%0 Journal Article %T Detection and differentiation of wild-type and vaccine strains of canine distemper virus by a duplex reverse transcription polymerase chain reaction %J Iranian Journal of Veterinary Research %I Shiraz University %Z 1728-1997 %A Dong, X. Y. %A Li, W. H. %A Zhu, J. L. %A Liu, W. J. %A Zhao, M. Q. %A Luo, Y. W. %A Chen, J. D. %D 2015 %\ 06/01/2015 %V 16 %N 2 %P 172-175 %! Detection and differentiation of wild-type and vaccine strains of canine distemper virus by a duplex reverse transcription polymerase chain reaction %R 10.22099/ijvr.2015.3197 %X Canine distemper virus (CDV) is the cause of canine distemper (CD) which is a severe and highly contagious disease in dogs. In the present study, a duplex reverse transcription polymerase chain reaction (RT-PCR) method was developed for the detection and differentiation of wild-type and vaccine strains of CDV. Four primers were designed to detect and discriminate the two viruses by generating 638- and 781-bp cDNA products, respectively. Furthermore, the duplex RT-PCR method was used to detect 67 field samples suspected of CD from Guangdong province in China. Results showed that, 33 samples were to be wild-type-like. The duplex RT-PCR method exhibited high specificity and sensitivity which could be used to effectively detect and differentiate wild-type and vaccine CDV, indicating its use for clinical detection and epidemiological surveillance. %U https://ijvr.shirazu.ac.ir/article_3197_fd93c845a6a8c3574e15039df27e9a3a.pdf