1Department of Medical Parasitology and Mycology, School of Public Health and Institute of Public Health Researches, Tehran University of Medical Sciences, Tehran, Iran
2Faculty of Medicine, Hamedan University of Medical Sciences, Hamedan, Iran
3Laboratory of Bioinformatic, Biophysics-Biochemistry Research Center, University of Tehran, Tehran, Iran
4Faculty of Veterinary Medicine, University of Mazandaran, Amol, Iran
5Mycology Research Center, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Fusarium verticillioides (F. verticillioides) is not only a primary pathogen of maize, but also can cause disease in other crops such as sorghum. Pathogenicity is related to mycotoxin production such as fumonisin. In the present study, 24 isolates of F. verticillioides, which were previously identified by phenotype based methods, were re-identified using restriction fragment length polymorphism (RFLP) analysis. Digestion of the polymerase chain reaction (PCR) products with the restriction enzyme TasI allowed identifying four nonverticillioides strains that were discarded from our study. The genetic variations among the remaining 20 strains of F. verticillioides were analysed by random amplified polymorphic DNA (RAPD)-PCR method with 4 primers. Of the four primers tested, two primers produced polymorphic amplification patterns. Dendrogram for each primer indicated the distance of the strains to each other. Using primers of A, B, C and D, the isolates were divided to 8, 9, 7 and 7 groups, respectively. The results of this study indicated genetic relationship among DNA polymorphic patterns with geographic regions and the severity of fumonisin B1 (FB1) production. It seems that RAPD analysis is a suitable technique for strain typing of F. verticillioides.