0.05). Using four sets of primers, a quadruplex PCR was developed to detect genes (nor1, ver1, omtA and aflR) at different loci coding enzymes in the aflatoxin biosynthesis pathway of A. flavus and A. parasiticus strains. Out of 28 strains of A. flavus and A. parasiticus, 10 isolates (35.71%) showed a quadruplet pattern indicating the important genes involved in the aflatoxin biosynthesis pathway, encoded for functional products. These isolates were confirmed to be aflatoxigenic by Thin Layer Chromatography. 18 isolates (64.29%) had three, two and single molecular patterns. The results obtained by this study show that rapid and specific detection of aflatoxigenic molds is important to ensure the microbiological safety of feedstuffs.]]> 0.1) by any dietary treatment. There were also no effects (P>0.1) of dietary treatment on body weight gain and feed efficiency except for T3 group, where body weight gain was lower compared with other treatments, and feed efficiency was lower than C2, T1, and T2. The metabolizability of dry matter tended (P=0.08) to decrease in T1, T2 and T3 groups than in C1 and C2 groups. Apparent metabolizable energy contents were significantly greater (P<0.05) in the C2 group than in the C1 group, but were similar among C1, T1, T2 and T3 groups. Themetabolizability of fat and other nutrients were not affected (P>0.10) by dietary treatments. Major carcass traits were unaffected (P>0.10) among the treatments. In conclusion, soybean oil and palm oil with GPGR as emulsifier could be added in the diets containing high amount of rice bran without affecting the performance; whereas lard may adversely affect the performance of ducks.]]> 0.05). The results of this study demonstrate that chick AF can enhance peripheral nerve regeneration.]]> 0.05). For the bull considered as control, the fertilization rate was 72.2 ± 4.5%, differing significantly (P>0.05) from the frozen/thawed epididymal semen’s fertilization rate. In conclusion, it is possible to use in vitro techniques with cryopreserved spermatozoa obtained from bull’s epididymis using a controlled rate freezing method with a predetermined freezing curve, and with assessment of sperm’s viability by conventional techniques and flow cytometry methods, together with the fertilizing ability of cryopreserved epididymal spermatozoa.]]> 0.05). Coxiella burnetii seroprevalence in yaks in different age groups ranged from 10.88% to 15.26%, but the difference was not statistically significant (P>0.05). Coxiella burnetii seroprevalence in yaks sampled in different seasons ranged from 12.06% (autumn) to 18.33% (summer), but the difference was not statistically significant (P>0.05). This is the first report of C. burnetii seroprevalence in free-range yaks in China, indicating the need for measures to be taken to control C. burnetii infection in free-range yaks in China.]]>