Isolation of enterotoxigenic and enteroaggregative straines of Escherichia coli from chicken carcasses by PCR

Document Type: Short paper

Authors

1 Department of Health and Food Quality Control, Institute of Zoonoses Research, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran

2 Department of Food Hygiene, Faculty of Veterinary Medicine, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran

3 Graduated from Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran

Abstract

The aim of this study was to determine the frequency of enterotoxigenic and enteroaggregative strains of
Escherichia coli in chicken carcasses by polymerase chain reaction (PCR). In this study 63 strains of E. coli
were isolated from 110 samples of chicken carcasses during processing after chilling in the poultry slaughter
house of Shahrekord. Polymerase chain reaction assays were used to detect the presence of the genes
encoding heat-stable enterotoxin a (STa), heat-stable enterotoxin b (STb), heat labile toxin (LT) and
Enteroaggregative heat-stable toxin 1 (EAST1). Sixty three out of 110 (57.27%) carcasses were contaminated with E. coli. Six out of 63 (9.52%) harbored the gene for LT, 1 (1.58%) STb, 21 (33.3%) EAST1 and 8 (12.69%) contain both LT and EAST1 genes. None of the strains contain the STa gene. The results indicated that contamination of the chicken carcasses with E. coli in such a level could be a potential hazard for consumers.

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