%0 Journal Article %T LAMP assay for rapid diagnosis of cow DNA in goat milk and meat samples %J Iranian Journal of Veterinary Research %I Shiraz University %Z 1728-1997 %A Deb, R. %A Sengar, G. S. %A Singh, U. %A Kumar, S. %A Raja, T. V. %A Alex, R. %A Alyethodi, R. R. %A Prakash, B. %D 2017 %\ 06/01/2017 %V 18 %N 2 %P 134-137 %! LAMP assay for rapid diagnosis of cow DNA in goat milk and meat samples %K Cow %K Goat %K LAMP assay %K Meat %K Milk %R 10.22099/ijvr.2017.4099 %X Animal species detection is one of the crucial steps for consumer’s food analysis. In the present study we developed an in-house built loop-mediated isothermal amplification (LAMP) assay for rapid detection of adulterated cow DNA in goat milk/meat samples. The cow milk/tissue DNA in goat milk/meat samples were identified in the developed LAMP assay by either naked eye visualizing with SYBR Green I dyes or by detecting the typical ladder pattern on gel electrophoresis. This test can detect up to minimum 5% level of cow components admixed in goat milk/meat samples and can be completed within 1 h 40 min starting from DNA extraction from milk/meat samples and can be performed in a water bath. Developed LAMP methodology is simple; rapid and sensitive techniques that can detect adulterant like cow components in goat milk/meat are more accurate than other existing DNA based technologies. %U https://ijvr.shirazu.ac.ir/article_4099_33749f0271c58f2cf4d9b5c9da45608d.pdf