College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Eastern Hua Lan Street, 453003 Xinxiang, China
10.22099/ijvr.2020.37714.5508
Abstract
Background: Mammary epithelial cells have been widely-used over the years as models to understand the physiological function of mammary disease. Aims: This study aimed to establish a culture system and elucidate the unique characteristics of bovine mammary epithelial cells (BMEC) from the milk of Ukraine Holstein dairy cows in order to develop a general in vitro model. Methods: The milk from a three year old lactating dairy was used as a source of the epithelial cell, Characteristics of BMECs were examined using real-time cell assay (RTCA), immunocytochemistry (ICC), Reverse Transcription-Polymerase Chain Reaction (RT-PCR) and Westren blot (WB). Results: The results showed that bovine mammary epithelial cells can be recovered from milk, grown in culture, and exhibit the characteristic cobblestone morphology of epithelial cells. Conclusion: The established BMECs retained mammary epithelial cell characteristics and secreted β-caseins even when grown on plastic substratum. Thus, the established cell line had normal morphology, growth characteristics, as well as secretory characteristics, and it could be considered as a model system and useful tool for understanding the biology of dairy cow mammary glands.
Xu et al., P. (2020). In vitro culture and evaluation of bovine mammary epithelial cells from Ukraine dairy cows. Iranian Journal of Veterinary Research, (), -. doi: 10.22099/ijvr.2020.37714.5508
MLA
P. Xu et al.. "In vitro culture and evaluation of bovine mammary epithelial cells from Ukraine dairy cows". Iranian Journal of Veterinary Research, , , 2020, -. doi: 10.22099/ijvr.2020.37714.5508
HARVARD
Xu et al., P. (2020). 'In vitro culture and evaluation of bovine mammary epithelial cells from Ukraine dairy cows', Iranian Journal of Veterinary Research, (), pp. -. doi: 10.22099/ijvr.2020.37714.5508
VANCOUVER
Xu et al., P. In vitro culture and evaluation of bovine mammary epithelial cells from Ukraine dairy cows. Iranian Journal of Veterinary Research, 2020; (): -. doi: 10.22099/ijvr.2020.37714.5508