Phylogenetic analysis of canine parvovirus 2 subtypes from diarrheic dogs in Iran

Document Type : Short paper

Authors

1 Department of Clinical Sciences, Karaj Branch, Islamic Azad University, Karaj, Iran

2 Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, IranDepartment of Microbiology, Islamic Azad University, Karaj Branch

3 Department of Quality Control, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

Abstract

Background: Canine parvovirus type 2 (CPV-2) causes gastroenteritis and leukopenia in dogs worldwide. They are three subtypes of CPV-2 including CPV-2a, CPV-2b, and CPV-2c. The distribution status of CPV-2 subtypes has been shown differences in many countries. Aims: The aim of the present study was detection and phylogenetic analysis of different subtypes of CPV-2 circulating in two provinces of Iran, Tehran and Alborz. Methods: CPV-2 was detected using 555 primer pairs in collected samples. Phylogenetic analysis of CPV-2 subtypes was done using sequencing of the partial length of VP2 gene. Results: Twenty-eight CPV-2 were detected using 555 primer pair. The sequences of isolates were deposited in the GenBank database. Phylogenetic analysis revealed that all CPV-2c subtype isolates had very high sequence identity to China and Zambia that form a distinct cluster. Conclusion: In conclusion, this study revealed the emergence of all CPV-2 variants in dogs in Iran. Thus, the continual monitoring of CPV-2 in domestic dogs should be further conducted on a large scale to determine the predominant variants and their distributions in the country and to follow the dynamics of CPV-2 in the Middle East region of Asia.

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References

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