F. Mukherjee Research & Development Laboratory, National Dairy Development Board, Gachibowli, Hyderabad 500032, Telangana, India author A. Prasad Research & Development Laboratory, National Dairy Development Board, Gachibowli, Hyderabad 500032, Telangana, India;Department of Bio-Technology, Jawaharlal Nehru Technological University, Hyderabad 500032, Telangana, India author V. S. Bahekar Research & Development Laboratory, National Dairy Development Board, Gachibowli, Hyderabad 500032, Telangana, India author S. K. Rana Research & Development Laboratory, National Dairy Development Board, Gachibowli, Hyderabad 500032, Telangana, India author L. Rajendra Research and Development, Santha Biotechnniques (AS Sanofi Company), Athivelly PlotNo. 4, Medchal, Ranga Reddy District, Hyderabad-501401, India author G. K. Sharma Animal Health, National Dairy Development Board, Anand, 388001, Gujarat, India author V. A. Srinivasan Animal Health, National Dairy Development Board, 33 Telecom Nagar, Gachibowli, Hyderabad 500032, Telangana, India author text article 2016 eng The use of liposome as an adjuvant and a vaccine carrier has been cited previously in the literature. It has also been shown to be effective in enhancing the immunogenicity of vaccine candidates. BALB/c mice immunized subcutaneously with outer membrane protein (OMP) of Brucella abortus S19 vaccine strain entrapped in a commercial cationic liposome (S19-OMP-liposome) for vaccine delivery, showed enhanced protection (P<0.05) compared to groups of mice inoculated with S19 OMP alone, S19 live B. abortus vaccine and liposome alone, when challenged intra-peritoneally with virulent B. abortus strain 544 at 30 days post-immunization (DPI). The S19-OMP-liposome preparation was found to be safer compared to the live B. abortus S19 vaccine at 15 days post challenge (DPC), as evidenced by the significant difference in spleen weight between S19-OMP-liposome, S19 OMP and S19 live as well as the liposome control groups (P<0.01). Antibody isotype response profiles of the experimental groups indicated that the immune response was Th1 cell mediated. The protective advantage conferred to mice immunized with S19-OMP entrapped in liposome over those immunized with the live B. abortus S19 version, could probably be related to the significantly different response of IgG2b at 30 DPI (P<0.01), IgG2a (P<0.01), IgG2b (P<0.01) and IgG3 (P<0.05) at the DPC stages, respectively. معاونت پژوهشی‌ دانشگاه شیراز 1728-1997 17 v. 1 no. 2016 1 7 https://ijvr.shirazu.ac.ir/article_3594_3990bd32e60f9f6bc977e1a43bc8fe6f.pdf dx.doi.org/10.22099/ijvr.2016.3594 A. Khorasani Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran author O. Madadgar Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran author H. Soleimanjahi Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran author H. Keyvanfar Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran author H. Mahravani Department of FMD Vaccine Production, Razi Vaccine and Serum Research Institute, Karaj, Iran author text article 2016 eng Foot-and-mouth disease is an important viral disease of cloven-hoofed animals. Inactivated whole particle virus vaccines are still widely used in prophylactic vaccination campaigns. The choice of adjuvant is a very important factor in enhancing immune responses and the efficacy of inactivated vaccines. Montanide ISA 61 VG is a new ready-to-use mineral oil-based adjuvant developed by SEPPIC Inc. (SEPPIC, France) with high-potential immune responses needed for clinical protection against FMD infection. In this study, we compared the efficacy of two FMD vaccines either formulated with the new oil-based adjuvant ISA 61 VG and saponin, or with aluminum hydroxide gel and saponin. Both vaccines contained the same antigen payloads of O2010/IR. Two groups of 15 naive cattle received a single vaccination with different doses (full dose, 1/3 dose and 1/9 dose) to calculate their PD50 (50% protective dose) after being challenged with the homologous virulent virus. The mean neutralizing antibody titer was determined at 0, 7, 14 and 21 days after vaccination, measured by a micro neutralization test. The new vaccine improved humoral immune responses by 19%, while inducing a higher geometric mean. The titer for neutralizing antibodies was 2.91 log10 compared to the alum-gel based adjuvant vaccine which was 2.44 log10 (P-value=0.1782). The new vaccine showed a PD50 value of 10.05 as compared to a PD50 value of 4.171, respectively. According to the results, the FMD vaccine formulated with the new oil adjuvant, ISA 61 VG, shows potential as an alternative vaccine for routine and emergency vaccinations in the FMD enzootic region. معاونت پژوهشی‌ دانشگاه شیراز 1728-1997 17 v. 1 no. 2016 8 12 https://ijvr.shirazu.ac.ir/article_3596_70de8153db59e0242fb445e54cbf7325.pdf dx.doi.org/10.22099/ijvr.2016.3596 G.C. Okpe Department of Veterinary Anatomy, University of Nigeria, Nsukka, 410001, Nigeria author D.N. Ezeasor Department of Veterinary Anatomy, University of Nigeria, Nsukka, 410001, Nigeria author text article 2016 eng Quantitative histology of the descended testis of unilateral cryptorchid bucks was compared with testis of normal bucks to evaluate the reproductive potentials of the scrotal testis in unilateral cryptorchids, using light microscopy techniques. The contralateral scrotal testes of the unilateral cryptorchids and the testes of the normal bucks contained profiles of seminiferous epithelium and each showed histological evidence of normal activity. The mean heights, lengths, lumen diameter, diameter of the seminiferous tubules were significantly higher in the contralateral scrotal testes when compared to the retained testes of the unilateral cryptorchid bucks (P<0.05). Population of spermatogenic cells per testis, and ratio of germ cells to Sertoli cells were not significantly different between both groups. The percentage of the testes occupied by various germ cells did not differ between the scrotal testis of the cryptorchid bucks and those of the normal bucks. The volume occupied by the seminiferous tubules and Leydig cells in the contralateral scrotal testis of the unilateral cryptorchid bucks were significantly greater than those of the testis of normal bucks (P<0.05). From the findings, it appears that the spermatogenic efficiency of the scrotal testes of the unilateral cryptorchid bucks was significantly higher than those of the normal bucks. معاونت پژوهشی‌ دانشگاه شیراز 1728-1997 17 v. 1 no. 2016 13 19 https://ijvr.shirazu.ac.ir/article_3597_1d30c5602f23d765e58b41cd9a511c3a.pdf dx.doi.org/10.22099/ijvr.2016.3597 C. Debnath Department of Veterinary Public Health, Faculty of Veterinary and Animal Sciences, West Bengal University of Animal and Fishery Sciences (WBUAFS), 37, K.B. Sarani, Belgachia, Kolkata-700 037, West Bengal, India author T. Mitra Department of Veterinary Public Health, Faculty of Veterinary and Animal Sciences, West Bengal University of Animal and Fishery Sciences (WBUAFS), 37, K.B. Sarani, Belgachia, Kolkata-700 037, West Bengal, India author A. Kumar Veterinary Public Health Division, Indian Veterinary Research Institute (IVRI), Izatnagar, Bareilly, Uttar Pradesh-243 122, India author I. Samanta Department of Veterinary Microbiology, Faculty of Veterinary and Animal Sciences, West Bengal University of Animal and Fishery Sciences (WBUAFS), 37, K.B. Sarani, Belgachia, Kolkata-700 037, West Bengal, India author text article 2016 eng In recent times increasing occurrence of dermatophytosis, especially among the school children in eastern India was evidenced along with increased tendency of keeping companion animals such as dogs and cats. This study was undertaken to detect the occurrence of dermatophytes with antifungal susceptibility among the companion animals. A total of 1501 healthy companion animals comprising 1209 dogs and 292 cats belonged to individual owners in and around Kolkata (West Bengal, India) were examined for the evidence of dermatophytosis during 2011-2013. The collected samples were subjected to direct examination by standard KOH mount technique. The samples were inoculated into both Sabouraud dextrose agar (SDA) with 0.05% chloramphenicol and 0.5% cycloheximide and dermatophyte test medium (DTM). Each of the fungal isolate was identified based upon its colony characteristics and hyphal and conidial cells it produced. Antifungal susceptibility of the isolates was tested by broth micro dilution assay using fluconazole, ketoconazole, itraconazole, miconazole, griseofulvin and amphotericin-B antifungals. Among the 1209 samples from dogs and 292 samples from cats, 253 (20.93%) and 109 (37.33%) samples were positive for dermatophytes by direct examination. Three identified species of dermatophytes with predominant occurrence were Microsporum canis, Microsporum gypseum and Trichophyton mentagrophytes. Ketoconazole (0.06-0.5 μgm/ml), itraconazole (0.03-0.5 μgm/ml) and amphotericin-B (0.03-0.5 μgm/ml) showed lowest MIC values against M. canis, T. mentagrophytes and M. gypseum, respectively. This is the first systemic report of dermatophytes in healthy companion animals with large numbers of samples in India. معاونت پژوهشی‌ دانشگاه شیراز 1728-1997 17 v. 1 no. 2016 20 24 https://ijvr.shirazu.ac.ir/article_3598_519f72c97c794ffdc510ac96a54ea4c1.pdf dx.doi.org/10.22099/ijvr.2016.3598 K. Hoseini Pajooh Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran author P. Tajik Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran author M. Karimipoor Molecular Medicine Depart-ment, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran author M. Behdani Molecular Medicine Depart-ment, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran author text article 2016 eng Sperm mediated gene transfer can be an inexpensive and simple method in animal transgenesis; however its efficiency is poor, mainly due to the spermatozoa’s lesser uptake of exogenous DNA. In the present study, the effects of lipofection and other augmentation techniques, such as sperm freezing and spermatozoa treatment with triton X100 and DMSO, on exogenous DNA uptake by sheep spermatozoa and motility of sperms with plasmid uptake were evaluated. In the first experiment, ram sperms were incubated with a complex of rhodamine labeled plasmid (p-EGFP) and Lipofectamine 2000TM. In the second, spermatozoa were treated with Triton X-100TM or DMSO or were frozen without cryoprotectant. The results indicated that there was no significant difference (P<0.05) in the transfection rates and in the uptake intensity of lipofected sperms with 300 and 600 ng of plasmid in comparison with control group, i.e. transfected without lipofectamine. Furthermore, lipofection could not improve sperm motility during true plasmid uptake. Almost all of triton X100 treated and frozen-thawed spermatozoa had absorbed foreign DNA, though all were immotile. In spermatozoa treated with 0.1% DMSO, plasmid absorption rate (69.40%) was significantly higher (P<0.05) than untreated spermatozoa (57.80%), but sperm motility was not significantly different from control group. In conclusion, lipofectamine® 2000 could neither improve transfection rate, nor support motility in transfected sperms. The methods inducing membrane disruption like, freeze-thaw and triton X100 treatment, can be used in ICSI-sperm mediated gene transfer without the need for sperm selection, provided that they cause no damage to sperm nucleus. معاونت پژوهشی‌ دانشگاه شیراز 1728-1997 17 v. 1 no. 2016 25 30 https://ijvr.shirazu.ac.ir/article_3599_60d77ab1522efbb4667cacf4d45df3ef.pdf dx.doi.org/10.22099/ijvr.2016.3599 Z. Mozhdeganloo Post-Graduate Student, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran author A. Moghadam Jafari Department of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran author M.K. Koohi Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran author M. Heidarpour Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran author text article 2016 eng The objective of this study was to investigate the propensity of permethrin (PTN) to induce oxidative stress and changes in enzyme activities in liver of rainbow trout and its possible attenuation by vitamin C. Forty-eight fish were randomly assigned to 1 of 6 treatment groups and their livers were used for liver perfusion method: control (0 μgL-1 permethrin and 0 mgL-1 vitamin C), PTN-0.16 (0.16 μgL-1 permethrin), PTN-0.32 (0.32 μgL-1 permethrin), PTN-0.64 (0.64 μgL-1 permethrin), Vit. C (17.2 mgL-1 vitamin C), and PTN-0.64 + Vit. C (0.64 μgL-1 permethrin and 17.2 mgL-1 vitamin C). Results obtained showed that permethrin significantly (P<0.05) increased ALT, AST and LDH activities in the liver perfusion medium and malondialdehyde (MDA) level in liver tissue. The values of reduced glutathione (GSH) and total antioxidant capacity (FRAP) in the liver tissue were significantly decreased due to permethrin administration. Pearson’s correlation analysis revealed a positive correlation between MDA concentration and ALT, AST and LDH activities in the permethrin groups, suggesting that the enhanced lipid peroxidation may be linked to hepatic damage caused by permethrin. On the other hand, treatment with vitamin C in the PTN-0.64 + Vit. C group increased the values of GSH and FRAP, and decreased the level of MDA and the activities of hepatic enzymes, when compared to the PTN-0.64 group. The present study revealed that vitamin C could ameliorate permethrin-induced oxidative damage by decreasing lipid peroxidation and altering antioxidant defense system in liver of rainbow trout. معاونت پژوهشی‌ دانشگاه شیراز 1728-1997 17 v. 1 no. 2016 31 35 https://ijvr.shirazu.ac.ir/article_3600_f82934e264301c823bd8a66a0613dd67.pdf dx.doi.org/10.22099/ijvr.2016.3600 B. Mosallanejad Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran author R. Avizeh Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran author M. Razi Jalali Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran author M. Pourmahdi Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran author text article 2016 eng The purpose of the present survey was to determine the effects of the chitosan and atorvastatin on serum lipid profile changes and the influence of time on treatment process in cats. For the management of cholesterol induced hyperlipidemia, twenty-one healthy cats were randomly divided into three equal groups. Group A (control) included seven cats that were fed with cholesterol powder (4 g/kg for 10 days). Group B was similar to group A, but in addition, atorvastatin (5 mg/kg) was administered for 45 days after induced hyperlipidemia. Group C was similar to group B, but chitosan (3 g/cat) was administered instead of atorvastatin. Blood samples were collected four times on days 0, 10, 40 and 55 after challenge. Serum total cholesterol, triglycerides, HDL-C and LDL-C levels were measured using standard commercial kits. Atorvastatin (P<0.001) and chitosan (P<0.01) showed more hypolipidemic activity in lowering triglycerides compared with group A. In a comparison between two drugs and their effects on triglyceride, atorvastatin showed a significant difference with chitosan (P<0.01). Atorvastatin (P<0.01) and chitosan (P<0.05) showed more activity in lowering cholesterol than the control group. The treated groups (B and C) had good results in lowering LDL-C, compared with group A, on day 45 (P<0.001). A significant difference was seen only between groups A and B and on day 45 in increase of HDL-C (P<0.01). In conclusion, it was shown that although both drugs had hypolipidemic activity in cats, atorvastatin was more effective than chitosan. Further experimentation will be needed to elucidate the possible biochemical mechanism of the drugs. معاونت پژوهشی‌ دانشگاه شیراز 1728-1997 17 v. 1 no. 2016 36 40 https://ijvr.shirazu.ac.ir/article_3601_b5018d375bc42a646d604d8374dc9559.pdf dx.doi.org/10.22099/ijvr.2016.3601 A. Kılıç Department of Microbiology, Sivrice Vocational High School, University of Firat, 23119 Elazığ, Turkey author H. Kalender Department of Bioengineering, Faculty of Engineering, University of Firat, 23119 Elazığ, Turkey author O. Koç Department of Microbiology, Veterinary Control Institute, 23200 Elazığ, Turkey author Ü. Kılınç Department of Microbiology, Veterinary Control Institute, 23200 Elazığ, Turkey author B. Irehan Department of Microbiology, Veterinary Control Institute, 23200 Elazığ, Turkey author M. Berri UMR Infectiologie et Santé Publique (UMR ISP 1282), Site 311, Route de Crotelles, 37380 Nouzilly, France author text article 2016 eng Q fever is a zoonotic disease that occurs worldwide and is caused by the obligate intracellular bacterium Coxiella burnetii. The aim of this study was to investigate the presence of C. burnetii infection in aborted sheep in eastern Turkey using PCR. A total of 200 fetuses were collected from aborted sheep belonging to 200 herds in different locations in the eastern part of Turkey. Foetal organ samples such as liver, spleen, lung and stomach were taken and the DNA was purified from two hundred pooled samples. PCR analysis of C. burnetii presence in infected organs was performed, and 4 samples (2%) were found positive. In addition, the pooled organ suspensions were inoculated to embryonated chicken eggs, and PCR analysis of yolk sacs showed C. burnetii DNA in 5 samples (2.5%). This study shows that C. burnetii infection has an important role in sheep abortions in eastern Anatolia region. معاونت پژوهشی‌ دانشگاه شیراز 1728-1997 17 v. 1 no. 2016 41 44 https://ijvr.shirazu.ac.ir/article_3602_b581fd7f93269ca97f721578f8aaaa1a.pdf dx.doi.org/10.22099/ijvr.2016.3602 S. Bernardi Latin American Center of Studies Dairy Problematic (CLEPL), Faculty of Veterinary Science, National University of Rosario, Rosario, Province of Santa Fe, Argentina author A. Rinaudo Latin American Center of Studies Dairy Problematic (CLEPL), Faculty of Veterinary Science, National University of Rosario, Rosario, Province of Santa Fe, Argentina author P. Marini Latin American Center of Studies Dairy Problematic (CLEPL), Faculty of Veterinary Science, National University of Rosario, Rosario, Province of Santa Fe, Argentina author text article 2016 eng The present study was carried out to characterize the cervical mucus (CM) collected when inseminating Holstein cows and to relate the secretion pattern with pregnancy. The mucus was collected from mid-cervix of 64 cows with spontaneus estrus (SE) and induced estrus (IE). The quantity, pattern and consistency of the mucus, pH, arborization patterns and the sperm motility were observed. The levels of progesterone and estradiol 17β in serum were also determined. Pregnancy detection was performed by means of transrectal ultrasonography 60 days after insemination. Mucous secretion of cows with SE was significantly different from those with IE, showing a lower degree of crystallization (SE 2.00; IE 2.75) and a lower level of steroid hormones (P4: SE 0.17 ng/ml and IE 0.33 ng/ml (t 1.99547 α 0.05); E2: SE 30.95 pg/ml and IE 47.76 pg/ml (t 1.99495 α 0.05). Progesterone level was significantly lower and estrogen significantly higher in pregnant cows (P) in relation to that observed in non pregnant females (N) (P4: P 0.20 ng/ml and N 0.44 ng/ml (t 1.99602 α 0.05); E2: P 54.77 pg/ml and N 40.75 pg/ml (t 1.99505 α 0.05). In conclusion pregnancy was associated with acopious, clear and watery discharge (similar to egg white), with an arborization degree of 2.25, presence of atypical fern leaves together with rosette formations and needles or thorns on rails due to low levels of progesterone accompanied by high concentrations of estrogens. معاونت پژوهشی‌ دانشگاه شیراز 1728-1997 17 v. 1 no. 2016 45 49 https://ijvr.shirazu.ac.ir/article_3603_ecb453b53c29f764d1a13e4d235feeb6.pdf dx.doi.org/10.22099/ijvr.2016.3603 Ş. Tütüncü Department of Histology and Embryology, Faculty of Veterinary Medicine, Ondokuz Mayıs University, Samsun, Turkey author T. İlhan Department of Histology and Embryology, Faculty of Veterinary Medicine, Uludag University, Bursa, Turkey author N. Özfiliz Department of Histology and Embryology, Faculty of Veterinary Medicine, Uludag University, Bursa, Turkey author text article 2016 eng Red hot pepper is a plant that belongs to the Solanaceae family and is known as Capsicum annuum. Capsaicin is the active ingredient of cayenne pepper. Ghrelin is a hormone, which consists of polypeptide structure. Ghrelin also contributes to growth hormone secretion, energy balance, food intake and body weight regulator. The aim of this study was the localization and expression of ghrelin in the ovaries of rats treated with capsaicin during the postnatal development. Ninety female Sprague-Dawley rats (21 d) were used. The rats were randomly divided into 3 groups (n=30 each) as pubertal, post pubertal and adult. Each group was subdivided into three groups. The first subgroup (control) was given no injections. The second subgroup (vehicle) received only 0.3 cc solvent and the third subgroup (experiment) received subcutaneous injection of equal volume of capsaicin (1 mg/kg/d) for 42, 56, and 70 days. Ghrelin immunoreactivity was determined in ovarian follicular granulosa cells, interstitial cells and corpus luteal cells. A ghrelin immunopositive reaction located in the cytoplasm of cells in all groups. These results indicate that prolonged administration of low dose capsaicin does not affect ghrelin expression. However, follicular atresia was seen in lower rate in capsaicin treated group in comparison to other groups. معاونت پژوهشی‌ دانشگاه شیراز 1728-1997 17 v. 1 no. 2016 50 54 https://ijvr.shirazu.ac.ir/article_3604_517a6bbd8fd072c08f77fa1e49e80187.pdf dx.doi.org/10.22099/ijvr.2016.3604 H. Homayoun Post-Graduate Student, College of Basic Sciences, Jahrom Branch, Islamic Azad University, Jahrom, Iran author Sh. Zahiri Anatomy Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran author V. Hemayatkhah Jahromi Biology Department, College of Basic Sciences, Jahrom Branch, Islamic Azad University, Jahrom, Iran author A. Hassanpour Dehnavi Ph.D. Student in Anatomical Sciences, Anatomy Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran author text article 2016 eng The aim of this study was to examine the possible morphological and morphometric changes resulting from vitrification of embryos at the cleavage stage. In this study, 30 mice early-cleavage embryos at different stages of cleavage, resulting from in vitro fertilization (IVF) techniques, were examined before and after vitrification. Digital images were taken from embryos before and after vitrification. Zona pellucida thickness, differences in zona pellucida thickness, and diameter and volume of blastomeres and embryos as morphometric parameters and current rating of appearance of embryos as morphological parameters, have been studied. According to our findings, there were significant mean differences in all morphometric parameters of the two groups except in the zona pellucid thickness (P≤0.05). With regard to the morphological parameter, the decrease in embryo quality was observed but it was not significant. According to the results, although little quantitative change observed is not necessarily synonymous with harmful intracellular damage, it seems that it is better to examine vitrification method more accurately. Because by making subtle changes in concentration and type of consumed solutions or techniques used, the changes may be minimized. معاونت پژوهشی‌ دانشگاه شیراز 1728-1997 17 v. 1 no. 2016 55 58 https://ijvr.shirazu.ac.ir/article_3605_3cf4381c7eb8036c68a96c804f87b63a.pdf dx.doi.org/10.22099/ijvr.2016.3605 P. Tyrnenopoulou Post-Graduate Student, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 546 27 Thessaloniki, Greece author M. Karayannopoulou Department of Clinical Studies, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 546 27 Thessaloniki, Greece author S. Angelopoulou Veterinary Clinic, Athens Horse Racing Track, Markopoulo Mesogaias 190 03, Athens, Greece author A. Pyrros Veterinary Clinic, Athens Horse Racing Track, Markopoulo Mesogaias 190 03, Athens, Greece author E. Mparous Veterinary Clinic, Athens Horse Racing Track, Markopoulo Mesogaias 190 03, Athens, Greece author G. Koliakos Department of Biochemistry, Medical School, Faculty of Health Sciences, Aristotle University of Thessaloniki, 541 24 Thessaloniki, Greece author N. Diakakis Department of Clinical Studies, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 546 27 Thessaloniki, Greece author text article 2016 eng Carpal chip fractures are common causes of lameness in racehorses. Due to disadvantages in surgical management, adjuvant treatment modalities are usually necessary. Adipose-derived stem cells (ADSCs) have the potential to differentiate into other cell types including bone and cartilage cells. Adipose-derived stromal vascular fraction (SVF) is produced during ADSCs isolation from adipose tissue. The purpose of this report was to present the successful management of a grade III chip fracture in the right carpus of a 5-year-old Thoroughbred gelding by intra-articularly injected autologous SVF one month after the arthroscopic removal of the fracture. This treatment resulted in lameness improvement and short rehabilitation period to previous racing activities. High performance levels and no recurrent injuries were recorded during a twenty month follow-up period. معاونت پژوهشی‌ دانشگاه شیراز 1728-1997 17 v. 1 no. 2016 59 61 https://ijvr.shirazu.ac.ir/article_3606_8a7449bc831e1a3547ccce7589a4c9e8.pdf dx.doi.org/10.22099/ijvr.2016.3606 M.H. Kang Department of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University, Seoul 143-701, South Korea author H.M. Park Department of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University, Seoul 143-701, South Korea author text article 2016 eng Two dogs with generalized seizures were evaluated. The dogs were diagnosed with traumatic intracranial hemorrhages based on the history, neurological examinations, and magnetic resonance imaging (MRI) of the brain. Treatment was started with oxygen, prednisolone and anticonvulsant agents. No further seizure activity was observed after treatment in both dogs, however cushing reflex was detected in case 1 and a left-sided hemi-paresis was detected in case 2. Further supportive treatment with recombinant human granulocyte colony-stimulating factor (rhG-CSF) was attempted. No abnormal signs were noted in either of the dogs and no recurrence was noted 16 and 14 months later, in case 1 and 2, respectively. These cases indicate that a combination of rhG-CSF treatment with previous therapy could be used in dogs with traumatic brain injury. معاونت پژوهشی‌ دانشگاه شیراز 1728-1997 17 v. 1 no. 2016 62 65 https://ijvr.shirazu.ac.ir/article_3607_e928282bd3d3fca6bfbc02b56e697e94.pdf dx.doi.org/10.22099/ijvr.2016.3607