Using various antigen preparations to produce monoclonal antibodies against bovine leukaemia virus (BLV) gp51SU

Document Type: Full paper (Original article)

Authors

1 Central Laboratory, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

2 Department of Pathobiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

Abstract

The objective of this study was to compare different antigen preparations to produce monoclonal
antibodies against bovine leukaemia virus gp51SU. The four antigen preparations for immunization of
BALB/c mice were: CL: BLV-FLK cell lysate, UF: a fraction of CL (between 30 and 100 kDa), WVP: whole
virus particles and SP: with ion exchange chromatography, gp51SU was semipurified. A total of nine
successful fusions were performed which resulted in production of 23 monoclonal antibodies (mAbs) specific
against gp51SU. The highest ratio of specific hybridoma colonies in each fusion was with SP preparation.
Based on the reactivity of the mAbs in Western blotting, mAbs were classified into four groups: anti-gp51SU
(23 mAbs), anti-gp30TM (8 mAbs), anti-Pr72 (5 mAbs) and antibodies against other viral proteins (7 mAbs).
Some of the anti-gp51SU mAbs reacted with more than one band in Western blotting, suggesting that these
colonies recognized not only gp51SU but also its precursors

Keywords