%0 Journal Article %T The efficacy of inactivated oil-emulsion H9N2 avian influenza vaccine %J Iranian Journal of Veterinary Research %I Shiraz University %Z 1728-1997 %A Moghaddam Pour, M. %A Momayez, R. %A Akhavizadegan, M. A. %D 2006 %\ 06/01/2006 %V 7 %N 2 %P 85-88 %! The efficacy of inactivated oil-emulsion H9N2 avian influenza vaccine %K Avian influenza H9N2 %K Inactivated oil-emulsion vaccine %K Efficacy %R 10.22099/ijvr.2006.2669 %X An experimental inactivated oil-emulsion H9N2 avian influenza vaccine was formulated with 3 parts ofinactivated avian influenza antigen A/Chicken/Iran/101/1998(H9N2) emulsified in 7 parts of oil adjuvant.Twelve week-old specific pathogen-free (SPF) chickens were divided into seven groups of 10 birds. Sixgroups were vaccinated with 1, 1/10th, 1/50th, 1/100th, 1/200th and 1/400th field dose of the experimentalavian influenza vaccine (EAIV). The last group, was injected with saline and served as the control group.The mean titer in haemagglutination inhibition (HI) test (log 2) on the vaccinated groups, 21 days postvaccination were 6.0, 4.4, 3.83, 3.3, 3.0 and 2, respectively. Prevention of virus shedding through cloaca was used as the potency test which revealed that the protective doses 50% (PD50) of full, 1/10th and 1/50th of the field dose of the experimental vaccine were 100, 100 and 96.25%, respectively. Those groups thatreceived <1/50th dose could not prevent virus shedding. So it can be concluded that EAI vaccine could evenbe entirely protective and efficient in 1/10th dose and got a desirable immunity in experimental SPFchickens. %U https://ijvr.shirazu.ac.ir/article_2669_d98a4c4f11ae7948b610d5ca4181e05a.pdf