In vitro culture and evaluation of bovine mammary epithelial cells from Ukraine dairy cows

Document Type : Full paper (Original article)

Author

College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Eastern Hua Lan Street, 453003 Xinxiang, China

10.22099/ijvr.2020.37714.5508

Abstract

Background: Mammary epithelial cells have been widely-used over the years as models to understand the physiological function of mammary disease. Aims: This study aimed to establish a culture system and elucidate the unique characteristics of bovine mammary epithelial cells (BMEC) from the milk of Ukraine Holstein dairy cows in order to develop a general in vitro model. Methods: The milk from a three year old lactating dairy was used as a source of the epithelial cell, Characteristics of BMECs were examined using real-time cell assay (RTCA), immunocytochemistry (ICC), Reverse Transcription-Polymerase Chain Reaction (RT-PCR) and Westren blot (WB). Results: The results showed that bovine mammary epithelial cells can be recovered from milk, grown in culture, and exhibit the characteristic cobblestone morphology of epithelial cells. Conclusion: The established BMECs retained mammary epithelial cell characteristics and secreted β-caseins even when grown on plastic substratum. Thus, the established cell line had normal morphology, growth characteristics, as well as secretory characteristics, and it could be considered as a model system and useful tool for understanding the biology of dairy cow mammary glands.

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