthar, BN; Sharma, VK; Dabas, VS; Chaudhari, NF and Panchasara, HH (2012). Estrus induction and fertility response in delayed pubertal Kankrej heifers treated with norgestomet ear implant. Vet. World. 5: 453-458.
11
Chaudhary, JK; Khasatiya, CT; Parmar, SC; Patel, RV and Patel, MD (2015). Serum progesterone and oestradiol-17β profile in norgestomet primed postpartum silent estrus surti buffaloes. Wayamba J. Anim. Sci., Article No. 1427620266: 1095-1103.
12
Das, GK and Khan, FA (2010). Summer anoestrus in buffalo - a review. Reprod. Domes. Anim., 45: 483-494.
13
De Rensis, F and Lopez-Gatius, F (2007). Protocols for synchronizing estrus and ovulation in buffalo (Bubalus bubalis): a review. Theriogenology. 67: 209-216.
14
De Rensis, F and Scaramuzzi, RJ (2003). Heat stress and seasonal effects on reproduction in the dairy cow. A review. Theriogenology. 60: 1139-1151.
15
Dodamani, MS; Tandle, MK; Mohteshamuddin, K and Honnappagol, SS (2011). Induction of fertile estrus in true anoestrus by re-utilization of Crestar implants in She buffaloes. Vet. World. 4: 28-30.
16
Edmonson, AJ; Lean, IJ; Weaver, LD; Farver, T and Webster, G (1989). A body condition scoring chart for Holstein dairy cows. J. Dairy Sci., 72: 68-78.
17
El-Fadaly, MA; Atiefa, AA; Abass, HI; El-Essawy, GS and Essawy, SA (1994). Induction of cyclicity in anestrus and subestrus postpartum Egyptian buffaloes. Proceedings of the 4th World Buffalo Congress. Sao Paulo, Brazil. PP: 539-551.
18
El-Shahat, KH and Kandil, M (2012). Antioxidant capacity of follicular fluid in relation to follicular size and stage of estrous cycle in buffaloes. Theriogenology. 77: 1513-1518.
19
Fu, SB; Zhang, HL; Riaz, H; Ahmad, S; Wang, XM; Li, X; Hua, GH; Liu, XR; Guo, AZ and Yang, LG (2013). Effects of different doses of PMSG on reproductive performance in Chinese Holstein dairy cows. Pak. Vet. J., 33: 209-212.
20
Garcia-Winder, M; Lewis, PE; Townsend, EC; Lewis, GS and Inskeep, EK (1987). Effects of norgestomet on follicular development in postpartum beef cows. J. Anim. Sci., 64: 1099-1109.
21
Khan, FA; Das, GK; Pande, M; Mir, RA and Shankar, U (2011). Changes in biochemical composition of follicular fluid during reproductive acyclicity in water buffalo (Bubalus bubalis). Anim. Reprod. Sci., 127: 38-42.
22
Khan, FA; Das, GK; Pande, M; Sarkar, M; Mahapatra, RK and Shankar, U (2012). Alterations in follicular fluid estradiol, progesterone and insulin concentrations during ovarian acyclicity in water buffalo (Bubalus bubalis). Anim. Reprod. Sci., 130: 27-32.
23
Khodaei-Motlagh, M; Zare Shahneh, A; Masoumi, R and De Rensis, F (2011). Alterations in reproductive hormones during heat stress in dairy cattle. African J. Biotech., 10: 5552-5558.
24
Kumar, S; Balhara, AK; Kumar, R; Kumar, N; Buragohain, L; Baro, D; Sharma, RK; Phulia, SK and Singh, I (2015). Hemato-biochemical and hormonal profiles in post-partum water buffaloes (Bubalus bubalis). Vet. World. 8: 512-517.
25
Kumar, H; Bhooshan, N; Patra, MK and Yadav, MC (2010). Treatment with progestagen and PMSG to prevent prolonged anestrus in buffaloes. Indian J. Anim. Sci., 80: 623-625.
26
Leroy, JLMR; Vanholder, T; Delanghane, JR; Opsomer, G; Van-Soom, A; Bols, PEJ and DeKruif, A (2004). Metabolite and ionic composition of follicular fluid from different-sized follicles and their relationship to serum concentrations in dairy cows. Anim. Reprod. Sci., 80: 201-211.
27
Lohan, IS; Saini, MS; Kaker, ML; Malik, RK; Singh, B and Grewal, SS (2001). Follicular changes in postpartum buffaloes (Bubalus bubalis) induced to cyclicity. Indian J. Anim. Sci., 71: 918-921.
28
Malik, RK; Singh, P; Sharma, RK; Singh, I; Phulia, SK and Tuli, RK (2011). Efficacy of norgestomet ear implant for estrus induction on postpartum anestrus Murrah buffaloes (Bubalus bubalis). Indian J. Anim. Sci., 81: 687-690.
29
Malik, RK; Singh, P; Sharma, RK; Singh, I and Tuli, RK (2010). Estrus and fertility response of postpartum anestrus Murrah buffaloes to Crestar and Ovsynch treatment regimens. Indian J. Anim. Sci., 80: 982-985.
30
Marai, IFM and Haeeb, AAM (2010). Buffalo’s biological functions as affected by heat stress-A review. Livestock Sci., 127: 89-109.
31
Nayak, V; Agarwal, RG; Srivastav, OP and Thakur, MS (2009). Induction of oestrus in true anestrus buffaloes using Crestar implant alone and in combination with PMSG. Buffalo Bull., 28: 51-54.
32
Neglia, G; Gasparrini, B; Palo, RD; Rosa, CD; Zicarelli, L and Campanile, G (2003). Comparison of pregnancy rates with two estrus synchronization protocols in Italian Mediterranean Buffalo cows. Theriogenology. 60: 125-133.
33
Ozyurtlu, N; Cetin, Y; Kicukaslan, I and Kocamuftuoglu, M (2009). Induction of oestrus with norgestomet ear implant and PRID in acyclic holstein heifers. J. Anim. Vet. Adv., 8: 1035-1039.
34
Pandey, AK; Dhaliwal, GS; Ghuman, SS; Singh, J; Kumar, A and Agarwal, SK (2013). Impact of norgestomet supplementation during early luteal phase on subsequent luteal profiles and conception rate in buffalo: a preliminary study. Trop. Anim. Health Prod., 45: 293-298.
35
Pieterse, MC; Kappen, KA; Kruip, TA and Taverne, MA (1988). Aspiration of bovine oocytes during transvaginal ultrasound scanning of the ovaries. Theriogenology. 30: 751-762.
36
Qureshi, MS (2009). Nutritional and management support to reproduction in dairy buffaloes under tropical conditions. Pakistan J. Zool. Suppl. Ser., 9: 895-909.
37
Qureshi, MS; Habib, G; Nawab, G; Siddiqui, MM; Ahmad, N and Samad, HA (2000). Milk progesterone profiles in various reproductive states in dairy buffaloes under field conditions. Proceedings of National Science Council. Taipei, Taiwan. 24: 70-75.
38
Qureshi, MS; Habib, G; Samad, HA; Siddiqui, MM; Ahmad, N and Syed, M (2002). Reproduction-nutrition relationship in dairy buffaloes. I. Effect of intake of protein, energy and blood metabolites levels. Asian-Aust. J. Anim. Sci., 15: 330-339.
39
Rao, LV and Pandey, RS (1982). Seasonal changes in the plasma progesterone concentration in buffalo cow (Bubalus bubalis). J. Reprod. Fertil., 66: 57-61.
40
Rohilla, N; Singh, U; Sharma, RK and Singh, I (2005). Ultrasonic ovarian status in summer anestrus postpartum Murrah buffaloes. Indian J. Anim. Reprod., 26: 95-98.
41
Roth, Z; Arav, A; Bor, A; Zeron, Y; Braw-Tal, R and Wolfenson, D (2001). Improvement of quality of oocytes collected in the autumn by enhanced removal of impaired follicles from previously heat-stressed cows. Reproduction. 122: 737-744.
42
Sharma, RK; Singh, JK; Khanna, S and Singh, I (2012). Ovarian response of prepubertal Murrah heifers to exogenous GnRH. Anim. Reprod. Sci., 133: 153-158.
43
Singh, C (2003). Response of anoestrus rural buffaloes (Bubalus bubalis) to intravaginal progesterone implant and PGF2a injection in summer. J. Vet. Sci., 4: 137-141.
44
Singh, AS; Saxena, MS and Prasad, S (2004). Efficacy of Crestar and its combination with folligon on postpartum anoestrus in buffaloes. Indian J. Anim. Reprod., 25: 43-44.
45
Singh, G; Singh, GB and Dhaliwal, GS (1989). Studies on reproductive status of rural buffaloes in summer. Indian J. Anim. Reprod., 10: 151-153.
46
Snedecor, GW and Cochran, WG (1989). Statistical methods. 8th Edn., Ames, IowaStateUniversity Press. P: 511.
47
Thangapandiyan, M; Pothiappan, P; Palaniappan, RM; Joseph, ES and Kathiresan, D (2015). Induction of estrus in anestrus murrah buffaloes and programmed breeding. Buffalo Bull., 34: 241-244.
48
Thangavel, N (2004). Studies on certain biochemical profile of the buffalo follicular fluid. Indian Vet. J., 81: 25-27.
49
Varughese, EE; Brar, PS; Honparkhe, M and Ghuman, SPS (2014). Correlation of blood flow of the pre-ovulatory follicle to its diameter and endocrine profile in dairy buffalo. Reprod. Dom. Anim., 49: 140-144.
50
Wiltbank, MC; Souza, AH; Carvalho, PD; Bender, RW and Nascimento, AB (2011). Improving fertility to timed artificial insemination by manipulation of circulating progesterone concentrations in lactating dairy cattle. Reprod. Fertil. Dev., 24: 238-243.
51
Zicarelli, L (1997). Reproductive seasonality in buffalo. Bubalus Bubalis. 4: 52-54.
52
ORIGINAL_ARTICLE
Developmental competence of Dromedary camel oocytes fertilized in vitro by frozen-thawed ejaculated and epididymal spermatozoa
The present study aimed to compare the in vitro fertilizing capacity of frozen-thawed ejaculated and epididymal spermatozoa in order to standardize the semen preparation protocol for camel in vitro fertilization (IVF). Semen samples were collected from 7 Dromedary camels by means of artificial vagina (AV). Ten cauda epididymes were obtained from slaughtered adult camels, isolated, incised and rinsed for obtaining the sperm rich fluid. Ejaculated and epididymal spermatozoa were processed for cryopreservation. Fresh and frozen-thawed ejaculated and epididymal spermatozoa were evaluated for motility, livability, membrane and acrosomal integrities. Frozen-thawed ejaculated and epididymal spermatozoa were used to fertilize camel mature oocytes in vitro. The results showed that, the progressive motility of freshly collected epididymal spermatozoa was significantly (P<0.05) higher than ejaculated spermatozoa (49.25 ± 1.75 vs. 38.50 ± 1.50%, respectively). The viability index of epididymal spermatozoa was significantly (P<0.05) higher than that of ejaculated spermatozoa (96.63 ± 2.45 vs. 84.00 ± 4.08, respectively). The post-thaw acrosome and membrane integrities of epididymal spermatozoa were significantly (P<0.05) higher than those of ejaculated spermatozoa. Morula and blastocyst rates of camel oocytes in vitro fertilized by frozen-thawed epididymal spermatozoa (59.4 ± 0.8, 19.12 ± 0.7 and 10.29 ± 0.7%, respectively) were significantly (P<0.05) higher than those fertilized by frozen-thawed ejaculated spermatozoa (48.27 ± 3.1, 11.63 ± 1.1 and 5.43 ± 0.8%, respectively). In conclusion, the Dromedary camel frozen epididymal spermatozoa have the capacity to endure cryopreservation, fertilize oocytes and produce embryos in vitro better than ejaculated sperm.
https://ijvr.shirazu.ac.ir/article_3911_6fa4455276c25ff7a53b5bb018d36c7f.pdf
2016-12-01
253
258
10.22099/ijvr.2016.3911
Camel
Ejaculated semen
Epididymal spermatozoa
In vitro fertilization
T. H.
Scholkamy
1
Department of Field Investigations, Animal Reproduction Research Institute, Agriculture Research Center, Giza, Egypt
AUTHOR
D. A.
El-Badry
2
Department of Artificial Insemination and Embryo Transfer, Animal Reproduction Research Institute, Agriculture Research Center, Giza, Egypt
AUTHOR
K. Gh. M.
Mahmoud
karimamahmoud@yahoo.com
3
Department of Animal Reproduction and Artificial Insemination, National Research Center, Dokki, Giza, Egypt
LEAD_AUTHOR
Abdoon, AS; Kandil, OM; Berisha, B; Kliem, H and Schams, D (2007). Morphology of Dromedary camel oocytes and their ability to spontaneous and chemical parthenogenetic activation. Reprod. Dom. Anim., 42: 88-93.
1
Abdoon, AS; Kandil, OM; Pizzi, F; Turri, F; El Atrash, A and Sabra, HA (2013). Effect of semen extender on cryopreservation and fertilization rates of Dromedary camel epididymal spermatozoa. Scientific Conference of Camel Research and Production. Khartoum, Sudan, 17th-18th April 2013.
2
Al-Qarawi, HA; Abdel-Rahman, SA; El-Mougy, MS and El-Belely, AA (2002). Use of a new computerized system for evaluation of spermatozoal motility and velocity characteristics in relation to fertility levels in Dromedary bulls. Anim. Reprod. Sci., 74: 1-9.
3
Axner, E; Hermansson, U and Linde-Forsberg, C (2004). The effect of Equex STM paste and sperm morphology on post-thaw survival of cat epididymal spermatozoa. Anim. Reprod. Sci., 84: 179-191.
4
Badr, MR and Abdel-Malak, MG (2010). In vitro fertilization and embryo production in Dromedary camel using epididymal spermatozoa. Global Vet., 4: 271-276.
5
Barker, CA and Gandier, SCC (1957). Pregnancy in a mare resulting from frozen epididymal spermatozoa. Can. J. Comp. Med., 21: 47-51.
6
Billah, M and Skidmore, JA (1992). The collection, evaluation and deep-freezing of Dromedary camel semen. In: Proceedings of 1st International Camel Conference. Dubai, UAE, February 2-6. P: 61 (abst.).
7
Blash, S; Melican, D and Gavin, W (2000). Cryopreservation of epididymal sperm obtained at necropsy from goats. Theriogenology. 54: 899-905.
8
Brackett, BG and Oliphant, G (1975). Capacitation of rabbit spermatozoa in vitro. Biol. Reprod. 12: 260-274.
9
Chaveiro, A; Cerqueira, C; Silva, J; Franco, J and Moreira da Silva, F (2015). Evaluation of frozen thawed cauda epididymal sperms and in vitro fertilizing potential of bovine sperm collected from the cauda epididymal. Iran. J. Vet. Res., 16: 188-193.
10
Didion, BA; Dobrinsky, JR; Giles, JR and Graves, CN (1989). Staining procedure to detect viability and true acrosome reaction in spermatozoa of various species. Gamete Res., 22: 51-57.
11
El-Badry, DA; Scholkamy, TH; Anwer, AM and Mahmoud, KGhM (2015). Assessment of freezability and functional integrity of Dromedary camel spermatozoa harvested from caput, corpus and cauda epididymides. Alexandria J. Vet. Sci., 44: 147-158.
12
El-Sayed, A; Ashour, G; Kamel, AM and El-Bahrawy, KA (2015). Assessment of embryo production of Dromedary (Camelus dromedarius) using two semen sources and two in vitro fertilization techniques. Egypt. J. Anim. Prod., 52: 153-160.
13
El-Sayed, A; Sayed, HA; El-Hassanein, EE; Murad, H and Barkawi, AH (2012). Effect of epidermal growth factor on in vitro production of camel (Camelus dromedarius) embryos by using frozen semen. Egypt. J. Anim. Prod., 49: 39-45.
14
Fathi, M; Seida, AA; Sobhy, RR; Darwish, GM; Badr, MR and Moawad, AR (2014). Caffeine supplementation during IVM improves frequencies of nuclear maturation and preimplantation development of Dromedary camel oocytes following IVF. Theriogenology. 81: 1286-1292.
15
García-Álvarez, O; Maroto-Morales, A; Martínez-Pastor, F; Garde, JJ; Ramón, M; Fernández-Santos, MR; Esteso, MC; Pérez-Guzmán, MD and Soler, AJ (2009). Sperm characteristics and in vitro fertilization ability of thawed spermatozoa from Black Manchega ram: electro-ejaculation and postmortem collection. Theriogenology. 72: 160-168.
16
Hassan, MM; Saed, M and Muqtadir, R (1995). Semen collection by artificial vagina and cryopreservation of camel (Camelus dromederious) spermatozoa. Pak. Vet. J., 15: 105-108.
17
Heise, A; Thompson, PN and Gerber, D (2011). Influence of seminal plasma on fresh and post-thaw parameters of stallion epididymal spermatozoa. Anim. Reprod. Sci., 123: 192-201.
18
Jeyendran, RS; Vander-Ven, HH; Perez-Pelaez, M; Crabo, BG and Zanevld, LJD (1984). Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characters. J. Repord. Fertil., 70: 219-228.
19
Kaabi, M; Paz, P; Alvarez, M; Anel, E; Boixo, JC; Rouissi, H; Herraez, P and Anel, L (2003). Effect of epididymis handling conditions on the quality of ram spermatozoa recovered post-mortem. Theriogenology. 60: 1249-1259.
20
Katska, L; Ryńska, B and Smora, Z (1996). Effect of seminal plasma on the in vitro fertilizability of bull spermatozoa. Anim. Reprod. Sci., 44: 23-31.
21
Khatir, H and Anouassi, A (2006). The first Dromedary (Camelus dromedarius) offspring obtained from in vitro matured, in vitro fertilized and in vitro cultured abattoir-derived oocytes. Theriogenology. 65: 1727-1736.
22
Khatir, H; Anouassi, A and Tibary, A (2007). Quality and developmental ability of Dromedary (Camelus dromeda-rius) embryos obtained by IVM/IVF, in vivo matured/IVF or in vivo matured/fertilized oocytes. Reprod. Dom. Anim., 42: 263-270.
23
Lambrechts, H; van Niekerk, FE; Coetzer, WA; Cloete, SWP and van der Horst, G (1999). The effect of cryopreservation on the survivability, viability and motility of epididymal African buffalo (Syncerus caffer) spermato-zoa. Theriogenology. 52: 1241-1249.
24
Martins, CF; Rumpf, R; Pereira, DC and Dode, MN (2007). Cryopreservation of epididymal bovine spermatozoa from dead animals and its uses in vitro embryo production. Anim. Reprod. Sci., 101: 326-331.
25
Moawad, AR; Darwish, GM; Badr, MR and El-Wishy, AB (2011). In vitro fertilization of Dromedary camel (Camelus dromedaries) oocytes with epididymal spermatozoa. Reprod. Fertil. Dev., 24: 192-193.
26
Monteiro, GA; Papa, FO; Zahn, FS; Dellaqua Jr, JA; Melo, CM; Maziero, RRD; Avanzi, BR; Alvarenga, MA and Guasti, PN (2011). Cryopreservation and fertility of ejaculated and epididymal stallion sperm. Anim. Reprod. Sci., 127: 197-201.
27
Niasari-Naslaji, A; Mosaferi, S; Bahmani, N; Gerami, A; Gharahdaghi, AA; Abarghani, A and Ghanbari, A (2007). Semen cryopreservation in Bactrian camel (Camelus bactrianus) using Shotor diluent: effects of cooling rates and glycerol concentrations. Theriogenology. 68: 618-625.
28
Niwa, K and Ohgoda, O (1988). Synergistic effect of caffeine and heparin on in vitro fertilization of cattle oocytes matured in culture. Theriogenology. 30: 733-741.
29
Nowshari, MA and Wani, NA (2005). Camelid embryo development in vitro: effect of protein supplementation in maturation medium and subsequent culture in two different media on fertilization and development. Reprod. Fertil. Dev., 17: 276-282.
30
Rath, D and Niemann, H (1997). In vitro fertilization of porcine oocytes with fresh and frozen-thawed ejaculated or frozen-thawed epididymal semen obtained from identical boars. Theriogenology. 47: 785-793.
31
Reyes-Moreno, C; Boilard, M; Sullivan, R and Sirard, M (2002). Characterization and identification of epididymal factors that protect ejaculated bovine sperm during in vitro storage. Biol. Reprod., 66: 159-166.
32
Sieme, I; Merkt, H; Musa, B; Badreldin, H and Willmen, T (1990). Liquid and deep-freezing preservation of camel semenusing different extenders and methods. In: Proceedings of the workshop “Is it possible to improve the reproductive performance of the camel?”, Paris, September 10-12, 1990. PP: 273-284.
33
Snedecor, GW and Cochran, WG (1989). Statistical methods. 8th Edn., Ames, IA, USA, Iowa State University Press. P: 97.
34
Turri, F; Kandil, OM; Abdoon, AS; Sabra, H; El Atrash, A and Pizzi, F (2013). Conservation of camel genetic resources: epididymal sperm recovery. The Camel Conference@ SOAS. 29 April 2013. PP: 27-32.
35
Turri, F; Madeddu, M; Gliozzi, TM; Gandini, G and Pizzi, F (2014). Effect of testicle postmortem storage on goat frozen-thawed epididymal sperm quality as a tool to improve gene banking in local breeds. Int. J. Anim. Biosci., 8: 440-447.
36
Varisli, O; Uguz, C; Agca, C and Agca, A (2009). Motility and acrosomal integrity comparisons between electro-ejaculated and epididymal ram sperm after exposure to a range of an isosmotic solutions, cryoprotective agents and low temperatures. Anim. Reprod. Sci., 110: 256-268.
37
Wakayama, T and Yanagimachi, R (1998). Development of normal mice from oocytes injected with freeze-dried spermatozoa. Nat. Biotechnol., 16: 639-646.
38
Wani, NA (2009). In vitro embryo production in camel (Camelus dromedarius) from in vitro matured oocytes fertilized with epididymal spermatozoa stored at 4°C. Anim. Reprod. Sci., 111: 69-79.
39
Wani, NA; Billah, M and Skidmore, JA (2008). Studies on liquefaction and storage of ejaculated Dromedary camel (Camelus dromedarius) semen. Anim. Reprod. Sci., 109: 309-318.
40
Wani, N; Nowshari, M and Wernery, U (2005). Storage of camel (Camelus dromedarius) epididymal spermatozoa in different extenders and at different temperatures. 38th Annu. Meet. Soc. Stud. Reprod., Quebec, Canada, W693.
41
Wani, NA and Wernery, U (2010). In vitro maturation of Dromedary (Camelus dromedarius) oocytes: effect of different protein supplementations and epidermal growth factor. Reprod. Dom. Anim., 45: e189-e193.
42
Yu, I and Leibo, SP (2002). Recovery of motile, membrane-intact spermatozoa from canine epididymides stored for 8 days at 4°C. Theriogenology. 57: 1179-1190.
43
Ziapour, S; Niasari-Naslaji, A; Mirtavousi, M; Keshavarz, M; Kalantari, A and Adel, H (2014). Semen collection using phantom in Dromedary. Anim. Reprod. Sci., 151: 15-21.
44
ORIGINAL_ARTICLE
Neuropharmacological properties of farnesol in Murine model
Research on new compounds of therapeutic value for behavioral disorders has progressed recently. Several studies have reported neuropharmacological activities of plant derived terpenes. Farnesol is a sesquiterpene whose most popular source is fruits but the anxiolytic activity for farnesol is still unknown. The present study was conducted on 32 male Swiss Albino mice (8 in each group) to evaluate the neuropharmacological properties of farnesol and its effects on plasma cortisol levels. Farnesol was administered intraperitoneally at single doses of 50 and 100 mg/kg, while diazepam 2 mg/kg was used as standard anxiolytic. Thirty minutes after injections, open field test (OFT), elevated plus maze (EPM), a forced swimming test (FST), and a hot plate test (HPT) were performed for evaluation of anxiety-like behavior, depression and nociception. In OFT, farnesol at the dose of 100 mg/kg led to significant decrease in locomotor activity (P<0.01). In EPM, only farnesol 100 mg/kg led to significant increase in the number of entries to the open arms and the time spent in open arms (P<0.01). Increase in immobility time in FST was seen in farnesol 50 and 100 mg/kg (P<0.001). Farnesol 100 mg/kg exerts significant prolongation in the latency of responses to noxious heat stimuli in HPT. Like diazepam, farnesol decreased plasma levels of cortisol. Results revealed that farnesol had anxiolytic, anti-nociceptive and depressant effects in murine models. The present study provides pharmacological evidence supporting the use of farnesol as a sedative for anxiety disorders.
https://ijvr.shirazu.ac.ir/article_3912_3687fefa6c15643c478af8848da8584b.pdf
2016-12-01
259
264
10.22099/ijvr.2016.3912
Anxiolytic
Anti-nociceptive
Cortisol
Depressive behavior
Farnesol
M.
Shahnouri
1
Faculty of Veterinary Medicine, Babol Branch, Islamic Azad University, Babol, Iran
AUTHOR
M.
Abouhosseini Tabari
m.abouhosseini@ausmt.ac.ir
2
Department of Pharmacology, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran
LEAD_AUTHOR
A.
Araghi
3
Department of Clinical Pathology, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran
AUTHOR
Bradley, BF; Starkey, NJ; Brown, SL and Lea, RW (2007). Anxiolytic effects of Lavandula angustifolia odour on the Mongolian gerbil elevated plus maze. J. Ethnopharmacol., 111: 517-525.
1
Casarrubea, M; Sorbera, F; Santangelo, A and Crescimanno, G (2012). The effects of diazepam on the behavioral structure of the rat’s response to pain in the hot-plate test: anxiolysis vs. pain modulation. Neuropharma-cology. 63: 310-321.
2
Costa, JP; de Oliveira, GAL; de Almeida, AAC; Islam, MT; de Sousa, DP and de Freitas, RM (2014). Anxiolytic-like effects of phytol: possible involvement of GABAergic transmission. Brain Res., 1547: 34-42.
3
Costa, CARA; Kohn, DO; de Lima, VM; Gargano, AC; Flório, JC and Costa, M (2011). The GABAergic system contributes to the anxiolytic-like effect of essential oil from Cymbopogon citratus (lemongrass). J. Ethnopharmacol., 137: 828-836.
4
Couto, VM; Vilela, FC; Dias, DF; dos Santos, MH; Soncini, R; Nascimento, CGO and GiustiPaiva, A (2011). Antinociceptive effect of extract of Emilia sonchifolia in mice. J. Ethnopharmacol., 134: 348-353.
5
De Almeida, AAC; Costa, JP; de Carvalho, RBF; de Sousa, DP and de Freitas, RM (2012). Evaluation of acute toxicity of a natural compound (+)-limonene epoxide and its anxiolytic-like action. Brain Res., 1448: 56-62.
6
de Oliveira Júnior, WM; Benedito, RB; Pereira, WB; de Arruda Torres, P; Ramos, CAF; Costa, JP; da Rocha Tomé, A; de Sousa, DP; de Freitas, RM; de Fatima Formiga Melo Diniz, M and de Almeida, RN (2013). Farnesol: antinociceptive effect and histopathological analysis of the striatum and hippocampus of mice. Fundam. Clinic. Pharmacol., 27: 419-426.
7
De Sousa, DP (2011). Analgesic-like activity of essential oils constituents. Molecules. 16: 2233-2252.
8
Dubey, VK; Ansari, F; Vohora, D and Khanam, R (2015). Possible involvement of corticosterone and serotonin in antidepressant and antianxiety effects of chromium picolinate in chronic unpredictable mild stress induced depression and anxiety in rats. J. Trace. Elem. Med. Biol., 29: 222-226.
9
Duncan, R and Archer, M (2008). Farnesol decreases serum triglycerides in rats: identification of mechanisms including up-regulation of PPARα and down-regulation of fatty acid synthase in hepatocytes. Lipids. 43: 619-627.
10
Flandreau, EI; Ressler, KJ; Owens, MJ and Nemeroff, CB (2012). Chronic overexpression of corticotropin-releasing factor from the central amygdala produces HPA axis hyperactivity and behavioral anxiety associated with gene-expression changes in the hippocampus and paraventricular nucleus of the hypothalamus. Psychoneuroendocrinology. 37: 27-38.
11
Gomes, PB; Feitosa, ML; Silva, MIG; Noronha, EC; Moura, BA; Venâncio, ET; Rios, ERV; de Sousa, DP; de Vasconcelos, SMM; de França Fonteles, MM and de Sousa, FCF (2010). Anxiolytic-like effect of the mono-terpene 1,4-cineole in mice. Pharmacol. Biochem. Behav., 96: 287-293.
12
Guimarães, AG; Serafini, MR and Quintans-Júnior, LJ (2014). Terpenes and derivatives as a new perspective for pain treatment: a patent review. Expert. Opin. Ther. Pat., 24: 243-265.
13
Joo, JH and Jetten, AM (2010). Molecular mechanisms involved in farnesol-induced apoptosis. Cancer Lett., 287: 123-135.
14
Khan, R and Sultana, S (2011). Farnesol attenuates 1,2-dimethylhydrazine induced oxidative stress, inflammation and apoptotic responses in the colon of Wistar rats. Chem. Biol. Interact., 192: 193-200.
15
Lee, YL; Wu, Y; Tsang, HWH; Leung, AY and Cheung, WM (2011). A systematic review on the anxiolytic effects of aromatherapy in people with anxiety symptoms. J. Altern. Complement. Med., 17: 101-108.
16
Li, YJ; Xuan, HZ; Shou, QY; Zhan, ZG; Lu, X and Hu, FL (2012). Therapeutic effects of propolis essential oil on anxiety of restraint-stressed mice. Hum. Exp. Toxicol., 31: 157-165.
17
Linck, VM; da Silva, AL; Figueiró, M; Caramão, EB; Moreno, PRH and Elisabetsky, E (2010). Effects of inhaled Linalool in anxiety, social interaction and aggressive behavior in mice. Phytomedicine. 17: 679-683.
18
Marcuzzi, A; Tommasini, A; Crovella, S and Pontillo, A (2010). Natural isoprenoids inhibit LPS-induced-production of cytokines and nitric oxide in aminobisphos-phonate-treated monocytes. Int. Immunopharmacol., 10: 639-642.
19
Melo, MS; Sena, LCS; Barreto, FJN; Bonjardim, LR; Almeida, JRGS; Lima, JT; De Sousa, DP and Quintans-Júnior, LJ (2010). Antinociceptive effect of citronellal in mice. Pharm. Biol., 48: 411-416.
20
Melo, FHC; Venâncio, ET; De Sousa, DP; De França Fonteles, MM; De Vasconcelos, SMM; Viana, GSB and De Sousa, FCF (2010). Anxiolytic-like effect of Carvacrol (5-isopropyl-2-methylphenol) in mice: involvement with GABAergic transmission. Fundam. Clin. Pharmacol., 24: 437-443.
21
Mizushige, T; Kanegawa, N; Yamada, A; Ota, A; Kanamoto, R and Ohinata, K (2013). Aromatic amino acid-leucine dipeptides exhibit anxiolytic-like activity in young mice. Neurosci. Lett., 543: 126-129.
22
Moreira, MRC; Salvadori, MGDSS; de Almeida, AA; de Sousa, DP; Jordán, J; Satyal, P; de Freitas, RM and de Almeida, RN (2014). Anxiolytic-like effects and mecha-nism of (−)-myrtenol: a monoterpene alcohol. Neurosci. Lett., 579: 119-124.
23
Nic Dhonnchadha, BÁN; Bourin, M and Hascoët, M (2003). Anxiolytic-like effects of 5-HT2 ligands on three mouse models of anxiety. Behav. Brain Res., 140: 203-214.
24
Nóbrega de Almeida, R; Agra, MDF; Negromonte Souto Maior, F and De Sousa, DP (2011). Essential oils and their constituents: anticonvulsant activity. Molecules. 16: 2726-2742.
25
Nogueira Neto, J; de Almeida, A; da Silva Oliveira, J; dos Santos, P; de Sousa, DP and de Freitas, RM (2013). Antioxidant effects of nerolidol in mice hippocampus after open field test. Neuroche. Res., 38: 1861-1870.
26
Petit-Demouliere, B; Chenu, F and Bourin, M (2005). Forced swimming test in mice: a review of antidepressant activity. Psychopharmacology. 177: 245-255.
27
Pinheiro, MMG; Bessa, SO; Fingolo, CE; Kuster, RM; Matheus, ME; Lima, JT; De Sousa, DP and Quintans-Júnior, LJ (2010). Antinociceptive activity of fractions from Couroupita guianensis Aubl. leaves. J. Ethnopharma-col., 127: 407-413.
28
Pires, LF; Costa, LM; Silva, OA; de Almeida, AAC; Cerqueira, GS; de Sousa, DP and de Freitas, RM (2013). Anxiolytic-like effects of carvacryl acetate, a derivative of carvacrol, in mice. Pharm. Biochem. Behav., 112: 42-48.
29
Purnell, JQ; Brandon, DD; Isabelle, LM; Loriaux, DL and Samuels, MH (2004). Association of 24-hour cortisol production rates, cortisol-binding globulin, and plasma-free cortisol levels with body composition, leptin levels, and aging in adult men and women. J. Clin. Endocrinol. Metab., 89: 281-287.
30
Saha, A; Bose, S and Banerjee, S (2013). Anti-anxiety activity of Amorphophallus paeoniifolius tuber in mice. J. Pharm. Res., 6: 748-752.
31
Saiyudthong, S and Marsden, CA (2011). Acute effects of bergamot oil on anxiety-related behaviour and corticos-terone level in rats. Phytother. Res., 25: 858-862.
32
Santhanasabapathy, R and Sudhandiran, G (2015). Farnesol attenuates lipopolysaccharide-induced neurodegeneration in Swiss Albino mice by regulating intrinsic apoptotic cascade. Brain Res., 1620: 42-56.
33
Silva, MIG; de Aquino Neto, MR; Teixeira Neto, PF; Moura, BA; do Amaral, JF; de Sousa, DP; Vasconcelos, SMM and de Sousa, FCF (2007). Central nervous system activity of acute administration of isopulegol in mice. Pharmacol. Biochem. Behav., 88: 141-147.
34
Sulaiman, MR; Perimal, EK; Zakaria, ZA; Mokhtar, F; Akhtar, MN; Lajis, NH and Israf, DA (2009). Pre-liminary analysis of the antinociceptive activity of zerumbone. Fitoterapia. 80: 230-232.
35
Tatman, D and Mo, H (2002). Volatile isoprenoid constituents of fruits, vegetables and herbs cumulatively suppress the proliferation of Murine B16 melanoma and human HL-60 leukemia cells. Cancer Lett., 175: 129-139.
36
ORIGINAL_ARTICLE
Effect of doxycycline and Lactobacillus probiotics on mRNA expression of ABCC2 in small intestines of chickens
Probiotics and antibiotics are widely used in poultry and may alter drug bioavailability by affecting the expression of intestinal ATP-binding cassette (ABC) efflux transporters. Therefore the aim of the present investigation was to evaluate the effect of Lactobacilli probiotics, administered alone or in combination with doxycycline, on the expression of ABCB1 (gene, encoding P-glycoprotein), ABCC2 (gene, encoding multidrug resistance protein 2, MRP2) and ABCG2 (gene, encoding breast cancer resistance protein) mRNAs in chicken using RT-PCR. Duc one-day-old chicks (n=24) were divided equally in four groups: untreated control, probiotics supplemented group, probiotics plus doxycycline treated chickens and antibiotic administered group. Expression of ABCC2 mRNA was affected by doxycycline or by combination of Lactobacillus plantarum, L. brevis and L. bulgaricus and the antibiotic in the intestines. These results can be used as a basis for further functional studies to prove the beneficial effect on limitation of the absorption of toxins and improvement of efflux of endogenous substances and xenobiotics when the combination of doxycycline and Lactobacillus spp. probiotics are administered to poultry.
https://ijvr.shirazu.ac.ir/article_3913_c7f93e07133a0a6f712a3bbbc9766c4c.pdf
2016-12-01
265
267
10.22099/ijvr.2016.3913
ABC transporters
Doxycycline
Lactobacillus probiotics
Poultry
A.
Milanova
akmilanova@gmail.com
1
Department of Pharmacology, Physiology of Animals and Physiological Chemistry, Faculty of Veterinary Medicine, Trakia University, 6000 Stara Zagora, Bulgaria
LEAD_AUTHOR
I.
Pavlova
2
Department of Pharmacology, Physiology of Animals and Physiological Chemistry, Faculty of Veterinary Medicine, Trakia University, 6000 Stara Zagora, Bulgaria
AUTHOR
V.
Yordanova
3
BSc in Molecular Biology, Molecular Diagnostics Unit, Hospital for Active Treatment “Dr. Atanas Dafovski”, Kurdjali, Bulgaria
AUTHOR
S.
Danova
4
The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences (BAS), 26, Akad. G. Bontchev, Sofia, Bulgaria
AUTHOR
Angelakis, E; Bastelica, D; Ben Amara, A; El Filali, A; Dutour, A; Mege, JL; Alessi, MC and Raoult, D (2012). An evaluation of the effects of Lactobacillus ingluviei on body weight, the intestinal microbiome and metabolism in mice. Microb. Pathog., 52: 61-68.
1
Borst, P; Zelcer, N and van de Wetering, K (2006). MRP2 and 3 in health and disease. Cancer Lett., 8: 51-61.
2
Guo, M; Sun, Y; Zhang, Y; Bughio, S; Dai, X; Ren, W and Wang, L (2014). E. coli infection modulates the pharma-cokinetics of oral enrofloxacin by targeting P-glycoprotein in small intestine and CYP450 3A in liver and kidney of broilers. PLoS One. 9: e87781.
3
Haritova, AM (2006). PK-PD modeling of fluoroquinolones and ABC transporters in poultry. Ph.D. Thesis, University Utrecht, The Netherlands. PP: 111-144.
4
Kabir, SM (2009). The role of probiotics in the poultry industry. Int. J. Mol. Sci., 10: 3531-3546.
5
Pan, D and Yu, Z (2014). Intestinal microbiome of poultry and its interaction with host and diet. Gut Microbes. 5: 108-119.
6
Pavlova, I (2015). Effect of probiotics on doxycycline disposition in gastro-intestinal tract of poultry. Bulg. J. Vet. Med., 18: 248-257.
7
Schrickx, JA and Fink-Gremmels, J (2008). Implications of ABC transporters on the disposition of typical veterinary medicinal products. Eur. J. Pharmacol., 13: 510-519.
8
Stojančević, M; Bojić, G; Salami, HA and Mikov, M (2014). The influence of intestinal tract and probiotics on the fate of orally administered drugs. Curr. Issues Mol. Biol., 16: 55-68.
9
Su, L; Dong, L; Bughio, S; Guo, M and Wang, L (2014). Effect of colibacillosis or coccidiosis on expression of breast cancer resistance protein in small intestine and liver of chickens. J. Vet. Pharmacol. Ther., 37: 53-58.
10
Tropcheva, R; Hristova, J; Georgieva, R; Salageanu, A; Sgouras, DN and Danova, S (2013). In vitro assessment of prebiotic utilization by dairy lactobacilli. Bulg. J. Agricult. Sci., 19: 105-107.
11
Vandesompele, J; Kubista, M and Pfaffl, MW (2009). Reference gene validation software for improved normali-zation. In: Logan, J; Edwards, K and Saunders, N (Eds.), Real-time PCR: current technology and applications. (1st Edn.), London, Caister Academic Press. PP: 47-64.
12
Wang, Y; Liu, Y; Sidhu, A; Ma, Z; McClain, C and Feng, W (2012). Lactobacillus rhamnosus GG culture super-natant ameliorates acute alcohol-induced intestinal permeability and liver injury. Am. J. Physiol. Gastrointest. Liver Physiol., 303: G32-41.
13
Yin, J; Zhang, XX; Wu, B and Xian, Q (2015). Metagenomic insights into tetracycline effects on microbial community and antibiotic resistance of mouse gut. Ecotoxicology. 24: 2125-2132.
14
ORIGINAL_ARTICLE
Effects of dietary supplementation of mannan-oligosaccharide on virus shedding in avian influenza (H9N2) challenged broilers
Avian influenza (AI) is a highly contagious disease causing significant economic losses worldwide. The aim of this study is to evaluate the effect of mannan-oligosaccharide (MOS) on tracheal and cloacal virus shedding in AI challenged broilers and contamination of environment with H9N2. A total of 300 1-day-old-broiler chicks were randomly divided into 3 groups (A, B and C) and supplemented 0.2, 0.5 and 0.0% MOS, respectively in NRC recommended diet for 36 days. On day 21 the groups were further split into two sub groups A+ve, A-ve, B+ve, B-ve, C+ve and C-ve with 5 replicates each. The positive groups were shifted to remote sheds and were challenged intranasally with 0.1 ml of reference virus (AIV; Pk-UDL/01/08 H9N2) with EID50 = 10-6.66. Treatment reduces (P<0.05) cloacal virus shedding from day 24 to 26 and 28 to 32. Tracheal virus shedding was lower (P<0.05) on days 25-26 and 28-30 in treatment groups. Day 27 showed highest (P>0.05) virus shedding in all groups. However the reduction of viral shedding is faster in treatment groups and showed no virus shedding on day 32. Maternal antibody titer against AI showed a declining pattern but MOS influenced (P<0.05) the titer in treated groups. Hence the use of MOS may constitute a novel and effective plausible alternative that reduces the spread of disease by decreasing virus shedding and contamination of environment from AIV (H9N2) infection in poultry.
https://ijvr.shirazu.ac.ir/article_3914_ad1cf6004acd4f853dfd4bae54a9d457.pdf
2016-12-01
268
272
10.22099/ijvr.2016.3914
Avian influenza
Broiler
MOS
SAF-Mannan
T.
Akhtar
1
MPhil/Ms in Physiology, Department of Physiology, University of Veterinary and Animal Sciences, Lahore-5400, Punjab, Pakistan
AUTHOR
G.
Ara
2
MPhil/Ms in Genetic Engineering and Biotechnology, Institute of Biotechnology and Genetic Engineering, The University of Agriculture, Peshawar-25120, KPK, Pakistan
AUTHOR
N.
Ali
3
MPhil/Ms in Livestock Management, Department of Livestock Management, Faculty of Animal Husbandry and Veterinary Sciences, The University of Agriculture, Peshawar-25120, KPK, Pakistan
AUTHOR
F.
ud Din Mufti
4
MPhil/Ms in Biotechnology, Department of Biotechnology, Quaid-i-Azam University, Islamabad, Pakistan
AUTHOR
M.
Imran Khan
imran.khan@aup.edu.pk
5
Department of Animal Health, Faculty of Animal Husbandry and Veterinary Sciences, The University of Agriculture, Peshawar-25120, KPK, Pakistan
LEAD_AUTHOR
Alexander, DJ and Chettle, NJ (1977). Procedures for the haemagglutination and the haemagglutination inhibition tests for avian infectious bronchitis virus. Avian Pathol., 6: 9-17.
1
Bhatti, BM (1995). A review of incidence of avian influenza disease. Agro-Livestock News. 1: 1-4.
2
Cotter, PF; Malzone, A; Paluch, B; Lilburn, MS and Sefton, AE (2000). Modulation of humoral immunity in commercial laying hens by a dietary prebiotic. Poult. Sci., 79(S1): 38 (abst.).
3
Duncan, DB (1955). Multiple range and multiple F tests. Biometrics. 11: 1-42.
4
Fang, SB; Lee, HC; Hu, JJ; Hou, SY; Liu, HL and Fang, HW (2009). Dose-dependent effect of Lactobacillus rhamnosus on quantitative reduction of faecal rotavirus shedding in children. J. Trop. Pediatr., 55: 297-301.
5
Ganguli, K; Meng, D; Rautava, S; Lu, L; Walker, WA and Nanthakumar, N (2013). Probiotics prevent necrotizing enterocolitis by modulating enterocyte genes that regulate innate immune-mediated inflammation. Am. J. Physiol. Gastrointest. Liver Physiol., 304: 132-141.
6
Glenn, GR and Roberfroid, MB (1995). Dietary modulation of the human colonic microbiota: introducing the concepts of prebiotics. J. Nutr., 125: 1401-1412.
7
Havenaar, R and Huis In’t Veld, JHJ (1992). Probiotics: A general view in the lactic acid bacteria in health and disease. Vol. 1. WOOD JB.
8
Janardhana, V; Broadway, MM; Bruce, MP; Lowenthal, JW; Geier, MS; Hughes, RJ and Bean, AG (2009). Prebiotics modulate immune responses in the gut-associated lymphoid tissue of chickens. J. Nutr., 139: 1404-1409.
9
Kawase, M; He, F; Kubota, A; Harata, G and Hiramatsu, M (2010). Oral administration of lactobacilli from human intestinal tract protects mice against influenza virus infection. Lett. Appl. Microbiol., 51: 6-10.
10
Lourenço, MC; de Souza, AM; Hayashi, RM; da Silva, AB and Santin, E (2016). Immune response of broiler chickens supplemented with prebiotic from Sacharomyces cerevisiae challenged with Salmonella enteritidis or Minnesota. J. Appl. Poult. Res., 25: 165-172.
11
National Research Council (2001). Nutrient requirements for poultry. 11th Revised ED. Washington, D.C., USA, NationalAcademy Press.
12
Noble, GR (1982). Epidemiological and clinical aspects of influenza. In: Beare, AS (Ed.), Basic and applied influenza research. Boca Raton (Florida), CRC Press. PP: 11-50.
13
Oliveira, MC; Figueiredo-Lima, DF; Faria, DE; Marques, RH and Moraes, VMB (2009). Effect of mannanoligo-saccharides and/or enzymes on antibody titers against infectious bursal and Newcastle disease virus. Arq. Bras. Med. Vet. Zootec., 61: 6-11.
14
Oyofo, BA; DeLoach, JR; Corrier, DE; Norman, JO; Ziprin, RL and Mollenhauer, HH (1989). Prevention of Salmonella typhimurium colonization of broilers with D-mannose. Poult. Sci., 68: 1357-1360.
15
Poorbaghi, SL; Dadras, H; Gheisari, HR; Mosleh, N; Firouzi, S and Roohallazadeh, H (2013). Effects of Lactobacillus acidophilus and inulin on faecal viral shedding and immunization against H9N2 avian influenza virus. J. Appl. Microbiol., 116: 667-676.
16
Qaisrani, SN; van Krimpen, MM; Kwakkel, RP; Verstegen, MWA and Hendriks, WH (2015). Diet structure, butyric acid, and fermentable carbohydrates influence growth performance, gut morphology, and cecal fermentation characteristics in broilers. Poult. Sci., 94: 2152-2164.
17
Qiao, H; Duffy, LC; Griffiths, E; Dryja, D; Leavens, A; Rossman, JON; Rich, G; Riepenhoff-Talty, M and Locniskar, M (2002). Immune responses in rhesus rotavirus-challenged Balb/c mice treated with Bifido-bacteria and Prebiotic supplements. Pediatr. Res., 51: 750-755.
18
Raju, MVLN and Devegowda, G (2002). Esterified-glucomannan in broiler chicken diets-contaminated with aflatoxin, ochratoxin and T-2 toxin: Evaluation of its binding ability (in vitro) and efficacy as immunomodulator. Asian Aust. J. Anim. Sci., 15: 1051-1056.
19
Reed, LJ and Muench, H (1938). A simple method of estimating fifty percent endpoints. Am. J. Epidemiol., 27: 493-497.
20
Ribeiro, AML; Vogt, LK; Canal, CW; Cardoso, M; Labres, RV; Sreack, AF and Bessa, MC (2007). Effects of prebiotics and probiotics on the colonization and immune response of broiler chickens challenged with Salmonella Enteritidis. Braz. J. Poult. Sci., 9: 193-200.
21
Rickard, ER; Thigpen, M and Crowley, JH (1944). The isolation of influenza A virus by the intra-allantoic inoculation of chick embryos with untreated throat-washings. J. Immunol., 49: 263-271.
22
Saf-Agric Inc. (2007). SAF-Mannan Product Information Sheet. www.saf-agri.com/english/inomos.htm., Accessed Aug.17.2015.
23
Schley, PD and Field, CJ (2002). The immune-enhancing effects of dietary fibres and prebiotics. Brit. J. Nutr., 87(S2): 221-230.
24
Shahir, MH; Sharifi, M; Afsarian, O and Mousavi, SS (2014). A comparison of the effects of commercial prebiotic (safmannan®, biomos® and fermacto®) on performance, egg quality and antibody titer of avian influenza and Newcastle disease in laying hens. J. Vet. Res., 69: 79-84.
25
Shashidhara, RG and Devegowda, G (2003). Effect of dietary mannan oligosaccharide on broiler breeder production traits and immunity. Poult. Sci., 82: 1319-1325.
26
Silva, VK; da Silva, JDT; Torres, KAA; de Faria Filho, DE; Hada, FH and de Moraes, VB (2009). Humoral immune response of broilers fed diets containing yeast extract and prebiotics in the prestarter phase and raised at different temperatures. J. Appl. Poult. Res., 18: 530-540.
27
Spring, P; Wenk, C; Dawson, KA and Newman, KE (2000). The effect of dietary mannaoligosaccharides on cecal parameters and the concentrations of enteric bacteria in the ceca of salmonella-challenged broiler chicks. Poult. Sci., 79: 205-211.
28
Steel, RGD and Dickey, DAJH (1997). Principles and procedures of statistics: a biometrical approach. McGraw-Hill series in probability and statistics.
29
Strickling, JA; Harmon, DL; Dawson, KA and Gross, KL (2000). Evaluation of oligosaccharide addition to dog diets: influences on nutrient digestion and microbial populations. Anim. Feed Sci. Technol., 86: 205-219.
30
Tohid, T; Hasan, G and Alireza T (2010). Efficacy of mannanoligosaccharides and humate on immune response to Avian Influenza (H9) disease vaccination in broiler chickens. Vet. Res. Commun., 34: 709-717.
31
Yang, Y; Iji, PA; Kocher, A; Mikkelsen, LL and Choct, M (2007). Effects of mannanoligosaccharide on growth performance, the development of gut microflora, and gut function of broiler chickens raised on new litter. J. Appl. Poult. Res., 16: 280-288.
32
Youn, LH; Lee, YN; Lee, DH; Park, JK; Yuk, SS; Lee, HJ; Yeo, JM; Yang, SY; Lee, JB; Park, SY; Choi, IS and Song, CS (2012). Effect of intranasal administration of Lactobacillus fermentum CJL-112 on horizontal trans-mission of influenza virus in chickens. Poult. Sci., 91: 2517-2522.
33
ORIGINAL_ARTICLE
Isolation of Clostridium difficile and molecular detection of binary and A/B toxins in faeces of dogs
The aim of this study was to isolate Clostridium difficile from dogs’ faeces, and to study the frequency of its virulence genes. A total of 151 samples of dogs’ faeces were collected. The isolation of C. difficile was performed by using the bacterial culture methods followed by DNA extraction using boiling method. Multiplex PCR method was performed for identification of tcdA, tcdB, cdtA and cdtB genes and single method was carried out for detection of tcdC. Twelvesamples (7.9%) were positive in bacteriological assay and based on molecular assay, 66.7% of the isolates (8 of 12 C. difficile isolated) had shown tcdA+, tcdB+ profile. This is the first investigation on molecular assay of C. difficilein Iran’s dog population.
https://ijvr.shirazu.ac.ir/article_3916_5bc930789650a00668f44213f35c16b5.pdf
2016-12-01
273
276
10.22099/ijvr.2016.3916
Clostridium difficile
dog
Molecular detection
M.
Ghavidel
1
Ph.D. Student in Bacteriology, Department of Microbiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
AUTHOR
H.
Salari Sedigh
hssedigh@um.ac.ir
2
Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
LEAD_AUTHOR
J.
Razmyar
3
Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
AUTHOR
Aldous, WK; Pounder, JI; Cloud, JL and Woods, GL (2005). Comparison of six methods of extracting Mycobacterium tuberculosis DNA from processed sputum for testing by quantitative real-time PCR. J. Clin. Microbiol., 43: 2471-2473.
1
Antikainen, J; Pasanen, T; Mero, S; Tarkka, E; Kirveskari, J; Kotila, S; Mentula, S; Könönen, E; Virolainen‐ Julkunen, AR and Vaara, M (2009). Detection of virulence genes of Clostridium difficile by multiplex PCR. APMIS., 117: 607-613.
2
Clooten, J; Kruth, S; Arroyo, L and Weese, JS (2008). Prevalence and risk factors for Clostridium difficile colonization in dogs and cats hospitalized in an intensive care unit. Vet. Microbiol., 129: 209-214.
3
Dabard, J; Dubos, F; Martinet, L and Ducluzeau, R (1979). Experimental reproduction of neonatal diarrhea in young gnotobiotic hares simultaneously associated with Clostridium difficile and other Clostridium strains. Infect. Immun., 24: 7-11.
4
Deneve, C; Janoir, C; Poilane, I; Fantinato, C and Collignon, A (2009). New trends in Clostridium difficile virulence and pathogenesis. Int. J. Antimicrob. Agents. 33: S24-S28.
5
Doosti, A and Mokhtari-Farsani, A (2014). Study of the frequency of Clostridium difficile tcdA, tcdB, cdtA and cdtB genes in feces of calves in south west of Iran. Ann. Clin. Microbiol. Antimicrob., 13: 1-6.
6
Fedorko, DP and Williams, EC (1997). Use of cycloserine-cefoxitinfructose agar and L-proline-aminopeptidase (PRO Discs) in the rapid identification of Clostridium difficile. J. Clinic. Microbiol., 35: 1258-1259.
7
Fooladi, AAI; Rahmati, S; Abadi, JFM; Halabian, R; Sedighian, H; Soltanpour, MJ and Rahimi, M (2014). Isolation of Clostridium difficile and detection of A and B toxins encoding genes. Int. J. Entric. Pathog., 2: e15238.
8
Frazier, KS; Herron, AJ; Hines, ME; Gaskin, JM and Altman, NH (1993). Diagnosis of enteritis and entero-toxemia due to Clostridium difficile in captive ostriches (Struthio camelus). J. Vet. Diagn. Invest., 5: 623-625.
9
Ghose, C (2013). Clostridium difficile infection in the twenty-first century. Emerg. Microbes. Infect., 2: 1-8.
10
Hasanzade, A and Rahimi, E (2013). Isolation of Clostridium difficile from turkey and ostrich meat sold in meat stores of Isfahan city. I.J.A.B.B.R., 1: 963-967.
11
Jalali, M; Khorvash, F; Warriner, K and Weese, JS (2012). Clostridium difficile infection in an Iranian hospital. BMC. Res. Notes. 5: 159.
12
Kevin, K; Brazier, JS; Post, KW; Weese, S and Songer, JG (2007). Prevalence of PCR ribotypes among Clostridium difficile isolates from pigs, calves, and other species. J. Clin. Microbiol., 45: 1963-1964.
13
Koene, MGJ; Mevius, D; Wagenaar, JA; Harmanus, C; Hensgens, MPM; Meetsma, AM; Putirulan, FF; Bergen, MAP and Kuijper, EJ (2011). Clostridium difficile in Dutch animals: their presence, characteristics and similarities with human isolates. Clin. Microbiol. Infect., 18: 778-784.
14
Marks, SL and Kather, EJ (2003). Antimicrobial sus-ceptibilities of canine Clostridium difficile and Clostridium perfringens isolates to commonly utilized antimicrobial drugs. Vet. Microbiol., 94: 39-45.
15
Marks, S; Kather, EJ; Kass, PH and Melli, AC (2002). Genotypic and phenotypic characterization of Clostridium perfringens and Clostridium difficile in diarrheic and healthy dogs. J. Vet. Intern. Med., 16: 533-540.
16
Marks, S; Rankin, S; Byrne, B and Weese, J (2011). Enteropathogenic bacteria in dogs and cats: diagnosis, epidemiology, treatment, and control. J. Vet. Intern. Med., 25: 1195-1208.
17
McKee, R; Mangalea, M; Purcell, E; Borchardt, E and Tamayo, R (2013). The second messenger cyclic Di-GMP regulates Clostridium difficile toxin production by controlling expression of sigD. J. Bacteriol., 195: 5174-5185.
18
O’Neill, G; Adams, JE; Bowman, RA and Riley, TV (1993). A molecular characterization of Clostridium difficile isolates from humans, animals and their environments. Epidemiol. Infect., 111: 257-264.
19
Ossiprandi, MC; Buttrini, M; Bottarelli, E and Zerbini, L (2012). A preliminary molecular typing by PCR assays of Clostridium perfringens and Clostridium difficile isolates from dogs. AiM., 2: 87-92.
20
Persson, S; Torpdahl, M and Olsen, KEP (2008). New multiplex PCR method for the detection of Clostridium difficile toxin A (tcdA) and toxin B (tcdB) and the binary toxin (cdtA⁄cdtB) genes applied to a Danish strain collection. Clin. Microbiol. Infect., 14: 1057-1064.
21
Pituch, H; Obuch‐Woszczatyñski, P; Van Den Braak, N; Van Belkum, A; Kujawa, M; Luczak, M and Meisel‐ Mikolajczyk, F (2002). Variable flagella expression among clonal toxin A–/B+ Clostridium difficile strains with highly homogeneous flagellin genes. Clin. Microbiol. Infect., 8: 187-188.
22
Rahimi, E; Jalali, M and Weese, JS (2014). Prevalence of Clostridium difficile in raw beef, cow, sheep, goat, camel and buffalo meat in Iran. BMC. Public Health. 14: 119.
23
Riley, T; Adams, J; O’Neill, G and Bowman, R (1991). Gastrointestinal carriage of Clostridium difficile in cats and dogs attending veterinary clinics. Epidemiol. Infect., 107: 659-665.
24
Silva, ROS; Santos, RLR; Pires, PS; Pereira, LC; Duarte, MC; de Assis, RA and Lobato, FC (2013). Detection of toxins A/B and isolation of Clostridium difficile and Clostridium perfringens from dogs in Minas Gerais, Brazil. Braz. J. Microbiol., 44: 133-137.
25
Weese, JS; Staempfli, HR; Prescott, JF; Kruth, SA; Greenwood, SJ and Weese, HE (2001). The roles of Clostridium difficile and enterotoxigenic Clostridium perfringens in diarrhea in dogs. J. Vet. Intern. Med., 15: 374-378.
26
Wetterwik, K; Trowald-Wigh, G; Fernström, L and Krovacek, K (2013). Clostridium difficile in faeces from healthy dogs and dogs with diarrhea. Acta Vet. Scand., 55: 23.
27
ORIGINAL_ARTICLE
The effects of sub-chronic administration of sub-lethal doses of amitraz/xylene on selected reproductive parameters of male Wistar rats
This study investigated the effects of sub-chronic administration of sub-lethal doses of amitraz on some testicular parameters of Albino rats. Twenty-four adult male Albino rats (100 ± 10 g) randomly assigned into four groups were used for the study. Groups A, B and C received 10.0, 2.0 and 0.4 mg/kg amitraz in 10 ml/kg water while group D received equivalent volume of water orally and daily for 84 days. Serum testosterone levels (TESL) were assessed on days 0, 28, 56 and 84. Epididymal sperm reserve (ESR), testicular sperm reserve (TSR), testicular weight index (TWI) and testicular histology were evaluated at the end of the experiment. Results revealed dose-dependent reduction (P<0.05) in the mean TESL, ESR and TSR in the amitraz-treated groups as the dose of the amitraz increased. Histological study revealed testicular degeneration characterized by depopulation of seminiferous tubules and depletion of the spermatogenic cells in rats in group A. It was concluded that sub-chronic administration of sub-lethal doses of amitraz could lead to reduced sperm quantity.
https://ijvr.shirazu.ac.ir/article_3917_15004cbae8bbecaba6384ba6d2e8b15d.pdf
2016-12-01
277
280
10.22099/ijvr.2016.3917
Amitraz
Rat
Sperm
Testis
Testosterone
V. U.
Omoja
valentine.omoja@unn.edu.ng
1
Department of Veterinary Physiology and Pharmacology, Faculty of Veterinary Medicine, University of Nigeria, Nsukka Enugu State, Nigeria
LEAD_AUTHOR
S. M.
Anika
2
Department of Veterinary Physiology and Pharmacology, Faculty of Veterinary Medicine, University of Nigeria, Nsukka Enugu State, Nigeria
AUTHOR
I. U.
Asuzu
3
Office of the Vice-Chancellor, Federal University Oye-Ekiti, Ekiti State, Nigeria
AUTHOR
Bancroft, JD and Stevens, A (1977). Theory and practice of histological techniques. 1st Edn., Edinburgh, Churchill Livingstone. PP: 16-64.
1
Bukowska, B (2003). Effects of 2,4-D and its metabolite 2,4-dichlorophenol on antioxidant enzymes and level of glutathione in human erythrocytes. Comp. Biochem. Physiol. C Comp. Pharmacol., 135: 435-441.
2
Chatfield, C (1983). Statistics for technology: a course in applied statistics. 3rd Edn., London, Chapman and Hull. P: 140.
3
Crofton, KM; Boncek, VM and Reiter, LW (1989). Acute effects of amitraz on the acoustic startle response and motor activity. Pestic. Sci., 27: 1-11.
4
Gwaltney-Brant, S (2004). Insecticides and molluscicides. Clinical veterinary toxicology. (1st Edn.), USA, Konnie Plumlee, Mosby Inc., PP: 177-178.
5
Hsu, WH and Schaffer, DD (1988). Effects of topical application of amitraz on plasma glucose and insulin concentrations in dogs. Am. J. Vet. Res., 49: 130-131.
6
Jonsson, NN; Miller, RJ; Kempc, DH; Knowles, A; Ardila, AE; Verrall, RG and Rothwell, JT (2010). Rotation of treatments between spinosad and amitraz for the control of Rhipicephalus (Boophilus) microplus populations with amitraz resistance. Vet. Parasitol., 169: 157-164.
7
Kruk, I and Bounias, M (1992). Chemiluminescence from oxidation of formamidine amitraz. The generation of cytotoxic oxygen species and electronically excited com-pounds. Sci. Total Environ., 123-124: 195-203.
8
Mills, T; Stopper, V and Wiedmeier, V (2004). Effects of castration and androgen replacement on the hemodynamics of penile erection in the rat. Biol. Reprod., 54: 234-238.
9
Nicolas, B; Andrew, YL; Robert, JM; Huguette, G; Jean-Michel, D; Ronald, BD and John, EG (2008). In vitro and in vivo evaluation of deltamethrin and amitraz mixtures for the control of Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) in New Caledonia. Vet. Parasitol., 155: 110-119.
10
Oishi, S (2002). Effects of propylparaben on the male re-productive system. Food Chem. Toxicol., 40: 1807-1813.
11
Omoja, VU (2015). Amitraz toxicity, effect of vitamin C in amitraz-induced poisoning in rats and tissue levels of amitraz in exposed West African Dwarf sheep. Ph.D. Thesis, University of Nigeria, Nsukka. P: 61.
12
Omoja, VU; Asuzu, IU and Anika, SM (2015). Assessment of the hepatic and renal effects of sub-chronic administra-tion of sub-lethal doses of amitraz/xylene in Albino Wister rats. Comp. Clin. Pathol., 25: 203-209.
13
Shabsigh, R (2005). Testosterone therapy in erectile dysfunc-tion and hypogonadism. J. Sex. Med., 2: 785-792.
14
Soberans, NC; Santamaria, MV; Fragoso, HS and Garcia, ZV (2002). First case reported of amitraz resistance in the cattle tick Boophilus microplus in Mexico. Tec. Pecu. Mex., 40: 81-92.
15
Ueng, TH; Hung, CC; Wang, HW and Chan, PK (2004). Effects of amitraz on cytochrome P450-dependent mono-oxygenases and estrogenic activity in MCF-7 human breast cancer cells and immature female rats. Food Chem. Toxicol., 42: 1785-1794.
16
ORIGINAL_ARTICLE
Molecular identification and successful treatment of Chlamydophila psittaci (genotype B) in a clinically affected Congo African grey parrot (Psittacus erithacus erithacus)
Avian chlamydiosis is caused by Chlamydiophila psittaci with the highest infection rate in parrots (Psittacidae) and pigeons (Columbiformes). A two-year-old Congo African grey parrot was examined since the bird had shown clinical signs of anorexia, depression, diarrhea, and mild dyspnea and based on biochemical and hemathological analysis the bird was diagnosed as having anemia, leukocytosis, heterophilia, lymphopenia and monocytosis. With regards to clinical and paraclinical findings, the case was diagnosed to be carrying Chlamydiophila spp. In addition, choanal cleft and cloaca swabs were positive for Chlamydiophila spp. in a diagnostic polymerase chain reaction (PCR) (600 bp amplicon). Polymerase chain reaction products were typed by ompA gene-based PCR, using CTU/CTL primers (1050 bp amplicon). The PCR product sequence was compared with the sequences obtained from GenBank. The phylogenetic tree has revealed 100% identity with genotype B obtained from previous studies. The bird was hospitalized and treated with doxycycline regimen for 45 days, with a weekly sampling process to trace the presence of C. psittaci DNA in faecal and choanal swabs, this process continued to the point where the specimens turned negative after two weeks. Laboratory and radiology results were within normal limits after the treatment. Genotype B is predominantly isolated from Columbidae and there have not been any reports regarding the clinically affected African gray parrot with this genotype. Subsequently, to the best of our knowledge, this is the first report of chlamydiosis by genotype B on Congo African grey parrot.
https://ijvr.shirazu.ac.ir/article_3918_389c0fbaa215317f03d8a424a7d4f6cd.pdf
2016-12-01
281
285
10.22099/ijvr.2016.3918
Avian chlamydiosis
Chlamydophila psittaci genotype B
Congo African grey parrot
ompA gene
PCR
J.
Razmyar
jrazmyar@ut.ac.ir
1
Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
LEAD_AUTHOR
M.
Rajabioun
2
Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
AUTHOR
M.
Zaeemi
3
Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
AUTHOR
A.
Afshari
4
Department of Nutrition, Faculty of Medicine, Mashhad University of Medical Science, Mashhad, Iran
AUTHOR
Andersen, AA (1997). Two new serovars of Chlamydia psittaci from North American birds. J. Vet. Diagnost. Inves., 9: 159-164.
1
Andersen, AA and Vanrompay, D (2003). Avian chlamydiosis (Psittacosis, Ornithosis). In: Diseases of poultry. 11th Edn., Ames, Iowa, USA, IowaStateUniversity Press. PP: 863-879.
2
Arraiz, N; Bermudez, V; Urdaneta, B; Mujica, E; Sanchez, MP; Mejía, R; Prieto, C; Escalona, C and Mujica, A (2012). Evidence of zoonotic Chlamydophila psittaci transmission in a population at risk in Zulia state, Venezuela. Rev. Panam. Salud. Publica. (Bogota). 14: 305-314.
3
Bush, RM and Everett, K (2001). Molecular evolution of the chlamydiaceae. Int. J. Syst. Evol. Microbiol., 51: 203-220.
4
Campbell, TW and Ellis, CK (2007). Avian and exotic animal hematology and cytology. 3rd Edn., Ames, Iowa, Blackwell Pub., PP: 326-328.
5
Fudge, AM (2000). Avian liver and gastrointestinal testing. In: Fudge, (Ed.), Laboratory medicine avian and exotic pets. (1st Edn.), USA, Saunders Pub., PP: 47-55.
6
Geens, T; Desplanques, A; Van Loock, M;Bönner, BM; Kaleta, EF; Magnino, S; Andersen, A;Everett, KDA andVanrompay, D (2005). Sequencing of the Chlamydo-phila psittaci ompA gene reveals a new genotype, E/B, and the need for a rapid discriminatory genotyping method. J. Clin. Microbiol., 43: 2456-2461.
7
Harcourt-Brown, N and Chitty, J (2005). BSAVA manual of psittacine birds. 2nd Edn., Wareham, UK, BSAVA Publications. P: 69. Heddema, ER;Van Hannen, EJ; Duim, B;Vandenbroucke-Grauls, CMJE andPannekoek, Y (2006). Genotyping of Chlamydophila psittaci in human samples. Emerg. Infect. Dis., 12: 1989-1990.
8
Kaleta, EF and Taday, EM (2003). Avian hosts range of Chlamydophila spp. based on isolation, antigen detection, and serology. Avian Pathol., 32: 435-462.
9
Madani. SA and Peighambari, SM (2013). PCR-based diagnosis, molecular characterization and detection of atypical strains of avian Chlamydia psittaci in companion and wild birds. Avian Pathol., 42: 38-44.
10
Natt, MP and Herrick, CA (1952). A new blood diluent for counting the erythrocytes and leucocytes of the chicken. Poult. Sci., 31: 735-738.
11
OIE, A (2008). Manual of diagnostic tests and vaccines for terrestrial animals. Office International des Epizooties, Paris, France. PP: 1092-1106.
12
Piasecki, T; Chrząstek, K and Wieliczko, A (2012). Detection and identification of Chlamydophila psittaci in asymptomatic parrots in Poland. BMC. Vet. Res., 8: 233-239.
13
Pickett, MA; Everson, JS and Clarke, IN (1988). Chlamydia psittaci ewe abortion agent: complete nucleotide sequence of the major outer membrane protein gene. FEMS. Microbiol. Lett., 55: 229-234.
14
Read, TD;Joseph, SJ; Didelot, X; Liang, B;Patel, L and Dean, D (2013). Comparative analysis of Chlamydia psittaci genomes reveals the recent emergence of a pathogenic lineage with a broad host range. MBio., 4: e00604-00612.
15
Sachse, K and Hotzel, H (2003). Detection and differentiation of Chlamydiae by nested PCR. In: Sachse, K and Frey, J (Eds.), PCR detection of microbial pathogens. (1st Edn.), New Jersey, USA, Humana Press. PP: 123-136.
16
Sachse, K; Laroucau, K;Hotzel, H;Schubert, E; Ehricht, R and Slickers, P (2008). Genotyping of Chlamydophila psittaci using a new DNA microarray assay based on sequence analysis of ompA genes. BMC. Microbiol., 8: 63-75.
17
Schachter, J (1999). Infection and disease epidemiology. In: Stephens, RS (Ed.), Chlamydia: intracellular biology, pathogenesis, and immunity. Washington, D.C., USA, ASM Press. PP: 139-169.
18
West, A (2011). A brief review of Chlamydophila psittaci in birds and humans. J. Exot. Pet. Med., 20: 18-20.
19
Zhang, YX; Morrison, SJ; Caldwell, HD and Baehr, W (1989). Cloning and sequence analysis of the major outer membrane protein genes of two Chlamydia psittaci strains. Infect. Immun., 57: 1621-1625.
20