%0 Journal Article %T Polymerase chain reaction for the detection and differentiation of Marek’s disease virus strains MDV-1 and HVT %J Iranian Journal of Veterinary Research %I Shiraz University %Z 1728-1997 %A Sadeghi, M. R. %A Ghorashi, S. A. %A Kargar Moakhar, R. %A Morshedi, D. %A Salehi Tabar, R. %A Ghaemmaghami, S. S. %D 2006 %\ 03/01/2006 %V 7 %N 1 %P 17-21 %! Polymerase chain reaction for the detection and differentiation of Marek’s disease virus strains MDV-1 and HVT %K Marek’s disease virus %K PCR %K Detection %R 10.22099/ijvr.2006.2675 %X Marek’s disease (MD) is a lymphoproliferative disease of chickens characterized by lymphocyticinfiltration of various organs. The present study was an attempt to use polymerase chain reaction (PCR) tooptimize a rapid and reliable assay for detection of MDV genome. Detection of serotype 1 of MDV (MDV-1)was confirmed by presence of a 200 bp DNA fragment as a PCR product. Differentiation of MDV-1 andherpesvirus of turkeys (HVT) was also conducted using specific primers from the glycoprotein A (gA) geneand a 388 bp DNA fragment was amplified from HVT genome. The specificity of the test was confirmed bysequencing of PCR products. Results indicate that MDV-1 can be diagnosed in clinical samples and inoculated cell cultures which is used for virus isolation. In addition, differentiation between MDV-1 and HVT viruses was confirmed based on the size of PCR products. The test proved to be rapid and reliable and can be performed as a robust diagnostic test in veterinary diagnostic laboratories. %U https://ijvr.shirazu.ac.ir/article_2675_04cdb1d922cc0ba55fd73aea7a1bb43e.pdf