%0 Journal Article %T Remediation of zearalenone mycotoxin contamination in rumen fluid by phytochemical compounds of Zataria multiflora %J Iranian Journal of Veterinary Research %I Shiraz University %Z 1728-1997 %A Shah-Vardi, M. %A Nazaryanpour, E. %A Nejad-Ebrahimi, S. %A Farzaneh, M. %D 2022 %\ 12/01/2022 %V 23 %N 4 %P 302-309 %! Remediation of zearalenone mycotoxin contamination in rumen fluid by phytochemical compounds of Zataria multiflora %K Metabolite profiling %K Shirazi thyme %K Triterpenes %K Zearalenone degradation %R 10.22099/ijvr.2022.39561.5746 %X Background: Zearalenone (ZEA), which is one of the most prevalent wheat and corn seeds mycotoxins causes acute and chronic toxicities in ruminants, poultry, and aquatic animals. Among commercial toxin binders, only a few active charcoals have the significant ability to adsorb ZEA contamination; nevertheless, active charcoal is not considered a sound additive by the feed industry. Aims: This study aimed to screen and identify the ZEA-degradation compounds of the Zataria multiflora (Shirazi thyme) in the cattle rumen fluid. Methods: In this investigation, essential oil and different extracts (n-hexane, ethyl acetate, and methanol) of the aerial part of Shirazi thyme (at three concentrations of 0.5, 1, and 2 mg/ml) were screened to reduce ZEA contamination conditions (2 µg/ml) in rumen fluid. ZEA-content was analyzed by high-performance liquid chromatography (HPLC) with a fluorescence detector. In addition, Shirazi thyme phytochemical compounds responsible for eliminating ZEA were localized by HPLC-based activity profiling and then identified by mass spectrometry (LC-MS). Results: Both n-hexane and methanol extracts of Z. multiflora, considerably remediated ZEA (63-78%) from rumen fluid. According to HPLC-based activity profiling of Z. multiflora extract and LC-MS analysis, two triterpene compounds, including ursolic and oleanolic acids were introduced as ZEA degradation agents. Conclusion: Z. multiflora could be recommended as a new botanical source, and ursolic and oleanolic acids could be introduced as new phytochemical compounds that degrade ZEA. %U https://ijvr.shirazu.ac.ir/article_6896_e18771c52f86221e0d737fbca5a7fc3d.pdf