Evolutionary dynamics of topotype ME-SA/Ind-2001 of foot and mouth disease virus serotype-O in Pakistan: 2017-2022

Document Type : Short paper

Authors

1 Ph.D. in Microbiology, Institute of Microbiology, Faculty of Veterinary Sciences, University of Veterinary and Animal Sciences, Lahore, Pakistan

2 Institute of Microbiology, Faculty of Veterinary Sciences, University of Veterinary and Animal Sciences, Lahore, Pakistan

3 Quality Operations Laboratory, Faculty of Veterinary Sciences, University of Veterinary and Animal Sciences, Lahore, Pakistan

4 Department of Biochemistry and Biotechnology, Faculty of Veterinary Sciences, University of Veterinary and Animal Sciences, Lahore, Pakistan

Abstract

Background: During past few years, the Ind-2001 lineage of the Middle East-South Asia topotype (ME-SA) of the foot-and-mouth disease (FMD) virus has been implicated in FMD outbreaks in Pakistan. Aims: This work conducts a comprehensive evolutionary analysis of the Ind-2001 and Pan Asia II lineages, with a specific emphasis on their geographical distribution, lineage classification, and sub-lineage distribution within the region. Furthermore, it aims to expand our understanding of the conserved region of the VP1 protein. Methods: Total samples (n=50) were subjected to antigen detection ELISA and RT-PCR for serotype determination. Confirmed serotype-O isolates (n=17) underwent sequencing for lineage comparison, mutation impact assessment on the VP1 protein GH loop, 3D structure prediction, and further comparative analysis. Results: Isolates collected from 2017 to 2020 were identified as serotypes O/ME-SA/Pan Asia II ANT10 and O/ME-SA/Pak14. Notably, isolates collected from 2020 to 2022 belonged to a novel FMDV serotype O/ME-SA/Ind-2001e lineage. Phylogenetic analyses indicated that these strains were distinct from dominant contemporaneous strains which may challenge Pakistan’s FMD control measures. These isolates exhibited variance in the VP1 epitope, specifically in amino acid residues 135-155, known to influence neutralizing antibody generation. Conclusion: Observed mutations suggest potential challenges to current vaccination efficacy against FMD. This emphasizes enhanced FMD surveillance and demonstrates that tracking the emergence of the O/ME-SA/Ind-2001e lineage is important for determining FMD control strategies in Asia.

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References

Abubakar, M; Syed, Z; Manzoor, S and Arshed, MJ (2022). Deciphering molecular dynamics of foot and mouth disease virus (fmdv): A looming threat to Pakistan’s dairy industry. Dairy. 13: 123-136. https://doi.org/10.3390/dairy3010010.
Arzt, J; Fish, I; Pauszek, SJ; Johnson, SL; Chain, PS; Rai, DK; Rieder, E; Goldberg, TL; Rodriguez, LL and Stenfeldt, C (2019). The evolution of a super-swarm of foot-and-mouth disease virus in cattle. PLoS ONE. 14: e0210847. https://doi.org/10.1371/journal.pone.0210847.
Bachanek-Bankowska, K; Di Nardo, A; Wadsworth, J; Mioulet, V; Pezzoni, G; Grazioli, S; Brocchi, E; Kafle, SC; Hettiarachchi, R and Kumarawadu, PL (2018). Reconstructing the evolutionary history of pandemic foot-and-mouth disease viruses: The impact of recombination within the emerging o/me-sa/ind-2001 lineage. Sci. Rep., 8: 14693. https://doi.org/10.1038/s41598-018-32693-8.
Brito, B; Rodriguez, L; Hammond, J; Pinto, J and Perez, A (2017). Review of the global distribution of foot-and-mouth disease virus from 2007 to 2014. Transbound. Emerg. Dis., 64: 316-332. https://doi.org/10.1111/tbed.12373.
Dahiya, SS; Subramaniam, S; Mohapatra, JK; Rout, M; Biswal, JK; Giri, P; Nayak, V and Singh, RP (2023). Foot-and-mouth disease virus serotype o exhibits phenomenal genetic lineage diversity in india during 2018-2022. Viruses. 15: 1529. https://doi.org/10.3390/ v15071529.
Depa, P; Dimri, U; Sharma, M and Tiwari, R (2012). Update on epidemiology and control of foot and mouth disease-a menace to international trade and global animal enterprise. Vet. World. 5: 694-704. doi: 10.5455/vetworld. 2012.694-704.
Di Nardo, A; Knowles, NJ; Wadsworth, J; Haydon, DT and King, DP (2014). Phylodynamic reconstruction of o cathay topotype foot-and-mouth disease virus epidemics in the philippines. Vet. Res., 45: 90. https://doi.org/10.1186/ s13567-014-0090-y.
Domingo, E; Baranowski, E; Escarmı́s, C and Sobrino, F (2002). Foot-and-mouth disease virus. Comp. Immunol. Microbiol. Infect. Dis., 25: 297-308. https://doi.org/10. 1016/S0147-9571(02)00027-9.
Haynie, T (2023). Recombinant DNA vaccine design as a potential strategy against bovine foot and mouth disease virus (fmdv). LSU Master’s Theses. 5772. doi: 10.31390/gradschool_theses.5772.
Jamal, SM; Ferrari, G; Ahmed, S; Normann, P and Belsham, GJ (2011). Genetic diversity of foot-and-mouth disease virus serotype o in Pakistan and Afghanistan, 1997-2009. Infect. Genet. Evol., 11: 1229-1238. https://doi.org/ 10.1016/j.meegid.2011.03.006.
Kanwal, S; Saeed, A; Munir, M and Arshad, M (2014). Phylogenetics of foot and mouth disease virus in punjab, Pakistan. Br. J. Virol., 1: 21-28.
Lee, G; Hwang, JH; Park, JH; Lee, MJ; Kim, B and Kim, SM (2020). Vaccine strain of o/me-sa/ind-2001e of foot-and-mouth disease virus provides high immunogenicity and broad antigenic coverage. Antivir. Res., 182: 104920. https://doi.org/10.1016/j.antiviral.2020.104920.
Li, D and Zhang, L (2022). Structure prediction and potential inhibitors docking of enterovirus 2c proteins. Front. Microbiol., 13: 856574. https://doi.org/10.3389/fmicb. 2022.856574.
Mahapatra, M and Parida, S (2018). Foot and mouth disease vaccine strain selection: Current approaches and future perspectives. Expert Rev. Vaccines. 17: 577-591. https://doi.org/10.1080/14760584.2018.1492378.
Mustafa, MZ; Kakar, MA; Abbas, F; Naudhani, S and Ahmad, J (2016). Multiplex one-step RT-PCR for detection and serotyping of foot and mouth disease virus in Balochistan. J. Appl. Sci., 5: 74-80. http://dx.doi.org/10. 36785/jaes.52137.
Park, JH; Lee, KN; Ko, YJ; Kim, SM; Lee, HS; Shin, YK; Sohn, HJ; Park, JY; Yeh, JY and Lee, YH (2013). Control of foot-and-mouth disease during 2010-2011 epidemic, south korea. Emerg. Infect. Dis., 19: 665. https://doi.org/10.3201%2Feid1904.121320.
Rizvi, A; Hussain, N; Anjum, AA; Ahmed, N; Naeem, A; Khan, M and Altaf, I (2022). Effect of cell density on the biological titer and yield of 146s fraction of foot-and-mouth disease virus o in cell suspension. J. Virol. Methods. 300: 114379. https://doi.org/10.1016/j.jviromet.2021.114379.
Ruiz-Sáenz, J; Goez, Y; Tabares, W and López-Herrera, A (2009). Cellular receptors for foot and mouth disease virus. Intervirology. 54: 201-212. https://doi.org/10.1159/ 000226121.
Singanallur, NB; Dekker, A; Eblé, PL; van Hemert-Kluitenberg, F; Weerdmeester, K; Horsington, JJ and Vosloo, W (2021). Emergency fmd serotype o vaccines protect cattle against heterologous challenge with a variant foot-and-mouth disease virus from the o/me-sa/ind2001 lineage. Vaccines. 9: 1110. https://doi.org/10.3390/ vaccines9101110.
Subramaniam, S; Mohapatra, JK; Sharma, GK; Biswal, JK; Ranjan, R; Rout, M; Das, B; Dash, BB; Sanyal, A and Pattnaik, B (2015). Evolutionary dynamics of foot-and-mouth disease virus o/me-sa/ind2001 lineage. Vet. Microbiol., 178: 181-189. https://doi.org/10.1016/j.vetmic. 2015.05.015.
Waheed, U; Parida, S; Khan, Q; Hussain, M; Ebert, K; Wadsworth, J; Reid, S; Hutchings, G; Mahapatra, M and King, D (2011). Molecular characterisation of foot-and-mouth disease viruses from Pakistan, 2005-2008. Transbound. Emerg. Dis., 58: 166-172. https://doi.org/ 10.1111/j.1865-1682.2010.01186.x.

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