Characterization of β-lactamase and quinolone resistant Clostridium perfringens recovered from broiler chickens with necrotic enteritis in Bangladesh

Document Type : Full paper (Original article)

Author

Animal Health Research Division, Bangladesh Livestock Research Institute (BLRI), Savar, Dhaka 1341, Bangladesh

10.22099/ijvr.2020.36848.5376

Abstract

Background: Clostridium perfringens causes necrotic enteritis (NE) and is considered a major economic burden in broiler industry and a significant foodborne pathogen, worldwide. Characterization of C. perfringens isolated from NE affected broiler chickens with the presence of β-lactamase and quinolone resistant genes were investigated in this study. Methods: Total 224 intestinal and caecal specimens were collected from NE affected broiler chickens and cultured to isolate C. perfringens. The toxicogenic characterization of C. perfringens was appraised using PCR and antibiotic susceptibility testing (disc diffusion method). The select C. perfringens isolates were characterized for β-lactamase and quinolone encoded genes by PCR analysis. Results: All isolates were cultured positive for C. perfringens and identified α-, β-, β2-, ε-, ι-, withenterotoxin. About 65.6% isolates had a multidrug resistant profile but none of these isolates were resistant or susceptible to all screened antibiotics. A subset of isolates, 160 and 98 were analyzed for β-lactamase and quinolone genes respectively and recognized blaTEM, blaSHV and blaOXA in 64 (40%; CI 32.35-48.03%; p<0.001) isolates and qnrB and qnrS in 28 (28.57%; CI 19.90-38.58%; p<0.001) isolated except qnrA. Conclusion: Therefore, the isolates of C. perfringens were toxicogenic and carrying β-lactamase and quinolone resistance genes. Now a day, rational use of antibiotics and safe production of broiler chickens is the major concern to save public health.

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