@article { author = {Sadeghi, M. R. and Ghorashi, S. A. and Kargar Moakhar, R. and Morshedi, D. and Salehi Tabar, R. and Ghaemmaghami, S. S.}, title = {Polymerase chain reaction for the detection and differentiation of Marek’s disease virus strains MDV-1 and HVT}, journal = {Iranian Journal of Veterinary Research}, volume = {7}, number = {1}, pages = {17-21}, year = {2006}, publisher = {Shiraz University}, issn = {1728-1997}, eissn = {2252-0589}, doi = {10.22099/ijvr.2006.2675}, abstract = {Marek’s disease (MD) is a lymphoproliferative disease of chickens characterized by lymphocyticinfiltration of various organs. The present study was an attempt to use polymerase chain reaction (PCR) tooptimize a rapid and reliable assay for detection of MDV genome. Detection of serotype 1 of MDV (MDV-1)was confirmed by presence of a 200 bp DNA fragment as a PCR product. Differentiation of MDV-1 andherpesvirus of turkeys (HVT) was also conducted using specific primers from the glycoprotein A (gA) geneand a 388 bp DNA fragment was amplified from HVT genome. The specificity of the test was confirmed bysequencing of PCR products. Results indicate that MDV-1 can be diagnosed in clinical samples and inoculated cell cultures which is used for virus isolation. In addition, differentiation between MDV-1 and HVT viruses was confirmed based on the size of PCR products. The test proved to be rapid and reliable and can be performed as a robust diagnostic test in veterinary diagnostic laboratories.}, keywords = {Marek’s disease virus,PCR,Detection}, url = {https://ijvr.shirazu.ac.ir/article_2675.html}, eprint = {https://ijvr.shirazu.ac.ir/article_2675_04cdb1d922cc0ba55fd73aea7a1bb43e.pdf} }