Essentiality of antioxidant vitamins for ruminants in relation to stress and reproduction
S.
Nayyar
Department of Veterinary Physiology and Biochemistry, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Gadvasu, Ludhiana 141004, Punjab, India
author
R.
Jindal
Department of Veterinary Physiology and Biochemistry, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Gadvasu, Ludhiana 141004, Punjab, India
author
text
article
2010
eng
The naturally occurring antioxidants: vitamin E, β-carotene and vitamin C are known to ameliorate theoxidative stress generated during various physiological and pathological conditions. The oxidative stress mayoccur at different sites, different times, and by different mechanisms in the animal system. Lower levels ofthe anti-oxidant vitamins are associated with poor fertility and production levels in ruminants. Theseantioxidants need to be replenished/regenerated or supplemented at appropriate timings in order to optimizethe ruminant health and productive/reproductive performance.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
11
v.
1
no.
2010
1
9
https://ijvr.shirazu.ac.ir/article_165_4704f43239d55c8de77e3a0901dc498a.pdf
dx.doi.org/10.22099/ijvr.2010.165
Effect of dexamethasone in combination with acetylcysteine at different times on corneal wound healing in dogs
M. A.
Gholipour
Graduated from School of Veterinary Medicine, Shiraz University, Shiraz, Iran
author
A. A.
Sarchahi
Department of Clinical
Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
author
A.
Meimandi Parizi
Department of Clinical
Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
author
M.
Eghtedari
Department of Ophthalmology
and Pathology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
author
text
article
2010
eng
The purpose of this study was to evaluate the effects of different time combinations of dexamethasoneand acetylcysteine on experimentally induced corneal ulcers in dogs. Experimental corneal wounds werecreated surgically to the anterior one third of the cornea in the center of all eyes of 15 mixed breed dogs. The eyes were divided into five groups according to planned post-operative medications: group 1, one drop of Nacetylcysteine 3% and one drop of dexamethasone 0.1% immediately after surgery; group 2, two drops of Nacetylcysteine 3% from day 1, one drop of N-acetylcysteine 3% and one drop of dexamethasone 0.1% from day 15; group 3, two drops of N-acetylcysteine 3%; group 4, two drops of dexamethasone 0.1%; group 5 (control), two drops of normal saline. When applied immediately after corneal ulceration, dexamethasone0.1% (group 4) decreased corneal haze significantly and did not delay corneal wound healing. Addition ofdexamethasone 0.1% to N-acetylcysteine 3% from day 15 (group 2) significantly suppressed opacity at twomonths after the beginning of the study, but when dexamethasone 0.1% associated to N-acetylcysteine 3%immediately after corneal ulceration (group 1), significant delay in corneal wound healing was induced. It isconcluded that combination of dexamethasone 0.1% and NAC 3% immediately after surgery may delaycorneal wound healing, also use of these drugs individually, has no obvious clinical effect on corneal haze.On the other hand, use of these drugs in combination with each other may reduce the corneal haze in latermonths after discontinuation of drugs. However, further studies using larger groups of animals are needed todemonstrate the effectiveness of these pharmacological modulators following experimentally induced corneal wounds in dogs.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
11
v.
1
no.
2010
10
17
https://ijvr.shirazu.ac.ir/article_169_ff69a135b4a032c1dd6eb9ba1796cc16.pdf
dx.doi.org/10.22099/ijvr.2010.169
Parasitic fauna of spiny eel Mastacembelus mastacembelus from Greater Zab river in Iraq
S. K. R.
BashÄ›
MSc in Parasitology, Department of Biology, College of Science Education, University of Salahaddin, Erbil, Iraq
author
S. M. A.
Abdullah
Department of Biology, College of Science Education, University of Salahaddin, Erbil, Iraq
author
text
article
2010
eng
A total of 128 spiny eels Mastacembelus mastacembelus (Mastacembelidae) were collected from theGreater Zab river at Iski Kalak town, west of Erbil city, Kurdistan region, Iraq, from July 2007 to the end ofJune 2008. The fish were examined for ecto and endo parasites. The study revealed the existence of 16species of parasites (with a total prevalence of 89.06%) including: two species of protozoans (Trypanosomasp. and Trichodina pediculus), one species of monogenetic trematodes (Mastacembelocleidusheteranchorus), six species of digenetic trematodes (Allocreadium transversale, Asymphylodoramacracetabulum, Pseudochetosoma salmonicola, Clinostomum complanatum, Diplostomum flexicaudum and D. spathaceum), two species of cestodes (Polyonchobothrium magnum and Ligula intestinalis), two species of nematodes (Agamospirura sp. and Procamallanus viviparus), one species of leech, crustacean and arachnoide (Cystobranchus mammillatus, Argulus foliaceus and Arrenurus sp. respectively). Five species of these parasites (T. pediculus, A. transversale, D. flexicaudum, C. mammillatus and Arrenurus sp.) wererecorded for the first time in Iraq. In addition, M. mastacembelus was regarded as a new host for A.macracetabulum, C. complanatum and A. foliaceus.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
11
v.
1
no.
2010
18
27
https://ijvr.shirazu.ac.ir/article_170_5c50b2a2d9fec518a9845b4d7edd98f0.pdf
dx.doi.org/10.22099/ijvr.2010.170
Compensatory ovarian changes, mast cell distribution and luminal structure changes following unilateral ovariectomy in rats
M.
Razi
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
author
S.
Feyzi
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
author
S.
Shamohamadloo
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
author
G.
Najafi
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
author
A.1
Ensafi
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
author
Sh.
Eyvari
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
author
T.
Peyrovi
Department of Basic Sciences, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran
author
text
article
2010
eng
To follow-up the present study, 40 mature female rats were used. The animals were divided into test,control and sham groups. The rats in the test group were laparotomized and unilateral ovariectomy was done. On the 1st and 2nd met-oestrous after operation, the tissue samples were dissected out and processed for histological study. The intact ovary in the test group underwent a significant (P<0.05) hypertrophy and compensatory changes including double angiogenesis, high cytoplasmic vacuolation in granulosa cell types of corpus luteum, compensatory follicular development, decreasing of follicular atresia, approximately double ovulation rate and increase in the width of cortex and medulla. The uterine horn on the intact side was approximately similar to the control and sham, while morphometric analyses showed that the horn on the ovariectomized side had significant decrease in the thickness of different layers. Scattering of endometrial glands on the uterine horn of the intact side was normal, showing a dense basophilic appearance in haematoxyline and eosin staining sections. The horn on the ovariectomized side showed low gland scattering. Histomorphometric analyses showed no significant differences between the vaginas in the three different groups. Distribution of mast cells (MCs) as essential cells participating in angiogenesis was investigated. In the test group, mast cells were considerably increased in number around the blood vessels in the medulla of the intact ovary and intact uterine horn. Mast cells were very low in number on the horn on the ovariectomized side.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
11
v.
1
no.
2010
28
37
https://ijvr.shirazu.ac.ir/article_171_2e15baf34adfccf3471a464f3eb0fd17.pdf
dx.doi.org/10.22099/ijvr.2010.171
The role of the histaminergic system on the inhibitory effect of ghrelin on feed intake in broiler chickens
M.
Taati
Department of Pathobiology, School of Veterinary Medicine, Lorestan University, Khorramabad, Iran
author
H.
Nayebzadeh
Department of Pathobiology, School of Veterinary Medicine, Lorestan University, Khorramabad, Iran
author
H.
Khosravinia
Department of Animal Sciences, Faculty of Agriculture, Lorestan University, Khorramabad, Iran
author
J.
Cheraghi
Department of Physiology, College of Veterinary Medicine, Ilam University, Ilam, Iran
author
text
article
2010
eng
This study was conducted to investigate the possible involvement of the histaminergic system in themediation of ghrelin-induced feeding behavior in broiler chickens. In the trial 1, the effect ofintracerebroventricular (ICV) injection of ghrelin on feed intake was examined in 3-h feed-deprived broilerchickens. In the trials 2 and 3, the chickens were received ICV chlorpheniramine (an H1 receptor antagonist)and cimetidine (an H2 receptor antagonist) prior to ghrelin injection, respectively. Cumulative feed intakewas measured at 3-h post injections. Infusion of ghrelin (0.3, 0.6 and 1.2 nmol) decreased feed intake dosedependently (P<0.05). Pre-treatment of chlorpheniramine (100 μg) attenuated the inhibitory effect of ghrelin on feed intake (P<0.05), while such an effect was not noticed by pre-injection of cimetidine (100 μg). The results suggest that there is an interaction between ghrelin and the histaminergic system (through H1receptors) on control of feed intake in broiler chickens.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
11
v.
1
no.
2010
38
45
https://ijvr.shirazu.ac.ir/article_173_8cb80bdb5ae5b06fb1a7a5793bbf8fdd.pdf
dx.doi.org/10.22099/ijvr.2010.173
Influence of antagonistic minerals in soil and pastures on the blood and liver copper in goats in Khuzestan province, Iran
A.
Rasooli
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
author
M.
Nouri
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
author
M.
Razi-Jalali
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
author
text
article
2010
eng
The copper level in the serum of 1280 and liver of 200 goats raised in the mountain areas of Khuzestanprovince, Iran were determined. Copper, molybdenum and iron content of soil and pastures as well as sulfurcontent of the pastures were seasonally estimated. Mean serum copper levels of goats in Behbahan,Ramhormoz, Eizeh and Masjed-Soleyman districts were 8.14 ± 0.21, 1.98 ± 0.12, 7.74 ± 0.11 and 6.97 ±0.14 μmol/L, respectively. The results showed that the blood copper of goats in the area was significantly(P<0.05) low in autumn and winter in comparison with spring and summer. The liver copper concentrationwas low in Behbahan, Eizeh and Masjed-Soleyman and at a deficient level in Ramhormoz. The results alsorevealed a high level of molybdenum in the soil and pasture as well as high amounts of sulfur in pastures ofthe studied areas. In this survey the status of copper in many goat flocks around the studied towns wasdangerously low. Many liver samples had a copper concentration below 30 mg/kg and many serum copperconcentrations were below normal. The results of this study confirm the presence of a secondary copperdeficiency in goats in the mountain areas of Khuzestan province.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
11
v.
1
no.
2010
46
50
https://ijvr.shirazu.ac.ir/article_174_341f7dbf95c8e6424c3bba5ed17161ea.pdf
dx.doi.org/10.22099/ijvr.2010.174
Central effect of mammalian oxyntomodulin on food intake in non-fasted and fasted chicks
A.
Ghazanfari Moghaddam
Department of Biology, College of Sciences, University of Tehran, Tehran, Iran
author
H.
Jonaidi
Department of Basic
Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman (SBUK), Kerman, Iran
author
M.
Abbasnejad
Department of Biology, Faculty of Basic Sciences, Shahid Bahonar University of Kerman (SBUK), Kerman,
Iran
author
M. M.
Yaghoobi
Department of Biotechnology, International Center for Science and High Technology and
Environmental Sciences, Mahan, Iran
author
H.
Sepehri
Department of Biology, College of Sciences, University of Tehran, Tehran, Iran
author
text
article
2010
eng
Oxyntomodulin (OXM), a proglucagon-derived peptide, is a well known anorexigenic peptide found inthe gut and brain of mammals. The present study was carried out to investigate the central effect of OXM onfood intake in non-fasted and fasted Ross broiler chicks. At four weeks of age, a guide cannula wasstereotaxically implanted into the right lateral ventricle of each bird. Two experiments were conducted onfree-feeding (non-fasted) and 6-h fasted broilers. In each experiment, eight birds were used in a replicated 4 × 4 Latin square design. The birds were given an intracerebroventricular (ICV) injection of different doses of mammalian OXM. Intracerebroventricular injection of OXM at doses of 1.5, 3 and 6 nmol significantly(P<0.05) decreased food intake for 3 h post injection in both non-fasted and fasted chicks. It may beconcluded that central OXM may exert a suppressive effect on food intake in chicks.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
11
v.
1
no.
2010
51
55
https://ijvr.shirazu.ac.ir/article_175_8b48ea7e5a6ad8b9ccdcb61f61777d68.pdf
dx.doi.org/10.22099/ijvr.2010.175
Hyperimmune lipopolysaccharide antiserum mediated inhibition of the adherence of E. coli O157:H7 to HEP-2 cells and large intestine of mice
S.
Moradi Bidhendi
Department of Microbiology, Razi Vaccine and Serum Research Institute, Karaj, Iran
author
P.
Khaki
Department of Microbiology, Razi Vaccine and Serum Research Institute, Karaj, Iran
author
M.
Sattari
Department of Bacteriology, School of Medicine, Tarbiat Modarres University, Tehran, Iran
author
M. H.
Heidari
Department of Anatomical
Sciences, Faculty of Medicine, Shahid Beheshty University of Medical Sciences, Tehran, Iran
author
text
article
2010
eng
Escherichia coli O157:H7 is found in cattle farms and can live in the intestine of healthy cattle. Mostcases of human illnesses including nonbloody diarrhea, hemorrhagic colitis and hemolytic uremic syndromecan be traced, either directly or indirectly, to cattle. One strategy for reducing the risk of EnterohemorrhagicEscherichia coli (EHEC) infections in human is to reduce the prevalence of infection in cattle. Antiserumagainst whole cell of isolated E. coli O157:H7 from cattle showed inhibition of adherence of this strain toHEP-2 cells and intestine of model mice. The ability of this antiserum in blocking the attachment of bacteriato HEP-2 cells in 1:1280 titer and to intestine tissue of mice in 1:640 titer are significant. Histology ofintestine tissue confirms our results. The difference between in vivo and in vitro titrations for blocking theattachment depends on these two different conditions.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
11
v.
1
no.
2010
56
63
https://ijvr.shirazu.ac.ir/article_176_aa997d48bf5e4707f48802be0ee3ad3d.pdf
dx.doi.org/10.22099/ijvr.2010.176
Effect of chronic hypoxia during the early stage of incubation on prenatal and postnatal parameters related to ascites syndrome in broiler chickens
S.
Bahadoran
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
M.
Hassanzadeh
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
A. K.
Zamanimoghaddam
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
author
text
article
2010
eng
To investigate the influence of hypoxia during the early stage of incubation on embryonic developmentand hatching events, and consequently on incidence of ascites in broiler chickens, one thousand fertile eggswere incubated in two commercial incubators. Half the eggs were incubated in a low altitude incubator untilhatched. The second half were incubated in a high altitude incubator until day 10 and then transferred to alow altitude incubator. Day-old chicks from each group were housed and reared at a high altitude farm.Chicks from the high altitude incubator hatched earlier and showed significantly higher body weights thantheir counterparts in the lower altitude. High altitude embryos indicated significantly (P<0.001) higherplasma corticosterone, T3 and T4 levels at day 10 and 19 of incubation. During the growing period, highaltitude hatched chickens indicated lower right ventricular hypertrophy and ascites mortality than the lowaltitude hatched chickens. These results indicated that early prenatal hypoxia due to high altitude may change the endocrine functions of embryos, enhanced embryo growth, shorten the hatching process of chickens and consequently decrease the incidence of ascites incidence in broiler chickens.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
11
v.
1
no.
2010
64
71
https://ijvr.shirazu.ac.ir/article_177_454000c46ff619e3621e8037367687d7.pdf
dx.doi.org/10.22099/ijvr.2010.177
Cloning and secretory expression of VP2 gene of infectious bursal disease virus in eukaryotic cells
Sh.
Ghafari
Graduated from Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran
author
M. R.
Seyfiabad Shapouri
Department of
Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
author
H.
Moatamedi
Department of Biology, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran
author
M.
Roayaei
Department of Biology, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran
author
H.
Goudarzi
Department of Avian Medicine, Razi Vaccine and Serum Research Institute, Karaj, Iran
author
text
article
2010
eng
VP2 gene coding region of a vaccinal strain (D78) of infectious bursal disease virus (IBDV) was clonedin a eukaryotic expression vector, pSec Tag2A. The gene was placed downstream of Ig κ chain leadersequence, under the control of human cytomegalovirus (hCMV) immediate early enhancer and promoter. Theconstruct pSec Tag2A-VP2 was transfected in COS-7 cell line and the expression and secretion of VP2 wasassessed by dot blotting and antigen capture ELISA. The antibody used in the immunological assays was aneutralizing monoclonal antibody (1A6) against VP2. Positive reaction with the antibody indicated theconstruct was functional with respect to expression and secretion of a native VP2.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
11
v.
1
no.
2010
72
77
https://ijvr.shirazu.ac.ir/article_178_93ec014a3b375cb0258bab9970ea4de6.pdf
dx.doi.org/10.22099/ijvr.2010.178
Selenium and iodine status of sheep in the Markazi province, Iran
A. R.
Talebian Masoudi
Agriculture and Natural Resource Research Center of Markazi Province, Arak, Iran
author
F.
Azizi
Research Institute for
Endocrine Sciences, Shahid Beheshti University (M.C), Tehran, Iran
author
H.
Zahedipour
Agriculture and Natural Resource Research Center of Markazi Province, Arak, Iran
author
text
article
2010
eng
The aim of this study was to provide preliminary quantitative information on selenium and iodine statusof sheep in Markazi province, Iran. Selenium and iodine status of grazing sheep were measured for 57different flocks in 14 regions over one year. The districts were selected to represent major sheep growingareas in the province. Blood samples were collected from 2 to 3-year-old ewes during summer, autumn andwinter. Samples were analyzed for blood glutathione peroxidase (GSHpx) activity and plasma inorganiciodine. There were significant differences between regions and seasonal differences in terms of blood GSHpxactivity and plasma inorganic iodine concentration (P<0.01). Low levels of GSHpx activity (<60 IU/gHb)and plasma inorganic iodine (<5 μg/dl) in some regions or some seasons indicated the need for dietarysupplementation of these minerals.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
11
v.
1
no.
2010
78
83
https://ijvr.shirazu.ac.ir/article_179_b5f800519fb78306ebb4a445fd947a95.pdf
dx.doi.org/10.22099/ijvr.2010.179
Bacteriological and serological studies on Mannheimia haemolytica infection in cattle slaughtered at Ahvaz (southwestern Iran) abattoir
M. R.
Haji Hajikolaei
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
author
M.
Ghorbanpour
Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of
Ahvaz, Ahvaz, Iran
author
M. R.
Seyfiabad Shapouri
Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of
Ahvaz, Ahvaz, Iran
author
A.
Rasooli
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
author
D.
Ebrahimkhani
Graduated from Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz,
Ahvaz, Iran
author
A. R.
Jabbari
Department of Microbiology, Razi Vaccine and Serum Research Institute, Karaj, Iran
author
text
article
2010
eng
In order to investigate the prevalence of Mannheimia haemolytica infection in cattle, nasal andnasopharyngeal swabs and blood samples were obtained from 250 cattle after slaughter at Ahvaz(southwestern Iran) abattoir. Nasal and nasopharyngeal swabs were cultured on blood agar and incubated at37°C for 24-48 h. The suspected bacterial cultures were processed for isolation of M. haemolytica followingroutine bacteriological techniques. Sera were tested by indirect hemagglutination test (IHA) to revealantibodies against the organism. M. haemolytica was isolated from 1.6% of the sampled cattle. Statisticalanalysis showed that there was no relationship between age and sex with bacterial infection. Serologicalstudies showed that 71.6% of tested sera contained antibody (titer≥1/16) against M. haemolytica. There wasno association between age and sex with serological results.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
11
v.
1
no.
2010
84
87
https://ijvr.shirazu.ac.ir/article_180_4b5e94365baa56c59ee6e84729cfd503.pdf
dx.doi.org/10.22099/ijvr.2010.180
Bacteriological study of dead-in-shell embryos of ostrich
text
article
2010
eng
In order to study the bacterial flora of dead-in-shell ostrich chicks, twelve unhatched eggs which did nothave external pipping during the hatching period were transferred to the laboratory of microbiology. The eggshells were accurately disinfected and the embryos were removed and placed in a sterile plate. The surface of each embryo was swabbed with a sterile swab which was also plunged through the yolk sac and the embryo contents and the swab were inoculated into tryptic soy broth (TSB) or nutrient broth. To enrich Salmonella spp., another swab was prepared as above and inoculated into Selenite-F broth. These media were incubated at 37°C for 24 h and then subcultured by streak plate method on solid media. Different bacterial colonies on solid media were isolated in pure cultures for further identification. The results of this study showed that the predominant bacterial flora of dead-in-shell embryos of ostrich were Bacillus spp. (45%) and Staphylococcus spp. (25%).
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
11
v.
1
no.
2010
88
90
https://ijvr.shirazu.ac.ir/article_181_b35fbf81b9bb05a7b52629015c1a3311.pdf
dx.doi.org/10.22099/ijvr.2010.181